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정동윤,김동수,이명자,김상록,변대석,김현대,박영호 ( Dong Youn Jeong,Dong Soo Kim,Myung Ja Lee,Sang Rok Kim,Dae Seok Byun,Hyun Dae Kim,Yeung Ho Park ) 한국수산과학회 1994 한국수산과학회지 Vol.27 No.6
Ninety-six specimens of nine puffer fishes landed at a fish market of Pusan, Korea were assayed for anatomical distribution of toxicity. The puffers, Lagocephalus gloveri ($quot;geommilbog$quot;), Fugu rubripes rubripes ($quot;jajubog$quot;), Fugu rubripes chinesis ($quot;geomjajubog$quot;), Lagocephalus wheeleri ($quot;hinmilbog$quot;) and Arothron firmamentum ($quot;byeolbog$quot;), were shown to be nontoxic in muscle and skin. However, toxicities in skins of four specimens of Lagocephalus gloveri and a specimen of Arothron firmamentum were more than 10MU/g In the puffer, Fugu xanthopterus ($quot;ggachibog$quot;), the skin showed to be weakly toxic as far as nine specimens out of 27 ones are concerned, and the muscle was nontoxic in all specimens. Meanwhile, it was noted that the puffer, Fugu vermicularis radiatus was found to be a toxic species, differing from the toxicity known so far. Their highest toxicities were 3,880, 1,191, 1,115, 219, 289, 5,620 and 753MU/g for liver, intestine, skin, muscle, testis, ovary and bile, respectively.
중국으로부터 수입된 삼채복(Fugu flavidus)의 독성
김동수 ( Dong Soo Kim ),김상록 ( Sang Rok Kim ),이명자 ( Myung Ja Lee ),설명훈 ( Myung Hoon Seol ),정동윤 ( Dong Youn Jeong ),김현대 ( Hyun Dae Kim ) 한국수산학회 1995 한국수산과학회지 Vol.28 No.5
본 연구에서는 중국에서 수입되어 시중에서 판매되고 있는 삼채복을 시료로 하여 각 개체의 조직 부위별 독성을 조사하였고 Bio-Gel P-2 칼럼 크로마토크래피로 복어독을 부분정제하여 TLC, 전기영동 및 HPLC에 의하여 독 성분을 검출하고 확인하였다. 삼채복 10개체의 경우 그 독력은 내장, 난소 및 답즙은 ND-l0 MU/g 및 ND-23 MU/g이었으나, 간장, 껍질, 근육 및 정소는 무독하였으며, 유독개제 출현율은 난소(100%). 담즙(20%) 및 내장(10%)순으로 나타났다. 간장부위는 성별에 따른 독력의 차이를 볼 수 없었으나, 생식소의 경우 성별에 따른 차이가 뚜렷하게 나타났다. 한편, 삼채복의 난소로 부터 분리한 독소는 TLC와 전기영동상에서 각각 표준품 TTX와 anh-TTX의 상대이동도와 유사한 두 지점인 Rf 0.64, 0.86와 5.5, 7.2cm에서 나타내어 TTX 관련물질임이 확인되었다. 또한, HPLC분석 결과 TDA, TTX와 anh-TTX의 peak가 뚜렷하게 나타난 반면 4-epi TTX의 peak도 약간 나타났다. Ten specimens of the imported pufferfish, Fugu flavidus ("Samchaebog"), from China were assayed for anatomical distribution of toxicity. Also, a toxic ovary of each specimen was excised, and transferred into Bio-gel P-2 column chromatography for purification of the toxins. The average toxicity of all specimens assayed was calculated to be 4.1±0.5 in liver, 2.6±1.1 intestine, 0.8±0.5 skin, 2.3±1.5 testis, 39.0±16.0 ovary and 7.0±2.0 MU/g bile, respectively; Ovary was weakly toxic, but others were non-toxic or weakly toxic. Moreover, instrumental analyses including thin layer chromatography (TLC) and electrophoresis disclosed tetrodotoxin (TTX) and anhydro tetrodotoxin (anh-TTX), respectively. The toxins of the ovary gave four peaks in high performance liquid chromatography (HPLC) whose retention times (14 and 24 min) were close to those of TTX and anh-TTX, respectively.
조용진,정동윤,최홍대,박종희,손병화,Cho, Yong-Jin,Jeong, Dong-Youn,Choi, Hong-Dae,Park, Jong-Hee,Son, Byeng-Wha 한국생약학회 1998 생약학회지 Vol.29 No.3
Two isoflavonoids were isolated from the flowers of Pueraria lobata (Flos puerariae) guided initially fractionation based on brine shrimp lethality assay. The structures were identified as 4',5,7-trihydroxy-6-methoxyisoflavone (tectorigenin) and 4',7-dihydroxy-6-methoxyisoflavone (glycitein), respectively, on the basis of their spectroscopic and physicochemical evidences.
김동수(Dong-Soo Kim),전선미(Sun-Mi Jhun),정동윤(Sin-Gyu Kang),김현대(Dong-Youn Jeong),박영호(Hyun-Dae Kim) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.3
식용자원의 하나인 고추씨 기름에 관하여 식품화학 및 유화학적인 기초자료를 얻기 위한 연구의 일환으로 고추씨 기름의 triglyceride조성을 분석하고, 중요한 고추 생산지 두 곳을 선정하여 산지별에 따른 그 차이를 비교 검토하였다. 즉 청송산 및 영양산 고추씨 기름의 triglyceride 조성을 밝히기 위하여 시료 유를 TLC에 의하여 triglyceride를 분석하고, HPLC로써 PN별로 분획한 후, 각 획분을 분취하여 GLC로써 acyl carbon number별로 분획하였다. 또한 PN별 획분을 GLC에 의하여 지방산 조성을 분석하였다. 그리고 이들 분석된 결과로 부터 triglyceride조성을 산정하였는데, 청송산 고추씨 기름은 14종류, 영양산은 18종류의 triglyceride로 구성되어 있었으며, 주요triglyceride는 청송산 고추씨기름이 (3×C_(18:0))가 39.6%, (C_(18:0)×C_(18:0))가 37.1%이었고, 영양산의 경우는 (3×C_(18:0))가 41.0%, (C_(18:0)×C_(18:0))가 36.3%이었다. A study was carried out to elucidate the triglyceride compositions of the red pepper seed oils harvested from two different areas. The oil was extracted from the red pepper seed with n-hexane. Each triglyceride of the oil was separated by thin layer chromatography (TLC) and fractionated by reverse phase high performance liquid chromatography (HPLC) on the basis of acyl carbon numbers, and partition number group (PN) and fatty acid composition of triglyceride were analyzed by gas liquid chromatography (GLC). From the results, it was found that the red pepper seed oils of the Chungsong and Youngyang areas consisted of 14 and 18 kinds of triglycerides, respectively. The red pepper seed oil of the Chungsong area consisted of (C_(l8:2), C_(l8:2), C_(l8:2) = 39.6%) and (C_(l6:0), C_(l8:2), C_(l8:2) = 37.1%), and that of the Youngyang area consisted of (C_(l8:2), C_(l8:2), C_(l8:2) = 41.0%), (C_(16:0), C_(l8:2), C_(l8:2) = 36.3%) and (C_(l6:0), C_(l6:0), C_(l8:2) = 8.4%), as the major triglycerides.
일회용 위생장갑에서 Phthalate류 및 Di-(2-ethylhexyl) adipate 분석
이광호,권기성,곽인신,최재천,전대훈,정동윤,최병희,김성욱,이선희,이철원,Lee, Kwang-Ho,Kwon, Ki-Sung,Kwak, In-Shin,Choi, Jae-Chon,Jeon, Dae-Hoon,Jeong, Dong-Youn,Choi, Byung-Hee,Kim, Sung-Wook,Lee, Sun-Hee,Lee, Chul-Won 한국포장학회 2000 한국포장학회지 Vol.6 No.1
일회용 위생장갑에서 diethyl phthalate (DEP), di-n-propyl phthalate (DprP), di-n-butyl phthalate (DBP), di-n-pentyl phthalate (DPP), butylbenzyl phthalate (BBP), di-(2-ethylhexyl) phthalate (DEHP) 및 dicyclohexyl phthalate (DCHP) 등과 같은 phthalate ester 와 di-(2-ethylhexyl) adipate (DEHA) 을 분석하였다. 일회용 위생장갑은 소매점에서 구입하였으며, 사염화탄소를 추출용매로 6시간 동안 Soxhlet 장치에서 추출하였다. 8종의 제품 모두 poly-ethylene (PE) 재질이었으며, 식품위생법에 따라 box 포장에 표기 사항이 있었다. 8 종의 모든 제품에서 Phthalate ester 류 와 DEHA는 검출한계를 넘지 않았다. Phthalate esters 인 DEP, DprP, DBP, DPP, BBP, DEHP, DCHP와 DEHA의 검출한계는 각각 1.8, 1.9, 1.3, 1.1, 0.9, 0.7, 2.1 와 0.8 mg/L 이었다. The level of plasticizer such as diethyl phthalate (DEP), di-n-propyl phthalate (DprP), di-n-butyl phthalate (DBP), di-n-pentyl phthalate (DPP), butylbenzyl phthalate (BBP), di-(2-ethylhexyl) phthalate (DEHP), dicyclohexyl phthalate (DCHP) and di-(2-ethylhexyl) adipate (DEHA) was determined in disposable sanitary gloves. Samples of disposable sanitary gloves were taken at retail shops, and their overall extractions in carbon tetrachloride were measured, after establishment of soxhlet appratus in 6 hours. All of 8 samples of disposable sanitary gloves at retail shops were polyethylene (PE). All of the manufactures was voluntarily labelled their boxes of gloves in line Korea Food Code. The level of phthalate esters and DEHA in all of 8 samples were not exceed the detection limit. The detection limits of DEP, DprP, DBP, DPP, BBP, DEHP, DCHP and DEHA were 1.8, 1.9, 1.3, 1.1, 0.9, 0.7, 2.1 and 0.8 mg/L, respectively.
최홍대,조용잔,손병화,정동윤,박종희 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1
Two isoflavonoids were isolated from the flows of Pueraria lobata (Flos puerariae) guided initially fractionation based on brine shrimp lethality assay. The structures were identified as 4',5,7-trihydroxy-6-methoxyisoflavone (tectorigenin) and 4',7-dihydroxy-6-methoxyisoflavone (glycitein), respectively, on the basis of their spectroscopic and physicochemical evidences.