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Ilekuttige Priyan Shanura FERN,Eui Jeong HAN,Min Ju KIM,Ginnae AHN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4
Recent studies demonstrate that the dysregulated production of reactive oxygen species (ROS) and the resulting oxidative stress are implicated in the pathogenesis of numerous detrimental cellular responses caused by numerous factors including, xenobiotics, exposure to ionizing radiation, and disease conditions. At present, a special interest is paid towards research on antioxidants for the prevention of oxidative stress and associated health issues. Moringa oleifera is a well-known source of biocompatible antioxidants. The present study discloses the evaluation of the antioxidant activity of M. oleifera hot water extract (MOH) on H2O2 induced oxidative stress in Vero cells. Per analysis, MOH indicated DPPH and ABTS<sup>+</sup> radical scavenging with IC values of 102.52 and 122.55 µg/ml. MOH dose-dependently reduced intracellular ROS generation in H2O2 stimulated Vero cells while increasing the cell viability with the best bioactivity at 125 µg/ml. Further analysis using fluorescence microscopy and flowcytometry supported the above findings. H2O2-induced mitochondrial depolarization and apoptosis were dose-dependently suppressed by MOH. The suppression of apoptosis was mediated via the mitochondrial apoptosis pathway. Further analysis of MOH bioactivity and safety would potentiate its applications in manufacturing functional food.
Ilekuttige Priyan Shanura Fern,Eui Jeong Han,Kirinde Gedara Isuru Sandanuwa,Hyeong Nam Jeon,Seo-Young Park,Arachchige Maheshika Kumari Ja,Ginnae Ahn 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Studies on the impact of Fine dust (FD) on the skin as a causal factor of chronic skin disorders including skin barrier abnormalities, pruritus, and immunological dysregulations have recently received an increased interest. Effects of FD collected from Beijing, China on HaCaT keratinocytes were herein investigated. Based on dose and time response analysis, FD treatment-induced intracellular ROS generation and caused cell damage. Intracellular ROS were evaluated by fluorescence microscopic and FACS analysis. FD exposure-induced mitochondrial depolarization and apoptosis as evidenced by apoptotic body formation, and accumulation of sub-G1 hypodiploid cells. The events proceeded via mitochondria and ER stress-mediated apoptosis pathways. Further, FD elevated inflammatory responses as elicited by the activation of key mediators in the NF-κB pathway. Moreover, FD disrupted skin barrier functions involved in the formation of tight junctions and moisturization. This work has supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2020R1I1A1A01073203).
Hyeong Nam Jeon,Ilekuttige Priyan Shanura Fern,Seo-Yeong Park,Kirinde Gedara Isuru Sandanuwa,Arachchige Maheshika Kumari Ja,Eui Jeong Han,Ginnae Ahn 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The present study evaluated the effect of a low molecular weight fucoidan obtained from a celluclast extract of Sargassum siliquastrum (S. siliquastrum) against improvement of skin inflammation and moisturizing effect caused by ultraviolet B (UVB) exposure in HaCaT cells. First of all, we prepared the four kinds of polysaccharides (SSS1, SSS2, SSS3 and SSS4) from the celluclast extract of S. siliquastrum by a step gradient ethanol precipitation. Among them, SSS4 consisted of the higher sulfate content showed the higher antioxidant activities. Also, UVB protective effect confirmed that SSS4 reduced intracellular ROS production while improving cell viability. In addition, SSS4 inhibited the activation of MAPK and NFκB signaling in UVB-exposed HaCaT cells. Moreover, SSS4 significantly increased the amount of hyaluronic acid by modulating the expression levels of skin barrier-related proteins and moisturizing factors. This study suggested that SSS4 is a fucoidan and has potential therapeutic effects on skin damage caused by UVB exposure in HaCaT cells. This work (Grants No. 2020R1A6A3A 0109950011) was supported by National Research Foundation of Korea in 2021.
Seo-Yeong Park,Eui Jeong Han,Ilekuttige Priyan Shanura Fern,Hyeong Nam Jeon,Kirinde Gedara Isuru Sandanuwa,Arachchige Maheshika Kumari Ja,Hyun-Soo Kim,Ginnae Ahn 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study investigated the effect of phlorofucofuroeckol A (PeA) isolated from Ecklonia cava against inflammatory response in the tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ) stimulated keratinocytes and a TPA-stimulated ear edema mice model. The result showed PeA reduced the generation of intracellular reactive oxygen species (ROS) without the cytotoxicity at the used all concentrations in TNF-α/IFN-γ -stimulated keratinocytes. Also, PeA significantly down-regulated the mRNA expression and the secretion of inflammatory cytokines and chemokines. In addition, PeA effectively inhibited the formation of apoptotic body and the population of sub-G1 DNA content by suppressing the activation of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB signaling. Moreover, PeA activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) signaling in TNF-α/IFN-γ-stimulated keratinocytes. In further study, PeA inhibited the ear edema by reducing the expressions of inflammatory mediators in TPA-stimulated mice model. This work (Grants No. M01201820150306) was supported by KIMST.