HPLC 방법에 의한 신생아 요중 아미노산 정량 분석

권계철 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Recently neonatal screening tests including the Guthrie test for evaluation of metabolic disorders have become important. In the case of abnormal results in screening tests, accurate concentrations of amino acids in plasma or urine are needed to diagnose specific metabolic disorders. However reference ranges for urine amino acid concentrations in Korean neonates have not been determined. This study was performed to determine urinary amino acid concentrations in 60 Korean neonates using a Waters^TM HPLC amino acid system. The results obtained were summarized as follows : 1. Standard solutions showed clear cut peaks of 31 kinds of amino acids. 2. Analyses of eight clinically important amino acids showed that valine was detected most frequently and phenylalanine was the least detected. 3. Quantitations of eight clinically important amino acids showed that the mean concentration of leucine was 554.9 μmole/ml which was the highest, and tyrosine was 108.25 μmole/ml. Concentrations of the remaining six amino acids were low. In conclusion, concentrations of eight amino acids. which arc important for screening of neonatal metabolic disoders were analyzed. These results provide the basis for establishment of a reliable urinary amino acid reference data.

자동면역분석기 Architect의 유용성 평가

권계철 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

The newly developed immunoassay analyzer, Architect(Abbott, USA) has been chosen as a method of tumor and viral markers because it is easily applicable to TLA(total laboratory automation)system. Laboratory tests can be performed at TLA system in some large hospitals for rapid, accurate results and decreased labor. The linearity, accuracy, precision and correlation study with conventional EIA method was performed to evaluate the usefulness of Architect immunoanalyzer concerning HCV test. The results were as follows : 1. Within run coefficient of variation(CV) at 20 runs with control serum of HCV was 2.07%, 2. Between run CV at 20 days with control serum of HCV was 3.73%. 3. The percentage of variation in HCV was 2.86% to evaluate accuracy with the control serum measured 20 times. 4. The linearity of HCV was evaluated with the control serum diluted 100:0, 80:20, 60:40, 40:60, 0:100. The regression equation of those were Y=0.361X-0.190(R=0.995). 5. The correlation of the HCV with conventional EIA was Y=0.867X-0.102(R=0.948). The architect immunoanalyzer was adequate to perform viral marker tests in TLA system because of its high precision, good accuracy, linearity and high correlation with conventional EIA.

FPIA 법을 이용한 Cyclosporine A 혈중 농도 측정에 관한 연구

권계철 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

A consistent improvement in the outcome of organ transplantation has been achieved since cyclosporine was first used in clinical practice. Rapid and precise cyclosporine measurements are necessory to maximize immunosuppression and minimize toxicity. The author had analyzed and compared the cyclosporine concentration in serum and whole blood specimens from renal transplant patients using fluorescence polarization immunoassay(FPIA) with Abbott TDx System. The results obtained were summarized as follows : 1. The mean CsA concentration in whole blood was 180.434± 122.16 ㎍/L. 2. The mean CsA concentration in serum was 93.298± 60.988 ㎍/L. 3. The CsA concentration of whole blood was 1.9 times higher than that of serum 4. The regression equation of CsA concentration between whole blood and serum was y= 1.90x+3. 0408 with a correlation coefficient(r) of 0.94583, based on 41 determination and results had a high correlation. In conclusion, monoclonal FPIA method with whole blood was recommended as therapeutic drug monitoring of CsA because temperature and hematocrit variables are absent in whole blood matrix. With monoclonal FPIA method using whole blood specimen, concentration of cyclosporin could be determined accurately.

인증 심사 분야별 사례 보고

권계철 대한임상병리사협회 2011 임상검사정보학회 발표자료집 Vol.2011 No.-

급성 임파구성 백혈병의 면역학적 분류 및 예후에 관한 연구

권계철,구선회 대한임상병리학회 1990 대한임상병리학회지 Vol.10 No.1

Coulter(r) Model Stacker와 수동방법에 의한 감별혈구의 비교 연구

권계철,구선회,박종우 대한임상검사정도관리학회 1989 臨床檢査와 精度管理 Vol.11 No.1

임상화학 검사의 Delta Check System 개발 및 정도관리 효율성 검토

권계철,임춘화,김문희,박연보 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : The application of the delta check in tests in general chemistry detects both random and clerical errors, thus enhancing the reliability of tests. However, since this approach adds the burden of confirming the results to the laboratory, it has the disadvantage of lengthening the turn around time of tests. We speculated that an online delta check system needed to reduce the turn around time. We developed delta quality control system and evaluated its effectiveness of quality control in clinical chemistry. Methods : We developed an online delta check system based on the client-server paradigm. We used IBM PentiumⅢ PC as server and as clients. The database system used was powerbuilder 6.0. Results : In the system we developed, delta screening was performed when test results were input to the computer. The decision over the delta screening was made by comparing it against patient's clinical information and cumulative results within the same screen. Conclusions : The developed delta check method made it available to reduce the turn around time previously spent on delta screening by eliminating the batch processing of tests which was needed in previous approaches separate query cumulative results and patient's clinical informations for screening purposes.

건식 화학 분석기 Ektachem 750의 평가

권계철,송인숙 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

We evaluated a multichannel dry chemical analyzer "Ektachem 750 XRC" for emergency tests according to NCCLS guidelines. Results were fo lowed as below. 1. The evaluation of between-run precision and within-run precision about Na^+, K^+, C1^-, glucose, Cr, ALT, ammonia and amylase revealed high coefficients of variation. 2. In the evaluation of linearity, K^+, glucose, ALT and Cr showed high linearity as r=0.99, r=0.99, r=0.99 and r=0.94 respectively. 3. The correlation studies about Na^+, K^+, Cl^- were performer with NOVA 13. Correlation coefficients of Na^+, K^+ and Cl^- were r=0.93, r=0.99 and r=0.96 respectively. The correlation coefficients of ALT, glucose and Cr with HITACHI 736-20 were r= 0.99, r=0.99 and r=0.94 respectively. We concluded that Kodak Ektachem 750 XRC was suitable as emergency chemistry autoanalyzer because of high precision, linearity and relatively high correlation coefficients.

응급 자동 화학 분석기 Synchron CX3의 평가

권계철,신연식 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

We evaluated a multichannel chemistry autoanalyzer "CX3" for emergency tests according to NCCLS guidelines. Results were as followed 1. The evaluation of day to day precision, between run precision and within run precision about Na, K, Cl, glucose, Cr, BUN and Ca revealed high coefficents of variation. 2. In the evaluation of linearity, Na and Cl showed high linearity as r=0.994 and r=0.998. 3. The correlation studies were performed with Nova 13. Correlation coefficients of Na K and Cl were 0.68, 0.98 and 0.84 respectively. We concluded that "CX3" was suitable as emergency chemistry autoanalyzer because of high precision, linearity and relatively high correlation coefficients.

두단계 중합효소연쇄반응을 이용한 C형 간염 진단

권계철,구선회,박종우 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Hepatitis C infection is known to be a very serious disease and high risk of transmission by nonparenteral route. But immunoassay method was less sensitive and specific than that of PCR method. The extraction of RNA is largely important to amplification of hepatitis C virus. This study was performed to evaluate the new reagent, Tri-reagent, which was used to extract RNA compared to the conventional method : heat and phenol-chloroform extraction method. The results were as follows 1. Mean concentration of extracted RNA by Tri-reagent method was 6135.3 mg/ml. 2. Mean concentration of extracted RNA by phenol chloroform method was 419.0 mg/ml. 3. Mean concentration of extracted RNA by heat method was 691.7 mg/ml. 4. PCR products by Tri-reagent were the most distinctive than those by other two methods The mean concentration of extracted RNA by Tri-reagent method was much higher than that of RNA by phenol - chloroform and heat method. In conclusion, the usage of Tri-reagent was rapid and succesful to extract of RNA compared to the conventional extraction methods.