밤바구미(Curculio dentipes Roelofs)의 유충.용의 혈단백질에 관한 연구

이경로,신병식 한국곤충학회 1988 Korean journal of entomology Vol.18 No.2

밤바구미의 각 시기에 따른 혈단백질 패턴과 함량을 SDS-polyacrylamide gel electrophoresis법과 spectrophotometer를 사용하여 분리측정하였다. 1. 혈단백질 함량은 월동전 유충에서 최고의 함량을 나타내었다. 월동중 감소하다가 월동후 다시 증가하였으며 종령유충기부터 계속 감소하여 응시기에 최저 함량을 나타냈다. 2. 혈단백질 패턴은 전시기에 걸쳐 최고 230,200 daltons에서 최저 15,900 daltons까지 모두 44개의 밴드가 나타났으며 가장 염색강도가 강한 밴드는 분자량 77,900 doltons으로 모든 시기에서 나타났다. 특히 용시기에서 단백질의 변화가 크게 나타났다 The pattrens and concentration of hemolymph protein in the chestnut weevil, Curculio dentipes (Roelofs) according to development stages were determined by SDS-polyacrylamide gel electrophoresis and spectrophotometric method. The protein contents were found to be the maximum at the nonoverwintering larva and decreased at the wintering larva and the overwintering larva and then reached minimum level at pupa stages. The denatured proteins were separated into 44 bands between the highest M.W. 230,200 daltons and lowest M.W. 15,900 daltons during all stages. The band of M.W. 77,900 daltons appeared during all stages and showed greatest color density. Particulary, at overwintering larva and pupal stages, large variation were observed in protein bands.

한출변화과정(汗出變化過程)을 통(通)한 태음인(太陰人) 만성해수(慢性咳嗽) 치험례(治驗例)

이경로,이상기,송정모,Lee, Kyung-Ro,Lee, Sang-Gi,Song, Jung-Mo 사상체질의학회 2004 사상체질의학회지 Vol.16 No.3

1. Objectives In this study, it is experienced that the process of sweating is important in chronic cough of Taeumin. In this case report, the patient has been simproved throuth reaction of sweating improvement. In "Dongyi Soose Bowon", The process of sweating change is described in detail. "Hamkeylhae" is similar to chronic cough in "Dongyi Soose Bowon", it is cough that is hard to spit. The chronic cough of Taeumin is related to the pathology of Liver and Lung, sweating condition is also related to condition of Lung in Sasang constitutional medicine. So the study for the process of sweating is needed. 2. Methods One patient diagnosed as chronic cough was practiced with Sasang constitutional medicine. And We observed sweating change process during treatment. 3. Results As sweating condition improved, chronic cough also decreased, but not improved, not decreased. 4. Conclusions So treating of Taeumin's chronic cough is closely connected with sweating change process.

한국산 밤나무흑벌의 변태에 따른 지방산에 관한 연구

이경로 한국통합생물학회 1965 동물학회지 Vol.8 No.2

Fatty acid components of the Chestnut gall wasp (Dryocosmus kuriphilus YASUMATSU) were analyzed by the method of gas chromatogrphy at various developmental stages. 1. The fatty acids identified at each stage are as follows : Larva stage : acetic acid , benzoic acid and capric acid. Prepupa stage : acetic acid, benzoic acid and capric acid. Pupa stage : acetic acid, benzoic acid and capric acid. 2. Through the development of the wasp, there were large amount of acetic acid.

한국산 화랑곡 나방의 유리 아미노산에 관한 연구

이경로 한국통합생물학회 1964 동물학회지 Vol.7 No.1

The free amino acid content of Indian meal moth (Plodia interpunctella HUBNER) was analysed at various developmental stages by means of paper chromatography. 1) The free amino acids : present are alanine , arginine, aspartic acid, glutamic acid, glycine, histidine, leucine, methionine, proline, serine, threonine, tyrosine and valine. 2) Proline was detectable only in the acid-hydrolyzed Indian meal moth. 3) Arginine was clearly detected only in the larva stage. 4) Tyrosine methionine and valine were increased in the pupa stage. 5) Serine, glycine and tyrosine were present in high concentration in all stages.

솔잎혹파리 幼蟲의 血淋파 變化

李敬魯,李鍾鎭 건국대학교 1977 論文集 Vol.5 No.1

The concentration of free amino acids, total protein, trehalose, lipid, acid·alkaline phosphatase and glutamic oxaloacetic transaminase and glutamic pyruvic transaminase during the larval stage of the pine leaf gall midge, Thecodiploses japonensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Anthrone method, Thin layer chromatographic method, Bessey-Lowry method and Reitman-Franked method, respectively. Healthy specimens were chosen as samples of each developmental stages : the larve in gall, larva in soil and over wintered larva. Amino acids presentin the alcoholic extracts were alanine, aspartic acid, glutamic acid, glycine, histidine, methionine, proline, serine, threonine, tryptophan and valine. The total protein concentration reached to 195.925 mg/g during the larva in gall and the larval stage in soil of the value was decreased to 181.419 mg/g. In the over wintered larval stage the concentration reached a maximum that was value to 224.200 mg/g. The hemolymph sugar, trehalose value for over wintered larva was about two times the value for larva in gall. Total lipid, phospholipid, monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at each stages. Triacylglycerol concentration reached high level in contrast with other lipid contents during the larva in gall, larva in soil and over wintered larva. Monoacylglycerol, sterol and free fatty acid expert decal·eased lipid contents during at each stages. The activity of acid phosphatase generally decreased at larva in soil and increased at the over wintered larval stage. And in the throughout all stages there significant differences between both control and treatments group of acid allkaline phosphatase. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase decreased gradually from larva in gall until the larva in soil and increased at over wintered larva stage. Hence, changes in free amino acids, total protein, acid phosphatase, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase through the course of the physiological cycle form a U-shaped curve when graphed against the chemical composition and activity of hemolymph during the larva in gall, larva in soil and over wintered larva.

먼지시료채취 숙련도 시험 점수 분포에 따른 시험소별 부적합 사례 분석

이경로,고상호,김지혜,전수아,이진주,이혜리,황종연,최종우 환경독성보건학회 2019 한국독성학회 심포지움 및 학술발표회 Vol.2019 No.10

매미나방(Lymantria dispar)의 유약호르몬과 유약호르몬 에스테라제의 활성도

이경로,안기흥,이충언,강정호,신병식 한국곤충학회 1995 Entomological Research Vol.25 No.4

매미나방(Lymantria dispar) 유충의 혈림프 유약호르몬(JH)을 영(instar), 일(day), 시간(hr)에 따라 암수 구분하여 Galleria 큐티클 왁스 검사와 Gas liquid chromatography(GLC)로 조사하였고, 이를 조절하는 유약호르몬 특이 에스테라제(JHE)의 활성도를 측정하여 비교하였다. Gallrria 큐티클 검사 결과 JH titer는 암수 모두 4령(IV)에서 가장 높은 활성을 나타내었으며, 수컷은 5령(V)에서 17,000GU/g 암컷은 6령(VI)에서 6,000GU/g으로 최저의 JH titer를 나타내었다. JH titer에서 최고치를 보인 4령 유충의 1일부터 7일까지 일별 JH titer의 변화는 수컷은 4령 2일에서 암컷은 4령 1일에서 가장 높게 나타났다. 일별 JH titer에서 최고의 활성을 나타낸 4령 2일(♂), 1일(♀)의 시간별 JH titer는 암수 모두 12시간에 서 55,000GU/g(♂), 48,000GU/g(♀)으로 가장 높은 활성을 나타내었다. GLC로 분리한 결과 5가지 측정되었으나 JH I, II, III 3종류는 동정되었고, 2가지는 미동정되었다. GLC에 의한 JH titer의 변화는 전체적으로 4령 유충에서 가장 높은 활성을 나타낸 후 감소하여 6령에서는 아주 미미한 양이 검출되었다. 성별 JH titer의 변화는 전체적으로 암컷이 수컷보다 높은 값을 나타냈다. 동정된 JH별 변화에서는 영과 성에 관계없이 JH II가 가장 높은 titer를 나타낸 반면 JH I은 4령에서 0.029ng/m1(♂), 0.0515ng/ml(♀)로 나타났고, JH III는 특이하게 수컷 4령에서 0.0283ng/m1로 낮은 titer를 보였다. 유충의 각 시기별 JHE의 활성도는 2령 수컷에서 0.8n mole/min/ml로 최저이고 5령에서 3.6n mole/min/ml로 최고값을 나타냈다. JH의 활성이 가장 높은 4령의 날짜별 JH 가수분해율은 수컷의 5일에서 최저이고 7일에서 최고인 반면 암컷에서는 1일에서 최저이고 4일에서 최고치를 나타내어 전반적으로 수컷이 더 높은 활성을 나타내었다. JH titer가 가장 높은 4령 2일(♂)과 1일(♀)의 시간별 JH 가수분해율은 수컷에서는 6시간에서 2.3n mole/min/ml의 높은 JHE 활성도를 나타내었고, 암컷에서는 24시간에서 4.7n mole/min/ml로 최고치를 나타내어 역상을 나타내었다. Haemolymph juvenile hormone(JH) titers were examined using Galleria cuticle wax test and gas liquid chromatography(GLC) and juvenile homone specific esterase(JHE) activities, which control JH, measured in both sexes of the gypsy moth, Lymantria dispar, during different larval stages, days and time. Galleria cuticle wax test had the highest JH titer at the 4th instar larvae on both sex(30,000 GU/g(♂), 33.000 GU/g( ♀)), and the lowest JH titer at the 5th instar larvae in male(17,000 GU/g), 6th instar larvae in female(6,000 GU/g). When JH titer was measured from day-1 to day-7 at the 4th instar larvae, it was high at day-2 in male(42,000 GU/g) and day-1 in female(37,000 GU/g), When JH titer was measured according to time, its high level was observed at 12 hour on both sexes(55,000 GU/g(♂) and 48,000(♀)). We found five JHs and three of them were identified as JH I, II, III, but two of them unidentified. 4th instar larvae showed a dramatic change of JH titers and female was me-re extreme than male. JH IIshowed the greatest change of its level regardless of different stages of larvae and sexes. JH I showed 0.029ng/m1( ♂ ), 0.015ng/ml(♀) at 4th instar larvae and specially JH III 0.0238 ng/ml at male 4th instar larvae, which is lower titer. Juvenile hormone specific esterase(JHE) activities of the gypsy moth in the different larval stages were lower at the 2nd instar larvae in male(0.8 mole/min/ml), and highest at the 5th instar larvae(3.6n mole/min/ml). When hydrolysis rate of JH at the 4th instar larvae whose JH titer was highest was measured, in male larvae, it was lowest(0.9n mole/min/ml) at day-5 and highest at day 7, and in female larvae, it was lowest(0.9m mole/min/ml) at day-1 and highest(3.3n mole/ min/ml) at day-4. Hydrolysis rate of JH in day-2(♂) and day-1 at the 4th instar larvae, whose JH titer was highest, was high in JHE activity at 6 hour(2.3 mole/min/ml) in male and 24 hour(4.7n mole/min/ml) in female. In conclusion, JH titer and JHE activity was shown to be inversely proportional.

파노라마 이미지 기반 실내 장소 인식 실험 데이터세트 구축 및 검증 방법

이경로,이종훈 한국멀티미디어학회 2023 멀티미디어학회논문지 Vol.26 No.12

As interest increases in robotics, virtual reality, and indoor navigation systems, indoor location information extraction systems are becoming important. Among them, visual place recognition technology is being actively researched. Existing methods mainly use monocular images outdoors to recognize places. However, indoor monocular images alone provide insufficient information. Therefore, in this study, we build an indoor place recognition experimental data set based on panoramic images containing more information and propose a verification method. The proposed method showed better place recognition results when using panoramic images than monocular images. Therefore, this proposed method is expected to be helpful in developing an indoor location information extraction system and can be used in various fields.

솔잎혹파리의 변태에 따른 용기의 체액분석

이경로,이종진,이상석,고진복 한국곤충학회 1982 Korean journal of entomology Vol.12 No.2

The concetration of total protein and non protein nitrogen, lipid, trehalose, acid$.$alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and mineral salts during the pupal stage in the pine leaf gall midge, Thecodiptosis japonensis Uchida et Inouye, were measured by using micro-Kjeldahl method, Thin layer chromatographic method, Anthrone method, Reitman-Frankel method, Oser method and EEL dame photometric method respectively. Healthy specimens were chosen as samples of each developmental stages; the prepupa and the late pupa. The concentration of total protein was 88.139mg/g in the prepupa and was 120.719mg/g in the late pupal stage. Non protein nitrogen contents was 2.292mg/g in the prepupa add was 4.225mg/g in the late pupal stage. In the late pupal stage total protein and non protein nit-rogen contents was higher than those in the prepupal stage. Total lipid, phospholipid, menoacylgly cerol, free fatty acid, sterol and ester cholesterol were identified in the prepupa and the late pupal stage. The concentration of total lipid was 150.0mg/g in the prepupa and was 162.8mg/g in the late pupal stage. With the exception of total lipid, phospholipid, monoacylglycerol, free fatty acid, sterol and ester cholesterol contents were higher than in the late pupa those in the prepupal stage. Rf value of trehalose analyzed on thin layer chromatogram was 0.714 in both stages, and the concentration of trehalose was 3.640mg/g in the Prepupa and was 3.553mg/g in the late pupal stage. The activity of acid phosphatase was 0.272unit/mg in the prepupal and was 0.281unit/mg in the late pupal stage, and alkaline phosphatase was 0.413unit/mg in the prepupa and was 0.546unit/mg in the late pupal stage. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase showed the same patterns wi th acid . alkaline phosphatase. Mineral salts present were calcium, phosphorus, sodium and potassium, which were identified in both stages. All kinds of mineral salts contents in the late pupa were higher than those in the prepupal stage. In view of controlling pest, the patterns of the physiological activties indicate that control will be the most effective at the time of the worst physiological manifested at the prepupal stage. 솔잎혹파리 (Thecodiplosis japonensis Uchida et Inouye)의 변태기 중 전용과 후용의 액체 변화를 분석하고 그 활성을 측정코자 micro-Kjeldahl법, Thin layer chromatography법, Anthrone법, Reitman-Fra-nkel법, Oser법, EEL flame photometer법으로 측정분석하였다. 1)전용과 후용에서 총단백질과 비단백성질소, 지질, 탄수화물, phosphatase, GOT, GPT, 무기염류의 함량과 화성도가 측정되었다. 2) 총단백질과 비단백성질소 모두 후용에서 높은 함량의 변화를 나타냈다. 3) 분석된 지질은 총지질, 인지질, monoacylalycerol, 유리지방산, sterol, triacylglycerol, ester cholesterol이였으며 triacylglycerol을 제외하고 후용에서 각 지질의 함량이 전용에서 보다 높았다. 4) trehalose의 함량은 큰 차이는 없었으나 전용에서 다소 높았다. 5) 효소 활성도의 변화는 acid.alkaline phosphatase, GOT, GPT 모두 후용에서 높았으며, GOT는 GPT에 비해 매우 높은 활성을 보였다. 6) 무기염류는 칼슘, 인, 나트륨, 칼륨이 분석되었으며, 칼륨이 가장 높은 함량을 나타냈다. 7) 전용과 후용의 생리적 활성을 비교하면 전용기가 생리적 기능하강기로 나타나 이 시기가 생물학적 방제의 최적기로 이용될 수 있을 것이다.

코로나 시대의 비대면 사역의 방안과 전망

이경로 한신대학교 신학대학원 2020 세계와 선교 Vol.- No.234