Effects of clomiphene citrate on ovarian function and embryo developmental capacity in the rat

윤영원,권종국,Yun, Young-won,Kwun, Jong-kuk The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.1

클로미펜 시트레이트가 배란반응, 난자의 형태, 난소 스테로이드 생성 및 수정란 발육에 미치는 영향을 PMSG 처리한 랫드에서 조사하였다. 먼저 세가지 용량(0.05mg, 0.1mg 및 1.0mg)의 클로미펜 시트레이트 또는 부형제를 미성숙의 Sprague Dawley 암컷에 일령 25일부터 27일까지 3일간 투여하였다. 그후 28일령에 이들 모든 암쥐에게 4IU PMSG를, 30일령에는 1.0mg 클로미펜 시트레이트가 처치된 일부의 암쥐에게 10IU hCG를 추가로 투여하였고, 31일령에 모두 도살하였다. 한편 4IU PMSG와 더불어 0.1mg클로미펜 시트레이트 또는 부형제를 투여한 일부의 암쥐는 숫쥐와 교미시킨 다음 임신 2일부터 5일까지 매일 도살하였다. 클로미펜 시트레이트의 용량을 증가시킴에 따라 배란반응(배란율 및 평균 배란난자의 수)과 난소중량이 대조군에 비하여 현저히 감소하였고 반면 배란난자의 변성율(%)은 그 용량에 비례하여 증가하였다. 클로미펜 시트레이트에 의한 배란반응 및 난소중량의 억제적 반응은 10IU hCG 추가투여에 의하여 완전히 대조군 수준으로 회복되었다. 그리고 클로미펜 시트레이트의 투여용량의 증가는 프로제스테론과 안드로젠의 혈장치 감소와 더불어 에스트라디올의 혈장치를 현저하게 증가시켰다. hCG의 추가투여는 이러한 클로미펜 시트레이트 작용에 의해 증가된 에스트라디올치를 현저하게 감소시키고 감소된 프로제스테론치를 증가시키는데 효과적이었다. 0.1mg의 클로미펜 시트레이트를 투여한 임신 랫드로부터 회수한 수정란은 전기간에 걸쳐 그 변성율(%)의 현저한 증가와 아울러 특히 임신 3일부터 그 수가 유의성있게 감소하였다. 클로미펜 시트레이트 투여에 의한 수정란의 난분할 속도도 임신 3일부터 대조군에 비하여 현저하게 지연되었으며 아울러 회수된 수정란의 난분할율(%)도 전기간에 걸처 대조군보다 지속적으로 저하되었다. 위의 결과는 흰쥐에 있어서의 클로미펜 시트레이트 투여에 의한 배란억제반응과 아울러 그 작용기전에 성선자극호르몬의 분비억제 또는 차단작용이 포함됨을 증명하였고 이 약제의 투여에 의한 난자의 형태적 정상성과 수정란발육에 대한 유해효과는 수정이전 시기에 있어서의 난소스테로이드 생성 특히 에스트라디올의 증가에 기인함을 제시한다. The effects of CC the ovulatory response, oocyte normality, ovarian steroidogenesis and subsequent embryo developmental potential were examined in PMSG-treated rats. On Days of 25~27 of age, immature female Sprague Dawley rats were treated with three different doses(0.05, 0.1 or 1.0mg /day) of clomiphene citrate or vehicle. The females subsequently received 4IU PMSG on Day 28 and/or 10IU hCG on Day 30, and were killed on Day 31. Some females given 0.1mg CC or vehicle with 4IU PMSG were then mated and killed on Days 2, 3, 4 and 5 of pregnancy. Compared to vehicle(control) group, by increasing the doses of CC, there were a significant decrease in the ovulatory response as judged by both the proportion of rats ovulating and the mean number of oocytes per rat and a marked reduction of ovarian weight. The increasing doses of CC substantially promoted the degeneration(%) of oocytes ovulating in a dose-dependent manner. The CC-mediated inhibitions of the ovulatory response and ovarian weight were oompletely overcome by a subsequent treatment of hCG. Increasing doses of CC resulted in a siginificant elevation of serum estradiol with the decreased levels of progesterone and androgens. The additive treatment with hCG was effective to reduce the elevation of estradiol and to increase the reduction of progesterone produced by high dose(1.0mg) of CC. The preimplantation embryos recovered from 0.1mg CC-treated pregnant rats demonstrated a progressive early loss from Day 3 of pregnancy with a significant increase in the percentage of degeneration during all periods examined, compared to controls. The rate of progressive embryo cleavage in the CC-treated rats were slower than that in controls from Day 3 of pregnancy. Additionally, the percentage of the cleaved embryos recovered from the CC-treated rats remained significantly lower consistently from Day 2 of pregnancy, compared to control regimen. These results demonstrate a possible mechanism of CC-mediated inhibition of ovulatory response in the rats which may include the attenuation or blockade of the endogenous secretion of gonadotropins and also suggest that its detrimental effects observed on oocyte normality and embryonic development may be caused by abnormal follicular steroidogenesis( especially elevated estradiol) preceding fertilization.

최근 개정된 콜레스테롤 치료 가이드라인에 관하여

윤영원 연세대학교 의과대학 혈관대사연구소 2015 We & You Vol.2 No.1

갑상선 기능항진증 환자에서 치료 전, 후의 혈청 Lp (a) 변화

윤영원,서정건,오세창,남수연,남문석,이은직,김경래,이현철,허갑범 한국지질동맥경화학회 1995 韓國脂質學會誌 Vol.5 No.1

이웃과 사회와 국가를 풍요롭게…

윤영원 새얼문화재단 1996 황해문화 Vol.13 No.-

국내 생활체육 현장의 인력 수급 방향

윤영원 성균관대학교 체육대학 1993 체육대학 학회지 Vol.9 No.1

심혈관 질환 환자의 지방 섭취에 관하여

윤영원 연세대학교 의과대학 혈관대사연구소 2016 We & You Vol.3 No.1

PMSG 전처리한 쥐에 있어서 Antiprogesterone(RU486)과 Antiestrogen(Tamoxifen)이 배란과 Oocyte에 미치는 영향

윤영원,권종국,유규연 韓國受精卵移植學會 1989 한국동물생명공학회지 Vol.4 No.1

The effects of an antiprogesterone (RU 486) and an antiestrogen (tamoxifen) on ovulatory response and oocyte morphology were examined in pregnant mare serum gonadotropin (PMSG)-primed immatare female rats (28 days of age): a comparison has been made on two different regirnens primed with a "control" dose (4 IU) and a "superovulatory" dose (40 IU) of PMSG. Females for control control regimen received three consecutive injections of lmg RU486, lmg tamoxifen, or vehicle at 24, 36 and 48hr, and were killed at 72l'r after PMSG. Animals for superovalatory regimen received lmg RU486, 2.5mg tamoxifen, or vehicle fouowlag the injection schedule comparable to control regimen, and were killed at 60 and 72hr after PMSG. Compared to vehicle group, there was a significant reduction in ovulatory response as judged by the proportion of rats ovulating andi or by the mean number of oocytes per rat for each treatment of RU486 and tamoxifen in both regimens. The activity of tamoxifen in inhibiting the ovulatory response was greater in control, but less in superovulatory regimen than that of RU486 based on the dose employed for each antisteroid. In both regimens, RU 486 did not have any effect 6n the changes in the proportion of degenerate oocytes as well as ovarian weight, well tamoxifen treatment resulted in a marked promotion of oocyte degeneration as well as a great reduction in ovarian weight, compared to each parameter of vehicle group. RU486 treatment in each regimen did not alter the serum levels of any steroid hormones observed. Howerver, tamoxifen treatment was associated with significant increases in serum 17-estradiol and decreases in progesterone in both regimens; also significant increases in androgens in superovulatory regimen. The results illustrate the relative inhibitory activity of RU486 and tamoxifen indicating major steroid hormone involved in PMSG-induced ovulation: 17-estradiol for control and progesterone for superovulatory regimen. It also appears that tamoxifen-associated elevation of circulating 17-estradiol andi or androgens could be in part, a contributing factor to the promotion of oocyte degeneration presumably by producing a hostile oviductal environment after ovulation.ent after ovulation.

혈관의 노화란 무엇인가?

윤영원 연세대학교 의과대학 혈관대사연구소 2015 We & You Vol.2 No.3

study on the efficiency improvement of a passive oil damper using an MR accumulator

윤영원,Seong-Min Lee,박명관 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.11

This study describes the development, modeling, and testing of a hybrid damper for semi-active suspension. The goal of this study is to improve the performance of conventional passive oil dampers using a magneto-rheological (MR) accumulator that consists of a gas accumulator and an MR device. The level of damping is continuously variable by means of control of the applied current in an MR device that is fitted to a floating piston that separates the gas and oil chamber. A small MR device is used to resist the movement of the floating piston. At first, a mathematical model that describes all flows within the damper is formulated and developed in Matlab/Simulink. The MR device is also devised. A mathematical model is adopted to characterize the performance of the device. The formulas derived for the different components of the damper force are combined into a full damper model. Then, the applicability of the MR device to a conventional passive oil damper is tested in a manufactured test environment and evaluated in terms of the damping force vs. the piston velocity. From the results, it is possible to ascertain the MF device’s capability to work as a damper that can supply a variable damping force. Moreover, this research affords a lot of new information about the applicability of MR devices and improvement of the damping force.

심장내과 진료실에서 환자들에게 흔히 듣는 질문들

윤영원 연세대학교 의과대학 혈관대사연구소 2016 We & You Vol.3 No.3