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미세절제술과 비교 유전자 보합법에 의한 각종 종양에서의 유전자 변화에 관한 연구
구선회,신소영,임춘화,전영미,이윤이,김진만 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2
For the evaluation of oncogenesis, progression and prognosis of cancer, CGH is an important technique, because this technique is economic due to utilization of only one probe and lack of culture, screening mathod of whole genome and possibility of retrospective and prospective study. By the CGH, genornic variation of 20 breast cancer tissues, 23 stomach cancer tissues and 16 bladder cancer tissues were analyzed. The results were as followes ; 1. breast cancers The CGH results showed gains on chromosomes 8q(40%), lq(30%), 17q(15%), 20q(15%), 18q (15%), 5p(15%), and 13q(15%). The Deletions were on chromosomes 17p(45%) and 22q(20%). High-level amplifications(green/red ratio >1.5) were noted on chromosomes 1p31, iq, 3q25-qter, 5p, 7q31-qter, 8q, 9q22-qter, 10p, l1p, 11q22-qter, 12p, 12q24, 14g21-qter, 15q23-qter, 17q, 18p, 18q12-qter, 20p, and 20q. By comparison with infiltrating ductal carcinoma, the two medullary carcinomas showed high-level amplification on chromosomes iq3l, lq, 8q, 10p, 11p and 12p. 2. stomach cancers 1) Usual amplification sites of genome were lq, 13q, 17q, 20p,q. 2) 17p was the most common deletion site. The other sites of the deletion were lq, 4q. 3) In intestinal type of stomach cancer, genomic variation is more common than diffuse type. 4) In the cases of no evidence of lymph node metastasis, deletion of 17p is absent but amplification of 8q is obvious in the case of lymph node metastasis. 3. bladder cancers Common amplification of copy numbers of DNA sequences by CGH were seen at 1q, 3q, 4q, 5p, 6pq, 7p, 8q, 11q, 12q, 13q, 17q, 18q and 20pq(more than 20% of cases). High level amplification was noted at 1p32, 3p2l, 3q24, 4q26, 8q21-ter, 11q14-22, 12q15-21, 12q21-24, 13q 21-31, 17q22, and 18q22. Deletions were noted at 2q21-qter, 4q13-23, 5q, 8p12-22, 9pq, 11p13-15 (more than 20% of cases).
Exercise-Enhancing Effect of Curcuma Longa L. Extracts in Relation toAntioxidant Capacity
( Mi So Lee ),( Jeong Jin Park ),( Yang Hee You ),( Kwon Tack Hwang ),( Jeong Min Lee ),( Kyung Mi Kim ),( Seong Koo Kang ),( Hee Jong Chung ),( Woo Jin Jun ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2008 한국식품저장유통학회 학술대회 Vol.2008 No.-
Determine the impact of different types of SARS-Cov-2 vaccines on IVF outcome
( So Yeon Shin ),( Hwa Seon Koo ),( Jee Hyun Kim ),( Ji Hyang Kim ),( Chan Park ),( Hwang Kwon ),( Dong Hee Choi ),( Seon Mi Cho ),( Ji Eun Shin ) 대한산부인과학회 2022 대한산부인과학회 학술대회 Vol.108 No.-
Objective: To investigate whether the immune response to the different types of vaccines and infection against SARS-Cov-2 virus affects ovarian follicle and its function and to evaluate whether the ART process affects the immunity obtained from either SARS-Cov-2 vaccines or infection. Methods: This is a prospective cohort study, included 45 consecutive IVF patients visiting private university fertility center, either immune with vaccination, previous infection or control. Serum and follicular fluid were collected to analyze the titer of anti-SARS-Cov-2 immunoglobulin G(IgG) as well as concentration of estrogen and progesterone both on cycle day 2-3 and the day of trigger. Main outcomes were serum immunoglobulin change during IVF procedure, and follicular function of steroidogenesis in serum and follicular fluid. Results: Of 45 women included in this study, 23 were vaccinated with Pfizer-BioNTech mRNA SARS-Cov-2 vaccine, 7 were vaccinated with other types of vaccines including Moderna and cross-inoculated with AstraZeneca and Janssen/J&J vaccines, 10 patients were known PCR test-positive to COVID-19 and recovered from the infection more than two weeks without any upper respiratory symptoms. There was no difference between serum and follicular fluid titer of spike protein IgG for SARS-Cov-2. However, relatively lower titer of serum and follicular IgG was more frequently observed in Pfizer-BioNTech vaccine group(45%, 14.3%, 20%, 20%; Pfizer, other types of vaccine, COVID-19 positive, control) with no statistical significance(p=0.351). There was no difference between serum estrogen and progesterone on Cycle day 2-3 or the day of trigger as the hormones in follicular fluid. Conclusion: The study findings suggest that procedure of controlled ovarian hyperstimulation does not affect the titer of spike protein IgG for COVID 19. In addition, there was no significant difference in steroidogenesis between groups depending on the presence or absence of immunity and how the immunity was acquired.
( So Yeon Park ),( Moon Young Kim ),( Soon Koo Baik ),( Sang Ok Kwon ),( Hyoun A Kim ),( Hye Won Kang ),( Hye Kyung Hwang ),( Jung Mi Jo ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: Measurement of hepatic venous pressure gradient (HVPG) is a gold standard for the assessment of the presence of portal hypertension and correlates with the occurrence of its complications. Especially, HVPG is most reliable indicator in estimation of effectiveness of nonselective beta-blocker treatment. However, HVPG is limited in its clinical application in estimation of therapeutic response because of its invasiveness and difficulty in bed-side repeated measurement. Liver stiffness measurement (LSM) has been proposed as a noninvasive method for the prediction of the severity of hepatic fibrosis. In this study, we evaluated the relationship between the interval change of LSM and HVPG (ΔLSM and ΔHVPG) after 3months propranolol treatment and predictive value of LSM for estimation of the response of HVPG to propranolol. Methods: LSM with transient elastography and HVPG were performed at baseline and after 3month propranolol treatment in 43 consecutive cirrhotic patients (M: F = 38: 5). Therapeutic response defined as HVPG reduction more than 20% compared to baseline or below 12mmHg. Linear regression analysis was performed for evaluation of relationship between ΔLSM [%, (baseline LSM - follow-up LSM) / baseline LSM x 100] and Δ HVPG [%, (baseline HVPG - follow-up HVPG) / baseline HVPG x 100]. Diagnostic values were calculated based on ROC curves. Results: The etiologies of cirrhosis were composed of alcohol and HBV (29 and 14 patients, respectively). The baseline mean HVPG and LSM were 17.0 ± 4.5mmHg and 40.1 ± 18.1kPa, respectively and these showed significant correlation (r2 = 0.333, p < 0.0001). Follow up measurement of HVPG and LSM (mean value is 12.3 ± 5.2mmHg and 30.3 ± 17.4kPa, respectively) also showed significant correlation (r2 = 0.615, p < 0.0001). A strong positive relationship between ΔLSM (%) and ΔHVPG (%) was also observed in the overall population (r2 = 0.420, p < 0.0001). Therapeutic response was achieved in 30 patients (69.8%). The area under ROC curve (AUROC) for the prediction of therapeutic response was 0.826 and sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) according to ΔLSM (%) cutoff values of 10% were 83.3, 61.5, 83.3 and 61.5% respectively. Conclusions: The interval change of LSM showed significant correlation with the change of HVPG after propranolol treatment and LSM could be useful non-invasive method for estimation of the anti-portal hypertensive therapeutic response in patients with liver cirrhosis.
Koo, So-My,Kim, Song Yee,Choi, Sun Mi,Lee, Hyun-Kyung,Korean Interstitial Lung Diseases Study Group The Korean Academy of Tuberculosis and Respiratory 2019 Tuberculosis and Respiratory Diseases Vol.82 No.4
Connective tissue disease (CTD) is a collection of disorders characterized by various signs and symptoms such as circulation of autoantibodies in the entire system causing damage to internal organs. Interstitial lung disease (ILD) which is associated with CTD is referred to as CTD-ILD. Patients diagnosed with ILD should be thoroughly examined for the cooccurrence of CTD, since the treatment procedures and prognosis of CTD-ILD are vary from those of idiopathic interstitial pneumonia. The representative types of CTD which may accompany ILD include rheumatoid arthritis, systemic sclerosis (SSc), Sjogren's syndrome, mixed CTD, idiopathic inflammatory myopathies, and systemic lupus erythematous. Of these, ILD most frequently co-exists with SSc. If an ILD is observed in the chest, high resolution computed tomography and specific diagnostic criteria for any type of CTD are met, then a diagnosis of CTD-ILD is made. It is challenging to conduct a properly designed randomized study on CTD-ILD, due to low incidence. Therefore, CTD-ILD treatment approach is yet to been established in absence of randomized controlled clinical trials, with the exception of SSc-ILD. When a patient is presented with acute CTD-ILD or if symptoms occur due to progression of the disease, steroid and immunosuppressive therapy are generally considered.
Techzyme® HBsAg, Anti-HBs 시약의 유용성 평가
신소영,김문희,박윤미,권계철,구선희,박종우 대한임상검사정도관리학회 2001 臨床檢査와 精度管理 Vol.23 No.2
배경:B형 간염은 우리나라에서 만연하고 있는 질환중의 하나이며, 이에 대한 혈청학회 검사는 B형 간염 진단에 중요하다. 최근 개발된 국산시약인 Techzyme®(Asan, Korea)을 이용하여 B형 간염의 표면 항원(HBsAg ), 항체 (Anti-HBs)의 임상적 유용성을 검토하고자 하였다.<br> 방법: 2001년 1월부터 3월 사이에 충남대학교 병원 임상병리과에 의뢰된 150검체를 대상으로 민감도와 특이도를 검사하였으며 Techzyme®음성대조 물질과 양성 대조물질을 이용하여 검사 중 정밀도와 검사간 정밀도를 구하였다. 고농도 환자 혈청을 단계 희석한 후 직선성을 구하였다.<br> 결과:정밀도에서 음성 표준물질인 경우 일간 재현성 검사와 일일 재현성 검사에서 HBs Ag은 변이계수가 7.48%와 11.2%이고 Anti-HBs는 7.02%와 14.6%이었다. 양성표준물질인 경우 일간 재현성 검사와 일일 재현성 검사에서 HBsAg은 변이계수가 2.34%와 4.14%이고 Anti-HBs는 2.43%와 4.97%이었다. 직선성의 평가에서는 HBsAg의 경우 R<sup>2</sup>=0.9903이고 Anti-HBs는 R<sup>2</sup>=0.9977이었다. 항원, 항체 검사에서 94%, 92%의 일치율을 보였고 민감도와 특이도는 항원 검사에서는 100%, 100%이고 항체 검사는 각각 95.6%,96.3%이었다.<br>결론: 새로 개발된 국산시약 Techzyme®은 우수한 정밀도와 직선성을 보였고 민감도와 특이도가 높아 임상적으로 유용하게 사용될 수 있으리라 생각된다. Background: The hepatitis B virus was one of the most prevalent infectious agent in Korea. Serologic assays of the detection for hepatitis B surface antigen (HBsAg) and hepatitis B antibody(Anti-HBs) were useful markers for the diagnosis of viral hepatitis. Some of domestic hepatitis enzyme immunoassay (EIA) reagents have been developed. We analyzed one of them, Techzyme® ELISA 3.0 HBsAg and Anti-HBs (Asan, Korea), to evaluate the clinical usefulness.<br> Methods: The precision, linearity, sensitivity and specificity of Techzyme® HBsAg and Anti-HBs were evaluated with 150 clinical specimens from January to March, 2001.<br> Results: The within-run and between-run coefficient of variance (C.V.) HBsAg were 7.48% and 11.12% in negative control and were 2.34% and 4.14% in positive control, respectively. The within-run and between-run CV for Anti-HBs were 7.02% and 14.6 in negative control and 2.43% and 4.97% in positive control. On linearity test, the R<sup>2</sup> for HBsAg was 0.9903 and the R<sup>2</sup> for Anti-HBs was 0.9977. The concordant results were 94% and 92% for HBsAg and Anti-HBs with conventional reagents, respectively. The sensitivity and specificity for HBsAg were 100%, both. And the sensitivity and specificity for Anti-HBs were 95.6% and 96.3%, respectively.<br> Conclusions:In conclusion, because of high sensitivity, specificity and high linearity, Techzyme® ELISA 3.0 HBsAg and Anti-HBs appeared to be suitable for detecting HBs Ag and Anti-HBs in clinical laboratories.