This experiment was conducted to screen the highly susceptible soybean genotypes to Agrobacterium tumefaciens. Plants from 703 lines and 20 cultivars were inoculated with Agrobacteriun tumefaciens Nopaline type strain c58 and Octopine type strain 1595...
This experiment was conducted to screen the highly susceptible soybean genotypes to Agrobacterium tumefaciens. Plants from 703 lines and 20 cultivars were inoculated with Agrobacteriun tumefaciens Nopaline type strain c58 and Octopine type strain 15955. About 97% of tested lines were infected by Agrobacteriun tumefaciens strain c58, which only two lines, B678 and B679 responded to Agrobacteriun tumefaciens strain 15955. The number of highly susceptible lines with higher than 50% tumorigenesis and greater than 1 mm tumor size was 53, while 20 lines were not infected at all. Among the highly susceptible group, the lines 53, 79, 463, 551, 674, 675, 677, 678, and 679 could be used for transformation studies due to their high tuomrigenesis and large tumor size. The lines selected in the field test according to the degree of susceptibility showed the same responses to Agrobacterium infection in vitro.