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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Kang, Hio Chung,Kim, Il-Jin,Park, Jae-Hyun,Shin, Yong,Jang, Sang-Geun,Ahn, Sun-A,Park, Hye-Won,Lim, Sun-Kyung,Oh, Seung Keun,Kim, Dae Jung,Lee, Kwan Woo,Choi, Young-Sik,Park, Young Joo,Lee, Min Ro,Kim National Hellenic Research Foundation 2005 ONCOLOGY REPORTS Vol.14 No.4
<P>Von Hippel-Lindau (VHL) disease is an autosomal dominant inherited tumor syndrome associated with germline mutations in the VHL gene. VHL disease, as well as several other cancer syndromes, has been associated with an increased risk of pheochromocytomas, which are catecholamine-secreting tumors of the adrenal gland. VHL disease genotype-phenotype correlations have been well established based on the type of mutations in the VHL gene. However, although many groups have reported VHL germline mutations in different countries, no previous report has described VHL gene mutations in VHL disease and/or pheochromocytoma patients in Korea. In this study, we used direct sequencing to investigate VHL germline mutations in Korean patients with VHL disease or pheochromocytomas (11 VHL patients and 3 additional members from 7 families, 2 patients from 1 family with familial pheochromocytoma, and 2 cases of isolated pheochromocytoma). We found a total of 7 VHL germline mutations (6 missense and 1 frameshift), 3 of which were novel (323_324delGC, 355T>C and 361G>A). No VHL germline mutation was found in the 2 patients with isolated pheochromocytomas and paragangliomas. This study provides informative data for VHL germline mutations and VHL-related phenotypes in Korea.</P>
Polypyrrole Modified Electrode as a Nitrate Sensor
Sung Chul Kang,Keun-Sun Lee,Jin-Doo Kim,Kang-Jin Kim Korean Chemical Society 1990 Bulletin of the Korean Chemical Society Vol.11 No.2
The potentiometric response behavior of a polypyrrole(PPy) coated Pt electrode to nitrate ion has been studied. The electrode shows a nernstian behavior with a slope of 59 mV over 0.50 M to $1.0{\times}10^{-3}M\;NO_3\;^-$ and a detection limit of $1.0{\times}10^{-4}M\;NO_3\;^-$. The response of the electrode is fast and the selectivities for $I^-,\;ClO_4\;^-,\;and\;IO_4\;^-$ are found to be improved. The effect of pH on the potential response to $NO_3\;^-$ is compared with the existing nitrate ion selective electrodes.
Sensitivities of Entomopathogenic Fungus Aschersonia aleyrodis to Bordeaux Mixtures and Lime Sulfur
Sang Soo Oh,Jeong Heub Song,Soon Yeong Hong,Shin Chan Lee,Seong Keun Kang 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10
Aschersonia aleyrodis was well-known to be a biological control agent for citrus whitefly, Dialeurodes citri. This entomopathogenic fungus is naturally occurred in organic farming citrus orchards in Jeju. Both lime-sulfur and Bordeaux mixtures are extensively used today to control citrus diseases like citrus melanose and citrus scab, especially in organic farming pest management program. The high concentrated lime-sulfur is also used for pest control such as pink citrus rust mite and scale insects. This study was focused to test the conidial germination and sporulation of Aschersonia aleyrodis on potato dextrose agar (PDA) medium containing different concentrations of two fungicides. The conidia of Aschersonia aleyrodis grown on PDA mixed with commercial bordeaux mixtures, CM150-505, at dilution rate 1:200 (water : bordeaux mixture) were well-germinated but not sporulated at all. On the other hand, Aschersonia aleyrodis did not sporulate and germinate on lime sulfur treated PDA medium even at extremely low dilution rate 1:2,048,000 (water : lime sulfur). However, when the mycelial cells grown at 25o C for at least 7 days at soluble starch-tryptone medium were added to PDA, they were well-sporulated even at high dilution rate 1:100 (water : lime sulfur). This result suggested that the spore mixtures of Aschersonia aleyrodis should be applied to field quite long after lime-sulfur spray.
A Numerical Study on the Mechanism of Lee Vortex in the Lee of Large Scale Mountain
Kang Sung-Dae,Kimura Fujio,Lee Hwa-Woon,Kim Yoo-Keun The Korean Environmental Sciences Society 1997 Environmental sciences Vol.1 No.1
Understanding the nonlinear flow caused by orographic effects can be valuable in siting of new businesses, industries, and transportation facilities. In spite of recent work on large-amplitude waves and wave breaking, the studies of flow around large scale mountains have just begun. The generative mechanism of lee vortices in the lee of large scale mountain is investigated by Ertel's theorem. The CSU RAMS is used as a numerical model. According to the numerical results, the isentropes are depressed behind the large scale mountains. This means the vortex lines must run upward and downward along the depression surface because vortex lines adhere to isentropic surfaces. Therefore, the vertically oriented vorticity can be formed in the lee of the large scale mountain. This vorticity plays an important role for orographic precipitation, because strong vertical velocity and cloud bands are developed along isothermal deformation surface.