보론 첨가강에서 연주 냉각속도가 고온연성에 미치는 영향 연구 (주편 코너 크랙 발생 방지 방안 확보 연구)

조경철 ( Kyung Chul Cho1 ),구양모 ( Yang Mo Koo ),박중길 ( Joong Kil Park ) 대한금속재료학회 2008 대한금속·재료학회지 Vol.46 No.6

Pyrantel Pamoate (Combantrin)에 依한 蛔蟲 및 鉤蟲感染의 集團治療

徐丙卨,朴定圭,趙昇烈,姜信榮,魚光本,康世喆,李源宰,李正雨,黃邱一 中央醫學社 1972 中央醫學 Vol.23 No.6

The mass treatment trial of pyrantel pamoate on Ascaris lumbricoides and Ancylostoma duodenale infections was carried out to school-children and inhabitants in rural areas in the central part of Korea. Pyrantel pamoate ("Combantrin") tablet (123 mg base) was administered as a single dose of 10. 0 mg per kg. body weight to a total of 4,198 Ascaris infected cases selected out of 4,436 (41. 6%) positives, who were screened from the total 10,660 collected stool specimens. Of these, only 3,169. cases were followed up after treatment to provide an analysis in this study. Incidentally 161 cases with concomittently infected hookworm were also subjected to assess the efficacy of pyrantel pamoate against A. duodenale. The egg negative conversion rate on A. lumbricoides was 95.3%, and on A. duodenale, 97.5% four weeks following treatment. In failed cases, the egg reduction rates on both of the infections were 97.6% and 97.3% respectively. No side effects and no problem in drug administration were demonstrated. It is concluded that this study confirms the high efficacy of pyrantel pamoate in the treatment of Ascaris lumbricoides and Ancylostoma duodenale infections and also proves the acceptability and safety of this drug in the mass treatment of ascariasis in a population with relatively high worm burden.

KCCH cyclotron neutron 및 60Co r-ray에 의한 인체 말초혈액 임파구의 염색체 이상측정

김성호,김태환,정인용,조철구,고경환,류성렬 대한방사선 방어학회 1992 방사선방어학회지 Vol.17 No.1

KCCH cyclotron neutron(30cCy/min) 및 ??Co γ-ray(210cGy/min)를 시험관내의 정상 인체 말초혈액임파구에 조사하여 염색체이상(dicentric 및 centric ring)을 관찰하고 이의 선량-반응관계식을 linear model(Y=K₁D+a), power-law model(Y=K₂Dⁿ), quadratic model(Y=K₃D²) 및 linear-quadratic model(Y=αD+βD²)을 사용하여 구하고 이들 model중 염색체이상의 측정치와 가장 일치하는 관계식을 근거로 하여 γ -ray에 대한 neutron의 relative biological effectiveness(RBE)를 산출하였다. 염색체 이상(dicentric plus centric ring)의 발생분포는 γ-ray의 경우 linear model(P=0.067)을 제외한 power-law model[Y=(5.81±1.96)×10??, P=0.931], quadratic model [Y=(3.91±0.09)×10?D², P=0.972] 및 linear-quadratic model [Y=(6.55±6.83)×10?D+(3.72±0.22)×10?D², P=0.922]에 적합하였다. Neutron의 경우 linear model [Y=(6.12±0.17)×10?³ D-022, P=0.987]에 가장 일치하였고 quadratic model (P<0.005)을 제외한 power-law model[Y=(2.43±0.70)×10?³D+(1.21±0.39)×10?D², P=0.415]에 비교적 적합하였다. 세포당 0.1-1.5개의 염색체이상을 나타내는 neutron의 γ-ray에 대한 RBE는 2.714±0.408이었다. The frequencies of KCCH cyclotron neutron(30cCy/min) or ??Co γ-ray(210cGy/min)-induced asymmetrical interchanges(dicentrics and centric ring) and acentric fragment(deletion) at several doses were measured in the normal human peripheral blood lymphocytes. Chromosome aberrations were scored at the first nitosis after stimulation with phytohemagglutinin. The neutron and γ-ray data were analysed on liner, power-law, quadratic and linear-quadratic model. When the dicentrics and centric rings of γ-ray datas were pooled and fitted to these model, good fits were obtained to power-law [Y=(5.81±1.96)×10??, P=0.931], quadratic [Y=(3.91±0.09)×10?D², P=0.972] 및 linear-quadratic model [Y=(6.55±6.83)×10?D+(3.72±0.22)×10?D², P=0.922] except for linear model(P=0.067). As in the case of neutron data, the best fits was obtained to the linear model [Y=(6.12±0.17)×10?³ D-022, P=0.987] and good fits were obtained to power-law[Y=(5.36±3.02)×10?⁴D??, P=0.601] and linear-quadratic model [Y=(2.43±0.70)×10?³D+(1.21±0.39)×10?D², P=0.415], except for quadratic model (P<0.005). The relative biological effectiveness(RBE) of neutron compared with γ-ray was estimated by best fitting model. In the asymmetrical interchanges range between 0.1 and 1.5 per cell, the REE was found to be 2.714±0.408.

An Image-guided Radiosurgery for the Treatment of Metastatic Bone Tumors using the CyberKnife Robotic System

Cho, Chul-Koo The Korean Musculoskeletal Tumor Society 2007 대한골관절종양학회지 Vol.13 No.1

Bone is a common site for metastatic spread from many kinds of malignancies. The morbidity associated with this metastatic spread can be significant, including severe pain. When it comes to spinal metastasis, occupying nearly 40% of skeletal metastases, the risks of complications, such as vertebral body collapse, nerve root impingement, or spinal cord compression, are also significant. Because of the necessity of preserving the integrity of the spinal column and the proximity of critical structures, surgical treatment has limitations when durable local control is desired. Radiotherapy, therefore, is often used as an adjunct treatment or as a sole treatment. A considerable limitation of standard radiotherapy is the reported recurrence rate or ineffective palliation of pain, either clinically or symptomatically. This may be due to limited radiation doses to tumor itself because of the proximity of critical structures. CyberKnife is an image-guided robotic radiosurgical system. The image guidance system includes a kilovoltage X-ray imaging source and amorphous silica detectors. The radiation delivery device is a mobile X-band linear accelerator (6 MV) mounted on a robotic arm. Highly conformal fields and hypofractionated radiotherapy schedules are increasingly being used as a means to achieve biologic dose escalation for body tumors. Therefore, we can give much higher doses to the targeted tumor volume with minimizing doses to the surrounding critical structures, resulting in more effective local control and less severe side effects, compared to conventional fractionated radiotherapy. A description of this technology and a review of clinical applications to bone metastases are detailed herein.

Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector

Koo, Bon Chul,Kwon, Mo Sun,Choi, Bok Ryul,Kim, Jin-Hoi,Cho, Seong-Keun,Sohn, Sea Hwan,Cho, Eun Jung,Lee, Hoon Taek,Chang, Wonkyung,Jeon, Iksoo,Park, Jin-Ki,Park, Jae Bok,Kim, Teoan 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The Moloney murine leukemia virus (MoMLV) -based retrovirus vector system has been used most often in gene transfer work, but has been known to cause silencing of the imported gene in transgenic animals. In the present study, using a MoMLV-based retrovirUs vector, we successfully generated a new transgenic chicken line expressing high levels of enhanced green fluorescent protein (eGFP). The level of eGFP expression was conserved after germline transmission and as much as 100μg of eGFP could be detected per 1 mg of tissue protein. DNA sequencing showed that the transgene had been integrated at chromosome 26 of the G₁and G₂generation transgenic chickens. Owing to the stable integration of the transgene, it is. now feasible to produce G₃generation of homozygous eGFP transgenic chickens that will provide 100% transgenic eggs. These results will help establish a useful transgenic chicken model system for studies of embryonic development and for efficient production of transgenic chickens as bioreactors.

Locally Advanced, Unresectable Pancreatic Cancer Treated by Stereotactic Radiation Therapy

Chul-Won Choi(최철원),Mi-Sook Kim(김미숙),Chul-Koo Cho(조철구),Seong-Yul Yoo(류성열),Kwang-Mo Yang(양광모),Hyung-Jun Yoo(유형준),Dong-Han Lee(이동한),Young-Hoon Ji(지영훈),Chul-Ju Han(한철주),Jin Kim(김진),Young-Han Kim(김영한) 대한방사선종양학회 2006 Radiation Oncology Journal Vol.24 No.1

목 적: 국소적으로 진행된, 절제 불가능한 췌장암 치료에 있어 고식적 방사선 치료와 비교하여 CyberKnife (CK)를 이용한 정위 방사선 치료의 생존율 및 급성 독성에 대해 분석하고자 하였다. 대상 및 방법: 2003년 4월부터 2004년 4월까지의, Eastern Cooperative Oncology Group (ECOG) 활동도 3 이하이며 CT 및 PET/CT로 평가하여 원격 전이 없는 국소 진행된 췌장암 환자 19명을 대상으로 하였다. 대상 환자는 점차 방사선량을 증가시키는 방법으로 33 Gy, 36 Gy, 39 Gy를 3분할로 각각 6명, 4명, 9명에서 CK를 이용한 정위 방사선 치료를 시행하였으며, 생존율 및 Radiation Therapeutic Oncology Group (RTOG) acute radiation morbidity criteria에 의한 위장관 독성을 분석하였다. 또한 나이, 성별, ECOG 수행 점수, 항암 치료,우회로 조성술(bypass surgery) 여부, 방사선량, CA19-9, 계획용 표적 체적(planning target volume, PTV), CT상 주위 장기 및 혈관 침범 여부 등을 Log Rank test를 이용하여 예후 인자를 평가하였다.결 과: 본 연구에서 중앙 생존 기간은 11개월, 1년 생존율은 36.8%였다. 추적 조사 기간 중(범위 3∼20개월, 중앙값 10개월) 유의한 위장관 급성 독성은 관찰되지 않았다. 단일 인자 분석에서 계획용 표적 체적만이 유의한 예후 인자로 80 cc 이하인 경우가 80 cc 이상인 경우보다 생존율이 높았으며(p-value<0.05), 나이, 성별, ECOG 수행 점수, 항암 치료, 우회로 조성술, CA19-9 수치, 주위 장기 및 혈관 침범 여부 등에서는 통계적으로 유의한 차이를 보이지 않았다. 다인자 분석에서는 65세 이하인 경우와 PTV 80 cc 이하인 경우에서 생존율이 높았다. 결 론: 고식적 방사선 치료, 고선량 입체조형 방사선 치료(high dose conformal radiation therapy), 수술 중 방사선 치료(intraoperative radiation therapy) 또는 세기 조절 방사선 치료(intensity modulated radiation therapy, IMRT)를 이용한 최근의 결과와 비교하여 CK를 이용한 정위 방사선 치료는 생존율 측면에서 비슷하거나 나은 결과를 보였다. 또한 심각한 부작용은 관찰되지 않았으며 짧은 기간의 치료로 환자에게 편의를 제공할수 있어 결과적으로 삶의 질을 향상시킬 수 있을 것이다. 따라서, 이 새로운 치료 방법은 국소 진행된, 절제불가능한 췌장암 환자에서 심각한 부작용 없는 효과적인 치료가 될 것으로 생각된다. 또한 계획용 표적 체적은 CK 치료의 유용한 예후 인자로 사용될 것이다. Purpose: In order to find out whether stereotactic radiation therapy (RT) using CyberKnife (CK) could improve survival rate and lower acute toxicity compared to conventional RT. Materials and Methods: From April 2003 through April 2004, 19 patients with Eastern Cooperative Oncology Group (ECOG) performance status ≤3 and locally advanced pancreas cancer without distant metastasis, evaluated by CT or PET/CT, were included. We administered stereotactic RT consisting of either 33 Gy, 36 Gy or 39 Gy in 3 fractions to 6, 4 and 9 patients, respectively, in an effort to increase the radiation dose step by step, and analyzed the survival rate and gastrointestinal toxicities by the acute radiation morbidity criteria of Radiation Therapeutic Oncology Group (RTOG). Prognostic factors of age, sex, ECOG performance score, chemotherapy, bypass surgery, radiation dose, CA19-9, planning target volume (PTV), and adjacent organ and vessel invasion on CT scan were evaluated by Log Rank test. Results: The median survival time was 11 months with 1-year survival rate of 36.8%. During follow-up period (range 3∼20 months, median 10 months), no significant gastrointestinal acute toxicity (RTOG grade 3) was observed. In univariate analysis, age, sex, ECOG performance score, chemotherapy, bypass surgery, radiation dose, CA19-9 level, and adjacent organ and vessel invasion did not show any significant changes of survival rate, however, patients with PTV (80 cc showed more favorable survival rate than those with PTV>80 cc (p-value<0.05). In multivariate analysis, age younger than 65 years and PTV>80 cc showed better survival rate. Conclusion: In terms of survival, the efficacy of stereotactic radiation therapy using CK was found to be superior or similar to other recent studies achieved with conventional RT with intensive chemotherapy, high dose conformal RT, intraoperative RT (IORT), or intensity modulated RT (IMRT). Furthermore, severe toxicity was not observed. Short treatment time in relation to the short life expectancy gave patients more convenience and, finally, quality of life would be increased. Consequently, this could be regarded as an effective novel treatment modality for locally advanced, unresectable pancreas cancer. PTV would be a helpful prognostic factor for CK.

Role of preoperative RT in external genitalia malignancy

Cho, Chul Koo 대한부인종양 콜포스코피학회 1995 Journal of Gynecologic Oncology Vol.6 No.4

Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV‐ based retrovirus vector

Chul Koo, Bon,Sun Kwon, Mo,Ryul Choi, Bok,Kim, Jin‐,Hoi,Cho, Seong‐,Keun,Hwan Sohn, Sea,Jung Cho, Eun,Taek Lee, Hoon,Chang, Wonkyung,Jeon, Iksoo,Park, Jin‐,Ki,Bok Park, Jae,Kim, Teoa Federation of American Society for Experimental Bi 2006 The FASEB Journal Vol.20 No.13

<P>The Moloney murine leukemia virus (MoMLV) -based retrovirus vector system has been used most often in gene transfer work, but has been known to cause silencing of the imported gene in transgenic animals. In the present study, using a MoMLV-based retrovirus vector, we successfully generated a new transgenic chicken line expressing high levels of enhanced green fluorescent protein (eGFP). The level of eGFP expression was conserved after germline transmission and as much as 100 microg of eGFP could be detected per 1 mg of tissue protein. DNA sequencing showed that the transgene had been integrated at chromosome 26 of the G1 and G2 generation transgenic chickens. Owing to the stable integration of the transgene, it is now feasible to produce G3 generation of homozygous eGFP transgenic chickens that will provide 100% transgenic eggs. These results will help establish a useful transgenic chicken model system for studies of embryonic development and for efficient production of transgenic chickens as bioreactors.</P>

Demographic and Clinical Characteristics of Primary Frozen Shoulder in a Korean Population

Chul-Hyun Cho,Tae Won Koo,Nam-Su Cho,Kyoung-Jin Park,Bong Gun Lee,Dongju Shin,Sungwook Choi,Seung-Hyun Cho,Myung-Sun Kim,Sang-Hun Ko,Chul-Hong Kim,Jin-Young Park,Yon-Sik Yoo 대한견주관절의학회 2015 대한견주관절의학회지 Vol.18 No.3

Background: The aim of this study was to investigate the demographic and clinical characteristics of patients with primary frozen shoulder in a Korean population. Methods: A total of 1,373 patients whose shoulders were diagnosed with primary frozen shoulder across 11 resident-training hospitals were reviewed retrospectively. Various demographic characteristics and clinical characteristics according to gender and presence of diabetes were evaluated. Results: The average age of patients was 55.4 years. Gender proportion was 58.3% females and 41.7% males. The frozen shoulder involved the non-dominant arm in 60.7% of patients and the bilateral arms in 17.6% of patients. The average duration of symptoms was 8.9 months, and 51.3% of patients had experienced nocturnal pain. Comorbidities associated with frozen shoulder in our sample of patients included diabetes (18.7%), cardiovascular diseases (17.7%), thyroid diseases (5.4%), and cerebrovascular diseases (3.6%). The diabetic group was correlated with the following demographic and clinical characteristics: old age, involvement of the dominant arm, nocturnal pain, long duration of symptoms, and no history of trauma. Further, we found that, in males, having a frozen shoulder was significantly correlated with a history of trauma; in females, having a frozen shoulder was significantly correlated with having thyroid diseases. Conclusions: These demographic data of primary frozen shoulder in the Korean population were consistent with those of previously reported epidemiologic studies. Primary frozen shoulder with diabetes was correlated with old age, bilateral involvement, long duration of symptoms, and nocturnal pain.

Role of CyberKnife in the Field of Surgical Oncology : Focusing on Colorectal Cancers

Chul-Koo Cho 대한외과학회 2004 대한외과학회 학술대회 초록집 Vol.2004 No.5