발효홍차가 흰쥐의 혈전증과 혈액성분에 미치는 영향

고진복 신라대학교 자연과학연구소 1997 自然科學論文集 Vol.3 No.-

발효홍차를 음료수에 40% 수준으로 혼합하여 성숙된 흰쥐에 3주간 급여한 후, endotoxin으로 혈전증을 유발시켜 혈전 혈성에 관여하는 인자와, 혈청과 간의 지질농도 및 단백질 농도에 미치는 영향을 조사하였다. 체중과 간중량은 각 실험군이 차이를 보이지 않았다. 혈장의 prothrombin time, activated partial thromboplastin time 및 thrombin time은 정상대조군과 발효홍차군(TF)이 비슷하였으나, endotoxin을 처리한 대조군(CE)이나 발효홍차군(TFE)이 정상 대조군에 비하여 유의하게 연장되었다. 혈청의 총 지질, 중성지질 및 인지질의 농도는 정상대조군과 발효 홍차군은 비슷하였으나, endotoxin을 처리한 CE군과 TFE군이 대조군에 비하여 유의하게 증가되었다. 혈청의 총 콜레스테롤 농도는 각 실험군이 변화를 보이지 않았으나 HDL-콜레스테롤 농도는 대조군에 비하여 CE군과 TFE군이 유의하게 감소되었다. 간의 중성지질, 인지질 및 총 콜레스테롤 농도는 모든 실험군에서 비슷한 경향을 보였다. 혈청의 단백질 농도는 대조군에 비하여 CE군과 TFE군이 유의하게 감소되었고, 알부민농도는 TFE군이 감소되었다. 간의 단백질 농도는 유의성 있는 변화는 보이지 않았다. This study was performed to investigate the effects of tea fungus on plasma clotting factors, serum lipid and protein concentrations of thrombosis versus non-thrombosis rats. The male rats (Sprague-Dawley, 25-week-old) were fed 40% tea fungus mixed with their dringking water or without tea fungus for 3 weeks, after the thrombosis was induced by injection of endotoxin into the caudal vein of rats. The rats were divided into 4 groups : control group, control group treated with endotoxin(CE), 40% tea fungus group(TF) and TF group treated with endotoxin(TFE). Prothrombin time, activated thromboplastin time and thrombin time were significantly elongated in the CE and TFE groups compared with those of the TF and control groups, but those were not affected by the tea fungus feeding. Serum total lipid, triglyceride and phospholipid concentration were significantly increased in the endotoxin-treated (CE and TFE) groups compared with those of the TF and control groups. Serum total cholesterol, liver lipids concentration of the CE, TF and TFE groups were similar to those of the control groups. Serum HDL-cholesterol concentration, HDL-cholesterol per total cholesterol ratio and total protein concentration in the CE and TFE groups were much lower than those in the TF and control groups. The results indicated that serum total lipid, triglyceride and phospholipid concentration were significantly increased, and HDL-cholesterol, HDL-cholesterol per total cholesterol ratio and total protein concentration were decreased after inducing thrombosis by endotoxin.

발효홍차 음료가 암쥐의 성장률, 단백질, 지질대사 및 효소활성에 미치는 영향

고진복,노민희 신라대학교 자연과학연구소 2002 自然科學論文集 Vol.10 No.-

This study investigated the effect of tea fungus/kombucha beverage (TF) on the body weight gain, protein levels, lipid metabolism and enzyme activities in growing female rats. The rats (Sprague-Dawley) were divided into three groups; control (TP free water), 20 or 40% TF groups (20 or 40% TF in water) according to the levels of TF supplementation. The rats were fed ad libitum each of the experimental beverage for 7 weeks. The body weight gain, the liver and pancreas weights, the hemoglobin concentration and hematocrit value, and the serum total lipid, triglyceride, total cholesterol and phospholipid concentrations, HDL-cholesterol/total cholesterol ratio, and the serum total protein and albumin levels of the 20 or 40% TF groups were similar to those of the control group. The serum glucose and liver phospholipid concentrations of 40% TF group were significantly increased compared with those of the control group. The serum HDL-cholesterol concentration of the 20% TF group was significantly higher than that of the control group, but the seam LDL-cholesterol concentration and the atherogenic index were significantly lower. The liver cholesterol concentration of the 40% TF group and the liver triglyceride concentration of the 20 or 40% TF groups were significantly lower than those in the control group. The liver total protein level of the 40% TF group was more significantly decreased than that of the control group. The serum GPT activity of the 20 or 40% TF groups were significantly lower than that of the control group. These results indicated that the serum LDL-cholesterol and liver triglyceride concentrations, and the atherogenic index of the female rats fed 20% tea fungus/kombucha beverage were decreased, but the seam HDL-cholesterol concentration was increased. There were no differences in the growth, organ weight, serum protein, triglyceride and total cholesterol concentrations, GPT and ALP activities

발효홍차 음료가 콜레스테롤을 급여한 흰쥐의 지질대사 및 효소활성에 미치는 영향

고진복 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.12 No.-

The effects of tea fungus/kombucha beverage (TF) on the body weight gain, lipid metabolism, protein levels, and enzyme activities in growing male rats fed high cholesterol diet were studied. Sprague-Dawley rats were received 4 types of diets for 4 weeks, respectively: a control diet group, a control TF diet group (control diet + 20% TF in water), a cholesterol diet group (control diet + 1% cholesterol) and a cholesterol TF diet group (cholesterol diet + 20% TF in water). The body weight gains, food intake, food efficiency ratios, and kidney and spleen weights of the rats fed all experimental diets were similar to those of the rats fed control diet. There were no differences in the concentrations of triglyceride, HDL-cholesterol, total protein, and glucose, and the activities of GOT, GPT, LDH and alkaline phosphatase in the serum among the experimental diet groups. The hepatic weights and the concentrations of hepatic total lipid, cholesterol and triglyceride of the rats fed cholesterol diet and cholesterol TF diet were significantly higher than those of the rats fed control diet. The concentration of L DL-cholesterol and HDL-cholesterol/total cholesterol ratio in the serum of the rats fed cholesterol TF diet were similar to those of the rats fed cholesterol diet. The concentrations of total lipid, total cholesterol and free cholesterol, and atherogenic index in serum of the rats fed cholesterol TF diet were significantly decreased compared with the rats fed the cholesterol diet. In conclusion, the 20% tea fungus/kombucha beverage feeding decreased the concentrations of the total lipid, total cholesterol and free cholesterol, and the atherogenic index in the serum of the rats fed high cholesterol diets, but, it could not decrease the concentrations of the total lipid, cholesterol and triglyceride in the liver.

이화명충의 變態에 따른 生理學的 硏究

高鎭福 건국대학교 1979 論文集 Vol.9 No.1

The concentration of total nitrogen and non-protein nitrogen, whole body protein patterns, trehalose, lipids and the activities of acid · alkaline phosphatase, glutamic pyruvic transaminase, glutamic oxalcacetic transaminase and succinic acid dehydrcgenase, mineral salts arid nucleic acid during the metamorphcsis of the rice stem bores, Chilo suppressalis Walker was measured using micro-Kjeldahl method, polyacrylamide gel electrophoresis method, gas chromatographic method, thin layer chromatographic method, Warburg's manometric method, King's method, Reitman's method and Thunberg's method, Schneider methods. Healthy specimens were chosen as samples of each developmental stages: a final instar larva, a newly molted pupa, pupae at 2,4,6,8 and 10 days, newly emerged adult and adults. The total nitrogen concentration decreased gradually until the two day pura stage was reached and increased from the four day pupa. In the final instar larval stage, the newly emerged adult and two day adult stage the concentration reached a maximum. The non·protein nitrogen concentration increased gradually until the newly molted pupa stage was reached and decreased from the two day pupa. The protein bands were numbered 1~14 according to their mobilities. A total of 14 protein patterns were detectable on the electrophotograms of whole body homogenates. Each develop mental stage shows qualitative differences in their protein bands. In general, the protein bands increased with the growth of larva, decreased at the newly molted pupal stage, and at the pupal and adults stages increased gradually again. The hemolymph trehalose concentration decreased gradually until the four day pupa stage and increased from the six day pupa. In the last instar larval arid the newly emerged adult stage the concentration reached a maximum. Phospholipid, monoacylglycerol, diacylglyrerol, triacylglycerol, free cholesterol and ester cholesterol were identified at each stage. Triacylgycerol at the final instar larva the concentration leached a maximum. Triacylycerol decreased gradually at each of the following stages prepupa, two day pupa, four and six day pupa and increased at the eight day pupal stage. The nucleic acid concentration decreased at the last instar larval stage and increased to reach a maximum at the adult stage. The mineral salts present are calcium, phosphorus, potassium, sodium and lithium which are identified at each stage. The activities of acid phosphatase generally increased until the two day pupa stage and decreased at the four day pupal stage, and they increased again at the eight day pupa and the newly emerged adult stages. The activity of alkaline phosphatase is by far lower than that of acid phosphatase. And at all the stages there are no significant differences between both control and treatment groups of alkaline phosphatase. The activities of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase decreased gradually from the final instar larva until the ten day pupa stage and increased at the newly emerged adult stage. The activity of succinic acid dehydrogenase decreased gradually until the six day pupa stage and increased at the eight day pupal stage. In the final instar larva stage and the two day adult stage the activities reached a maximum. Endogenous respiration was very high at the two day pupal stage, and decreased at the six day pupa, followed by an increased at the late pupal stages. Succinate in the respiratory substrate increased respiratory activity in all stages and had a striking influence on both the late pupal stage and the adult. Hence, changes in total protein concentration, protein patterns, trehalose, lipids, mineral salts, nucleic acid, acid · alkaline phosphatase activity, activities of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase and succinic acid dehydrogenase through the course of the physiological cycle form a U-shaped curve when graphed against the histolysis of larval organs and the histogenesis of adult organs. In view of controlling pest, the U-shaped pattern of physiological activity indicates that control will be the most effective at the time of the most physiological weakness manifested at the four day pupal stage.

신령버섯(아가리쿠스) 분말이 고지방을 섭취한 흰쥐의 단백질과 무기질 농도 및 효소활성에 미치는 영향

고진복 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-

고지방식이에 신령버섯 분말 첨가 식이를 섭취한 숫쥐의 성장률, 장기무게, 단백질과 무기질 농도 및 효소활성에 미치는 영향을 조사하고자, 성숙한 숫쥐에 표준식이를 급여한 정상군, 표준식이에 20% 돈지를 첨가한 식이를 급여한 고지방군, 고지방 식이에 신령버섯 자실체 분말을 3% 및 5%씩 첨가한 식이를 급여한 군(3% 및 5% 신령버섯군) 등 4군으로 나누어 10주간 사육한 결과는 다음과 같다. 실험동물의 체중증가량 및 식이효율은 고지방군에 비해 3% 신령버섯군이 다소 감소되었으나, 5% 신령버섯군은 유의하게 감소되었다. 간, 신장, 비장 및 고환의 무게는 고지방군과 신령버섯군들이 비슷하였으나, 부고환지방은 고지방군에 비해 신령버섯군들이 유의하게 감소되었다. 혈청의 단백질, 혈색소, 혈당, 칼슘, 무기인 및 마그네슘 농도는 정상군과 각 실험군이 비슷한 수준으로 신령버섯 섭취에 따른 영향은 나타나지 않았다. 혈청의 GOT, GPT, r-GTP, LDH 및 alkaline phosphatase의 활성은 정상군과 각 실험군이 유의한 차이는 나타나지 않았다. 이상의 결과로 보아 고지방식이에 신령버섯분말을 5% 첨가한 식이가 성숙한 흰쥐의 체중증가와 부고환지방 축적을 억제하는 효과가 나타났다. 혈청의 단백질, 혈색소, 혈당, 칼슘농도 및 효소활성은 정상수준을 유지하였다. The effects of the fruiting body of Agaricus blazei Murill on the weight gains, food intakes, food efficiency ratios, serum and hepatic lipids concentrations were investigated in male rats. Sprague-Dawley adult rats, were given four different types of diets for a succeeding period of 10 weeks: either a normal diet (5% corn oil), a high fat diet (high fat; 20% lard), a 3% or 5% Agaricus diet (high fat diet + 3% or 5% Agaricus powder). The body weight gains and food efficiency ratios of the rats fed 5% Agaricus diet were significantly lower than those of the rats fed the high fat diet. The epididymal fat pad weights of the rats fed 3% or 5% Agaricus diets were significantly lower than those of the rats fed the high fat diet. The weights of hepatic, kidney, spleen and epididymis in the rats fed Agaricus diets were similar to those in the rats fed the high fat diet. There were no differences in the serum concentrations of protein, albumin, and calcium, phosphorus, magnesium, and enzyme activity among the experimental groups. These results showed that the 5% Agaricus diet feeding decreased the body weight and epididymal fat pad weight.

남생이(Geoclemys reevesii) 소뇌에 있어서 raf Protein Kinase의 면역세포화학적 분포

고진복 新羅大學校 1993 論文集 Vol.36 No.1

Raf oncogene is a transforming gene of retrovirus. 3611-murine sarcoma virus(3611-MSV) and there are active genes known in man. a-raf, b0raf and c-raf, A-raf and c-raf encode cytoplasmic protein related with serine/threonine specific protein kinase activity and also they are similar to protein kinase C in the functional structure and cellular distribution. In this study. the distribution of a-raf and coraf protein kinase was researched in the cerebellum of Geoclemys reevesii. Generally raf protein kinase was distributed limitedly in Purkinje cell layer. granular cell layer. and molecular cell layer of cerebellum. Especially it was strongly immunoreactive in the Purkinje cell. In Geoclemys reevesii, it was notable that the immunolabeling of a-raf protein kinase was shown a higher intensity than that of c-raf protein kinase.

단백질 수준이 암ㆍ숫쥐의 단백질대사에 미치는 영향

고진복 신라대학교 자연과학연구소 1995 自然科學論文集 Vol.1 No.-

The female(30-week-old) and male(60-week-0ld) Sprague-Dawley rats were fed experimental diets containing 10%, 18% and 24% protein for 4 weeks. Total protein and albumin concentrations in serum as well as hemoglobin and hematocrit value in blood were not affected by dietary protein levels. The concentrations of Urea nitrogen in serum of female and male rats were significantly higher in 18% and 24% protein diets compared with those in 10% protein diet. The levels of urinary urea nitrogen excretion of male rats were increased according to the increase in dietary protein levels. But the ratio of urea nitrogen to total urinary nitrogen was not influenced by dietary protein levels. The ratio of urea nitrogen/creatinine in serum and urinary excretion of female and male rats increased with increasing levels of protein intake. The ratio of urea nitrogen/creatinine in serum and urinary excretion of female and male rats increased with increasing levels of protein intake. The ratio of urea nitrogen/creatinine in urine of male rats for 10% protein diet group was 7.3, that was showed poor nutritive condition of protein. The concentrations of uric acid in liver of female and male rats fed high protein (24% protein) diet were significantly higher than those of the 10% and 18% protein diets. The apparent absorption and balance of nitrogen were significantly higher in high protein than those in low protein than those in low protein diet. Nitrogen absorption rates were as follows : 85.4%(female), 85.5%(male) for 10% protein diet, 89.4%, 91.3% for 18% protein diet and 92.8%, 93.5% for 24% protein diet in female and male rats respectively All experimental groups mere positive in nitrogen balance.

蛋白質水準이 血淸 및 肝 脂質濃度에 미치는 영향

高鎭福 新羅大學校 1988 論文集 Vol.25 No.-

This expriment was conducted to study the effect of different levels of protein on serum and liver lipids in rats. The concentrations of serum and liver total lipid, total cholesterol and serum high density lipoprotein(HDL) cholesterol as well as hemoglobin and hematocrit value were measured after feeding mature male (60 week-old) and female (30 week-old) Sprague-Dawley rats for 4 weeks. The three dietary protein levels used were 10, 18 and 24%. Serum and liver total lipid concentrations of male rats in 10% protein group were higher than those in 18% and 24% protein group, but the female rats had no difference in each diet group. Hemoglobin and hematocrcit value as well as serum and liver total cholesterol concentrations of the male and female rats were not affected by dietary protein levels. Serum HDL-cholesterol concentrations and HDL-cholesterol per total cholesterol ratio ofmale rats significantly increased in 18% protein group compared with 10% and 24% protein groups, but the female rats had no difference in the levels of protein.

정신지체 초등학생의 국어과 듣기 말하기 영역 성취기준의 수행수준

고진복 한국특수아동학회 2014 특수아동교육연구 Vol.16 No.2

This study aimed to examine the actual condition of performance level of Korean language art listening and speaking area in 2011 revised special education curriculum of students at the mental retardation special elementary school. Following this study purpose, the study selected 'how are the performance level by grades and performance level by achievement standards of Korean language art listening and speaking area are?' as a study problem. To solve this study problem, author let teachers with the experience of instructing 126 students from 2 mental retardation special elementary schools evaluate their performance level by use of questionnaire. The summary of study results was as follows. First, the performance level of listening and speaking of students at the mental retardation special elementary school became low as their grade increased. Looking into the performance level of first-second grade students, while the students who had trouble in performing were under 10%∼20%, the rate of third-fourth grade and fifth-sixth grade students having trouble in performance was higher than one of first-second grade students and the difference of performance level in the grade appeared big. Second, comparing the performance level of listening and speaking area, the more they rose to upper grade, the lower the performance level of speaking appeared than listening. As well, it was shown that the hierarchy and performance level of achievement standard by each grades suggested in curriculum did not conform. Third, looking into the performance level by achievement standards of listening and speaking area, the difference of performance level among achievement standards suggested in one core element appeared bigger than the difference of performance level among achievement standards suggested in the core element of the same grade. As well, there were many differences from the achievement standard performance level among each grade. 이 연구는 정신지체 특수학교 초등학생의 2011 개정 특수교육 교육과정 국어과 듣기 및 말하기 영역의 수행수준의 실태를 알아보는데 목적이 있다. 이러한 연구목적에 따라 국어과 듣기 및 말하기 영역의 학년군별 수행수준과 성취기준별 수행수준이 어떠한가를 연구문제로 선정하였다. 이와 같은 연구문제를 해결하기 위해 정신지체 특수학교 2곳의 초등학생 126명을 대상으로 설문지를 이용하여 연구대상자를 직접 지도한 경험이 있는 교사들에게 학생들의 수행수준을 평가하도록 하였다. 연구결과를 요약하면 다음과 같다. 첫째, 정신지체 특수학교 초등학생의 듣기와 말하기의 수행수준은 학년군이 올라감에 따라 수행수준이 낮아지는 것으로 나타났다. 1-2 학년군의 수행수준을 살펴보면, 수행이 곤란한 학생이 10%∼20% 이하인 반면, 3-4 학년군과 5-6 학년군은 1-2 학년군 보다 수행이 곤란한 학생의 비율이 높고 학년군 내의 수행수준 차이가 크게 나타났다. 둘째, 듣기와 말하기 영역의 수행수준을 비교하여 보면, 학년군이 올라감에 따라 말하기가 듣기보다 낮은 수행수준을 나타냈다. 또한, 교육과정에 제시된 각 학년군별 성취기준의 위계와 수행수준이 일치하지 않은 것으로 나타났다. 셋째, 듣기와 말하기 영역의 성취기준별 수행수준을 살펴보면, 같은 학년군의 중핵요소에 제시된 성취기준 간 수행수준 차이보다 하나의 중핵요소에 제시된 성취기준 간 수행수준의 차이가 더 크게 나타났다. 또한, 각 학년군 간 성취기준 수행수준과도 차이가 많이 나타났다.

누에분말이 당뇨 암쥐의 혈당, 지질 및 단백질 농도에 미치는 영향

고진복,오세원 신라대학교 자연과학연구소 2000 自然科學論文集 Vol.8 No.-

Effects of silkworm powder(Bombyx mori L) feeding on blood levels of glucose, lipids and protein in diabetic and nondiabetic rats were studied. Female rats(Sprague-Dawley, mean weight 313.6± 18.5 g) were assigned to three dietary groups and fed experimental diets for 5 weeks. For each experimental, some rats were injected with streptozotocin intraperitoneally(i.p.) to induce diabetes, and other rats were injected with buffer i.p. as a control group. The body weights of diabetic control group(CD group) and silkworm powder group(SP group) were significantly decreased(-97.8∼-99.1 g) compared with the control group(3.5 g). The liver and pancrease weights were similar to all groups. The spleen weight CD and SP groups were significantly decreased compared with the control group. The level of fasting serum glucose were higher in CD and SP groups than that of the control group. The total cholesterol, HDL-cholesterol concentrations and HDL-cholesterol/total cholesterol ratio of serum were similar to all groups. The triglyceride, LDL-cholesterol concentrations of serum were significantly decreased in SP groups than in those CD group. The total lipid and triglyceride concentrations of liver in CD and SP groups significantly decreased compared with the control group. The total cholesterol concentration of liver was lower in SP group than in that CD group. The total protein and albumin concentrations of serum in CD and SP groups were decreased compared with control group.