Study of decreased melanin production through p53 by heme oxygenase-1 inhibitor in normal human melanocytes

( Hee Sun Lim ),( Sun A Jin ),( Jee Bum Lee ),( Seong Jin Kim ),( Seung Chul Lee ),( Young Ho Won ),( Sook Jung Yoon ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Heme oxygenase (HO)-1 is induced by oxidative stress and plays important roles in anti-apoptosis and the rapid growth of several solid tumors. p53 has a central role in skin pigmentation and may impact onmelanoma at all stages. However, there has been little study of HO-1 in relation with p53 on melanin production Objectives: To know the effect of HO-1 on melanogenesis through p53 in normal human melanocytes Methods: Human melanocytes (hM) were primarily cultured from foreskin. After incubation, cells were rinsed twice with PBS then transfection with p53 siRNA Results: Melanin content was detected with ELISA and Western blot analysis and RT-PCR of tyrosinase, MITF were performed after ZnPP treatment and p53 transfection. After ZnPP treatment, melanin content was decreased, and tyrosinase and MITF protein levels were decreased. Tyrosinase and MITF mRNA levels were also decreased. However, melanin content and tyrosinase and MITF protein levels were increased after CoPP treatment. After p53 transfection, HO-1 expression was decreased when HO-1 stimulator was treated. In addition, melanin content was decreased, and tyrosinase expression was decreased in Western blot analysis Conclusion: These results suggest that melanogenesis is inhibited by ZnPP by decreased melanin production, tyrosinase and MITF protein and mRNA levels. Furthermore, those inhibitory effects of ZnPP may be dependent on p53 in normal human melanocytes. Therefore, HO-1 may play an important role in melanogensis

용융금속 이송용 전자기 펌프의 유동해석 및 실험

최재호(Jae Ho Choi),임효재(Hyo Jae Lim),김창업(Chang Eob Kim),권정태(Jung Tae Kwon) 대한기계학회 2007 대한기계학회 춘추학술대회 Vol.2007 No.5

This study aims at analyzing the flow characteristics of the electromagnetic pump using a linear induction motor (LIM) for transferring molten metals. The flow characteristics of the pump are simulated by magnetohydrodynamic(MHD) program. In this system, the LIM is used for transferring molten metal by electromagnetic force. The molten metal is treated as the secondary part of the LIM. Since the LIM produces an electromagnetic force in the duct, the molten metal can flow from the furnace to the reservoir. The flow characteristics of the pump are analyzed using MHD program for magnetic field of 0.1[T] in duct. In order to prove the analysis, we made a prototype electromagnetic pump using LIM.

A Study on the Design of Power System Stabilizer Using DeadBeat Control

Lim, Chan-Ho 慶州大學校 2005 論文集 Vol.18 No.2

데드비트제어는 리플이 없는 출려응답을 얻을 수 있고, 정해진 샘플링 주기 후에 오차 없는 정상상태에 도달하는 특성을 가지고 있다. 본 논문에서는 이러한 특성을 가진 데드비트 제어기를 전력계통 안정화 제어기에 적용하여, 전력시스템의 저주파 진동현상을 제어하기 위한 방안을 제안하였다 제안된 데드비트제어기는 적분제어기와 상태관측기 및 영차홀드로 구성하여 설계하였으며, 성능 검증을 위하여 최적 제어기와 비교 시뮬레이션을 수행하였다. 시뮬레이션 결과 초기상태에 의한 출력응답의 경우에 있어서 최적제어기 보다 1.5배 빠르게 정상상태에 도달하였고, 리플이 없는 응답을 보였다.

전통 사상 및 교육 방법의 현대 도덕 교육과정에서의 활용 가능성 연구

함규진 ( Kyu-jin Ham ),신창호 ( Chang Ho Shin ),임홍태 ( Hong Tae Lim ),지준호 ( Chun-ho Chi ) 한국철학사연구회 2016 한국 철학논집 Vol.0 No.50

도덕은 하나의 철학적, 심리적 상념으로서도 존재하지만, 실천으로 이어져야만 비로소 도덕으로서 역할을 할 수 있게 된다. 기본적으로 도덕이란 타자가 자신과 공존할 때, 그 타자와의 관계에서 좋음(goodness) 또는 옳음(righteousness)을 추구하는 것이기 때문이다. 그런 도덕이란 대부분 본능적으로 체득할 수 없기에 도덕교육이 있게 되는데, 한국인의 경우 서구와 다른 도덕적 전통을 갖고 있으며 따라서 서구적 도덕교육으로 충분하지 않거나, 전통적 도덕교육을 활용함으로써 더 나은 교육이 이루어질 수 있는 가능성을 찾을 수 있다. 그런 견지에서 전통의 실천적 의미를 재해석하고 활용하는 일은 한편의 논문으로는 너무 방대한 과제이다. 그래서 이 글에서는 비교와 범주화, 그리고 활용의 편의를 위해 전통을 ‘근세 유교(조선왕조 이후의 유교) 전통’으로 한정짓고, 다시 도덕교육 가운데 현행 도덕과 교육의 교육과정을 전통 교육과정과 비교함으로써 일정한 성과를 얻고자 하였다. 전통 교육과정은 기(氣)의 발달에 근거하여 연령별로 단계별 학습을 시도했으며, 그 핵심 이념은 인(仁)과 의(義), 실천적 덕목은 효(孝), 제(弟), 자(慈), 직(直), 근(勤), 검(儉)이라고 정리해볼 수 있다. 여기서는 그러한 전통 교육 이념이 ‘자신과의 관계’, ‘타인과의 관계’, ‘사회 공동체와의 관계’, ‘자연·초월과의 관계’로 영역을 나누고 각기 지향할 가치, 실천할 덕목을 배정한 현 도덕과 교육의 체계와 상통할 수 있다고 보고, 그에 따라 교육 현장에서 실천할 몇 가지 전통-현대 융합적 교육 방안들을 제안하였다. Any moral ideas get integrity upon their practices. Because in human society systems, morality have capability among human interactions, for it gives moral standards(good or bad/ right or wrong) to them. And when the society system is Korean, Koreans surely have developed unique styles of moral practice and moral education in their traditional ages. So reinterpretating and categorizing the moral tradition for taking advantage of it in contemporary context, make sense. In this study, Korean moral tradition is focused in its scope, as ``Confucian moral tradition in semi-modern era``. For convenience of study and the potentials of effective revision, Korean tradition have been resized. In Confucian moral tradition, semi-modern Korean moral ideas could be presented as ren(仁) and yi(義). Furthermore, ren and yi could be practiced with several moral virtues, like xiao(孝), di(弟), ci(慈), zhi(直) qin(勤) gian(儉). When compared with contemporary moral education idea systems, the traditional system can have affinity with the systems in moral ideas division(``with-self morality``, ``with-others morality``, ``with-universe and transcendence morality``). And several fusion-style, integration-based education practices can be developed and applied at contemporary school moral education.

Stain improvement in the white button mushroom ‘Seolgang’ and its varietal characteristics in Agaricus bisporus

Byung-Joo Lee,Mi-Ae Lee,Yong-Gyun Kim,Kwang-Won Lee,Yong-Pyo Lim,Byung-Eui Lee,Ho-Yeon Song 한국버섯학회 2012 한국버섯학회지 Vol.10 No.4

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, ‘Seolgang’ was developed by crossing two monokaryons ‘CM020913-27’ and ‘SSU423-31’. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of ‘Seolgang’ on CDA was better at 20℃ and 25℃ when it was compared with that of ‘505 Ho’. The mature cap shape of new strain ‘Seolgang’ is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain ‘505 Ho’, the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of ‘505 Ho’. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of ‘505 Ho’. Genetic analysis of the new strain ‘Seolgang’ showed different profiles compared to ‘505 Ho’, CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

Proteome Analysis of Nodule Development in both Sinpaldalkong2 and SS2-2

Lim,Chae-Woo,Park,Ji-Young,Hwang,Cheol-Ho 한국작물학회 2003 한국작물학회 학술발표대회 논문집 Vol.2003 No.-

한국어판 병동매일행동척도 개발

임호섭,신경철,함웅,채정호,김한오,정찬호 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.2

Objectives : Patient's behavior features are important factors which influences the clinical judgement including diagnosis. However, most psychiatrists build up a picture of patients' behavior from an amalgamation of their own brief observations and nurses' reports, which often lack in the objectiveness. Several behavioral scales have been developed to alleviate this difficulty, but the poor efficiency and reliability of these scales have made them less useful. The recently developed Ward Daily Behavior Scale is an objective tool for evaluating all the daily noteworthy behaviors of patients, and is easily applicable to wide ranges of diagnoses and ages. This study tried to prove the reliability and validity of the Ward Daily Behavior Scale-Korean version. Methods : The 112 patients, 63 males and 49 females, at a chronic psychiatric inpatient ward were selected as subjects. Experienced and unexperienced nurses rated patients' behaviors independently with the Ward Daily Behavior Scale-Korean version, after observing behaviors of subjects for 8 hours during day duty time. And then we tested the inter-rater reliability, internal consistency, and concurrent validity of this scale. Results : The Ward Daily Behavior Scale-Korean version proved to be both reliable and valid for measuring of behaviors of psychiatric inpatients. Conclusions : The Ward Daily Behavior Scale-Korean version will be a valuable tool to observe and quantify patients' behavior in psychiatric wards.

어린 브리티쉬 숏헤어 고양이에서 발생한 비대성심근병증

임수정,이선희,손선호,최호정,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

A l0-month-old, neutered male, British shorthair cat was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of tachypnea, anorexia and depression which onset were two days ago. On physical examination, increased respiratory rate and no murmur were found. A complete blood count indicated thrombocytosis. Results from a serum biochemical profile demonstrated elevations in CK, ALP, BUN and Ca, and decreases in sodium, potassium and chloride. Thoracic radiographs revealed patchy interstitial opacity increase and left atrium enlargement. Echocardiographs showed the evidences of hypertrophic cardiomyopathy which were hypertrophy of diastolic interventricular septum and left ventricular free wall. Other findings on echocardiography were the reverse E:A ratio(tranmitra1 inflow pattern) and absence of systolic anterior motion of mitral valve. Electrocardiographs demonstrated wide P and R wave, which mean left atrium enlargement and left bundle branch block, respectively. Medications including furosemide (2mg/kg), enalapril (0.25mg/kg), and spironolactone (1mg/kg) were prescribed twice a day and orally. Client education about exercise restriction and possibility of aggressive progress was given. Clinical signs suggestive of thromboembolism were developed on 6th day after showing tachypnea; right forelimb lameness, bilateral hindlimb lameness, loss of femoral pulse and severe pain. After all, euthanasia was performed at local animal hospital, according to the owner's request.

비부착 긴장재의 응력산정을 위한 극한강도 설계식의 평가

임재형(Lim, Jae-Hyung),문정호(Moon, Jeong-Ho),이리형(Lee, Li-Hyung) 한국콘크리트학회 1999 콘크리트학회논문집 Vol.11 No.3

사외이사 선임의 결정요인에 관한 연구: 사외이사 초과선임과 재임기간을 중심으로

이진호(Lee, Jin-Ho),임새훈(Lim, Sae-Hun) 한국경영교육학회 2020 경영교육연구 Vol.35 No.6

[연구목적] 본 연구에서는 법적 최소기준을 초과하여 사외이사를 선임한 기업과, 사외이사 재임기간을 3년 미만으로 관리하고 있는 기업의 특징을 살펴봄으로써 이를 중심으로 사외이사 제도를 개괄해 보고자 하였다. [연구방법] 본 연구는 2012년부터 2017년까지의 기간 동안 유가증권시장에 상장된 것으로 파악된 최종 3,861개의 기업표본을 대상으로 패널 고정효과 로짓모형(Panel Fixed Effects Logit Model)을 활용하여 분석을 실시하였다. [연구결과] 실증분석결과 기업규모가 클수록, 기업가치가 높을수록 사외이사를 초과 선임하고, 사외이사 재임기간이 길수록, 사내이사 규모가 클수록, 경영자 소유지분이 높을수록 사외이사를 초과 선임하지 않는 경향이 있는 것으로 나타났다. 또한 사내이사 및 사외이사 규모가 클수록, 잉여현금흐름 수준이 높을수록 사외이사 재임기간을 평균 3년 미만으로 관리하고, CEO 재임기간이 길수록, 기업규모가 클수록 사외이사 재임기간을 평균 3년 미만으로 관리하지 않는 경향이 존재하는 것으로 나타났다. [연구의 시사점] 본 연구의 결과는 국내 유가증권시장 상장기업들이 법적 최소기준을 충족하는 최소 규모의 이사회를 구성하는 방향으로 제도적 동형화 과정이 진행되어 왔고, 최고경영자가 경영자 안주가설을 지지하는 방향으로 사외이사 제도를 활용하고 있음을 시사한다. [Purpose] In this study, we tried to outline the outside directors system by examining the characteristics of firms that appointed outside directors in excess of the legal minimum standards and that the outside directors tenure is less than 3 years. [Methodology] This study tested the final 3,861 firm-year samples found to be listed on the KOSPI market during the period from 2012 to 2017 using a Panel Fixed Effects Logit Model. [Findings] Following our empirical results, the larger the firm size, the higher the Tobin’s Q, the more the outside directors were appointed, whereas the longer the outside directors tenure, the larger the size of inside directors, and the higher the CEO ownership, the more the outside directors tended not to be appointed. In addition, the larger the size of inside and outside directors, the higher the free cash flow level, the outside directors tenure is managed to be less than 3 years, whereas the longer the CEO tenure and the larger the firm size, the more likely there is a tendency not to manage the outside directors tenure for less than 3 years. [Implications] The empirical results suggests that the institutional isomorphization process has been conducted in the direction of forming the smallest boards that meet the legal minimum standards for firms listed in the KOSPI markets, and the CEO is using the outside directors system in a way to support the Management Entrenchment Hypothesis.