Enhanced Systemic siRNA Delivery Using Reducible Bile Acid-modified PEI for Cancer Therapy

Chae eun PARK,Su yeon LIM,Da hwun KIM,Siyan LYU,Byung deok KIM,Oui bo WHANG,Ji hoon JEONG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Small interfering RNA (siRNA) contributes to RNA interference(RNAi) by inhibiting target gene expression in a sequence-specific manner. siRNA delivery system have become increasingly popular for cancer therapy. In this study, we used a low molecular weight polyethylenimine (PEI, 1.8 kDa) conjugated with deoxycholic acid (DA). A DA-PEI conjugate was further modified with 4-fluorothiophenol (4-FTP) (4-FTP-DA-PEI) to enhance systemic siRNA delivery. The thiophenol group would be involved with disulfide bonds between the polymer chains and siRNA modified with free thiols (thiol-siRNA) to form and π-π interactions between the pendent aromatic groups and coprostane ring of the bile acid. The stabilized TP-DA-PEI conjugate with thiol-siRNA achieved developed intracellular uptake, serum stability, and transfection efficiency. Furthermore, it shows high accumulation of TP-DA-PEI/thiol-siRNA polyplexes and significant tumor growth inhibition effect in tumor-bearing mice after systemic administration.

노인과 대학생의 낙상 위험 분석

정다현,김예희,김지은,이예영,강현주 순천향대학교 기초과학연구소 2021 순천향자연과학연구 논문집 Vol.27 No.1.2

The purpose of this study was to analyze risk of falls between elderly people and college students. The subjects of this study were 25 people aged 70 years or older and 17 college students located in Asan. We measured sensory, integrated balance, nervous, musculoskeletal system and HQR by FRA system (Inbody, Korea). The results of measurement showed significant differences in sensory system (p<.01), integrated balance (p<.001), nervous system (p<.001), and musculoskeletal system (p<.001) between groups. These results suggest that aging makes difference of function of these systems. In our view, counterplans need to be implemented, including exercise intervention to prevent decreases of fall risk,

브랜딩·스타일링·마케팅을 결합한 스페이스 디자인 프로세스 연구 - 작품‘Fill the Red, Feel the Red'를 중심으로 -

은다솜,장효실,최해원,홍지현,이진민,장미정,이성애 숙명여자대학교 디자인연구소 2018 숙명디자인학 연구 Vol.25 No.-

본 연구는 디자인 분야와 마케팅 분야의 전략을 종합하여 전략적 연결점을 찾아내 요소들 간 융합을 이루는 사례를 제시하여 브랜드·스페이스·마케팅에서 새로운 방향성과 아이디어를 제시하는데 목적을 둔다. 이에 본 연구는 2017년 9월~12월까지 ㈜애경과 산업 연계하여 진행된 숙명여대 환경디자인학과 ‘브 랜딩·스타일링·마케팅’ 교과목에서 도출된 ‘fill the red, feel the red’ 프로젝트를 마케팅 및 디자인 프로세스, 결과물을 도출하여 그 과정들을 밝힌다. 아울러 본 연구는 브랜드 스페이스 마케팅에 대한 이론적 고찰을 통해, 브랜드 스페이스(팝업스토어)를 개발 및 스타일링하며 ㈜애경의 화장품 브랜드 ‘LUNA’를 브 랜딩, 스타일, 마케팅적 측면에서 종합적으로 연계하여 도출되는 과정들을 제안함으로서 향후 브랜드 스페이스 디자인과 마케팅 분야에 체계적이고 창의적인 방법들로 결과물을 도출하는데 기초자료로 활용하는데 의의가 있다.

제1유구치의 조기 상실로 인한 공간 변화에 대한 3차원적 분석

김지연,정다운,곽소연,유승은,박기태 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

본 연구는 제1유구치의 조기 상실 시 공간 변화에 대한 분석을 위하여 3-Dimensional Laser Scanner를 이용하여 그 유용성을 평가하고자 하는 예비논문이다. 6세-8세 사이의 제1대구치가 맹출하여 확실한 교합 관계를 이루는 혼합치 열기 어린이 중 편측 제1유구치의 조기 발치가 필요한 6명의 발치 전 후의 모형을 이용하여 다음과 같은 결과를 얻었다. l. 유구치 공간변화는 상악은 실험군과 대조군 모두 현저한 감소를 보이지 않았고, 하악은 실험군에서 3명 중 2명에서 공간감소가 관찰되었다. 2. 치열궁 너비,치열궁 둘레는 초기모형과 최종모형에서 비슷한 값을 보였다. 3. 협설측 경사도 변화는 유견치에서는 상하악 모두 특별한 경향을 보이지 않았으며,제2유구치는 상악은 실험군과 대조군이 유사하였고,하악에서는 3명중 2명에서 실험군이 더 큰 설측경사도 변화를 보였다. 제1대구치는 하악에서 실험군이 더 큰 설측경사도 변화가 관찰되었다. 4. 근원심 경사도 변화 측정에서 유견치는 상하악 모두 특별한 경향이 관찰되지 않았고 제2유구치의 경우 하악에서 실험군이 더 큰 근심경사도 증가가 보였다. 제1대구치는 상악에서 실험군,대조군 모두 원심 경사도가 증가되었다. The purpose of this study was to evaluate a 3-Dimensional laser scanner for the space analysis after loss of a primary first molar. Six children with premature loss of a primary first molar were examined using study models taken before and after the extraction. The results were as follows: 1. There was no change in primary molar space after the extraction of a maxillary primary first molar However, 2 out of 3 children experienced primary molar space loss in extraction side of a mandibular primary first molar. 2. Arch width and arch perimeter showed no difference between initial and final model. 3. All primary canines did not show any changes in inclination. Maxillary primary second molars had similar changes in both extraction and control side. However, 2 out of 3 mandibular primary second molars in extraction side showed more lingual tipping compared to control side. Mandibular permanent first molars tipped more lingually in extraction side. 4. In angulation, primary canines showed nothing of significance. Mandibular primary second molars tipped more mesially in extraction side than in control side. Maxillary permanent first molars have increased distal angulation after extraction of primary first molars in both side.

근피로 후 세 가지 회복 방법

이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.

[PC-0023] Agricultural and fatty acid characteristics in doubled haploid (DH) population derived from microspore of rapeseed (Brassica napus L.) F1 generation

Ji-Eun Lee(Ji-Eun Lee),Da-Hee An(Da-Hee An),Kwang-Soo Kim(Kwang-Soo Kim),Young-Lok Cha(Young-Lok Cha),Dong-Chil Chang(Dong-Chil Chang) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

매체 발달에 따른 음반매장의 입지 현황과 특성 분석

김다은(Da eun Kim),박수지(Su ji park),현시아(Sia Hyun),권지은(Ji Eun Kwon),박지희(Ji hee Park),박채린(Chae lin Park),최다연(Da Yeon Choi) 성신여자대학교 한국지리연구소 2016 應用地理 Vol.- No.33

본 연구는 서울시를 중심으로, 오프라인 음반 매장의 현황과 지리적 분포에 대하여 살펴보았다. 특히 매체의 발달로 음악감상 방식의 변화에 따라 생긴 온라인 음반 매장의 입지에 대해 주목하였다. 직접 현장조사를 통해 서울시 내 음반매장 107곳의 입지와 폐점의 위치를 알아보았다. 또한 실물 매체를 파는 음반 매장을 오프라인 음반 매장과 온라인 음반 매장으로 나누고 매장의 특징에 따라 음반만 판매하는 곳, 대형서점과 연계된 곳, 음반을 제외하고 다른 물품도 같이 취급하여 파는 곳으로 분류하였다. 온라인 음반 매장의 경우 공간적 한계가 없는 온라인 입지의 특성상 매장 수는 오프라인 매장보다 더 많을 것으로 예상된다. 전체 음반매장의 43%가 용산구, 중구, 종로구 3구에 입지하였으며 유동인구가 높은 구에 밀집하여 위치하였다. 음반 매장은 주로 지하철역에서 도보로 10분 이내에 접근이 가능한 역세권과 교통이 편리한 곳에 주로 위치하고 있다. 또한 음반 매장은 용산구의 전자상가나 중구의 회현 지하쇼핑센터 같은 매장, 쇼핑 센터 밀집지역에 높은 분포를 보이는 것을 확인할 수 있다. 종로구, 강남구, 마포구, 노원구 순으로 네 개의 구에 위치한 음반매장이 많이 폐점하였으며 마포구 홍대 부근의 매장도 폐점을 하였다. 그 이유로 MP3같은 새로운 대체재 개발로 인해 오프라인 매장의 매출이 감소하여 온라인 매장으로 입지를 옮긴 것과 젠트리피케이션으로 인한 임대료의 상승을 감당하지 못한 것으로 판단된다. This study analyzes the current status and distributions of the offline record stores and focuses on the position of the online record stores which has changed due to the flow of the media development in Seoul. This study is based on the direct data from field studies, surveys and the existing document data. We had on-site survey 146 places, and checked 107 record stores and 39 going-out-of-business stores in Seoul. The results of this research are summarized as following: 43% of the total record stores are located in the three districts of Yongsan, Junggu, and Jongno, and are concentrated in the high-flow population. The record stores are mainly located in convenient areas with easy access to the station and within a 10-minute walk from the subway stations. Also, record stores are highly distributed in the shopping centers such as Yongsan Electronics Market in Yongsan and Hoehyeon Underground Shopping Center in Jung-gu. Four districts have the largest number of closed stores in the order of Jongno, Gangnam, Mapo, Nowon. Many famous and frequent shoppers in nearby Hongik University in Mapo were not able to avoid closure. A store, which had many famous and frequent customers, could not avoid closing because of the development of new alternatives such as MP3 which had reduced the sales of offline stores and shifted to online stores and the lack of merchants who can"t afford increasing rent due to gentrification. A music store selling real media is divided into an offline music store and an online music store. According to the characteristics of the store, it is classified as places selling only music records, places linked to a large bookstores, and places selling with other items. A total of 162 online music stores could be identified, but the number of online stores is expected to be much larger than that of offline stores due to the nature of online presence without space limitations.

무선센서(Wireless Sensor) 네트워크 환경에서 성능 향상을 위한 Hybrid Mac(Medium Access Channel) 프로토콜(Protocol) 연구 개발

이은지(Lee Eun Ji),최다은(Choi Da Eun),김가영(Kim Ga Young),안종석(Ahn Jong Seok),정은지(Jung Eun Ji),홍예은(Hong Yea Eun) 한국통신학회 2015 한국통신학회 학술대회논문집 Vol.2015 No.11

Predictive capacity of a non-radioisotopic local lymph node assay using flow cytometry, LLNA:BrdU-FCM: Comparison of a cutoff approach and inferential statistics

( Da Eun Kim ),( Hyeri Yang ),( Won Hee Jang ),( Kyoung Mi Jung ),( Miyoung Park ),( Jin Kyu Choi ),( Mi Sook Jung ),( Eun Young Jeon ),( Yong Heo ),( Kyung Wook Yeo ),( Ji Hoon Jo ),( Jung Eun Park ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

In order for a novel test method to be applied for regulatory purposes, its reliability and relevance, I.e., reproducibility and predictive capacity, must be demonstrated, Here, we examine the predictive capacity of a novel non-radioisotopic local lymph node assay, LLNA:BrdU-FCM (5-bromo-2`-deoxyuridine-flow cytome-try), with a cutoff approach and inferential statistics as a prediction model. 22 reference substances in OECDTG429 were tested with a concurrent positive control. Hexylcinnamaldehyde 25%(PC), and the stimulation index(SI) representing the fold increase in lymph node cells over the vehicle control was obtained. The optimal cutoff SI(2.7≤cutoff<3.5), with respect to predictive capacity, was obtained by a receiver operating character-istic curve, which produced 90.9% accuracy for the 22 substances. To address the inter-test variability in respon-siveness, SI values standardized with PC were employed to obtain the optimal percentage cutoff(42.6≤Cutoff<57.3%of PC), which produced 86.4% accuracy. A test subastance may be diagnosed as a sensitizer if a statistically significant increase in Si is elicited. The parametric one-sided t-test and non-parametric Wilcoxon rank-sum test produced 77.3% accuracy. Similarly, a test substance could be defined as a sensitizer if the SI means of the vehicle control, and of the low, middle, and high concentrations were statistically significantly different, which was test-ed using ANOVA or Kruskal-Wallis, with post hoc analysis, Dunnett, or DSCF(Dwass-Steel-Critchlow-Fligner), respectively, depending on the equal variance test, producing 81.8% accuracy. The absolute SI-based cutoff approach produced the best predictive capacity, however the discordant decisions between prediction models need to be examined further.

Variation of Seed Viability among Cone Harvest Times at Two Clonal Seed Orchards of Chamaecyparis obtusa

Da-Eun Gu,Ji-Hee Jeong,Ye-Ji Kim,Kyu-Suk Kang 강원대학교 산림과학연구소 2022 Journal of Forest Science Vol.38 No.4

The timing of seed harvesting is an important decision in the management of seed orchards because it affects seed quality and yield. To investigate the effect of cone harvest time on seed quality and determine the optimal harvesting time, cones were regularly collected in seven times and germination tests were performed at each harvest time in two clonal seed orchards of Chamaecyparis obtusa. As cones developed, the percentage of seed germination increased before cone moisture content began to decrease significantly. The moisture contents of cones were highest at the first collection as 68.3% and 67.3% in Jeju and Gochang seed orchards respectively. At this time, germination speed was slowest, indicating poor seed vigour. The highest germination was found at the second stage in Jeju (36.5%) and at the seventh stage in Gochang (28.6%) seed orchard. The germination speed increased as cone moisture content decreased. Additionally, changes of seed vigour differed among the developmental stages in both seed orchards. Consequently, the optimal cone harvest time of C. obtusa seed orchards in Jeju was early September when high germination percentage was obtained. In Gochang seed orchards, late October was optimal cone harvest time when the germination speed was fast and the cone moisture content decreased.