Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

가스상 대기오염물질에 의한 종이 기록물의 가속열화 특성 연구

정소윤(So-Yoon Jeong),전수연(Soo-Yeon Jeon),백소라(So-Ra Baek),정현석(Hyun-Seok Jeong),이진희(Jin-Hee Lee),김형진(Hyoung-Jin Kim) 한국펄프·종이공학회 2015 펄프.종이기술 Vol.47 No.4

Paper records were generally degraded by some factors from atmospheric environments, like temperature, relative humidity or air pollutants. In this study, the degradation behavior of paper records by single or mixed gases of NO₂, SO₂, HCHO and TVOC was evaluated. The mechanical, optical and chemical properties of 4 kinds of paper (acid and neutral-based printing paper, traditional Hanji, and filter paper) were directly and indirectly affected by gaseous harmful materials. The brightness and L<SUP>*</SUP> value in all papers were slightly increased by accelerated aging under gaseous HCHO and TVOCs, but highly decreased by conditions under gases NO₂ and SO₂. The optical properties of paper records were most vulnerable in acid-based paper and high stable in filter paper and traditional Hanji by air pollutant degradation. The aging treatments under mixed gas pollutants including NO₂ resulted in decrease of physical, mechanical and optical properties of paper, so it was supposed that the concentration of NO₂ gas would be strictly controlled for optimum indoor air quality management in domestic storage centers for paper records.

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach

So‑Ra Han,Sung‑Min Jang,Young Min Chi,Byeollee Kim,정상희,Yung Mi Lee,Jun Uetake,Jun Hyuck Lee,Hyun Park,오태진 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confrmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by fnding new genes that degradation the pectin

The Effects of Participation in Physical Activity Programs Based on Self-Determination on Developmentally Disabled Children`s Gross Motor Skills, Concentration, and Communicative Competence

( So Hyung Kim ),( Hye Min Kim ),( Hyun Su Lee ) 한국체육학회 2015 국제스포츠과학 학술대회 Vol.2015 No.1

Purpose: The purpose of this study is to provide children with developmental disorders with physical activities that are preferred based on self-determination theory and verifying the effects. This study is a follow-up study of one conducted by So Hyung Kim, Hye-Min Kim, and Hyun-Su Lee(2015) and it chose a type among preference types classified through Q methodologies. Afterwards, programs were developed based on physical activities preferred by children who fit that type, and then applied. Method: The study selected 28 children meeting the criteria through purposive sampling from six- to twelve-year-old disabled children living in C City and attending a kindergarten, special- education school, or general school`s special class. The subjects were made to classify 24 adapted physical activity cards developed for this research, and their preferred types were analyzed with QUANL PC Program. The type (Type 1) that had the most subjects in Type Q conducted the adapted physical activity they preferred twice a week, for 50 minutes in each session, for total 15 weeks. To examine the effectiveness of the program, the ATA concentration test, frostig-MSTB, percentage of correct consonants test, and maximal phonation time test were performed. Lastly, to analyze data for verifying hypotheses, SPSS for Window 10.0 was employed for repeated measured two-way ANOVA. Result: First, developmentally disabled children participating in the adapted physical activity program based on their self-determination showed more change in bending forward, standing long jump, shuttle run walking on a balance beam, balancing on one leg than does not in gross motor skills. Second, developmentally disabled children participating in the adapted physical activity program based on their self-determination showed more change in omission error than those not in concentration skills. Third, developmentally disabled children participating in the adapted physical activity program based on their self-determination did not show more change in the percentage of correct consonants (%) and in maximal phonation time (MPT) which is one of the aerodynamic outcomes than those not in communicative competence. Conclusion: Research results allowed recognition of the academic importance of the self-determination of disabled children through program applications made by the decisions of the disabled children themselves. Efforts to develop self-determination enhancement programs for disabled children in physical training fields will also be needed.

대기 중 유해성분(NO₂, SO₂, HCHO)에 의해 열화된 종이의 물리적 특성

백소라(So Ra Beck),정소윤(So Yoon Jeong),정현석(Hyun Suk Jung),이진희(Jin Hee Lee),김형진(Hyoung Jin Kim),김신도(Shin Do Kim) 한국펄프·종이공학회 2014 한국펄프·종이공학회 학술발표논문집 Vol.2014 No.10

간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.

A Review on the Application of Nanotechnology in Food Processing and Packaging

Seong-In Cho,Yong-Rok Kim,Joon Woo Lee,Dae-Sup So,Yong-Jin Cho,Hyun Kwon Suh,Tu San Park,Seoung-Im Oh,Ji-Eun Im 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.4

Currently, nanotechnology is widely applied in various industrial fields and is rapidly emerging as a promising future technology. In food industries, nanotechnology is used to enhance food quality and safety. Numerous cutting-edge studies on the advantages of nanotechnology have been conducted in the fields of food processing, food ingredients and additives, food packaging, and food engineering for optimal health. The market for these areas of research has grown steadily, and is expected to continue to do so. Because of this, R&D for nanotechnology that can be used effectively in food industries is being performed by several companies, as well as in academic research institutions around the world. This review describes the recent global R&D trends that have been in progress for two key areas: food processing and food packaging.

사업장(事業場) 근로자(勤勞者)의 사상체질(四象體質)에 따른 생활습관(生活習慣), 자각증상(自覺症狀), 건강인식(健康認識)에 관(關)한 연구(硏究)

서명효,강홍구,장두섭,왕명자,차남현,김삼태,권소희,정해경,송용선,이기남,Seo Muoung-Hoy,Kang Hong-Goo,Jahng Doo-Sub,Wang Myoung-Ja,Cha Nam-Hyun,Kim Sam-Tae,Kwon So-Hee,Jung Hae-Kyoung,Song Yung-Sun,Lee Ki-Nam 대한예방한의학회 2003 대한예방한의학회지 Vol.7 No.2

The study was conducted to evaluate the difference of life style, subjective symptoms and health cognition depending on the Sasang Constitution in the industrial workers. The results were as follows ; Constitutional distribution among exposed group were Tae-eum In(38.4%), So-eum In(35.2%), and So-yang In(26.4%) and it was turned out that Tae-eum In took a higher portion of Sasang constitution than other constitutions in technician workers. 'Exercise' and 'Obesity' were found most in Tae-eum In, In So-eum In there were many 'Normal' weight and 'Having digestive disorders' were found most in So-eum In. There were no significant difference in the health cognition among the constitutions. The closer to 'normal' weight, the higher prevalence in 'having' concern about health condition and the more 'normal' condition in sleeping, the higher prevalence of 'having' self-confident in present health.