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구선회,조혜현,성지연,권계철,임진숙 대한임상미생물학회 2011 Annals of clinical microbiology Vol.14 No.2
Background: Enterococcus faecium has emerged as an important nosocomial pathogen worldwide, and this trend has been associated with the dissemination of a genetic lineage designated clonal complex 17 (CC17). In the present study, characterization of the glycopeptide resistance mechanism, genetic relatedness, and pathogenicity in isolates of vancomycin-resistant E. faecium in the Chungcheong area were investigated. Methods: A total of 37 consecutive, non-duplicate, vancomycin-resistant E. faecium were isolated at three university hospitals in the Chungcheong area. The mechanism of glycopeptide resistance and pathogenicity factors were studied using PCR, and the genetic relatedness was determined via multilocus sequence type and esp repeat profile analysis. Additionally, the quinolone resistance-determining regions of parC and gyrA were sequenced to identify mutations involved in ciprofloxacin resistance. Results: Two genotypes of VRE were confirmed: VanAphenotype vanA genotype VRE (25 isolates) and VanB-phenotype vanA genotype VRE (12 isolates). MLST analysis revealed five sequence types. A significant result was that ST414 and CNS4 (4-1-1-1-1-1-1) were considered as belonging to CC17. The esp and hyl genes were found in 100% and 86.4% of the isolates, respectively. A total of 37 isolates showed genetic mutations in parC and gyrA. Conclusion: All isolated strains in the present study belonged to one of the CC17 genotypes including ST414 and CNS4 (4-1-1-1-1-1-1), which were not previously detected in Korea. The combination of MLST and the esp gene repeat profiles can be useful for genetic characterization of VREF isolates with regard to the evolutionary process and epidemiology of the clones.
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구선회,임춘화,전영미,김성호 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2
Cytogenetic results and genetic alterations are closely related with tumorigenesis, progression and promotion of cancers. With the deepening of basic biologic understanding of neoplastic mechanisms, the clinical usefulness of various cytogenetic abnormalities as diagnostic and prognostic aids has been appreciated. Ten cases of brain tumors(2 Glioblastoma multiforme, 3 meningioma, 1 ependymoma, 1 gliosarcoma, 1 astrocytoma, and 1 metastatic tumor) were studied and 8 cases were possible for karyotyping . Four patients showed abnormal karyotypes. Structural and numerical abnormalities were detected. Karyotyping of 2 Glioblastoma multiforme showed trisomy 7, loss of chromosome 10 and loss of chromosome 8. Structural anomalies were detected on chromosome 1q, and 7. Many many marker chromosomes were also detected. One gliosarcoma showed many numerical anomalies, such as +7, +8, +17, and +mar. Meningioma cells studied were normal daryotyping. Although the cases were too small to correlate with the tumorigenesis, but histologically poor grade type of tumors showed very complex karyotypes.
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미세절제술과 비교 유전자 보합법에 의한 각종 종양에서의 유전자 변화에 관한 연구
구선회,신소영,임춘화,전영미,이윤이,김진만 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2
For the evaluation of oncogenesis, progression and prognosis of cancer, CGH is an important technique, because this technique is economic due to utilization of only one probe and lack of culture, screening mathod of whole genome and possibility of retrospective and prospective study. By the CGH, genornic variation of 20 breast cancer tissues, 23 stomach cancer tissues and 16 bladder cancer tissues were analyzed. The results were as followes ; 1. breast cancers The CGH results showed gains on chromosomes 8q(40%), lq(30%), 17q(15%), 20q(15%), 18q (15%), 5p(15%), and 13q(15%). The Deletions were on chromosomes 17p(45%) and 22q(20%). High-level amplifications(green/red ratio >1.5) were noted on chromosomes 1p31, iq, 3q25-qter, 5p, 7q31-qter, 8q, 9q22-qter, 10p, l1p, 11q22-qter, 12p, 12q24, 14g21-qter, 15q23-qter, 17q, 18p, 18q12-qter, 20p, and 20q. By comparison with infiltrating ductal carcinoma, the two medullary carcinomas showed high-level amplification on chromosomes iq3l, lq, 8q, 10p, 11p and 12p. 2. stomach cancers 1) Usual amplification sites of genome were lq, 13q, 17q, 20p,q. 2) 17p was the most common deletion site. The other sites of the deletion were lq, 4q. 3) In intestinal type of stomach cancer, genomic variation is more common than diffuse type. 4) In the cases of no evidence of lymph node metastasis, deletion of 17p is absent but amplification of 8q is obvious in the case of lymph node metastasis. 3. bladder cancers Common amplification of copy numbers of DNA sequences by CGH were seen at 1q, 3q, 4q, 5p, 6pq, 7p, 8q, 11q, 12q, 13q, 17q, 18q and 20pq(more than 20% of cases). High level amplification was noted at 1p32, 3p2l, 3q24, 4q26, 8q21-ter, 11q14-22, 12q15-21, 12q21-24, 13q 21-31, 17q22, and 18q22. Deletions were noted at 2q21-qter, 4q13-23, 5q, 8p12-22, 9pq, 11p13-15 (more than 20% of cases).
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Fluorescence in situ Hybridizaton에 의한 염색체 이상의 절단점의 규명
구선회,송인숙 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2
Fluorescence in situ hybrididzation(FISH) is a novel molecular genetic technique. It used for gene mapping in researches and in clinical cytogenetic fields. We perfomed FISH using various kinds of probes for the determination of breakpoints in chromosomal abnormalities. Following results were obtained: 1. Determination of breakpoint in complex translocations. FISH results with chromosome 14/22 centromere probe showed that the breakpoint was 14q11 in the patient with corpus callosum agenesis and complex transocation. 2. Determination of breakpoint of inv(16) in acute myelomonocytic leukemia. FISH results with inv(16) probe showed that the breakpoint was between 16pll and p13 in an abnormal inv(16) chromosome. 3. Determination of breakpoint of dup(17q) in acute myelocytic leukemia. FISH results with iso 17q probe showed that the breakpoint was 17q21 and the duplicated segment from q21-q25. 4. Determination of breakpoint of isodicentric X chromosome. FISH results of Turner syndrome with two iso Xq chromosome using a-satellite X centomere probe showed that the breakpoint was in proxim portion of centromere. From the above results, we could easily find out the breakpoints of chromosomal abnormalities by FISH. It was helpful for the tracing of pathogenesis of genetic disease and cancers.
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구선회,권계철,조혜현,성지연 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.5
Background: The emergence of multidrug-resistant (MDR) Acinetobacter baumannii as an important opportunistic pathogen has given rise to significant therapeutic challenges in the treatment of nosocomial infections. In the present study, we assess the antibiotic resistance mechanisms of MDR A. baumannii strains by estimating the prevalence of antibiotic resistance determinants, including integrons, b-lactamases, str genes, and gyrA and parC mutations. Methods: Thirty-five MDR A. baumannii clinical isolates were collected from 3 Korean university hospitals over a 2-yr period. A. baumannii was confirmed by rpoB gene analysis. For each isolate, the minimal inhibitory concentrations (MICs) of 9 antibiotics were determined by the agar dilution method. PCR and DNA sequencing were used to identify the genes that potentially contribute to each resistance phenotype. Results: Of the 35 MDR A. baumannii isolates examined, 7 antibiotic resistance gene determinants were detected. These resistance gene determinants included the gene blaOXA-23, with an upstream element ISAba1 to promote increased gene expression and subsequent resistance to carbapenems, in 8 isolates (22.9%); aacA4, located within class 1 integrons, in 7 isolates (20.0%); and fluoroquinolone resistance conferred by gyrA and parC sense mutations in 31 isolates. Conclusions: Of the 35 MDR A. baumannii isolates, 26 (74.3%) from both outbreak and sporadic cases possessed at least 4 of the 7 antibiotic resistance gene determinants that give rise to the MDR phenotype. The co-occurrence of several resistance determinants may present a significant threat. (Korean J Lab Med 2010;30:498-506)
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구선회 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2
The presence of autoantibody on RBC make diffculties in blood typing and crossmatching. In addition, If alloantibodies exist with autoantibody, transfusion reactions may be brought by masking the alloantibody. So it is important thing to identify the antibody specificity in blood bank working. We analysed 23 cases patients with auto and alloantibodies. The prevalence age with autoantibody were 10th and 50th. Antibodies identified in antiglobulin test were 4 cases of Rh specificities, and 1 case of kell specificity. Another 5 cases with warm antibodies were not identified. The Rh specificities consisted of 2 cases of anti-E, 1 case of anti-e and anti-c, 3 cases were cold antoantibody, bu the specificity were not identified. 6 case were idiopathic origin and other 7 case were secondary to other diseases. 3 cases show severe hemolysis, so transfusion was needed.
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구선회,전영미 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2
With the recent rapid expansion in the use of the comparative genomic hybridization(CGH) technique, increased atttention to improve the quality of technique is essential. In the present study, we performed the optimized condition of CGH technique. 1) The most important procedures were metaphase slide preparation and denaturation. The most appropriate method of slide making was the dropping of the cell suspension onto a slide at 15-30°angle lengthwise and relative humidity adjusted to 60-70% during slide prepartion 2) For preparation of cytoplasm-free metaphase spreads, freshly dropped slide was immersed for 1-2 seconds in the fresh fixative. 3) The most effective denaturation time and temperature was at 75℃ for 2min, after aging at 65℃ for overnight. 4) Duration of hybridization time did not affect the results of hybridization.
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