Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

단순반복 작업자의 작업대 및 의자의 형태와 누적외상성질환 증상과의 관계

정현기,노영만,임현우,박정일,정춘화 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

목적 : 단순반복작업 생산직 근로자의 누적외상성질환에 대한 사업주의 건강관리규정 준수상태 및 근로자의 자각증상호소와 의자 및 작업대의 형태를 파악하고 크기를 측정하여 이들과 누적외상성질환 자각증상호소와의 관련요인을 알아보고 대책을 제공하고자 한다. 방법 : 서울 및 경기지역 일부 100인 미만 사업장중 전기, 전자부품 제조업체 7개를 선정하여 생산직 근로자 77명을 대상으로 누적외상성질환에 대한 사업주의 건강관리규정 준수상태와 및 근로자의 자각증상호소에 대한 설문조사를 실시하고 의자와 작업대의 크기를 측정하고 증상과의 관련요인을 알아보기 위하여 로지스틱회귀분석을 실시하였다. 결과 : 의자와 작업대가 작업자세에 영향을 준다고 인식하는 작업자는 85.7 %이였으며 단순반복작업 근로자 작업관리지침에 의한 사업주의 근로자에 대한 관심은 매우 낮았다. 누적외상성질환 자각증상 호소율은 63.6 %이였으며 자각증상 부위는 어깨 37.9%, 목 15.2 %, 등 6.1 %, 팔/팔꿈치 4.6 % 순이였다. 입사 후 초기발생시기는 평균 16.5개월이며 자각증상 호소는 작업대 두께, 작업대높이, 작업대 밑무릎공간, 등받이 높이, 좌석면 높이, 좌석면의 앞뒤길이, 좌석면의 좌우길이, 작업대끝에서 어깨까지의 거리에서 유의한 관련이 있었다. 자각증상호소의 관련요인은 등받이 높이, 좌석면의 높이, 좌석면의 앞뒤길이 이였다. 결 론 : 이상의 결과로 보아 정부는 단순반복작업 작업자의 누적외상성질환 예방을 위하여 사업주에 대 한 철저한 교육 및 관리감독을 해야한다. 사업주는 작업대 및 의자를 작업자의 신체치수와 작업내용에 맞게 높이를 조절할 수 있는 제품을 사용하고 작업전, 후에 정기적인 건강체조 및 휴식을 갖도록 하며 근로자에 대한 실질적인 보건교육이 이루어져야 한다. Objectives : The main purpose of this study was to investigate relationship between cumulative trauma disorders (CTDs) and the types of workstation and chair in workers with repetitive motion tasks. Methods : The study subjects were 77 repetitive motion tasks workers at small manufacturing industries in Seoul and Gyung-gi district area. A questionnaire was used to obtain about health practice for prevention and prevalence of CTDs by worker. The type of Workstations and chairs in workers were measured and analyzed with prevalence of CTDs by logistic regression. Results : Eighty-six percent of workers recognized that work posture was related to workstation and chair, Most employers and employees didn't follow the work practice guidelines for workers with repetitive motion tasks. The prevalence of CTDs was 64% by questionnaire. The prevalence of pain in shoulder, neck, back, and arm were 38%, 15%, 6%, and 5%, respectively, and the pain started after working an average duration of 16.5 months. The CTDs symptoms were significantly related to thickness of workstation, height of workstation, length of knee depth under workstation, height of back rest, height of seat pan, depth of sitting surface, width of sitting surface, and length from workstation surface edge to shoulder. By multiple regression analysis of each risk factor, prevalence of CTDs was highly correlated with height of back rest, height of seat pan, and depth of sitting surface. Conclusions : This study suggests that workstations should be made adjustable for height and chairs should be equipped with seat pan height control, enough sitting surface for depth and width, back rest height and angle control. The workers should use the right working postures, exorcise for health, occupational health education, and rest breaks and the manager of company should be educated for the preventing of CTDs.

근관충전용 글라스 아이오노머 시멘트의 근단 폐쇄성과 치질 적합성에 관한 연구

김현아,최기운 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2

The purpose of this study was to compare the apical leakage and adaptation to the dentinal wall of a glass ionomer root canal cement(Ketac-Endo) with those of AH-26 and Tubli-Seal. 102 single-rooted teeth were insrumented with step-back technique and randomly divided into four groups according to kinds of root canal cements: Ketac-Endo, AH-26, Tubli-Seal, and no root canal cement. Four experimental groups were as follows; Group 1:Filling with gutta-percha and Ketac-Endo Group 2:Filling with gutta-percha and AH-26 Group 3:Filling with gutta-percha and Tubli-Seal Group 4:Filling with gutta-percha without root canal cement All the specimens were obturated with lateral condensation technique, and stored in 100% humidity for 4 days. 20 teeth in each group were placed in centrifuge tube filled with India ink, and then centrifuged for 20 minutes at 3,000 rpm. And they were evaluated for linear dye leakage using a stereoscope and analyzed by one-way ANOVA. 22 teeth were split longitudinally and evaluated the adaptation to the dentinal wall using scanning electron microscope(4 teeth in each group) and stereoscope(2 teeth in 1, 2, 3 group). The obtained results were as follows; 1. In case of not used root canal cement(Group 4), there was showed much degree of dye penetration comparerd with used root canal cements(Group 1, 2, 3)(P<0.01). 2. In the case of used root canal cements(Group 1, 2, 3), there was no significant difference in linear leakage in groups(P>0.01). 3. In the case of used root canal cements(Group 1, 2, 3), there was showed intimate adaptation to the dentinal wall independent on kinds of root canal cements. 4. Fractured surfaces indicated failure of cohesion in Ketac-Endo, and failure of adhesion to dentine in AH-26 and Tubli-Seal. 5. According to similar apical leakage and adaptability to the dentinal wall to another root canal cements, it is possible to be used glass-ionomer cements as root canal cement.

운동수요자들의 스포츠센타 프로그램 이용실태 조사분석 : Focused on mass sports facilities in the recreational sports center

김현권,배기열 한국학교체육학회 2001 한국학교 체육학회지 Vol.11 No.2

정보화사회로 접어들면서 과거와는 달리 고객감동 ·고객중심 고객만족을 외치는 기업들이 크게 늘어나고 있다. 이러한 변화의 양상은 고객에 봉사하기 위해 존재한다는 企業 본연의 자세를 망각해 온 것에 대한 자기 반성에 그 뿌리를 두고 있는 것으로 사료된다. 특히 고객 서비스에 대한 경쟁이 강화되면서 소비자들의 선택의 폭이 그만큼 커지게 되었을 뿐만 아니라 요즘의 어려운 경제상황 관리체제하에서 소비자들은 보다 내실 있는 소비를 하기 때문에 과거와 동일한 경영자세로는 시장에서 생존하기조차 어려운 상황이 초래되었다. 따라서 이런 무한 경쟁과 어려운 경제상황 관리체제하에서 많은 사회체육시설중 스포츠센타가 생존하기 위해서는 무엇보다 변화하는 회원의 욕구를 정확히 파악하는 동시에 운영의 경쟁력을 확보할 수 있는 장·단점의 분석을 통해 스포츠센타의 정확한 현재 모습을 파악하는 것이 급선무일 것이다. This study distribute composed questionaie of sum 22items(basic 6. program 16) about trends to program utilization of the masses sports facilities by interview. Total 650 members of membership using the masses sports facilities in social welfare foundation in order to know current trends to program utilization and service satisfaction of the masses sports facilities. Therefore through analysis as a whole verification frequency analysis by percentage and synthetic satisfaction index the resule of this response shows lilce the following. The current trends to program utilization of the masses sports facilities. 1) when program is participated considerable items importantly are contents, rental for and rental time 2) participation motivation of program took precedence health improvement 3) On the whole, program satisfaction degree was 85.7 percents. Judging from the truth of above matter, for using members of the masses sports facilities are prepared continuous guide, give more satisfactions more scientific, and systematic program development.

하악 제1 대구치 근단부의 형태학적 연구

정현,박상진,박상혁,최기운 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.2

The aim of this study was to investigate thc shapes and diameters of the physiological foramen and anatomy of the root canal at 3mm from apex in mandibular first molars. Slxty mandibular first molars were randomly selected. The apical anatomy of 60 mandibular first molars was investigated by means of a stereomicroscope (60x magnification). Thc results were as follows; 1 There was a high percentage of two physiological foramina in mesial (61.67%) and one foramen in distal (71.66%) roots of mandibular first molars. 2 There was a high frequency of accessory foramina in mesial roots with one foramen (26.07%) 3 The diameters of physiological foramen was as follows: ·0.329mm in single mesial foramen ·0.266mm in mesioccal foramen and 0.246mm in mesiolingual foramen ·0.375mm in single distal foramen ·0.291mm in distobuccal foramen and 0.237mm in distolingual foramen 4 The most common physiological foramen shape was oval (69.93%) 5 The incidence of isthmus in mesial root at 3mm from apex was 55%. The 3mm-sections contained a complete isthmus 31.66% and a partial isthmus 23.34% 6 3mm from tho apex, the most common canal shape was oval (50.64%) Knowledge of the apical anatomy of mandibular first molar would be necessary for success of surgical and nonsurgical endodontic treatment. 본 연구는 한국인의 하악 제1 대구치 60개를 대상으로 생리적 근단공의 크기와 형태, 근심 치근의 치근단 3mm에서 isthmus의 발생 빈도와 형태, 그리고 원심 치근의 치근단 3mm의 근관 형태를 입체현미경하에서 60배로 조사한 결과 다음과 같은 결론을물 얻었다. 1.근심 치근은 두 개의 근단공을 가지는 비율이 높았으며(61.67%), 원심 치근은 하나의 근단공을 가지는 비율이 높았다(71.66%). 2.부근단공의 빈도는 근심 치근의 1개의 근단공을 가지는 경우가 가장 높았다(26.07%). 3.생리적 근단공의 크기는 근심 치근에서 하나의 근단공을 가지는 경우 0.329mm, 근심 협측 근단공은 0.266mm 근심 설측 근단공은 0.246mm였으며, 원심 치근에서 하나의 근단공을 가지는 경우 0.375mm, 원심 협측 근단공은 0.291mm, 원심 설측 근단공은 0.237mm로 나타났다. 4.근단공의 형태는 oval 형태(69.93%)가 가장 많았다. 5.근심 치근의 치근단 3mm에서 isthmus의 발생빈도는 55%였으며, partial isthmus는 31.66%, complete isthmus는 23.34%로 나타났다. 6. 원심 치근의 치근단 3mm의 근관 형태는 oval 형태(50.64%)가 가장 많았다. 이상의 연구 결과 하악 제1 대구치의 성공적인 근관 치료를 위해서는 생리적 근단공의 실제 크기와 형태 및 isthmus의 존재에 대한 사전 지식이 필요할 것이다.

知的財産權을 目的으로 한 擔保權의 實行 : 民事訴訟法에 의한 實行 The Foreclosure by the Civil Procedure code

김기현 순천향대학교 사회과학연구소 1998 순천향 사회과학연구 Vol.4 No.1

As intellectual property is carrying great significance in the modern societies, it has turned out to be necessary that intellectual property should be accepted as collateral. The regulations on security interests, however, which were developed and institutionalized on the basis of tangible assets can not be applied to intellectual property because intellectual property, in essence, belongs to intangible assets. Since intellectual property belongs to intangible assets, the foreclosure of intellectual property is expected to be executed in accordance with the provision of Article 733 of the Civil Procedure Code. According to the Article, intellectual property belongs to non-tangible assets and comes under the regulations on obligatory execution. Intellectual property's distinctive quality of being intangible assets, however, prevents the regulations on obligatory execution from being applied to intellectual property. Thus this study aims to solve this problem caused by intellectual property's distinctive quality of being intangible.

電子化된 意思表示의 效力發生時期와 瑕疵 있는 到達의 危險負擔 : 電子郵便을 중심으로

金起顯 忠南大學校 法學硏究所 1997 法學硏究 Vol.8 No.1

SBR 반응조에서 슬러지의 침전특성에 관한 연구

김환기,이현기,박상민 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2002 工學硏究 Vol.33 No.-

Generally, in the sewage treatment sedimentation of sludge is very important. In this study SBR reactor sludge and input domestic sewage is used for sedimentation experiment. In the 2m depth, removal rate of domestic sewage is 20min. sedi.- 14.1%, 40min. sedi.- 26.7 %, 60min. sedi.- 34.4%, 90min. sedi.- 38.7%, 120min. sedi.- 40.8%, 150min. sedi.- 49.0%. Moisture content of SBR reactor sludge is over 99.2% and in the 1m 50㎝ depth moisture content is very quickly reduced. In the Sludge volume graph of SBR reactor sludge, because variation quantity of sludge volume is very few, we think proper sedimentation is 60 min.

知的財産權 擔保制度에 있어서 美國 聯邦法과 U.C.C.의 相衝

김기현 忠南大學校 法學硏究所 1999 法學硏究 Vol.9 No.2

한국의 여가학 연구 동향 분석에 관한 연구

김을교,김현기 명지대학교 예체능연구소 1995 藝體能論集 Vol.5 No.-

The Purpose of this Study is to find out current trends and problems of Leisure Studies from analysis of references which published in Korea from 1970 to June 1994, and to give some visions of Korean Leisure Studies development. The result of this study is as follows. 1. 961 Literatures related to leisure were published in Korea from 1970 to June 1994. 2. The major matters of interest of Leisure Studies in Korea is "Leisure Society", and it covers 51.7% of total researches. 3. Being divided from fields of Leisure Studies in Korea, 50.3% is thesis for a degree 47.8% is normal thesis, and the rest 2.0% is separated volumes. 4. The Leisure Studies in Korea has been magnificated from early stage of mere Physical Educaion to varieties of Studies, including, Studies of the old men, deucation, sociology, pshchology, studies of the Young, civil engineering, and studies sightseeing. 5. To develope Leisure Studies in Korea we need to increase quantity of Leisure research, to magnification our fields and territories of interest, and to correlate circumferent studies.