백서에서 실험적 치아이동시 치조골 흡수에 미치는 Bisphosphonate의 영향 : Biochemical & Histological Observations

최진휴,김종철 전남대학교 치과대학 1998 전남치대논문집 Vol.10 No.1

This study was performed to examine the effect of bisphosphonate, an inhibitor of bone resorption, on the formation of osteoclast and bone resorption during experimental tooth movement. Whether bisphosphonate has a cytotoxicity in high dose was also examined. Eighty-seven male Sprague-Dawley rats, weighing 260-350g, were classified into normal (no appliance + 0.9% NaCl), control (appliance + 0.9% NaCl) and four bisphosphonate-treated (appliance + 0.8, 4, 20 or 100 ㎎/㎏) groups. The maxillary left first molar was moved mesially with the tipping movement using 50-70g of force. Bisphosphonate(etidronate disodium) was injected intraperitoneally with a dose of 0.8, 4, 20, or 100 ㎎/㎏ simultaneously with the application of the orthodontic force. They were killed at day 1, 3, or 7 after the application of the orthodontic force. The activities of serum acid phosphatase and lactate dehydrogenase (LDH) were assayed, and osteoclasts and the degree of bone resorption were examined histologically. The results obtained were as follows: 1. Acid phosphatase activities were significantly higher in the appliance groups, both control and bisphosphonate-treated (4, 20, and 100 ㎎/㎏) groups, at day 1 and 3 than these in normal. At day 1, bisphosphonate-treated(4, 20㎎/㎏) groups showed even higher acid phosphatase than control. However, at day 7, no significant difference was noted between the control and bisphosphonate-treated groups. 2. LDH activities in the 4, 20㎎/㎏ bisphosphonate-treated groups were increased during the experimental periods examined, but there were no significant differences in the 0.8, 100㎎/㎏ bisphosphonate-treated groups. 3. There was no bone resorption at day 1, but severe bone resorption was observed at days 3 and 7 in the control. Bone resorption was reduced by bisphosphonate-treatment at day 3. Bone resorption observed at day 7 was similat between the control and bisphosphonate-treated groups. 4. Few osteoclasts were observed at the alveolar bone in the control and bisphosphonate-treated groups at day1. At day 3, numerous osteoclasts were shown in the control, the degree of which was reduced in bisphophonate-treated groups. These results suggest that the inhibition of the osteoclast formation was not the mechanism of bone resorption by the bisphosphonate-treated during experimental tooth movement. There was no distinct cytotoxicity with a high dose of bisphosphonate. And the drug should be administrated repeatedly to maintain the inhibitory effect of bone resorption.

혼합유의 이화학적 특성에 관한 연구

최성희,박철진,오성기 경희대학교 부설 식량자원개발연구소 1992 硏究論文集 Vol.13 No.-

The objective of this study was to investigate the physicochemical characteristics of binary and ternary oil systems. Materials used in this experiment were four edible oils i. e. sesame, soybean, red-pepper seed and Sancho (Zanthoxylum schinifolium) seed oils. The physicochemical properties of mixed oils, as well as the inductive period of oxidation, were measured. The results obtained are as follows : 1. The optimum mixing ratios of sesame oil with each of soybean, red-pepper seed and Sancho seed oils, which still maintained a characteristic sesame oil flavor, were 1 : 3,1 : 0.5 and 1 : 0.5, respectively. 2. Sesame, soybean and red-pepper seed oils showed low acid and peroxide values. In contrast, however, Sancho seed oil showed the very high values, and acid, peroxide and TBA values were 17. 4, 28.6 and 1.8, respectively, Among the oils, soybean oil gave the longest induction period of oxidation and Sancho seed oils the shortest, and the induction period was increased in order of Sanch seed, red-pepper seed, sesame and soybean oils. 3. Binary oil system such as a mixed oil of seasame oil and each of soybean and red-pepper seed oils did not show any significant change in acid, peroxide and TBA values, but a mixed oil of sesame oil and Sancho seed oils gave a drastic change in acid (7.2), peroxide (12.3), and TBA (0.2) values. All the binary oil systems extended their induction periods of oxidation. 4. In ternary oil system, a mixed oil of sesame, soybean and red-pepper sees oils did not show any significant change in acid and peroxide values. In contrast, however, mixed oils of sesame, red -pepper seed and Sancho seed oils in the ratios of 1 : 1 : 1 and 4.5, 8.9 and 0.13 in the ratio of 1 : 0.25 : 0.25, respectively. All the ternary oil systems also extended the induction period of oxidation.

단기간 근권 저온처리가 수경재배 상추의 질산태 질소 함량에 미치는 영향

최승주,양진철,사동민 한국환경농학회 2002 한국환경농학회지 Vol.21 No.1

질산태 질소의 일중 농도 변화와 단기간의 저온 처리가 질산태 질소의 흡수 및 농도에 미치는 영향을 살펴보고자 상추(Lactuca sativa L.)를 공시 작물로 하여 온실에서 수경 재배하였다. 질산태 질소의 농도는 염산에 비하여 주맥에서 일중 지속적으로 2배 이상 높았으며, 질산태 질소의 일중 변화는 14:00까지 지속적으로 감소하여 최저치 (8.7 mg-N/GDW)를 나타낸 후 다시 증가하였다. 질산태 질소의 일중 흡수량은 11:00~17:00에 평균 4.8mg-N/GDW-Root/hr로 최대치를 나타내었다. 단기간의 저온 처리량을 50%~55%를 감소시켰다. 이러한 결과는 수확 전 단기간의 저온처리가 상추 잎의 질산태 질소함량을 낮출 수 있는 방법으로 응용될 수 있음을 보여준다. Lettuce (Lactuca sativa L.) plants were grown under hydroponic system to characterize the diurnal change of nitrate concentration and nitrate uptake rate and to examine the effect of short term cold treatment to rhizosphere on nitrate concentration and uptake rate in lettuce plant. The nitrate concentrations in midrib were two times higher than those in leaf. Nitrate concentration in the shoot reached to minimum (8.7 mg-N/GDW) at 14:00 and, thereafter, increased continuously until 23:00. During 11:00~17:00, nitrate uptake by lettuce plant was maximum (4.8 mg-N/GDW-Root/hr). Short term cold treatment reduced nitrate concentration in the shoot by 14~18%, and nitrate uptake rate by 50~55%, respectively. These results showed that short term cold treatment before harvest could be applied for the purpose of reduction of nitrate concentration in the leaf under hydroponic culture.

Goretex를 이용한 융비술

최진영,민철기,명훈,황순정,서병무,이종호,정필순,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

융비술시 실리콘 고무는 다루기 쉽고 조직 반응이 적어 현대 많이 돌출의 합병증이 생길 수 있다. 저자등은 이러한 실리콘의 단점을 보완할 수 있는 goretex를 사용하여 융비술을 시행하고 비첨에 대해서 자가연골을 이식하여 만족할 만한 결과를 얻었기에 goretex가 안면부 연조직의 증강을 위한 이식재료로 적합하다고 생각되어 문헌고찰과 함께 보고하는 바이다. Many different materials have been used to provide augmentation of the nasal dorsum. this include both autogenous and homogenous materials,as well as allografts. Silicone among the artificial materials has been most widely used. The silicone has some advantages and disadvantages. Goretex(expanded polytetra-fluoroethylene) which has widely used in vascular surgery and abdominal surgery can be used for facial soft tissue augmentation. The author presemts 2 cases of nasal augmentaion using goretex with literature review.

Homocysteine과 대사증후군의 관계

최진욱,김선현,염창환,정규철 관동대학교 의과학연구소 2006 關東醫大學術誌 Vol.10 No.1

회야강(울산)의 식물플랑크톤 군집구조

최철만,배진현 한국환경과학회 2002 한국환경과학회지 Vol.11 No.3

This study was conducted to find the structure of phytoplankton community in the Hoeya river(Ulsan) from March 2000 to February 2001. The taxa of phytoplankton identified included 135 species, 5 divisions, 33 families and 82 genera. Among them, chrysophyta(diatoms) were 63 species(46.7%), chlorophyta(green algae) 47 species(34.8%), pyrrophyta(dinoflagellates) 13 species(9.6%), cyanophyta (cyanobacteria) 8 species(5.9%) and euglenophyta(euglenoids) 4 species(3.0%) respectively. Ecological important species is called frequently appearing species, red tide causative species, seawater species and pollution indicator. 22 species including Aulacoseira granulata were recorded frequently appearing species. 19 species including Ceratium furca were recorded as red tide causing species. And the pollution indicators were 33 species including Actinastrum hantzschil var. fluviatile. The highest standing crops were 3,103,441 cell/ℓ in August at the RW-1 and the lowest 1,245 cell/ℓ in January at the RW-5. In the community analysis, the dominanance indices ranged from 0.34(October, RW-2) to 0.94(January, RW-1) and the diversity indices from 0.50(May, RW-2) to 2.57(September, RW-2). The saprobic indices were 2.43 in RW-1, 2.41in RW-2, 2.375 in RW-3, 2.40 in RW-4, 2.43, in RW-5. Therefore, these areas were investigated "β-mesosaprobic". According to the similarity index among the stations, these areas were defined as residential district areas(RW-2 and RW-3), lower part of the dam (RW-1) and seawater areas(RW-4 and RW-5).

백서에서 실험적 치아이동시 치조골 흡수에 미치는 Bisphosphonate의 영향 : 생화학적 및 조직학적 관찰 Biochemical & Histological Observations

최진휴,김종철 대한치과교정학회 1999 대한치과교정학회지 Vol.29 No.1

백서에서 실험적 치아 이동시 bisphosphonate가 파골세포의 형성에 미치는 영향과 골 흡수 억제기전을 규명하고 독성유무를 알아보고자 하였다. 체중 260-350g의 웅성 백서 87마리를 정상군(장치비장착 + 0.9% NaCl), 대조군(장치장착 _ 0.9% NaCl) 및 장치장착후 bisphosphonate 투여군(0.8mg, 4 mg, 20 mg, 및 100mg/kg) 으로 분류하였다. 상악 좌측 제1대구치를 근심으로 치아이동이 일어나도록 50-70g의 교정력을 가하고, 교정장치 장착후 1일, 3일 및 7일째에 혈청 acid phosphatase와 lactate dehydrogenase (LDH)의 활성도를 측정하고, 또한 제1대구치를 포함한 상악골일부에서 파골세포수 및 골흡수 정도를 조직학적으로 관찰하여 다음과 같은 성적을 얻었다. 1. Acid phosphatase 활성도는 장치후 1일째와 3일째에 대조군과 bisphosphonate 투여군에 서 모두 정상군에 비해 2-3배 높았으나, 7일째에는 정상군과 유의한 차이를 보이지 않았다. 2. LDH활성도는 bisphosphonate 4 mg과 20mg/kg 투여군에서 전 실험기간에 걸쳐 증가된 양상을 보였으나 0.8mg과 100mg/kg 투여군에서는 유의한 차이를 나타내지 않았다. 3. 골흡수는 장치후 1일째에 대조군과 bisphosphonate 투여군에서 모두 관찰되지 않았으나, 3일 이후에 나타난 7일째가지 지속되었다. Bisphosphonate 4, 20 및 100mg/kg 군에서의 골흡수정도는 3일째에는 대조군에 비해 미약하였으나 7일째에는 대조군과 유사하게 나타났다. 4. 파골세포는 1일째에 대조군이나 bisphosphonate 투여군 모두에서 거의 관찰할 수 없었다. 3일째에 대조군에서는 파골세포가 다량 출현하였으나 bisphosphonate 투여군에서는 약물의 용량이 증가함에 따라 감소하여 나타났다. 이상의 결과로 실험적 치아이동시 파골세포의 형성억제가 bisphosphonate에 의한 골흡수억제기전이 아님을 알 수 있었고, bisphosphonate는 투여량이 증가에 따른 뚜렷한 세포독성은 관찰되지 않았으며, 골흡수 억제효과를 지속시키기 위해서는 약물이 반복적으로 투여되어야 할 필요가 있음이 시사되었다. This study was performed to examine the effect of bisphosphonate, an inhibitor of bone resorption, on the formation of osteoclast and bone resorption during experimental tooth movement. Whether bisphosphonate has a cytotoxity in high dose was also examined. Eighty-seven male Sprague-Dawley rats, weighing 260-350g, were classified into normal (no appliance + 0.9% NaCl), control (appliance + 0.9% NaCl) and four bisphosphonate-treated (appliance + 0.8, 4, 20, or 100mg/kg) groups. The maxillary left first molar was moved mesially with the tipping movement using 5-70g of force. Bisphosphonate(etidronate disodium) was injected intraperitoneally with a dose of 0.8, 4, 20 or 100mg/kg simultaneously with the application of the orthodontic force. They were killed at day 1, 3 or 7 after the application of the orthodontic force. The activities of serum acid phosphatase and lactate dehydrogenase (LDH) were assayed, and osteoclasts and the degree of bone resorption were examined histologically. The results obtained were as follows: 1. Acid phosphatase activities were significantly higher in the appliance groups, both control and bisphosphonate-treated (4, 20, and 100 mg/kg) groups, at days 1 and 3 than these in normal. At day 1, bisphosphonate-treated(4, 20mg/kg) groups showed even higher acid phosphatase than control. However, at day 7, no significant difference was noted between the control and bisphosphonate-treated groups. 2. LDH activities in the 4, 20mg/kg bisphosphonate-treated groups were increased during the experimental periods examined, but there were no significant differences in the 0.8, 100mg/kg bisphosphonate-treated groups. 3. There was no bone resorption at day, 1, but severe bone resorption was observed at days 3 and 7 in the control. Bone resorption was reduced by bisphosphonate-treatment at day 3. Bone resorption observed at day 7 was similar between the control and bisphosphonate-treated groups. 4. Few osteoclasts were observed at the alveolar bone in the control and bisphosphonate-treated groups at day 1. At day 3, numerous osteoclasts were shown in the control, the degree of which was reduced in bisphosphonate-treated groups. These results suggest that the inhibition of the osteoclast formation was not the mechanism of bone resorption by the bisphosphonate-treatment during experimenal tooth movement. There was no distinct cytotoxicity with a high dose of bisphosphonate. And the drug should be administrated repeatedly to maintain the inhibitory effect of bone resorption.

Molar Uprighting Spring에 의해 발생되는 치조골내의 응력분포에 관한 광탄성학적 연구

최진휴,김종철 전남대학교 치과대학 1990 전남치대논문집 Vol.2 No.1

This study was performed to analyze the effects of forces to the alveolar bone by various molar uprighting spring such as helical uprighting spring, T-loop spring, Modified T-loop spring and open coil spring. The simplified two-dimensional photoelastic model was constructed with a lower left posterior quadrant containing the second molar, the first and second premolars and the canine, with the first molar missing. Several molar uprighting springs were fabricated from 0.017 by 0.022 inch blue Elgiloy and applied to the photoelastic model. Two-dimensional photoelastic stress analysis was performed, and the stress distribution was recorded by photography. The results obtained were as follows ; 1. In all the kinds of the springs, the center of rotation of the mandibular second molar was observed at the apical 1/5-1/6 between the alveolar crest and the root apex. 2. In all the kinds of the spring, the stress induced in the mesial root surface of the manibular second molar was relatively homogeneous but there was some difference in the magnitude of the stress. 3. In the kinds of the springs, the distal crown tipping moment of the second molar was increased in turn as open coil spring, helical uprighting spring, T-loop spring, and modified T-loop spring. 4. The largest extrusive force was occured in the T-loop spring, intrusive force was occured in Modified T-loop spring only, and the largest distal tipping force was occured in open coil spring. 5. In the T-loop spring with activation, the stress induced in the mesial root surface of the second molar was increased gradually from the root apex to the alveolar crest and highly concentrated in the alveolar crest.

入口에 案內管이 있는 開放型 熱 사이폰에 關한 硏究

최인규,이진호,김철주 연세대학교 대학원 1976 延世論叢 Vol.13 No.2

The experimental study was carried out to investigate the characteristics of the flow fluctuation pheonomena, which occurred in the transition process from the impeded flow regime to the boundary layer flow regime in awl open thermosyphon by placing flow guides at the center of the orifice inlet where fluctuation occurred. The diameter and length of the guides were varied and their effects were investigated. With guides of long length, the fluctuation was not observed, and of medium length, the fluctuation was observed only in case of large diameter. However, when the length of the guides were short, the fluctuation took place just like in case of no guides. In this case, the existance of upward flow in the guide just before fluctuation occurred, resulted in reverse flow of the thermosyphon, however the Nusselt number showed same characteristics when the temperature of the orifice inlet was taken as the thermosyphon outlet temperature instead of as inlet temperature.

蓄熱用 TMA 包接化合物의 熱物性에 關한 實驗 硏究(Ⅰ)

최영범,한영옥,하옥남,김병철,김진흥 조선대학교 동력자원연구소 1996 動力資源硏究所誌 Vol.18 No.2

This paper presents the test-tube experimental results of thermal properties of TMA clathrate compounds for latent heat storage applications. The results are : ⅰ) subcooling in TMA clathrate compound is the lower than water, ⅱ) Phase change temperature in TMA clathrate compound is the higher than water. ⅲ) Latent heat of 25wt% TMA clathrate compounds of 220.4kJ/㎏K appear to be maximum. ⅳ) Comparing with water, volume of TMA clathrate compounds decreased as the phase change. ⅴ) TMA clathrate compounds precipitated because of increase of density as phase change. Thus, TMA clathrate compounds are found to be the promising compounds for the heat storage from the viewpoint of thermal property.