반흔 조직에서의 Superoxide Dismutase 활성도에 관한 연구

조성인(Sung In Cho),서성준(Seong Jun Seo),홍창권(Chang Kwun Hong),노병인(Byung In Ro) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.2

Background : Superoxide disrnutase(SOD) provides a protective defease mechanism against potential cytotoxicity of superoxide radical in the aerobic organism. Although human skin is constantly at risk for developing acute and chronic changes by ultraviolet radiation and phototoxic reactions with exogenous and endogenously procluced photosensitizing molecules, studies in SOD in the human skin are rare. Objective : We measured the level of SOD activities in the scar tissues and the normal human skin specimens. This study was to investigate changes of SOD activity by age, sex, and regional differences of SOD activities in the scar issues and the normal skin. Methods : Aut,hors assayed the level of SOD activit,ies in 32 scar tissues(male 8, female 24) and 11 normal human skin specimens(male 8, female 3), which were obtaine 3 from face/neck(17 and 3 specimens), forearm(only 4 scar tissues), trunk(10 and 8 specimens), and lower extremity(only 1 scar tissue). Results : First, activities of total SOD, Cu, Zn-SOD, and Mn-SOL ere 18.93+5.49, 16.97+55.31, and 1.96+0.90 units/mg proteiii respectively in the scar tissues. Second activities of total SOD, Cu, Zn-SOD, and Mn-SOD were 17.27+7.09, 13.82+6.44, and 3.45+1.07 units/mg protein respectively in the normal skin. Third, the changes of total SOD, Cu, Zn-SOD, and Mn-SOD activities by age and sex were similar each other and three were no significant, differneces between age groups in total, Cu, Zn- SOD, and Mn-SOD activities. Fourth, in sun exposed area and unexposed area there were no significant differences in the scar tissues in SOD activities. But, SOD activite.(total, Cu, Zn, and Mn-SOD) in face/neck were higher than those in trunk and lower extremity in tae normal skin(P<0.05). Conclusion : These findings suggest that there are no differences in the intrinsic SOD activities by age and sex in the mature scar tissues and the normal skin. Differences between exposed and unexposed area in the normal skin are due to the induction of exogenous SOD activity by sun-light generation of superoxide radicals. In wound, increased production of leukocyte derived superoxide radicals is the main factor of increased level of SOD activity. (Kor J Dermatol 1994; 32(2): 277-285)

한국에서 채집된 가지 흰가루병균의 실체

조성은 ( Sung-eun Cho ),최인영 ( In-young Choi ),신현동 ( Hyeon-dong Shin ) 한국균학회 2017 韓國菌學會誌 Vol.45 No.2

한국에서 가지 흰가루병균으로 Erysiphe cichoracearum, Leveillula taurica, Sphaerotheca fusca 모두 3종이 기록되어 있다. E. cichoracearum은 1969년에 기록된 이후 한국에서 발생하는 가지 흰가루병균으로 여긴다. 1998년에는 L. taurica가 가지 뒷면흰가루병균으로 기록 되었으나, 이후에 추가적인 발생기록은 없었다. 2002년에는 S. fusca가 가지 흰가루병균으로 보고되었다. 필자들은 총 22점의 가지 흰가루병균 시료를 채집하여 현미경 관찰 및 염기서열 분석을 실시하였으며, 그 결과 모두 Podosphaera xanthii로 동정하였다. 따라서 한국에서 가지 흰가루병균은 P. xanthii로 표기하는 것이 옳으며, 시설재배에서 드물게 발견되는 L. taurica는 뒷면흰가루병균으로 구별하는 것이 맞다. 반면에, E. cichoracearum (= Golovinomyces cichoracearum)은 가지 흰가루병균으로 표본이 보존되지 않았으며 이후에 채집되지도 않았다. 더구나 가지에서 기록한 E. cichoracearum의 기재는 일반적인 형태적 변이의 폭을 크게 벗어난다. 따라서 E. cichoracearum 흰가루병균의 존재에 대한 과거의 기록은 오류로 생각 된다. 결국 P. xanthii가 한국의 가지에서 발병하는 흰가루병의 주요 병원균으로 판단되며, L. taurica는 드물게 발생되었던 것으로 판단된다. 이 총설에서는 가지 흰가루병균의 역사와 최신 분류체계에 대하여 자세히 기록하였다. Three species of powdery mildew (Erysiphales) on eggplant (Solanum melongena L.) have been listed in Korea, namely Erysiphe cichoracearum (now genus Golovinomyces), Leveillula taurica, and Sphaerotheca fusca (now genus Podosphaera; syn. Podosphaera xanthii). Since E. cichoracearum was recorded on eggplant for the first time in Korea in 1969, it has been regarded as a major powdery mildew agent on that plant. In 1998, the causal agent of powdery mildew on eggplant was recorded as L. taurica, then as S. fusca in 2002. During our extensive field surveys in Korea, we collected 22 samples of eggplant powdery mildews. Our microscopic observations and molecular sequence analyses showed that all of our samples belonged to the genus Podosphaera, in the absence of either E. cichoracearum or L. taurica, suggesting that P. xanthii is the dominant agent of powdery mildew disease on eggplants in Korea. As there have been no additional findings on L. taurica after the first report on the species, it seems to be a minor species that is rarely found in greenhouses. The presence of E. cichoracearum (syn. Golovinomyces cichoracearum s. lat.) on eggplants is questionable, as the morphological characteristics of E. cichoracearum in the original description of the Korean collection deviate from the morphological variations of this species. In addition, no herbarium material of E. cichoracearum remains. Consequently, it seems that P. xanthii is the main species of powdery mildew on eggplants, whereas L. taurica occurs rarely on eggplants, in Korea. This review provides the historical and recent taxonomy of eggplant powdery mildews in detail.

단위가축영양 : 모돈에 대한 생강 및 황금 분말 급여가 초유와 혈청의 항산화력과 IgG 농도에 미치는 영향

김상범 ( Sang Bum Kim ),김인철 ( In Cheul Kim ),송영민 ( Young Min Song ),이성대 ( Sung Dae Lee ),정현정 ( Hyun Jung Jung ),박준철 ( Jun Cheol Park ),조성백 ( Sung Back Cho ) 한국동물자원과학회 ( 구 한국축산학회 ) 2010 한국축산학회지 Vol.52 No.5

본 연구에서는 생강과 황금 건조 분말의 급여가 모돈의 유질과 자돈의 면역력에 미치는 영향을 구명하고자 수행하였다. 공시동물은 2산차 F1 임신모돈 24두를 이용하였다. 시험사료는 대조구(CON) 사료에 생강건조분말(GDP)과 황금건조분말(SDP)를 0.3 % 대체하여 분만 전 30일부터 이유 21일까지 시험사료를 급여하였다. 모돈 혈청에서 항산화력과 IgG는 처리구간에 유의적인 차이를 나타내지 않았다. 초유에서 항산화력은 처리구가 대조구보다 높은 수치를 나타내었지만, 처리구간의 유의적 차이를 나타내지 않았다. 초유의 IgG 함량은 GDP구가 다른 처리구에 비해 유의적으로 높게 나타났다(P<0.05). 초유의 유성분 함량에서, 단백질 함량은 GDP구가 다른 처리구에 비해 유의적으로 높게 함유하였다 (P<0.05) 지방과 유당 함량은 처리구간에 유의적 차이를 나타내지 않았다. 초유의 아미노산 조성은 SDP구가 다른 처리구에 비해 각각의 아미노산 함량이 유의적으로 높게 나타났다. 0일령과 21일령에서 자돈 혈청의 항산화력은 처리구간에 유의적 차이를 나타내지 않았지만, 다른 구에 비해 GDP구가 높은 함량을 유지하였다. IgG 함량은 0일령에서는 측정되지 않았고, 21일령에서는 GDP구가 다른 구에 비해 유의적으로 높은 함량을 나타내었다(P<0.05). 이상의 결과로, 생강 건조분말은 초유의 IgG 함량과 자돈 혈청 IgG 함량을 개선시키는 효과가 확인되어 모돈 사료의 유질개선용으로 활용이 가능할 것으로 판단된다. The objective of this study was to investigate the effects of dietary ginger and Scutellaria on antioxidant capacity and IgG concentration in plasma and colostrum of sows. Twenty-four sows (Landrace x Yorkshire) in parity 2 were assigned to 3 treatment groups (8 sows per treatment) from 30 day before farrowing up to weaning on day 21 after farrowing. The treatment diets included I) CON (Basal diet), 2) GOP (0.3% ginger dry powder was used as the replacement of wheat in basal diet) and 3) SOP (0.3% Scutellaria dry powder was used as the replacement of wheat in basal diet). No differences were found in antioxidant capacity and IgG concentration in plasma of sows among all the three treatments. Antioxidant capacity in colostrum was not affected by replacement of ginger and Scutellaria dry powder. IgG and protein concentration in colostrum was significantly higher (P<0.05) in GOP than that in CON and SOP. However, fat and lactose concentration were not affected by treatments. The total amino acid concentration in colostrum was significantly higher (P<0.05) in GOP than that in CON and SOP. Plasma antioxidant capacity was not affected by treatments in piglet on 0 day and 21 day, but tended to increase by supplementation of ginger. On o day, plasma IgG in piglet was not detected by replacement of ginger and Scutellaria dry powder. However, plasma IgG concentration in piglets on 21 day was significantly higher (P<0.05) in GOP than that in CON and SOP. These results indicate that colostrum IgG concentration in sows and plasma IgG concentration in piglets were improved by feeding ginger dry powder 30 days before farrowing in comparison to basal diet.

배양된 정상 인체 각질형성세포에서 자외선 B 조사에 의한 아포프토시스와 p53의 발현

김명남(Myeung Nam Kim),서성준(Seong Jun Seo),홍창권(Chang Kwun Hong),노병인(Byung In Ro),노성욱(Sung Wook Ro),조성인(Sung In Cho) 대한피부과학회 2000 대한피부과학회지 Vol.38 No.4

N/A Cutaneous absorption of ultraviolet B(UVB) in the skin occurs primarily in keratinocyte, causing DNA and protein damage. p53 tumor suppressor gene appeared in the epidermis after UVB irradiation, and the wild type has been known to be responsible for apoptosis and plays an important role in excluding abnormal cells with significant DNA damage. While p53 has been implicated in both DNA repair and apoptosis, it is unclear whether the p53 protein is involved in both of these processes within the same cell. Therefore, UVB-induced apoptosis and changes in p53 expression were studied in cultured normal human keratinocyte to determine that the cellular response to UVB induced DNA damage(DNA repair or apoptosis) correlated with p53 expression. The cultured normal human keratinocytes were irradiated with the doses of UVB(25-150 mJ/cm2) and incubated for various times(3, 6, 12, 24 hour) after radiation. At UVB doses of 100 and 150 mJ/cm2, acridine orange/ethidium bromide(Ao/Eb) staining-positive cells and TUNEL (TdT mediated dUTP-biotin nick end labeling) staining-positive cells increased significantly after 3 hours and 6 hours postirradiation respectively. Twelve hour postirradiation, staining-positive cells increased at each level of UVB-radiation exposure. These results suggest that there were significant influences of UVB doses and time course after irradiation to the number of Ao/Eb and TUNEL staining-positive cells. To determine whether all Ao/Eb and TUNEL-positive cells were actually undergoing apoptosis, cellular DNA was extracted from keratinocytes at 12 hours after UVB irradiation and seperated by electrophoresis on an 2.5% agarose gel to detect the internucleosomal DNA fragmentation(DNA ladder). 'DNA ladder' occurred at every dose of UVB 12 hour after irradiation, but did not appear early after irradiation, suggesting that whether Ao/Eb and TUNEL-positive cells observed early after irradiation were not undergoing apoptosis. Activation of p53 and the response to DNA damage is not observed universally, but is dependent on tissue specificity, species specificity and type of genotoxic damage. To correlate p53 level with UVB-induced apoptosis at the dose of 100mJ/cm2 UVB, p53 levels were determined by western blot analysis. The accumulation of p53 protein was apparent after 6 hours postirradiation, and UVB irradiation caused a dramatic increase in p53 levels at 12 and 24 hours. These results demonstrate that p53 is required for UVB-induced apoptosis in cultured normal human keratinocyte and p53 has a time-dependent effect in the initiation of apoptosis. In this study, the results indicated that a low dose(25mJ/cm2) of UVB irradiation could induce apoptosis in human keratinocyte in vitro and UVB exerts a time-dependent effect on inducing apoptosis. And the results also give support to increasing evidence that p53 may play a role in UVB-induced DNA damage and the induction of apoptosis in cultured normal human keratinocyte and that p53 is involved in the decision process which determines the fate of keratinocyte after UVB -induced DNA damage. (Korean J Dermatol 2000;38(4):481~489)

조기양막파수 환자에서 산전 Betamethasone 1 회 요법과 다회 요법의 신생아 및 임산부 감염 유병률 비교

황호명(Ho Myong Hwang),장인석(In Seck Jang),정영주(Young Ju Jeong),조성남(Sung Nam Cho) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8

N/A Objective : The optimal management of cervical intraepithelial neoplasia (CIN) after loop electrosurgical excisional procedure (LEEP) remains controversial and reliable predictive factors of residual disease after LEEP have not been consistently identified. This study was performed to identify predictive factors for residual disease after LEEP in patients with CIN. Methods : From June 1996 to May 2000, 166 patients who received subsequent hysterectomy after LEEP according to indication in Dept. of Obstet. And Gynecol. At Chonbuk National University Hospital. The age of patients, the severity of disease, the status of resection margin, and high-risk HPV infection were analyzed for predictive values of residual disease. The student t-test and chi-square test were used for statistical analysis. Results : 1. The residual disease after hysterectomy was negative in 68.1% (113/166) and positive in 31.9% (53/166). 2. The mean age of patients with no residual disease was 45.7 years (range;27-67) and that of patients with residual disease was 49.7 years (range;32-67), showing significant difference (p=0.008). 3. Thirty-three out of 129 cases (25.6%) with negative resection margin and 20 out of 37 cases (54.1%) with positive resection margin in LEEP had residual disease, showing significant difference (p=0.001). 4. Residual disease after hysterectomy was more frequent in patients with more high grade lesions in LEEP, but there was no statistical significant difference (p>0.05). 5. There was no significant difference in the possibility of positive residual disease after hysterectomy between HPV-positive group and HPV-negative group (p=0.84). Conclusion : The negative resection margin in LEEP does not always guarantee that there is no residual disease. More aggressive treatment plan (wide conization or hysterectomy) should be considered in patients who has higher possibility of residual disease such as old age and positive resection margin in LEEP.

착즙부산물을 이용한 발효사료가 육성돈의 영양소 소화율 및 질소균형에 미치는 영향

이제현,정현정,김동운,이성대,김상호,김인철,김인호,오상집,조성백,Lee, Je-Hyun,Jung, Hyun-Jung,Kim, Dong-Woon,Lee, Sung-Dae,Kim, Sang-Ho,Kim, In-Cheul,Kim, In-Ho,Ohh, Sang-Jip,Cho, Sung-Back 한국축산환경학회 2010 축산시설환경학회지 Vol.16 No.1

본 시험은 착즙부산물 발효사료가 육성돈의 영양소 소화율, 분뇨 배설량 그리고 질소 균형에 미치는 영향을 알아보고자 실시하였다. 삼원교잡종 24두(개시체중 46.79 kg)를 공시하여 10일간의 적응기를 거쳐 7 일간 대사 케이지 ($0.5{\times}1.3m$)에서 소화시험을 수행하였다. 처리구는 1) 기초사료 (CON), 2) 발효사료(기초사료를 발효한 사료, F), 3) 케일박 발효사료 (KF), 4) 신선초박 발효사료 (AF), 5) 당근박 발효사료 (CF) 빛 6) 포도박 발효사료(GF)로 하여 총 6개 처리이었으며 처리당 4반복으로 하였다. 시험기간 동안 건물 소화율은 대조구, F, CF 처려구가 다른 처리구들보다 유의적으로 높았다 (p<0.05). 조단백질소화율은 F 처리구가 AF 및 GF 처리구보다 유의적으로 높았고 (p<0.05), 조섬유 소화율은 GF 처리구가 다른 처리구들보다 유의적으로 낮았다 (p<0.05). 조지방 소화율에서는 AF 및 CF 처리구가 KF를 제외한 처리구들보다 유의적으로 높았다 (p<0.05). 조회분 소화율은 CF 처리구가 다른 처리구들보다 유의적으로 높았다 (p<0.05). 반면 칼숨과 인 소화율에서는 CF 및 GF 처리구가 다른 처리구들보다 유의적으로 높았다 (p<0.05). 에너지 소화율에서는 대조구, F 및 CF 처리구가 KF, AF 및 GF 처리구보다 유의적으로 높았다(p<0.05). 총 필수아미노산 소화율에서는 F 처리구가 AF, CF 및 GF 처리구보다 유의적으로 높았고(P<0.05), 비필수아미노산의 평균 소화율에서는 F 처리구가 AF, CF 및 GF 처리구보다 유의적으로 높았다 (p<0.05). 총 아미노산의 평균 소화율에서는 F 처리구가 AF 및 CF 처리구보다 높았으며 (p<0.05), GF 처리구가 가장 낮았다 (p<0.05). 분으로 배설된 질소 비율은 GF 처리구가 다른 처리구들보다 높았고(p<0.05), F 처리구가 낮았다 (p<0.05). 뇨로 배설된 질소 비율에서는 대조구와 GF 처리구가 다른 처리구들보다 유의적으로 낮았다(p<0.05). 질소 축적비율에서는 대조구가 가장 높았으며 (p<0.05), KF 처리구가 가장 낮았다 (p<0.05). 결론적으로 일반배합사료보다 착즙부산물 발효사료의 소화율이 감소하였는데 이것은 사료에 혼합된 착즙부산물 수준 30%가 높은 수준인 것으로 판단되어 추후 섬유소 수준을 낮추어 시험을 수행할 필요가 있다고 생각된다. 특히, 포도박 발효사료 처리구에서 분 배설량이 많은 이유는 포도박 발효사료의 섬유소 함량이 대조구 및 발효사료 보다 높고 다른 착즙부산물에 비하여 소화되지 않는 포도씨가 다량 배설되었기 때문인 것 같다. This study was conducted to evaluate the effects of fermented diets including liquid by-products on nutrient digestibility and nitrogen balance in growing pigs. Treatments were 1) CON (basal diet), 2) F (fermented diet with basal diet), 3) KF (fermented diet with basal diet including 30% kale pomace), 4) AF (fermented diet with basal diet including 30% angelica keiskei pomace), 5) CF (fermented diet with basal diet including 30% carrot pomace) and 6) OF (fermented diet with basal diet including 30% grape pomace). A total of 24 pigs (41.74kg average initial body weight, Landrace $\times$ Yorkshire $\times$ Duroc), were assigned to 6 treatments, 4 replicates and 1 pig per metabolic cage in a randomized complete block (RCB) design. Pigs were housed in $0.5\times1.3m$ metabolic cage in a 17d digestibility trial. During the entire experimental period, Digestibility of dry matter (p<0.05) of treatment CON, F and CF were higher than other treatments. In crude protein digestibility, treatment F was higher than treatment AF and GF (p<0.05). Treatment GF showed the lowest digestibility of crude fiber among all treatments (p<0.05). In ether extract digestibility, treatment AF and CF showed higher than other treatments (p<0.05) except KF treatment. CF treatment showed the best digestibility of ash among all treatments (p<0.05). Whereas, For Ca and P digestibility, CF and OF treatments were improved than other treatments (p<0.05). Energy digestibility (p<0.05) of CON, F and CF treatments were higher than KF, AF and GF treatments. In total essential amino acid digestibility, F treatment was improved than AF, CF and GF treatments (p<0.05). In total non-essential amino acid digestibility, F treatment was higher than CON, AF and GF treatments (p<0.05). In total amino acid digestibility, F treatment was higher than AF and CF treatments (p<0.05) and GF treatment showed the lowest digestibility (p<0.05). In fecal nitrogen excretion ratio, GF treatment was greatest among all treatments (p<0.05) and F treatment was decreased than other treatments (p<0.05). In urinary nitrogen excretion ratio, CON and GF treatments showed the lowest among all treatments (p<0.05). In nitrogen retention ratio, CON treatment showed the high and KF treatment showed the lost among all treatments (p<0.05). Therefore, this experiment suggested that fermented diet could improve nutrient and amino acid digestibilities of growing pigs.

2- Aminoethylphosphonic Acid 를 포함한 광학적활성 Phosphonopeptide 의 합성

조성기(Sung Gi Cho),홍석인(Suk In Hong),김용준(Yong Joon Kim) 한국고분자학회 1980 폴리머 Vol.4 No.6

스펙트럼 센싱을 이용한 주파수 선택구조 제어 시스템 설계

조성실(Sung-Sil Cho),박종환(Jong-Whan Park),이인곤(In-Gon Lee),홍익표(Ic-Pyo Hong) 한국통신학회 2016 한국통신학회 학술대회논문집 Vol.2016 No.1

초등학교 자체평가 모델 개발 : 학교평가 영역 및 지표에 대한 교사와 학부모 집단의 인식 차이를 중심으로

조성범(Sungbum Cho),배상훈(Sang Hoon Bae),장인수(In Su Jang) 학습자중심교과교육학회 2021 학습자중심교과교육연구 Vol.21 No.12

목적 본 연구의 목적은 교사와 학부모의 인식을 토대로 학교 운영의 효율성 제고와 학생 교육의 책무성 확보를 효과적으로 지원하는 초등학교 자체평가 모델을 수립하는 것이다. 방법 이를 위하여 문헌분석과 학교평가 전문가의 검토를 통해 학교평가 이론 모델을 수립하였다. 그리고 도출된 이론 모델의 타당성을 검증하기 위해 오산시 소재 공립 초등학교의 교사 55명과 학부모 39명을 대상으로 각 지표에 대한 중요도와 실행도를 확인하고자 설문조사를 실시하였다. 결과 본 연구 결과, 3개 영역 22개 지표로 구성된 학교평가 모델이 개발되었다. 각 영역별 교사와 학부모가 강조하는 지표가 달랐다. 교사는 학교행정시스템 관련 지표를 중시하는 반면, 학부모는 학생의 안전과 교육 관련 지표를 보다 중시하였다. 결론 본 연구 결과는 향후 공동체적 관점에서 학교를 성공적으로 운영할 수 있는 지표로 활용될 수 있다. Objectives The purpose of this study is to develop self-evaluation model of elementary school that supports to improve the efficiency of the school management and educational accountability based on perceptions on teachers and parent. Methods To this end, a school evaluation theory model was established through literature analysis and reviews by school evaluation expertises. In addition, in order to verify the theoretical model, a questionnaire survey was conducted with 55 teachers and 39 parents of public elementary schools in Osan-si to employ the importance-performance analysis(IPA). Results A school evaluation model consisting of 22 indicators in three areas was developed. teachers and parents emphasized on different indicators each other. Teachers valued indicators related to the school administration system, while parents valued indicators related to student safety and education. Conclusions The results of this study can be used as an indicator to successfully manage schools from a community perspective.

계통주파수 및 전압 저하시 원자력발전소 응동 분석

조성돈(Sung Don Cho),강인수(In Su Kang) 대한전기학회 1999 대한전기학회 학술대회 논문집 Vol.1999 No.7