Sargassum horneri ethanol extract improves the type-1 allergic responses in IgE-activated mast cells and house dust mite (HDM)/2,4-dinitrochlorobenzene (DNCB)-stimulated NC/Nga mice model

Ginnae Ahn(안긴내),Eui Jeong Han,Min Ju Kim,Disanayaka Mudiyanselage Dines,Mawalle Kankanamge Hasitha Mad,Ilekuttige Priyan Shanura Fern,Hyun-Soo Kim,Seo-Young Kim,You-Jin Jeon,Youngheun Jee 한국수산과학회 양식분과 2020 한국수산과학회 양식분과 학술대회 Vol.2020 No.7

방사선을 조사한 마우스에서 비장세포에 대한 톳의 보호 작용

김아름,빙소진,조진희,안긴내,이지혁,전유진,이병걸,지영흔,Kim, Areum,Bing, So Jin,Cho, Jinhee,Ahn, Ginnae,Lee, Ji-Hyeok,Jeon, You-Jin,Lee, Byung-Gul,Jee, Youngheun 대한수의학회 2015 大韓獸醫學會誌 Vol.55 No.1

The immune system is specifically sensitive to oxidative stress induced by ionizing radiation because of its rapid proliferative activity. For this reason, an instructive immune system is one of the best ways to minimize side effects, such immunodeficiency, of gamma radiation. Over the past few decades, several natural plants with antioxidant and immunomodulatory properties have been identified as adjuncts for nontoxic and successful radiotherapy. Hizikia fusiforme extract (HFE) containing plentiful dietary fiber and fucoidan is known for its instructive antioxidant capacity, immunomodulation abilities, and immune activation. In this study, we determined whether HFE protects radiosensitive immune cells from gamma radiation-induced damage. C57BL/6 mice were irradiated with gamma-ray. The effect of HFE on the ionizing radiation damage of immune cells was then evaluated with an MTT assay, 3H-thymidine incorporation assay, and PI staining. We found that HFE stimulated the proliferation of gamma-ray irradiated immune cells without cytotoxic effects. We also observed that HFE not only decreased DNA damage but also reduced gamma radiation-induced apoptosis of the immune cells. Our results suggest that HFE can protect immune cells from gamma-ray damage and may serve as an effective, non-toxic radioprotective agent.

C57BL/6 마우스에서 Retroviral 벡터를 이용한 Foxp3 유전자의 도입에 의한 Foxp3 단백의 발현 양상

황인선,하단비,빙소진,전경익,안긴내,김대승,조진희,임재학,임신혁,황규계,지영흔,Hwang, Insun,Ha, Danbee,Bing, So Jin,Jeon, Kyong-Leek,Ahn, Ginnae,Kim, Dae Seung,Cho, Jinhee,Lim, Jaehak,Im, Sin-Hyeog,Hwang, Kyu-Kye,Jee, Youngheun 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.3

The maintenance of peripheral immune tolerance and prevention of chronic inflammation and autoimmune disease require $CD4^{+}CD25^{+}$ T cells (regulatory T cells). The transcription factor Foxp3 is essential for the development of functional, regulatory T cells, which plays a prominent role in self-tolerance. Retroviral vectors can confer high level of gene transfer and transgene expression in a variety of cell types. Here we observed that following retroviral vector-mediated gene transfer of Foxp3, transductional Foxp3 expression was increased in the liver, lung, brain, heart, muscle, spinal cord, kidney and spleen. One day after vector administration, high levels of transgene and gene expression were observed in liver and lung. At 2 days after injection, transductional Foxp3 expression level was increased in brain, heart, muscle and spinal cord, but kidney and spleen exhibited a consistent low level. This finding was inconsistent with the increase in both $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell frequencies observed in peripheral immune cells by fluorescence-activated cell-sorting (FACS) analysis. Retroviral vector-mediated gene transfer of Foxp3 did not lead to increased numbers of $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell. These results demonstrate the level and duration of transductional Foxp3 gene expression in various tissues. A better understanding of Foxp3 regulation can be useful in dissecting the cause of regulatory T cells dysfunction in several autoimmune diseases and raise the possibility of enhancing suppressive functions of regulatory T cells for therapeutic purposes.

자가면역성 뇌척수염을 유도한 C57BL/6 마우스 큰포식세포에서의 Galectin-3의 과발현

김대승,황인선,박석재,안긴내,박상준,박현정,주홍구,지영흔,Kim, Dae Seung,Hwang, Insun,Park, Suk-jae,Ahn, Ginnae,Park, Sang-Joon,Park, Hyun Jeong,Joo, Hong-Gu,Jee, Youngheun 대한수의학회 2011 大韓獸醫學會誌 Vol.51 No.2

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease in the murine central nervous system (CNS) and has long been used as an animal model for human multiple sclerosis. Development of EAE requires coordinated expression of a number of genes that are involved in the activation and effector functions of inflammatory cells. Galectin-3 (Gal-3) is a member of the betagalactoside- binding lectin family and plays an important role in inflammatory responses through its functions on cell activation, cell migration or inhibition of apoptosis. We investigated the functional role of Gal-3 in EAE mice following immunization with myelin oligodendrocyte glycoprotein $(MOG)_{35-55}$ peptide. During the peak stage of EAE, the localization of Gal-3 in inflammatory cells markedly increased in subarachnoid membranes and perivascular regions of CNS. In contrast, Gal-3 was weakly detected in cerebrum and spinal of the recovery stage of EAE. Consistent with this finding, western blot analysis revealed that Gal-3 expression was significantly increased at the peak stage while it was slightly decreased at the recovery stage in the CNS. In addition, the population of $CD11b^{+}$ macrophage expressing Gal- 3 in spleen of EAE mice was markedly increased compared with control mice. In fact, most of activated macrophages isolated from spleen of EAE mice expressed Gal-3. Taken together, our results demonstrate that the over-expression of Gal-3 in activated macrophages may play a key role in promoting inflammatory cells in the CNS during EAE.

간세포에서 hydrogen peroxide 처리가 유도한 산화적 손상에 대한 참소라 육 가수분해물의 보호 효능 평가

한의정 ( Eui Jeong Han ),안긴내 ( Ginnae Ahn ),신은지 ( Eun-ji Shin ),이영주 ( Young-ju Lee ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.3

본 연구에서는 참소라 육으로부터 제조된 Alcalase 효소 가수분해물(BMA)의 항산화 효과와 간보호 효능 및 관련 기전을 규명하였다. BMA는 풍부한 타우린을 포함하고 있으며, H₂O₂가 처리된 hepatocytes에서 세포독성 없이 효과적으로 세포 내 ROS 생성량을 감소시킴으로써 효과적으로 세포를 보호하였다. 뿐만 아니라, BMA는 apoptosis 관련 인자의 발현량을 조절하고 MAPK과 NFκB 신호전달경로의 활성화를 효과적으로 억제함으로써 H₂O₂ 처리에 의해 야기된 산화적 손상을 억제시켜 세포를 보호한다는 것을 확인할 수 있었다. 이상의 결과로부터, BMA는 산화적 손상에 의해 야기되는 간질환에 있어 부작용이 없는 천연 소재로서의 잠재적인 자원이 될 수 있음을 시사한다. In this study, we investigated the protective effect of an Alcalase enzymatic hydrolysate derived from Batillus cornutus meat (BMA) against hydrogen peroxide (H₂O₂)-induced oxidative damages in hepatocytes. First, we confirmed that BMA containing plentiful taurine has the antioxidant activities by scavenging 2,2-azino-bis (3-ethylbenzthiazoline)- 6-sulfonic acid radical (ABTS ⁺ ) and increasing the reducing power and oxygen radical absorbance capacity (ORAC) value. Also, the treatment of BMA increased the cell viability by reducing the intracellular reactive oxygen species (ROS) production in H₂O₂-stimulated hepatocytes. In addition, BMA effectively decreased the sub-G₁ DNA contents and the apoptotic body formation increased by H₂O₂ stimulation via modulating the protein expression levels of apoptotic molecules (Bcl-2, Bax and p53). Moreover, BMA suppressed the activation of extracellular signal-regulated kinase (ERK)/p38 and nuclear factor (NF)κB signaling by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling known as antioxidant systems in H₂O₂-stimulated hepatocytes. These results suggest BMA protects hepatocytes against oxidative damages caused by H₂O₂ stimulation.

유해 녹조에 대한 황토 및 점토와 키토산 혼합물의 응집 및 살조 효과

이현수 ( Hyun-su Lee ),정경원 ( Gyeong-won Jeong ),최창용 ( Changyong Choi ),안긴내 ( Ginnae Ahn ),나재운 ( Jae-woon Nah ) 한국키틴키토산학회 2017 한국키틴키토산학회지 Vol.22 No.1

Currently, harmful green-tide is seriously occurred by water temperature rising by climate change and inflow of excessive nutritive salts in river. To remove these harmful green-tide, red-soil and clay are using in river. However, it is induced to high cost, damage of forestland, and destruction of ecosystem. In addition, only red-soil and clay are sunken to harmful green-tide, which is growth in bottom of river. It is cause of secondary pollution. To solve these problem, we prepared to red-soil/clay-mixed chitosan. In this study, characterization of chitosan was analyzed by ¹H-NMR and GPC-MALLS. Also, to confirm optimal concentration of flocculation, red-soil or clay of various concentration with chitosan of fixed concentration (5 mg/L) was treated in harmful green-tide. Moreover, its algicidal effect was analyzed by UV-vis spectrometer and, growth and chlorophyll a of harmful green-tide were decreased by red-soil/clay-mixed chitosan. Theses results suggest that red-soil/clay-mixed chitosan can be inhibit against harmful green-tide.

Loperamide로 유도된 변비 마우스 모델에서 참모자반(Sargassum fulvellum)의 장 기능 개선 효능 평가

한의정 ( Eui Jeong Han ),김민주 ( Min Ju Kim ),신은지 ( Eun-ji Shin ),안긴내 ( Ginnae Ahn ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.1

In this study, we investigated laxative effects of Sargassum fulvellum (S. fulvellum, SF) on loperamide-induced constipation in mice. First, the hot water extracts of SF (SFH) showed higher carbohydrate contents (26.65 ± 0.53%) than protein (9.41 ± 0.31%) and phenol (2.82 ± 0.00%). Oral administration of SFH effectively increased the body weight and the food intake decreased by loperamide intake in mice. Also, SFH improved the intestinal function by significantly increasing the number, weight and moisture contents of fecal pellets in loperamide-treated mice. Taken together, these results indicated that SFH has laxative effects on loperamide-induced constipation in mice and it might be used as a natural food material for improving or preventing constipation.

피부각질형성세포에서 자외선 B 조사가 야기한 염증반응에 대한 괭생이모자반 초음파 에탄올 추출물의 억제 효능 평가

한의정 ( Eui Jeong Han ),고창익 ( Chang-ik Ko ),안용석 ( Yong-seok Ahn ),안긴내 ( Ginnae Ahn ) 한국키틴키토산학회 2022 한국키틴키토산학회지 Vol.27 No.4

본 연구에서는 UVB 조사에 의해 야기된 HaCaT 세포 내 염증 반응에 대한 괭생이모자반 초음파 에탄올 추출물(USHE)의 억제 효능에 대한 연구를 수행하였다. USHE는 세포독성 없이 UVB 조사에 의해 증가된 세포 내 NO 생성량을 감소시킴으로써, 세포 생존율을 유의적으로 향상시켰다. 또한, USHE 처리는 MAPKs 및 NFκB 신호전달경로의 활성화를 억제함으로써 UVB 조사가 야기한 세포 내 cytokines 및 chemokine의 생성량을 감소시켰다. 뿐만 아니라, USHE는 UVB 조사에 의해 증가된 염증 관련 매개인자인 iNOS 및 COX-2의 발현량을 감소시켰다. 모든 결과로부터 본 연구는 USHE가 UVB 조사에 의해 야기된 염증 반응을 효과적으로 억제시켜 HaCaT 세포를 보호한다는 것을 확인할 수 있었고 부작용을 가지지 않은 천연소재로서 이용가치가 매우 높다는 것을 제시한다. The purpose of this study was to investigate the inhibitory effects of a ultrasonic ethanol extract from Sargassum horneri (USHE) against ultraviolet B (UVB)-induced skin inflammation in HaCaT cells, a human keratinocyte. USHE markedly increased the cell viability by reducing the generation of nitric oxide (NO) in UVB-exposed HaCaT cells. Also, USHE effectively down-regulated the expression levels of iNOS and COX-2 known as inflammatory mediators. Additionally, USHE decreased the production of inflammatory cytokines (interleukin (IL)-6, 8, tumor necrosis factor (TNF)-α and thymic stromal lymphopoietin (TSLP)) and chemokines (macrophage-derived chemokine (MDC) and thymus and activation-regulated chemokine (TARC)) by suppressing the activation of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)κB signaling in UVB-exposed HaCaT cells. Taken together, these results suggest that USHE inhibited the inflammatory response in UVB-exposed HaCaT cells.

산화적 손상에 대한 키조개(Atrina pectinata) 효소 가수분해물의 간세포 보호 효과

한의정 ( Eui Jeong Han ),신은지 ( Eun-ji Shin ),김기웅 ( Kee-woong Kim ),안긴내 ( Ginnae Ahn ),배태진 ( Tae Jin Bae ) 한국수산과학회(구 한국수산학회) 2020 한국수산과학회지 Vol.53 No.1

In this study, we investigated the protective effects of a Neutrase enzymatic hydrolysate derived from Korea pen shell Atrina pectinata (APN) against hydrogen peroxide (H₂O₂)-induced oxidative damage in hepatocytes. First, we confirmed that APN has antioxidant activities by scavenging 2,2-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS⁺) and H₂O₂ and increasing oxygen radical absorbance capacity (ORAC) value. Also, the treatment of APN increased the cell viability by reducing the intracellular reactive oxygen species (ROS) production in H₂O₂-stimulated hepatocytes. In addition, APN decreased the sub-G₁ DNA contents and the apoptotic body formation increased by H₂O₂ stimulation. Moreover, APN modulated the protein expression of apoptosis related molecules (Bcl-2, Bax and p53) by suppressing the activation of nuclear factor NFkB and ERK/p38 signaling in H₂O₂-stimulated hepatocytes. Furthermore, APN led to the activation of Nrf2/HO-1signaling known as antioxidant systems. These results suggest APN protects hepatocytes against oxidative damages caused by H₂O₂ stimulation.

큰잎모자반 효소적 추출물의 항산화 활성

고석천(Seok-Chun Ko),강성명(Sung-Myung Kang),안긴내(Ginnae Ahn),양현필(Hyun-Pil Yang),김길남(Kil-Nam Kim),전유진(You-Jin Jeon) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.4

본 연구에서는 제주 연안에 서식하는 갈조류 중 큰잎모자반의 항산화활성을 확인하기 위하여 큰잎모자반으로부터 효소적 가수분해를 이용하여 추출물을 제조한 후 이들로부터 활성산소종의 소거활성을 확인하였다. 여기서 효소적 가수분해를 이용하여 제조된 큰잎모자반의 효소적 추출물은 항산화활성이 우수하였으며, 효소적 추출물간의 유의적 차이는 없었다. 또한 단백질 분해효소 추출물에서는 Neutrase 추출물이 항산화 활성이 우수하였으며, 당 분해효소 추출물에서는 Celluclast를 이용하여 제조된 효소적 추출물이 다소 우수한 항산화효과를 나타내었다. 이와 같은 결과는 여러 가지 효소들이 복합되어진 효소가 세포벽에 있는 섬유질이나 당단백질 혹은 알긴산 고분자물질 등을 분해시키는 작용을 유도하였기 때문이라고 사료된다. 따라서 수용성의 추출물은 유기용매 추출물의 단점을 보완할 수 있을 뿐만 아니라 큰잎모자반 효소적 추출물의 생리활성물질은 식품을 포함한 다양한 분야에 응용시킬 수 있을 것으로 사료되며, 이상의 결과로 큰잎모자반은 잠재적인 기능성식품 소재로서의 가능이 충분하다고 판단된다. In this study, Sargassum coreanum was enzymatically hydrolyzed to prepare water-soluble extracts by using five carbohydrates (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavozyme and Alcalase) and their potential antioxidant activity were evaluated. The Celluclast and Neutrase extracts of Sargassum coreanum exhibited better DPPH radical scavenging activities (92.42% and 92.78%, respectively) and hydrogen peroxide (H₂O₂) scavenging activities (58.28% and 57.97%, respectively) compared to those of other enzymatic extracts. These results suggest that Sargassum coreanum would be a good raw materials for antioxidant and enzymatic hydrolysis would be a good strategy to prepare antioxidant extracts from seaweeds.