거대 담낭결석의 내시경적 치료 1예 : 내시경적 유두절개술 후 총수담관으로 하행한 거대 담낭결석 Migrated from the Gallbladder through Cystic Duct after Endoscopic Sphincterotomy

이은우,여향순,임동윤 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

The reason why to migrate a large gallbladder stone into the common bile duct (CBD) remains to be established, but among various reasons the diameter of cystic duct appears to be the most important. None of the gallbladder stones with diameters greater than the cystic duct could be passed through it. However, pre-existing CBD stone increases biliary pressure that consequently dilates the cystic duct system retrogradely, allowing larger stones to migrate into the gut. Furthermore, sphincterotomy may actually prevent formation of gallbladder stone and enhance the removal of pre-existing stones from the gallbladder. We recently have experienced a case of a large CBD stone migrated from the gallbladder through the cystic duct after endoscopic sphincterotomy. The cholangitic symptoms were caused by the stone migrated from gallbladder, but several days later got well. So far any side effects related to this removed stone are not found out since we have successfully extracted a large CBD stone (3.5 x 2cm) with mechanical lithotripsy.

數種動物의 膵組織內 Protein Methylases의 活性度 分布에 관한 硏究

李香雨,韓正煥 成均館大學校 科學技術硏究所 1984 論文集 Vol.35 No.1

Post- translational modification of amino acid residue side-chains in protein has come to be a well known biochemical phenomenon. These reactions include methylation, carboxylation, acetylation, phosphorylation, and hydroxylation. One such side-chain modification of protein, protein methylation is being investigated actively with regard to bacterial chemotaxis, cytochrome c, gene regulation, and carnitine biosynthesis. The methylated amino acids occur in nature in highly specialized proteins. These methylated amino acids are formed by reactions catalyzed by protein methylase Ⅰ, Ⅱ and Ⅲ. In recent studies it was reported that protein carboxymethylation is involved in amylase secretion of parotid gland by isoproterenol. It was also suggested that a small part of the total cellular protein carboxymethylation is directly involved in pancreatic enzyme secretion. On the contrary, other authors reported that there is no relationship between protein carboxymethylation and secretion in pancreas and parotid. In this study we first examined the activities of protein methylase I, II, and III in pancreatic tissues of various animals, such as rat, mouse, guinea pig, rabbit, pig, cow, cat, chicken, and frog. The following results were obtained; 1. The activities of protein methylases were generally high in pancreatic tissues of cat, pig, and rabbit. Specifically, the activity of protein methylase I was very high in pancreatic tissue of cat, but low in the tissue of rat. Bovine pancreas had been shown to be the richest source for protein methylase Ⅱ, while the activity of protein methylase Ⅲ was relatively high in pancreatic tissue of cat. 2. The activities of protein methylase Ⅰ, Ⅱ, and Ⅲ in pancreatic tissue of chicken were generally high. 3. Interestingly, the activities of protein methylase Ⅰ, Ⅱ, and Ⅲ were not detected in pancreatic tissue of frog which is amphibian.

단독주택의 외관형태와 이미지평가에 관한 연구

이청웅,박화수,박길우,이향미 朝鮮大學校 建設技術硏究所 2002 建設技術硏究 Vol.22 No.1

This study analyzes the types of external forms of detached housing buildings, and conducts the S.D test with it and is to structurize the image of external form of buildings. Types of 24 detached housing buildings are analyzed in this vein and the results are as follows: (1) Mass type in the primary cognitive factor of building is mostly simple or multiple box type. (2) As a result of analyzing the secondary cognitive factor of building, horizontal type is most and it shows that it is the favorable structure in the stability of form. Roof shape and side pattern are mostly gable and plat roofs and it is known that traditional gable roof and flat roof influenced by modern construction are shown in housing. (3) To examine the tertiary cognitive factor, surface shape is mostly flat type and it shows a little monotonous composition. Also finishing materials are concrete and brick. S.D test of detached houses doesn't show the extreme psychological test disposition and does somewhat affirmative evaluation. As a result of conducting the factor analysis based on S.D test, nine psychological factors including 「originality」, 「pleasantness」, 「harmony」, 「ornamentation」, 「openness」, 「balance」, 「rhythm」, 「stability」 and 「orientation」. These can be called psychological factor axis which can evaluate the modern detached house.

膵組織 Protein Methylases 活性에 대한 Nicotine의 影響

李香雨,李秀英 成均館大學校 科學技術硏究所 1986 論文集 Vol.37 No.1

It has been known for some time that tobacco smoke or nicotine affects exocrine function of pancreas. Edmund et al. reported that nicotine inhibited pancreatic secretion stimulated by secretin. Furthermore, Solomon et al. have demonstrated that nicotine inhibits the flow of the juice and bicarbonate output. Recently, Majumdar et al. have investigated that the in vitro effect of nicotine on the synthesis and secretion of proteins in dispersed rat pancreatic acini in viro. It was concluded that nicotine stimulated the secretion of preformed zymogen granules and newly sythesized proteins from dispered rat pancreatic acini in vitro. On the other hand, there are many reports that suggest the relationship between protein methylation and pancreatic secretion. Specifically, protein carboxymethylation is involved in arnylase secretion of parotid gland by isoproterenol as well as in pancreatic enzyme secretion. Therefore, in this study we have investigated the effect of nicotine on the activities of protein methylases as well as on pancreatic enzymes in vitro. The following results were obtained; 1) In the presence of 8mM nicotine the secretion of amylase was greatly stimulated from the isolated pancreatic fragments. however, this stimulatory effect of nicotine was inhibited by 2.5mM of cycloheximide. 2) In the case of protein methylase activity, nicotine exhibited the profound stimulation of protein methylase Ⅱ and Ⅲ activity. In the presence of 8mM nicotine, the protein methylase Ⅱ and Ⅲ were stimulated by 3 and 2 folds, respectively, when compared with the corresponding control. This profound increment were blocked by 2.5mM cycloheximide.

췌장염을 일으킨 췌조직내 Protein Methylases 활성도 변화

이향우,김인숙 成均館大學校 科學技術硏究所 1986 論文集 Vol.37 No.2

It has been known for sometime that in the acute pancreatitis the activity of serum amylase is increased in comparision to that of the normal. The increased activity of serum amylase is one of the indications of pancreatitis. The biochemical mechanism of the causes of the disease is not clear yet at the present time. Also, the mechanism of pancreatic secretion is not known. However, there are many reports that suggest the relationship between protein methylation and pancreatic secretion. Strittmatter, et al. reported that in parotid gland ,β-adrenergic agent stimulated the activity of carboxymethylase as well as the secretion of amylase. Povilaitis et, al. reported that CCK-PZ and carbachol exhibited the stimulation of protein carhoxylase activity. In order to examine the relationship between protein methylation and pancreatic secretion, the activities of protein methylase I, Ⅱ, and Ⅲ were determined in the tissues of pancreatitis animals treated with saline, alcohol, and bile juice. The results were as the followings. 1. The serum amylase activity were significantly increased in acute experimental pancreatic rats. 2. In the case of protein methylases, the activities of protein methylase I and Ⅱ were significantly decreased in the tissues of rats induced acute pancreatitis by bile juice and sodium taurocholate. But the activity of protein methylase Ⅲ was not affected by the agents.

폐 내 병변으로 인한 소아 급성 호흡부전의 원인 질환과 예후

이혜영,박지숙,최희라,서지현,김점수,임재영,최명범,박찬후,우향옥,윤희상 대한소아과학회 2004 小兒科 Vol.47 No.5

膵臟 組織내 Protein Methylase I 酵素의 精製

李香雨,李晦榮 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1

An enzyme which methylates the guanido group of arginine residues of protein using S-adenosyl-L-methionine as methyl donor has been designated as protein methylase I. Among the enzyme products of this reaction which thus far have been identified are N^G-mono, N^G,N^c-di-, and N^G, N'^G- dimethylarginine. This enzyme is widely distributed in various organs of rat, being especially rich in pancreas of hog. The level of protein methylase I activity was found to closely parallel the rate of cell proliferation. Elevated activity was observed in fetal tissues, continuously dividing HeLa S-3 cell culture, regenerating adult rat liver, and in fast growing Novikoff hepatoma. Protein merhylase I has been purified from calf brain, rat and calf thymus, Krebs II ascites cells and recently a form of protein merhylase I has also been purified from wheat germ, which is the first report of the enzyme being present in the plant kingdom. In the present paper, protein methylase I has been partially purified from hog pancreatic tissue. 1) Protein methylase I has been partially purified about 14-fold from hog pancreatic tissue with 25% yield. 2) Mainly the enzyme was found in cytosolic fraction of pancreatic cells. 3) Main purification steps were homogenation of the tissue, S_3 fraction of cells, 35% ammonium sulfate ppt., and DIEM-cellulose chromatography.

한국인 여성의 자궁경부암에서 PCR을 이용한 Human papillomavirus 아형의 검출에 관한 연구

김향우,전호종,김윤신,장원재,이미숙,이미자 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The polymerase chain reaction (PCR) was used for detection of human papillomavirus (HPV) in paraffin-embedded tissue. Tissues stored as paraffin blocks were a potential source of DNA for retrospective clinicogenetic analysis. Sections in 6 ㎛ (three or five) thick cut from paraffin blocks of 29 uterine cervix dysplasia, 34 uterine cervix carcinoma in situ, 42 uterine cervix squamous cell carcinoma and 10 uterine cervix metaplasia samples were deparaffinized and incubated at 37℃ for 48hrs. in the lysis solution. Following organic extraction and alcohol precipitation, and extracted DNAs were identified by gel electrophoresis. These DNAs carried out PCR. After repeated cycles of specific oligonucleotideprimed extension of viral DNA with thermophilic DNA polymerase. the type of HPV present was then determined on the basis of the size of the ethidium-bromide stained band visible after agarose gel electrophoresis : for HPV 6 is 263 bp. for HPV 11 is 144 bp, for HPV 16 is 601 bp. and for HPV 18 is 360 bp. This study detected HPV DNA in four cases. which broke down to HPV 16 in two case of uterine cervix carcinoma in situ and two case of uterine cervix squamous cell carcinoma, no HPV type in the remainder. Histologically, HPV types 6, 11 are mainly detected in benign genital warts and lowgrade cervical dysplasias, squamous cell carcinoma frequently contained HPV 16, whereas, HPV 18 was present in adenocarcinoma, adenosquamous cell carcinoma and small cell carcinoma of the cervix. These results indicate that cervical cancers might be clinically influenced by the differences in sutible of the infecting HPV.

시상면 병행에 근거한 신개념의 하악상행지 시상분할 골절단술

백진아,이국엽,오향락,고승오,진우정 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

Sagittal split osteotomy (SSRO) is an indispensable and most popular operation for correction of mandibular deformity. The procedure was considered of bony contact surface and nerve injury in the early stage. The procedure has undergone continuous surgical modifications for condylar displacement, relapse, psychologic analysis, functional & esthetic improvement, fixation method and postoperative evaluation of soft tissue change over time. In this article, we compared the contemporary operation method with new method which was designed through ramal computed tomography. Now we are going to introduce novel conceptual SSRO namely CNU-SSRO designed by Chonbuk National University and suggest the standardization of pre-existed SSRO.

피씨비(PCB) 中毒에 미치는 프라센타(Placenta)의 效果

洪思澳,李香雨,鄭相允 成均館大學校 1983 論文集 Vol.33 No.-

This study was undertaken to investigate the effects of placenta on the toxicity of KC-400 in rabbits. Rabbits were treated with 800㎎ of KC-400 in 2㎖ olive oil via IP injection intermittently day after day for a week and were successively administered with 2㎖ of the placenta extracts via I. V. injection twice a day after day as well as with the placenta extracts with 50 ㎎ of vitamin C as well. The results obtained in this experiment were as follows: 1. When the experimental animals were treated with human placenta, there wasn't any alteration of the hematochemical indicators such as RBC, WBC, Hgb, Het, serum Cholesterol, s-GOT, s-GPT, serum Alkaline Phosphatase and Total lipids in serum. 2. According to the experiment of PCB poisoning, while s-C, s-GPT, s-ALP, and s-TL were increased, RBC, WBC, Hgb and Het were decreased during the experimental periods respectively. 3. The placenta extracts normalized the hematochemical indicators of the PCB poisoned animals after one day treated by the placenta extracts. 4. The combined treatment with the placenta extracts and vitamin C was the same effect as the single placenta treatment only; they were not significant in the t-test. 5. The placenta extracts inhibited the serum tyrosinase activity of the rabbits which was induced by PCB in vivo and in vitro respectively. By the combined treatment with the placenta extracts and vitamin C, the activity of tyrosinase was remarkably inhibited in comparison with the single treatment with the placenta alone.