Anti-inflammatory and Anti-oxidant Effects of PHBV/Collagen (PHCP) on Lipopolysaccharide-Induced Skin Inflammation

Jin-Yi Han3*, Xu Zi Guang, Jyung-Sik Kwak, Ki-Wan Oh, Han-Ik Bae 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

The anti-inflammatory effect of PHBV/Collagen (PHCP) was examined in a mouse model of lipopolysaccharide (LPS)-induced skin inflammation. Vascular permeability on the back skin was measured by the local accumulation of Evan’s blue dye after subcutaneous injection of LPS (30 μg site-1). Dye leakage in the skin showed a significant increase at 2 h after injection of LPS. This LPS-induced dye leakage was also completely inhibited by HO-1 inhibitor, ZnPP, and antioxidants, including methyl gallate, trolox, and mannitol. To study the possible mechanisms underlying the in vivo anti-inflammatory effect of PHCP against LPS-induced inflammation, we also examined the effects of PHCP on malondialdehyde (MDA) and glutathione levels in skin tissues and found that pretreatment with PHCP resulted in inhibited MDA elevation and a remarkable reduction of glutathione level. In addition, similar results were obtained after pretreatment with antioxidants, including trolox and mannitol, and HO-1 inhibitor, ZnPP. Histopathologically, an influx of neutrophils into the skin dermis was detected between 24 h and 72 h after LPS injection (30, 100 μg site-1), compared to control animals after injection of saline. This increase was greater in mice treated with 100 μg of LPS than in those treated with 30 μg of LPS and was significantly suppressed by pretreatment with PHCP, antioxidants, and HO-1 inhibitor. These results collectively suggest that PHCP has an anti-inflammatory effect against LPS-induced inflammation model in vivo and may be a good candidate for the skin tissue engineering biomedical application primarily through manipulation of the redox state.

경제적 부가가치의 유용성에 관한 연구

한광식,서진원 단국대학교 경영경제연구소 2000 經營, 經濟硏究 Vol.2 No.-

Value of an enterprise can be represented by its market share, net sale and stock price etc. Among them stock price can be considered as representative index reflecting actual value of an enterprise. For this reason there should be adequate compensation to the investors for their investment. Under such consept EVA(Economic Value Add) is calculated by subtracting the cost of capital from the NOPAT(Net Operating After Tax). The aim of this study is to express effectiveness of EVA theoretically and indentify its effectiveness as an explanation for stock value (Cummulated Abnormal Return : CAR). This study show theoretically the greatest of utility of equity and harmony of executive and shareholders. the method of the empirical study is the regression analysis. therr are a dependent variable is CAR, independents variables are Price Earing Ration(PER), Return On Equity(ROE), Return on Asset(ROA), Return On Sales(ROS), EVA and Equity Ratio(ER). This study is to analysis of the study is that EVA is useful to information users. In order to test the above major hypothesis, the following minor hypothesis is divided. H₁ : EVA have information added for CAR. H₂ : EVA is no difference between brisk market and recessed market period. In conclusion, We have found the following though the empirical study. Result of analysis showed that EVA has overall effectiveness as an explanation on stock value (CAR) on stocks and it is identified that investors are making their investment after receiving advance information on difference between investment profitability and cost of capital. Analysis is made by dividing the period into brisk market and recessed market period. During the brisk market period EVA is appeared to have significant effectiveness as explanation on stock value(CAR), but during recessed market period it appeared to have no such significance as effective explanation. It is shown during brisk market period investment information on the difference between profitability rate and cost of capital rate is furnished in advance and being reflected to decision making by investors. And such result comes from the fact that during the period of recessed market due to presence of mixed information over-reaction of the investors and difference among enterprises with respect to its calculation of capital is deepened. For the purpose of increasing the value of an enterprise more effort shall be made to establish the composition ratio between own capital and other's capital properly and increase ratio of investment profitability to a level higher than cost of capital to bring about value of enterprise.

合倂成果에 대한 實證硏究 : 財務比率分析을 중심으로

韓光植,金鎭錫 단국대학교 산업연구소 2000 산업연구 Vol.22 No.-

The purpose of this study attempted to have empirical analysis on financial performance of our listed enterprises on the basis of comparison between it financial status before and after M&A from 1986 to 1995 were organized as at) test group and another group of 24 enterprises were made into a control group and by means of paired sample difference in average between t재 groups during the period of 2 years before the M&A and 2 years thereafter was computed and then net M&A effect was produced by deducting difference in average between the test group and control group and resulting figures were subjected to test and Wilcoxon Signed-Rank Test. As a resulted of this study it could be discovered that enterprises in Korea which underwent M&A did not show significant difference in any financial performance after M&A but only showed significant difference financial performance after M&A but only showed significant difference in performance in market value ratio and earning ratio for stocks (PER). Also based on M&A types any significant improvement in financial performance appeared more frequently with conglomerate M&A than with non-conglomerate case solely in quick ratio and net sales growth ratio but there was no significant difference in performance in terms of financial ratio. From this study it was possible to confirm that there was no significant difference in financial performance through M&A of our enterprises but some difference in stock earning ratio (PER) thus showing that our enterprises which went through M&A rae receiving positive appraisal from our capital market. And it was also discovered that while there was no significant difference in financial performance when analysis on the basis of M&A types but some significant financial performance in terms of quick ratio net sales growth ratio in conglomerate M&A.

강우시 채수빈도가 논 오염부하량 산정에 미치는 영향

한국헌,김진호,이종식,이정택,조재영,윤광식 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

한정된 수질자료를 이용 오염부하 산정시 집중적으로 많은 샘플을 채취한 경우에 비해 어느 정도 차이를 갖는지를 규명하는 것은 모니터링에 따른 오염부하량 산정결과의 신뢰 범위 파악뿐만 아니라 경제적인 수질 샘플 채수빈도 수립에 곡 필요한 것으로 사료된다. 그 결과를 요약하면 다음과 같다. 논에서의 강우-유출시 채수 빈도가 오염부하 산정에 미치는 영향을 파악하기 위해 9개의 강우사상에 대해 2시간 간격으로 연속 수질샘플을 실시하여 분석한 결과 전체적으로 강우-유출과정 중 T-N, T-P, SS 농도는 시간별로 증·감변동을 하였으나 유량과의 상관성은 나타나지 않았다. 수질샘플 횟수에 의한 영향은 강우-유출과정 중 5회 정도 샘플을 실시하면 많은 수의 샘플을 채수한 경우에 비해 T-N ±15.2%, T-P는 -20.0~26.2%, SS는 -28.6~35.7% 범위안에서 오염부하를 추정할 수 있는 것으로 나타났으며, 차이범위가 ±10% 내에 들어가기 위한 샘플수는 일주기 조사시 T-N, T-P의 경우는 6회, SS의 경우는 단기유출시는 4회 정도 실시하면 되고, 장기 유출시에는 11회 정도 실시하면 되는 것으로 조사되었다. 유량-유하부하량 관계식으로 ±10%내의 차이를 보일 샘플횟수는 T-N의 경우는 6회 이상, T-P와 SS는 9회 이상 정도 실시하면 되는 것으로 조사되었다. In order to examine effects of sampling frequency during rainfall-runoff process from paddy field on the estimation of pollution load, EMCs of several water sampling frequencies were examined. Water quality samples were conducted by every two hours interval for each event. It was found that difference of load estimation between five times sampling and two hours consecutive sampling during rainfall-runoff showed 15.2~-15.2% for T-N, 20.0~-26.2% for T-P, 28.6~-35.7% for the SS, respectively. In the same way, the effects of number of sampling data on estimation of pollution load using runoff-mass load(L-Q) method were investigated. L-Q equation made of five times sampling data provided 10% differences in estimation of mass loads of T-N, T-P, and SS when compared to those by L-Q equation using entire two hours consecutive sampling data during runoff process.

융모성림프구를 보이는 비장변연부 림프종 1예

진한영,손창학,주영돈,박정하,이재익,조영완,이원식 인제대학교 2006 仁濟醫學 Vol.27 No.-

Splenic marginal zone lymphoma is very rare B-cell lymphoma, characterized by an indolent clinical course. Clinical features are moderate-to severe splenomegaly, absolute lymphocytosis, and bone marrow intrasinusoidal infiltration of lymphocyte. We report the case of a 37-year-old male with SMZL. He complained of LUQ pain, who had a massive splenomegaly and moderate lymphocytosis in peripheral blood. Immunophenotyping findings and morphologic findings were consistent with SMZL. He underwent splenectomy and received chemotherapy with cyclophosphamide for eight months. Now, one year later after surgery he has a nearly normal blood count with no treatment.

철봉 어깨 틀어 휘돌며 다리 모아 엔도(Endo) 동작의 운동학적 분석

한윤수,백진호,이용식 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

This study was attempted to kinematical characteristics of the Endo El-grip Swing on the horizontal bar. At 2002 Busan Asian Games, three endo piked in el-grip swing were filmed using two video cameras during men's team competition. The three dimensional motion analysis with DLT method was executed using two video cameras of analyzing the actual competition situation. It was summarized that (a) It was indicated large hip and shoulder angle at E1, small hip angle and large sholder angle from E1 to E2, the smallest hip angle at E3, flexional hip and shoulder angle from E3 to E4, large hip and shoulder angle at E5; (b) It was indicated large angular velocity of hip at E3, large angular velocity of trunk rotation from E2 to E4, small angular velocity of shoulder and trunk rotion.

白鼠의 末梢 및 中樞 인슐린/도파민 調節樣相에 關한 硏究

韓辰熙,李定均,金容植 서울醫大 1991 精神醫學 Vol.16 No.2

Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells

Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4

The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.

5-Fluorouracil 및 mitomycin C가 지라조직에 미치는 영향

고정식,안의태,박경호,김진국,김의한,정영신 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.2

동축선로형 λ/4초크 대역억제필터의 최적화

정봉식,이규한,김진길 東亞大學校 附設 情報通信硏究所 1999 情報通信硏究所論文誌 Vol.7 No.1

In this paper the band-stop filter that rejects the undesired 3th and 5th harmonics generated from high frequench generator is analyzed and optimized. The length of the band-stop filter is setup initially a quarter of a wavelength fo the harmonic, but initial filter structure is unable to cutoff the harmonics because of the fringing effect at the input of the choke. To reduce the mismatch between the cutoff frequency and the harmonics, the length of the choke is optimized by using iterative Levenberg-Marquardt Algorithm.