Breeding of Tetraploid in Codonopsis lanceolata (Sieb. et Zucc.) Trautvetter by Colchicine Treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Codonopsis lanceolata. Three tetraploid plants of C. lanceolata were produced from seeds which absorbed 0.1 % colchicine solution for 12 hours, and 0.5% colchicine solution for 1 and 6 hours from seedlings, respectively. But tetraploid was not produced from shoot tips treated by colchicine solution. Compared to diploid, tetraploid plants had larger stomata, but less number of stomata. Fresh weight of tetraploid plants was 1.4∼3.6 times heavier than diploid plants.

Breeding of Tetraploid in Platycodon grandiflorum (Jacq.)A. DC. by Colchicine treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Platycodon grandiflorum in Campanulaceae. The most successful colchicine treatment for tetraploid production in P. grandiflorum was soaking treatment using 0.01 and 0.5% colchicine solution for 1 hour and 12 hours, respectively. Morphological characteristics of both diploid and tetraploid were similar, but tetraploid plants had more leaves. Compared to diploid, tetraploid had the larger stomata, but less number of stomata. Fresh weight of tetraploids was 20∼40% heavier than that of diploid.

전립선암의 예측인자로서 전환대 전립선특이항원의 유용성

김현학,권동득,오봉렬,박광성,류수방,박양일 全南大醫大 2001 전남의대학술지 Vol.37 No.3

여자 유도선수들의 트레이닝과 시합시즌에 따른 혈구세포, 헤마토크리트 및 헤모글로빈 농도의 특성

김학렬,조현철 龍仁大學校 1998 용인대학교 논문집 Vol.15 No.-

The main objective of this study was investigated a "Sports Anemia" phenomenon as estimation a profilex of hematological status during training and competition season. Eleven elite women judoist was recruited to participate as subject of this study. The experimental periods was done lasting for one years and began on March and ended on October. that is, it was done a 1st measurement on March, 2nd measurement on June, 3rd measurement on September. After 3rd measurement all athletes was performed to judo competition and then, it was done a 4rd measurement on October after resting a 4 weeks periods. Values for all hematological parameters are within the normal range for elite women judoist. White Blood Cell (WBC). There was a significant difference between the WBC count of March(1st measurement) and June(2nd measurement), September(3rd measurement), and October(4rd measurement) (F-value=6.077, p〈.01). Red Blood Cell (RBC). There was a significant difference between the RBC count of March vs. June, September and October (F-value = 7.857 p〈.001). Hematocrit (Hct). There was no significant difference throughout training and competition season (F-value=1.233). Hemoglobin (Hb). There was no significant difference between the hemoglobin concentration of training and competition season (F-value= 1.962). Mean Corpuscular Volume (MCV). There was a significant difference between the MCV values of March vs. June, September and October, Also, between the MCV values of June vs. October and September vs. October (F-value =44.638, p〈.001). Mean Cell Hemoglobin (MCH). There was no significant difference between training and season, except March vs. June (F-value =4.137, p〈.05). Mean Cellular Hemoglobin Content (MCHC). There was a significant difference between the MCHC of March vs. June, September and October (F-value = 28.609, p〈.001). Our results suggest that Mean values for hematological parameters following training and competition season in elite women judoist are remained within the normal range, and that "Sport Anemia" cannot observed a throughout training and competition season.

지베렐린과 에세폰의 葉面撒布가 '美白' 복숭아의 熟期와 品質에 미치는 影響

김영호,윤철구,임상철,김학현,이철희,최관순,김선규 충북대학교 농업과학기술연구소 1999 農業科學硏究 Vol.16 No.-

In order to obtain the basic data for artificial maturity control of 'Mibaek' peach, GA and ethephon were foliar sprayed 4 weeks prior to harvest, and their effects on fruit maturity and quality were examined. GA and ethephon tended to decrease the fruit weight except 100mg ㆍ L-1 GA+50 to 100mg · L-1 ethephon treatment. GA increased the fruit firmness, and ethephon at higher concentration increased the fruit drop. Harvest date was advanced for 7 to 8 days by ethephon while was delayed for 6 to 8 days by GA treatment, regardless of concentration.

성장에 따른 백서 절치유두의 신경 분포 변화

김영진,남순현,김현정,이재문,구대학 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

출생후 성장에 따른 절치 유두의 신경분포 및 변화를 관찰하기 위하여 생후 1, 5, 10, 15, 20, 25, 35일 된 백서와 완전히 성장한 백서를 대상으로 calcitonin gene related peptide (CGRP)를 이용한 면역조직 화학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. 생후 1일군에서는 염주알 모양의 굵은 신경섬유가 성긴 결합조직에 나타났다. 5일군에서는 이러한 신경섬유의 밀도와 분지가 증가하여 상피하층으로 더욱 가까이 연장되었고 여러개의 varicosities도 관찰되었다. 10일군부터 신경섬유의 분지 및 밀도가 증가하였고 상피하층에서 신경총을 관찰할 수 있었으며 상피내 신경섬유의 진입을 관찰할 수 있었다. 20일군에서는 증가된 상피층내 신경섬유를 관찰할 수 있었으나 25일군에서부터 전반적인 신경섬유의 분포 및 밀도가 많이 감소되었으며 성장이 완료된 군에서는 chemosensory corpuscle 지역을 제외하고는 신경섬유를 거의 관찰할 수 없었다. The purpose of this study was to investigate the distribution of nerves in the incisive papilla of rat by each developing stage. The distribution on nerves in the incisive papilla was investigated by means of immunohistochemistry using an antibody against calcitonin gene related peptide (CGRP). The results were as follows : At 1 day after birth, CGRP-immunoreactive(IR) nerve fibers first appeared in loose connective tissue without forming an apparent terminal structure. At 5 days, the CGRP-IR nerve fibers increased in density and extended more to subepithelium. CGRP-IR nerve fiber began to penetrate into the epithelium at 10 days and reached maximum at 20 days. At 25 days, intraepithelial nerve fibers were not found, and the distribution and density of nerve fiber began to decrease. In adult rat, the nerve fiber almost could not be found in dome region except in chemosensory corpuscle region.

흰쥐의 연령증가에 따른 일시적 최대운동 부하시 혈장 콜레스테롤, HDL, LDL 및 TG 농도의 변화

김학렬,조현철,이선장,김영란,최용어 龍仁大學校 體育科學硏究所 1993 體育科學硏究論叢 Vol.3 No.1

The purpose of this study estimated whether a life style and diet pattern or natural phenomenon induced to aging in changes of plasma lipid component. Also, It was estimated from the changes of plasma lipid component followed acute maximal swimming exercise in different aged rats. Three months, nine months and sixteen months male rats of a Sprague-Dawley were housed in individual cages, and they were separated into each two group. - 3 month (12) : Control (6) and Experimental (6) - 9 month (22) : Control (10) and Experimental (12) - 16 month(16) : Control (8) and Experimental (8) In the 3.9.16 month, half of control group was randomly sacrificed. The other half of experimental group was performed with swimming exercise until exhaustive state. Water tank of 50cm depth and 50cm diameter was used for this study. Rats were given a 5 min warm up with swimming exercise, this warm up was followed by taking 5 min rest period, then animal exercised until exhaustion. Plasma cholesterol, HDL, LDL and TG levels followed aning of 3,9 and 16 months was not shown a significant difference between control group. Also, Plasma cholesterol, HDL and LDL levels of experimental group was not displayed statistically significant difference between 3,9 and 16 month group, but there was a significant difference(p<.05) between control and experimental group of 16 month, and between experimental group of 3 month and 16 month(p<.05) in levels of triglycerides. Conclusively, This study suggested that changes of plasma lipid component induced by aging was not phenomenan due to increase of age, and conditions except for life style and diet patern, etc. was similar to a changes of lipid component induced as exercise.

강력한 운동부하후 혈중 젖산농도에 대한 성차의 비교

김학렬,조현철,최용준,김의영,안병근 龍仁大學校 體育科學硏究所 1995 體育科學硏究論叢 Vol.5 No.1

The purpose of this study was to compare sex differences of blood lactate concentration after strenous 800m sprint running and recovery 15 min. Trained-middle distance runners(Male:11, Female:10) participated as subjects of this paper. All subjects was performed a 800m sprint running, repeatedly. Blood samples for analysis a blood lactate concentration was collected in rest, warm-up after, lst 800m run after, recovery 15 min and 2nd 800m run after, respectively. The results and conclusion of this study was obtained as follows : 1. Male 800m best record in lst and 2nd run was shown a significantly high record, compared to female(lst: p<0.01, 2nd: p<0.001). 2. Blood lactate concentration after lst and 2nd run was not significant difference between male and female. However, Blood lactate concentration of both group displayed more high levels in 2nd run than lst run(p<0.001, respectively). 3. Blood lactate concentration of recovery phase was shown a significantly high lactate removal ability in male, compared with female(p<0.001). As results of above, Peak blood lactate concentration of male and female was not significant difference, but, male was shown a faster lactate removal ability compared with female.

투기종목 선수들의 무산소성 역치의 특성

조현철,송순천,김춘경,김의영,안용규,이규석,김학렬 龍仁大學校 體育科學硏究所 1991 體育科學硏究論叢 Vol.1 No.1

The purpose of the present study is to determine the levels of maximal oxygen uptake and anaerobic threshold of boxing players(n=23), ken-do players(n=18), Judo players(n=12), T.D.K players(n=18). Treadmill test was used to measure maximal state and anaerobic threshold of metabolic variables. Treadmill exercises protocol was 2-min incremental test from an initial work load of 0MPH(grade : 0%) to all-out by step of 1.0MPH(grade : 2%). The results of measurement was as follows : 1. Absolute(1/min) and relative(ml/kg/min) maximal oxygen uptake was highest(5.08l/min, 65.01 ml/kg/min) in Judo layers, followed in order by the T.D.K players (4.37l/min, 64.74 ml/kg/min), Boxing players(4.18) l/min, 63.19 ml/kg/min) and ken-do players(3.81l/min, 58.65 ml/kg/min). It is not significant difference in the relative maximal oxygen uptake, but is significant difference between group in the absolute maximal oxygen uptake(p<0.001) 2. AT-VO₂(l/min) and AT-VO₂(ml/kg/min) in the anaerobic threshold was highest (3.71 l/min, 47.28 ml/kg/min) in Judo players, followed in order by the T.K.D players(3.29 l/min, 48.75 ml/kg/min), boxing players(2.90 l/min, 43.91 ml/kg/min) and ken-do players(2.82 l/min, 43.52 ml/kg/min). It is not significant difference in the AT-V02(ml/kg/min), but is significant difference between group in the AT-V02(l/min). (p<0.001) 3. %HRmax and %V 02max in the anaerobic threshold was not significant difference between group.

경기종목의 특성에 따른 유·무산소성 대사능력 및 무산소성 파워에 관한 실험적 연구

조현철,송순천,김학렬,손태열 成均館大學校 科學技術硏究所 1989 論文集 Vol.40 No.2

This experimental study was designed, 1) to observe maximal oxygen uptake and anaerobic threshold during incremental loading treadmill exercise by 2-min interval. 2) to determine the anaerobic power in the wingate test during bicycle ergometer work with toe-stirrups. The subject were 16 college elite boxing player, 10 college elite soccer player The results were as -follows: 1. The means±SD of maximal oxygen uptake and maximal oxygen uptake per kg body weight were 4.06±0.66l/min, 4.27±0.46l/min, and 60.58±7.61ml/kg/min 63.05±3.41ml/kg/min, respectively. also maximal heart rate were 187.88±9.44 beats/min, 185.40±6.57 beats/min. respectively. 2. The means±SD of maximal oxygen uptake and maximal oxygen uptake per kg body weight at anaerobic threshold was 2.93±0.5l/min, 3.23±0.47l/min (AT-VO2. l/min), and 43.70±6.97ml/kg/min, 47.71±4.99ml/kg/min (AT-VO2. ml/kg/min), respectively. also Anaerobic Treshold level (AT-% VO2max) was about 72.49±4.37% VO2max, 75.37±8.8% VO2max, and AT-% HRmax was about 86.07±5.97% HRmax, 87.84±6.25% HRmax, respectively. 3. The means ±SD of Anaerobic Power in the wingate test was 592.50±65.42 Wattes, 588.00±46.30 Wattes, respectively. The results of this study indicated that boxing players are incresed training program and exercise prescription for anaerobic metabolic process, soccer players are done training program for aerobic metabolic process, because of decresed maximal oxygen uptake and anaerobic threshold level.