Development of Sensitive Analytical Method of Rhodanthpyrone A by a LC-MS/MS and its Application to Bioavailability Study in Rats

Kang, Bitna,Yoon, Jeong A,Song, Im-Sook,Han, Young Taek,Choi, Min-Koo Korean Society for Mass Spectrometry 2019 Mass spectrometry letters Vol.10 No.3

A sensitive analytical method of rhodanthpyrone A in rat plasma was developed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). Rhodanthpyrone A and rhodanthpyrone B (internal standard) in rat plasma were extracted by a liquid-liquid extraction method with ethyl acetate. This extraction method gave results in high and reproducible extraction recovery in the range of 73.75-79.90% with no interfering peaks around the peak elution time of rhodanthpyrone A and B. The standard calibration curves for rhodanthpyrone A ranged from 0.5 to 2000 ng/mL were linear with $r^2$ > 0.994 and the inter- and intra-day accuracy and precision and the stability were within acceptance criteria. Using this validated analytical method, pharmacokinetics of rhodanthpyrone A following intravenous and oral administration of rhodanthpyrone A at doses of 2 mg/kg and 30 mg/kg, respectively, were investigated. Rhodanthpyrone A in rat plasma showed multi-exponential elimination pattern with high clearance and volume of distribution values. The absolute oral bioavailability of this compound was calculated as 3.7%. Collectively, the newly developed sensitive LC-MS/MS analytical method of rhodanthpyrone A could be successfully applied to investigate the pharmacokinetic properties of this compound and would be useful for the further studies on the efficacy, toxicity, and biopharmaceutics of rhodanthpyrone A.

Studies on Simultaneous Determination of Chlorophyll a and b, Pheophorbide a, and $\beta-Carotene$ in Chlorella and Spirulina Products

이영자,김소희,김진숙,한정아,서해점,임효정,최수영,Lee Young Ja,Kim So Hee,Kim Jin-Sook,Han Jeong A,Seo Hae Jeom,Lim Hyo Jeong,Choi Soo Young The Korean Society of Food Hygiene and Safety 2005 한국식품위생안전성학회지 Vol.20 No.3

역상 컬럼을 이용하여 건강기능식품 중 클로렐라 및 스피루리나제품에 함유되어 있는 엽록소 a, b,페오포르바이드 a 및 $\beta$-카로틴의 HPLC동시분석법을 확립하였으며, 첨가농도 $50\;\mug/ml$에서 엽록소 a, b, 페오포르바이드 a 및 $\beta$-카로틴에 대한 회수율시험결과, 각각 2.8, 6.0, 10.6 및 $10.4\%$의 상대표준편차와 70.3, 71.6, 60.1 및 $90.5\%$의 회수율을 각각 나타냈다. 이때 검출한계는 $0.1\sim1.0\;\mug/ml$, 정량한계는 $0.2\sim2.0\;\mug/ml$이었으며 검량선 상관계수도 0.995 이상의 직선성을 보여주었다. 국내유통 클로렐라 및 스피루리나제품에 대한 엽록소 a, b,페오포르바이드 a및 $\beta$-카로틴의 함유량을 분석한 결과 엽록소 a $121.g\sim543$, 엽록소 b $0.6\sim160.0$, 페오포르바이드 a 및 P-카로틴 $383.6\sim1713.7mg/ml$ 수준으로 나타났다. 엽록소 b의 함유량은 클로렐라제품에서 평균 374.0 mg/100 g 으로 스피루리나제품의 평균 10.5 mg/100 g 보다 30배 이상 함유하고 있는 것으로 확인되었다. 그러나 $\beta$-카로틴의 함유량은 스피루리나제품이 평균 1335.4 mg/100 g 로 클로렐라제품의 평균 495.0 mg/100 g 보다 평균 함유량에서 2.7배 높은 것으로 나타났다. 국내 건강기능식품공전 중 클로렐라 및 스피루리나제품의 엽록소 a b, 및 페오포르바이드 항목의 규격검사를 본 연구의 동시분석법으로 개정함으로써 각 성분 함량의 정량, 분석시간의 단축 및 비용절감 둥 시험방법을 크게 개선할 수 있을 것으로 기대된다. A simple and sensitive analysis method based on reverse phase (RP) HPLC with UV detector was developed for simultaneous determination of chlorophyll a and b, pheophorbide a and $\beta-Carotene$ in Chlorella and Spirulina products. For added concentration $(50\;\mug/ml)$ of chlorophyll a and b, pheophorbide a and $\beta-Carotene$, recoveries of those were 70.3, 71.6, 60.1 and $90.5\%$, respectively, with relative standard deviations of 2.8,6.0, 10.6 and $10.4\%$. Limit of detection and quantification had ranges of $0.1\sim1.0\;\mug/ml$ and $0.2\sim2.0\;\mug/ml$, respectively. Calibration curve was linear with correlation coefficient of 0.995 for chlorophyll a and b, pheophorbide a and $\beta-Carotene$. Results of simultaneous determination in Chlorella and Spirulina products were showed ranges of $121.g\sim543.0\;\mug/ml$ for chlorophyll a,$0.6\sim160.0\;\mug/ml$ for chlorophyll b, $19.2\sim60.3\;\mug/ml$ for pheophorbide a and $383.6\sim1713.7\;\mug/ml$ for $\beta-Carotene$, respectively. Chlorophyll b contents in Chlorella products were detected above 30 times level to those in Spirulina products. $\beta-Carotene$ contents in Spirulina products were detected 2.7 times level to those in Chlorella products.

DACUM 기법에 의한 병원 임상영양사의 직무기술서 개발

차진아 ( Jin A Cha ),김강은 ( Kang Eun Kim ),김은미 ( Eun Mi Kim ),박미선 ( Mi Sun Park ),박유경 ( Yoo Kyoung Park ),백희준 ( Hee Joon Baek ),이송미 ( Song Mi Lee ),최수경 ( Soo Kyong Choi ),서정숙 ( Jung Sook Seo ) 대한영양사협회 2013 대한영양사협회 학술지 Vol.19 No.3

본 연구에서는 DACUM 기법을 통해 국내 병원 임상영양사의 직무를 조사 및 분석하고 그 결과를 바탕으로 도출된 임상영양사의 직무기술서를 평가함으로써 병원 임상영양사를 위한 직무기술서를 개발하고자 하였다. 2011년 7월초에 DACUM 워크숍을 통해 1차적으로 직무기술서를 도출하고 전문가 집단의 자문회의와 Delphi 조사 방법을 적용한 표적집단 면접을 통해 수정 및 보완 작업을 거쳐 직무기 술서를 도출하였다. DACUM 위원회의 패널로는 3차 의료기관에 종사하고 있는 현직 임상영양사 9인과 임상영양학을 전공하는 교수 4인, 직무분석을 담당하는 교수 1인 등 총 14명이 참여하였다. 전문가 자문단을 별도로 구성하였으며, 의사 1인, 간호사 1인, 임상영양분야 실무자 및 교수 5인이 포함된 전문가의 의견을 추가적으로 수렴하였다. 도출된 직무기술서는 2011년 7∼8월까지 전국 500병상 이상의 종합 병원 90개 중 설문조사에 응답한 46개 병원에서 각각 임상영양 실무책임자 1인으로부터 수집한 자료를 분석하여 현장 적합성을 평가하였다. 본 연구의 결과를 요약하면 다음과 같다. 1. 본 연구에서 DACUM 기법에 의해 처음 도출된 직무는 총 8개의 직무, 33개의 과업, 114개의 과업요소였으나 수정 보완작업을 거쳐 총 7개의 직무, 27개의 과업과 93개의 과업요소로 재분류되었다. 최종적으로 병원 임상영양사의 직무는 영양판정(A), 영양진단(B), 영양중재(C), 영양모니터링ㆍ평가(D), 자문ㆍ협력(E), 영양연구(F), 자기계발(G)로 도출되었다. 2. 각 직무영역별로 세부내용을 보면, (A) 영양판정은 A1. 영양 초기평가하기, A2. 식품영양관련 자료 수집·평가하기, A3. 의학적 자료 점검하기, A4. 신체계측 자료 수집·평가하기, A5. 신체증상 자료 점검하기, A6. 개인력 및 과거력 점검하기, A7. 영양 요구량 결정하기, A8. 영양판정 기록하기의 8개의 과업으로 구분하였다. (B) 영양진단은 B1. 영양진단 도출하기, B2. 우선순위 정하기, B3. 영양진단 기록하기로 나뉘었고, (C) 영양 중재는 C1. 영양중재 계획하기, C2. 영양처방 관리하기, C3. 영양상담하기, C4. 영양교육하기, C5. 영양집중지원하기, C6. 영양중재 기록하기로 구분 하였다. (D) 영양모니터링·평가는 4개의 과업으로 D1. 영양상태 모니터하기, D2. 영양중재과정 모니터하기, D3. 영양중재 결과 평가하기, D4. 영양모니터링·평가 기록하기로 구분하였다. (E) 자문·협력은 E1. 영양자문하기, E2. 협력관계 구축 및 유지하기로 (F) 영양연구는 F1. 질 향상 활동하기, F2. 연구 활동하기로 분류하였다. 마지막으로 (G) 자기계발은 G1. 전문성 향상, G2. 자기관리가 포함되었다. 3. 도출된 임상영양 직무기술서에 대한 평가를 위해 설문조사에 참여한 대상 병원의 유형은 상급종합 병원이 65.2%를 차지하였으며, 종합병원은 34.8%였다. 병원의 재원일수는 8.0±1.5일이었으며 병원 조직에서 영양부서는 6곳을 제외한 39개의 병원에서 진료부서 또는 진료지원부서에 속하였다. 또한 병원에서 영양부서가 단독으로 편제되어 있는 경우가 93%로 높은 비율을 보였다. 병원 임상 영양사의 성별은 여자가 100%로 나타났으며, 연령별 분포는 40∼49세가 가장 높은 비율을 보였다. 근무경력은 10년 이상인 경우가 50% 이상을 차지하였고 최종학력은 석사졸업이 가장 높은 것으로 나타났다. 4. 전체 과업요소의 인지된 중요도를 비교하면 6개의 과업요소를 제외한 대부분의 과업요소들이 4.0점 이상이었고 총 7개 직무영역에 대한 중요도는 ‘영양중재’가 4.6±0.6점으로 가장 중요도가 높게 나타났으며, ‘영양진단’과 ‘영양모니터링·평가’의 직무가 4.5점 이상으로 비교적 높게 조사 되었다. 전체 과업요소 중 수행도가 4.5점 이상으로 매우 자주 수행되는 과업요소가 13개, 4.0점 이상의 자주 수행되는 과업도 29개의 과업요소로 나타났으며, 직무영역 중 ‘영양중재’가 4.2±1.0점으로 높게 나타났다. 직무영역별 난이도를 조사한 결과에서는 ‘영양연구’ 업무가 4.1±0.9점으로 가장 높게 조사되었다. ‘영양진단’과 ‘자문·협력’은 각각 3.8±1.0점, 3.7±0.9점으로 비교적 어렵게 인식되는 직무로 나타났다. 직무영역별 과업요소 전체의 중요도, 수행도, 난이도의 평균은 각각 4.5±0.8점, 3.7±1.3점, 3.5±1.0점으로 나타났으며 유의적인 차이가 나타났다(P<0.001). 5. 임상영양사 업무의 중요도와 수행도 사이의 관계를 나타낸 격자도 분석에서 영양 초기평가하기, 영양요구량 결정하기, 영양판정 기록하기, 영양진단 도출하기, 영양중재 계획하기, 영양처방 관리하기, 영양교육하기, 영양집중지원하기, 영양중재 기록하기, 영양상태 모니터하기, 영양중재 과정 모니터하기는 중요도와 수행도가 모두 높은 영역(doing great)에, 식품영양관련 자료수집·평가하기, 의학적 자료 점검하기, 신체계측 자료수집·평가하기, 신체증상 자료 점검하기, 개인력 및 과거력 자료 점검하기, 영양진단의 우선순위 정하기, 영양진단 기록하기, 영양상담하기, 영양 모니터링·평가 기록하기는 수행도는 높으나 중요도가 낮은 영역(overdone)에 속하였다. 중요도가 높으나 수행도가 낮은 영역(focus here)에는 협력관계 구축 및 유지하기, 질 향상 활동하기가 속하였고, 중요도와 수행도가 모두 낮은 영역(low priority)은 영양중재 평가하기, 영양 자문 활동하기, 연구 활동하기, 전문성 향상하기, 자기관리하기가 속하는 것으로 나타났다. 이상의 결과를 볼 때, 본 연구에서 DACUM 기법에 의해 개발된 병원 임상영양사의 직무기술서는 현장에서 임상영양서비스를 수행하는데 가이드라인으로 사용될 수 있을 것으로 여겨지며, 인지된 중요도가 평균 이상으로 높지만 그에 비해 수행도가 미흡한 과업들에 대하여는 임상영양사에 대한 교육 실시, 업무 분담, 적정인원 배치 등 제도적인 뒷받침을 마련하여 수행도를 높일 수 있는 방안이 강구 되어야 할 것이다. 또한 질병별 맞춤형 임상영양 업무에 관한 후속연구를 통하여 보다 전문적인 환자 영양치료가 가능할 것으로 여겨진다. The present study was conducted to develop a standardized job description for clinical dietitians working in hospitals. A developing curriculum (DACUM) method was used for the job analysis of clinical dietitians. Based on DACUM analysis with 14 members, including clinical dietitians and professors majoring in clinical nutrition and job analysis, information on the duties, tasks, and task elements of clinical dietitians was determined. To verify the job descriptions derived from DACUM analysis, a total of 46 tertiary and general hospitals with over 500 beds were recruited for the survey. The final developed job description for clinical dietitians included 7 duties, 27 tasks, and 93 task elements. The duties consisted of nutritional assessment, nutrition diagnosis, nutrition intervention, nutrition monitoring·evaluation, consultation·cooperation, nutrition research, and self-development. The mean scores of perceived importance, performance, and difficulty on the clinical dietitian`s task elements (out of a maximum score of 5.0) were 4.5, 3.7, and 3.5, respectively, with significant differences between the items (P<0.001). The perceived importance and performance grid of clinical dietitian`s tasks showed that “construction and maintenance of collaboration” (E2) and “activity of quality improvement” (F1) received relatively low scores for performance despite their high importance scores; thus the performance of these tasks requires significant improvement. In conclusion, the job descriptions of clinical dietitians developed from this study are useful for the qualitative improvement of clinical nutrition services in hospitals.

A Study on the Princess Line by Body Types (Part I) - Focused on Body Types of A & H -

Kim, Sook-Jung,Suh, Mi-A The Costume Culture Association 2001 Fashion, industry and education Vol.4 No.3

The main purpose is to study lines on different body types and to disguise any imperfections by using diverse princess lines. We separated festers body shapes into specific body types, A, H by applying both the direct and the indirect measurements. Following are the results of the study: 1. nose are the resulting illusion effects when the shoulder width of the princess line was fixed. In case of the armhole princess line, the illusion that the waist intervals were narrow or wide in A-line silhouette. In case the shoulder princess line, when the waist interval was narrow, it appeared to show narrow shoulder in A-line silhouette. While the wide waist interval shows the wide shoulder width in H-line silhouette. It regards body type H in the same light as H-line silhouette when the waist interval is narrow, it appeared to show narrow shoulder width in A-line silhouette. When the waist interval was wide, it appeared to give the narrow shoulder width. 2. When the princess lines waist width was fixed in order to study illusion effects of waist widths. Generally, for body type A, Whether the waist interval princess line is narrow or wide, the princess line is located comparatively wide, in A-line silhouette appeared to show relatively slender waist. For body type H, whether the waist interval is narrow or wide, the princess line us situated comparatively narrow interval, H-line silhouette appeared to show sum waist but the differences are not significant 3. The illusion of the hip were studied by fixating the width of the skirt and varying the locations of princess line and waist widths. For the location of both armhole and shoulder princess line in H and A-line silhouette, the narrow shoulder width and the waist appeared to show narrow hips. mile they are wide appeared to show wide hips. 4. With both waist and skirt width fuel, all two body types showed taller and slender postures when the princess line originated from the shoulder compare to the armhole.

자아성장 프로그램이 여중생의 자아개념에 미치는 영향

정선아,이정숙 대한간호학회정신간호학회 2001 정신간호학회지 Vol.10 No.3

The aim of the study is to develop self-growth program fitting middle-school girls' developmental stage and by examining what self-growth program influence on self-concept change of middle-school, to provide nursing intervention for the purpose of improving youth' mental health in school study field. Total 155 first grade students, female, at H(experimental group, 75) and W(control group, 80) middle school in K city were sampled for this study for convenience, experimental group were divided into a group of 13~14 students and three counselors proceeded self-growth program by the planned module. This program had been administerd for 5 weeks(1 hour/week) in the morning (May, 15~Jun, 14). While, after one month. The control group was only surveyed two times before and after a five-week period in between. Self-growth program administered in the research was developed by a joint effort of Mental Heath Center in Seo-district, Kwangju Metropolitan city researcher, based on Personality Education Program(Kim, 2000). The self-concept measuring tool is questionaries amended and complemented by researcher using perceptual self (20 items) by Choi Jung hun (1972) and self esteem (10 items) by Rosenberg (1983). Cronbach's α of the this measure is .87. The PC-SAS package program was used to analyze the data along with percentage, χ^2-test, t-test. The results of the study were as follows: 1) hypothesisⅠ; experimental group with educated self-growth program will show more positive change in perceptual self than the control group was accepted. as perceptual self scores of the experimental group turned out to be 64.4 and the control group, 57.6, two group's perceptual self scores show significant difference(t=3.75, p=.000). 2) hypothesisⅡ; experimental group with educated self-growth program will show more positive change in self-growth than the control group was accepted. as self-esteem scores of the experimental group turned out to be 30.6 and the control group. 26.8, two group's self-esteem scores appear significant difference(t=3.75, p=.000). 3) Students' response along the self-growth program include; 'The entering the meeting' influenced on a domain of the faith and self opening, 'Who am I?" influenced on a self-exploration, self-esteem and valuable-valueless, 'I will become this sort of person!' influenced on a self-understanding, self-esteem and capable-incapable, 'Good fellowship' influenced on a self-exploration, self-understanding, self-discovery, and friendly-Isolated and 'The commending a friend' influenced on self-discovery, self-esteem, friendly-Isolated and accepted-rejected positively. This study showed the self-growth program fitting middle-school girl's developmental stage influenced on the self-concept of the middle school girls positively, this program will contribute to positive self concept function in adolescence life. Therefore, it considered necessary to proceed this self-growth program regularly as a part of the school personality education course.

Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation

Byun, Mi Ran,Kim, A Rum,Hwang, Jun-Ha,Sung, Mi Kyung,Lee, Yeon Kyung,Hwang, Buyng Su,Rho, Jung-Rae,Hwang, Eun Sook,Hong, Jeong-Ho Elsevier 2012 FEBS letters Vol.586 No.8

<P><B>Highlights</B></P><P>► Phorbaketal A stimulates osteogenesis. ► Phorbaketal A increases TAZ-mediated osteoblast marker gene expression. ► ERK is an important kinase for phorbaketal A induced osteogenic differentiation. ► Phorbaketal A is suggested as a potential compound for stimulating bone formation.</P> <P><B>Abstract</B></P><P>Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK.</P><P><B>Structured summary of protein interactions</B></P><P><B>TAZ</B> physically interacts with <B>RUNX2</B> by pull down (View interaction)</P>

Effects of Aralia continentalis and Angelica biserrata on Inflammatory Response in Lipopolysaccharide-Induced RAW 264.7 Macrophages and Phorbol Ester-induced Ear Edema

Cheon, Myeong-Sook,Yoon, Tae-Sook,Yasukawa, Ken,Yu, So-Yeon,Kim, Seung-Ju,Choi, Go-Ya,Moon, Byeong-Cheol,Lee, A-Yeong,Choo, Byung-Kil,Kim, Ho-Kyoung The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.2

The roots of both Aralia continentalis and Angelica biserrata, known as 'Dokwhal' in Korea, have been used widely as a traditional oriental medicine to treat inflammation and thrombosis. However, the pharmacological differences between A. continentalis and A. biserrata have not been fully established. In the present study, we investigated and compared the inhibitory effects of 70% ethanolic extracts of A. continentalis (ACE) and A. biserrata (ABE) on the production of inflammatory mediators and secondary swelling from chemically induced ear edema. In RAW264.7 macrophages, both ACE and ABE significantly inhibited the release of nitric oxide, prostaglandin $E_2$, interlukin-lbeta, and tumor necrosis factor-alpha in a dose dependent manner. In addition, the swelling from TPA-induced edema in mouse ears was reduced by ACE and ABE. Overall, ACE showed stronger activities than ABE in vitro and in vivo. Our results indicate that A. continentalis roots possess stronger anti-inflammatory activity than A. biserrata roots.

Effects of Aralia continentalis and Angelica biserrata on Inflammatory Response in Lipopolysaccharide-Induced RAW 264.7 Macrophages and Phorbol Ester-induced Ear Edema

Myeong Sook Cheon,윤태숙,Seung Ju Kim,Goya Choi,문병철,A-Yeong Lee,Byung Kil Choo,Ho Kyoung Kim,So Yeon Yu,Ken Yasukawa 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.2

The roots of both Aralia continentalis and Angelica biserrata, known as ‘Dokwhal’ in Korea, have been used widely as a traditional oriental medicine to treat inflammation and thrombosis. However, the pharmacological differences between A. continentalis and A. biserrata have not been fully established. In the present study, we investigated and compared the inhibitory effects of 70% ethanolic extracts of A. continentalis (ACE) and A. biserrata (ABE) on the production of inflammatory mediators and secondary swelling from chemically induced ear edema. In RAW264.7 macrophages, both ACE and ABE significantly inhibited the release of nitric oxide, prostaglandin E2, interlukin-1beta, and tumor necrosis factor-alpha in a dose dependent manner. In addition, the swelling from TPA-induced edema in mouse ears was reduced by ACE and ABE. Overall, ACE showed stronger activities than ABE in vitro and in vivo. Our results indicate that A. continentalis roots possess stronger anti-inflammatory activity than A. biserrata roots.

Differential expression of soybean SLTI100 gene encoding translation elongation factor 1A by abiotic stresses

Chung, Eun-Sook,Cho, Chang-Woo,So, Hyun-A,Yun, Bo-Hyun,Lee, Jai-Heon The Korean Society of Plant Biotechnology 2009 식물생명공학회지 Vol.36 No.3

The translation elongation factor 1A, eEF1A, catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome by a GTP-dependent mechanism. By subtractive suppression hybridization technique, we have isolated a soybean low-temperature inducible gene, SLTI100 encoding translation elongation factor 1A. Multiple sequence alignments and phylogenic analysis showed that SLTI100 and other eEF1As originated from diverse organisms are highly conserved. RNA expression of SLTI100 was specifically induced by low temperature, high salt, ABA, or drought stress. Based on the subcellular localization of the corresponding gene product fused to GFP, we were able to confirm that SLTI100-GFP was restricted to the nucleus and cytoplasm. We propose that soybean eEF1A may play an important role in translational regulation during abiotic stress responses in plants.

P183 : MicroRNA-1290 mediated cellular mechanism promotes asiatic acid induced apoptosis is in a549 non small cell lung carcinoma cells

( Ho Jung Jung ),( Min Jung Kim ),( Hae Jeong Youn ),( Nam Kyung Roh ),( Soo Young Kim ),( Yu Ri Kim ),( Yu Na Lee ),( Jae Wook Jung ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ),( In Sook A 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Asiatic acid is a putative anticancer agent in several types of cancer cells. Researches of its biological role in negative regulation of cell growth have focused on the extent of induction of apoptosis in a cell-type-specific manner. Objectives: To identify the mechanisms underlying asiatic acid-induced downregulation of BCL2. Methods: MicroRNA microarray analysis was used to investigate the effect of asiatic acid treatment of A549 cells on miRNA expression profiles. We used quantitative RT-PCR to confirm the asiatic acid-induced increase in miR-1290 level with primers specific for the mature miR-1290 sequence. We also assessed the effect of asiatic acid on the levels of BCL2 in A549 cells using western blot analysis. Results: We identified an important regulator of asiatic acid-induced cell death, microRNA (miR)-1290, which sensitizes cells to Asiatic acid-induced cytotoxicity and negatively regulates BCL2 expression. Asiatic acid significantly upregulated miR-1290, and asiatic acid-induced cell death was shown to be dependent on miR-1290 activity. Molecular assays demonstrated that BCL2 mRNA is a direct target of miR-1290-mediated RNA interference. Conclusion: The findings of the present study suggest that miR-1290 suppresses cell viability and cell cycle progression. Our results provide insight into miR-1290-mediated cellular mechanisms in asiatic acid-treated A549 non-small cell lung carcinoma cells.