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변대석,홍정화,권미나,정동윤 한국수산학회 1994 한국수산과학회지 Vol.27 No.2
To evalulate the antioxidant effect of flavonoid(+)-catechin on n-3 polyunsaturated fatty acids in vivo, rats were fed with diets containing 5% corn oil(CO), 5% corn oil and 15% purified fresh fish oil(FO) or peroxidized fish oil(PFO) for 10 days. To accerelate lipid peroxidation, all of them were injected with 60㎎ phenobarbital(a day per ㎏ body weight), and treated with phorone(diisopropylidene acetone) before the rats were killed. Contents of triglyceride, phospholipid, cholesterol and lipid peroxide and the activities of GOT, GPT in serum and total lipid and cholesterol content in liver of PFO group rats were significantly higher than those of the FO one. Contrary to our expectations, the activities of superoxide dismutase(SOD), catalase and glutathione peroxidase(GSH-Px) in liver of the FO group were lower than those of the PFO group. These results might be explained as the results of homeostasis. Even though the hepatic glutathione were depleted, catechin and α-tocopherol inhibited production of lipid peroxide effectively. These results suggested that catechin be considered an antioxidative and hepatoprotective agent.
변대석,홍정화,정동윤,권미나 한국수산학회 1994 한국수산과학회지 Vol.27 No.2
To evaluate the antioxidant effect of flavonoids and α-tocopherol on purified fish oil(up to 40% of n-3 polyunsaturated fatty acids), lipid peroxidation, and fatty acids content dosing storage and upon heating were determined. The potential of these compounds for inhibiting and delaying both oxidation and lipoxygenase processes was also evaluated. The oxidation of fish oil was effectively inhibited by flavonoids and α-tocopherol. The antioxidizing effect of them compounds increased in proportion to their concentration. The addition of α-tocopherol and catechin-α-tocopherol mixture were prolonged induction period of lipid oxidation by 3.5 to 4 times. All other flavonoids also shown more than twice the prolonging effect. Lipoxygenase activity was decreased by catechin and α-tocopherol effectively.