과산화수소증기 시스템을 이용한 야전용 천막 내 Geobacillus stearothermophilus 아포 제독

윤성녀,김윤기,정정훈,유현상,민경률,김민철,김세계,류삼곤,Yoon, Sung Nyo,Kim, Yun Ki,Jeung, Jeung Hoon,Yoo, Hyun Sang,Min, Kyung Yool,Kim, Min Cheol,Kim, Se Kye,Ryu, Sam Gon 한국군사과학기술학회 2016 한국군사과학기술학회지 Vol.19 No.5

The purpose of this study is to demonstrate the suitability of hydrogen peroxide($H_2O_2$) vapor system for platform interior decontamination. Geobacillus stearothermophilus biological indicator(BI) strips and a field tent were used as a biological simulant and as a simulated platform, respectively. Decontamination was performed based on injection rates and tent sizes with exposure time 60 minutes. We standardized the conditions for the field tent decontamination : 8.0 g/min for $30m^3$($H_2O_2$ vapor concentration of 150~500 ppm, relative humidity of 50 %) and 12.0 g/min for $60m^3$($H_2O_2$ vapor concentration of 250~400 ppm, relative humidity of 55 %). Thus we suggest the system is one of the possible candidates for decontamination of platform interiors.

Analysis of Correlation between Atmospheric Fluorescent Particles and Biomaterials

윤성녀,이재경,김덕호,유현상,민경율,김민철 한국대기환경학회 2018 Asian Journal of Atmospheric Environment (AJAE) Vol.12 No.4

Bio-aerosols are important components of atmospheric aerosols, which can affect human health depending on the origin of biological particles. A real-time monitoring technology for bio-aerosols can rapidly provide information on the current state of biological particles in ambient environment, which substantially minimize the impact of hazardous biological particles. The aim of this study is to evaluate LIF (laser-induced fluorescence) technology on real-time monitoring of bio-aerosols by investigating the correlation between biomaterial concentration (cultured microbes or extracted DNA) and fluorescent particle concentration in ambient environment. For this, we used LIF instrument, BDS (Bio-aerosol Detection System), and analyzed the correlation coefficients from the collected data (the non-fluorescent particle concentration, the fluorescent particle concentration, the cultured microbial concentration, the extracted DNA concentration). Pearson’s correlation coefficients (r) from this study are as follows: 0.85 (p<0.001) between the fluorescent particle concentration and the cultured microbial concentration, 0.93 (p< 0.001) between the fluorescent particle concentration and the extracted DNA concentration, while 0.42 (p<0.01) between the non-fluorescent particle concentration and the cultured microbial concentration, 0.49 (p<0.01) between the non-fluorescent particle concentration and the extracted DNA concentration. It was also found that the size range of the fluorescent particles, which exhibited the highest coefficient for the extracted DNA concentration and the cultured microbial concentration, was 2 to less than 10 micrometer (μm), and their coefficients were 0.89 (p<0.01) and 0.8 (p<0.001), respectively. These results indicate that the fluorescent particles, especially in the range of 2 to less than 10 μm, are highly correlated with bio-aerosols rather than the non-fluorescent particles. Taken together, LIF technology is great for real-time monitoring of bio-aerosols by counting fluorescent particles and resolving particle sizes at ambient atmospheric environment.

원형질체 융합에 의한 인산염 제거균주의 개량

윤성녀,조경주,조홍범,최영길 한국미생물학회 1991 미생물학회지 Vol.29 No.2

In order to improve the removal ability of phosphate, Spheroplast fusions were performed among auxotrophic mutants of Aeromonas hydrophila isolated from waste water, named A13 and A14, Aci37 auxotrophic mutant of Acinetobactercalcoaceticus, and auxotrophic E. coli HR262/pCE27 carring pit gene. Eight fusants obtained from this experiment showed different biochemical characteristics. When the rate of phosphate uptake among fusants (F1-F8) was investigated in Phosphate Uptake Medium (PUM), F8 strain showed the highest rate for phosphate removal, 7 times as much as control after two hours incubation. The role of cations ($Mg^{++}$ ,$Ca^{++}$ , $K^{+}$ in phosphate uptade by F8 was also investigated in PUM without each salt. $K^{+}$ seemed to be crucial. Being compared with phosphate untake rate in PUM, that in PUM without $K^{+}$ was reduced 1.5 times. Therefore, by applying F8 strain and $K^{+}$ in practical environmental system, the increased efficiency in phosphate removal can be derived.

B-1. Induction of Ribonuclease during Catabolic Repression in Saccharomyces uvarum

최영길,윤성녀 한국균학회 1985 韓國菌學會誌 Vol.13 No.4

과산화수소 증기 시스템을 이용한 미생물 제독에 관한 연구

김윤기,김민철,윤성녀,황현철,류삼곤,Kim, Yun Ki,Kim, Min Cheol,Yoon, Sung Nyo,Hwang, Hyun Chul,Ryu, Sam Gon 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.3

Objectives: Effectiveness and conditions of vapor-phase hydrogen peroxide (VPHP) system on decontamination of Geobacillus stearothermophilus(GS) spores, Escherichia coli (E.coli) and Enterobacteria phage felix01 (felix01) were determined. Methods: The VPHP system was designed to vaporize 35% (w/w) solution of hydrogen peroxide, continuously to inject and withdraw VPHP. The system and VHP 1000ED (Steris) were operated such that dehumidification and conditioning were initiated without samples in the chamber. Then the samples were loaded into and removed. Coupons (glass, anodizing, silicon, viton) with GS spores ($1{\times}10^6$ colony forming unit/mL [CFU/mL]), E.coli ($1{\times}10^7$ CFU/mL) and felix01 ($1{\times}10^7$ plaque forming unit/mL[PFU/mL]), and Biological Indicator (BI) with GS spores ($1{\times}10^6$ CFU/mL) on stainless steel coupons were used. The tested samples were sonicated and vortexed, and then were plated for enumeration, followed by incubation at $55^{\circ}C$, 24 hr for GS spores, and at $37^{\circ}C$, 24 hr for E.coli and felix01. BI analysis in broth culture was only qualitative. Results: The efficacy of the VPHP system on decontamination was almost equivalent to that of VHP 1000ED. The conditions for complete decontamination with the VPHP system was as follows: concentration; 700~450 ppm, relative humidity; approximately 55%, and temperature; $34{\sim}32^{\circ}C$. When comparing the decontamination efficiency among different kinds of coupons, glass was the most effective, however, all kinds of coupons were decontaminated completely after 60 min exposure in both systems. Conclusion: The VPHP system can be recommended as an alternative system for traditional system using ethylene oxide, formaldehyde or chlorine dioxide.

Saccharomyces uvarum 의 Catabolic Repression 시기에 유도되는 Ribosomal Ribonuclease 에 대한 연구

이기성,최영길,윤성녀 한국균학회 1986 韓國菌學會誌 Vol.14 No.3

In order to study subcellular locality and characteristics of ribonuclease in Saccharomyces uvarum, subcelllar fractions 45,000 x g pellet fraction, post ribosomal fraction and ribosome fraction were extracted during late log, stationary phase and sugar starvation conditions. Ribonuclease activity was significantly increased in ribosomal fraction under stationary and sugar starvation conditions. Ribosomal ribonuclease was extracted by EDTA plus streptomycin sulfate sad ammonium sulfate precipitation. The amount of ribosome in stationary and sugar starvation condition was decreased three to six fold as compared to that in the early log phase. The end products of ribosomal ribonuclease were detected by thin layer chromatography. It is postulated that the increase of ribosomal ribonuclease activity under sugar starvation results from 5'-rRNase, while the increase of rRNase activity under stationary phase results from 3'-rRNase.

과산화수소 제독 과정에서의 탄저균 전사체 분석

김상훈,김세계,정경화,윤성녀,김윤기,김민철,류삼곤,이해완,채영규,Kim, Sang Hoon,Kim, Se Kye,Jung, Kyoung Hwa,Yoon, Sung Nyo,Kim, Yun Ki,Kim, Min Cheol,Ryu, Sam Gon,Lee, Hae Wan,Chai, Young Gyu 한국군사과학기술학회 2015 한국군사과학기술학회지 Vol.18 No.4

Decontamination of biological agents utilizes hydrogen peroxide($H_2O_2$) for its effectiveness and safeness. Bacillus anthracis is a major target for $H_2O_2$ decontamination. To assess the effect of $H_2O_2$ on B. anthracis and identify biomarkers for decontamination, whole transcriptomic profiling of $H_2O_2$-treated B. anthracis was performed. Here we identified deregulation in stress response genes, transcription factors and cellular homeostasis genes. We also found that expression of antisense RNAs increased in B. anthracis during decontamination. We postulate that B. anthracis prioritizes survival and adaptation in response to $H_2O_2$ treatment by changing its gene expression pattern.

Klebsiella aerogenes의 카드뮴 적응 및 해독기작에 관련된 생리생화학적인 변화

이기성,송인극,박영식,윤성녀,최영길 한국미생물학회 1990 미생물학회지 Vol.28 No.3

In the course of operating the accommodative and detoxifying mechanism against cadmium, its physio-biochemical changes were observed in Klebsiella aerogenes ATCC 10031. Changes of enzyme activity concerned phosphate metabolism, changes of phospholipid composition and in view of energy metabolism the changes of the nucleotide pool were examined. Activities of both alkaline and acid phosphatase were derepressed 4-10 folds under cadmium added cultures. Moreover, production of phospholipid such as lysophosphatidyl choline (LPC), phosphatidyl serine (PS) and phosphatidyl ethanolamone (PE) was increased and uridylate nucleotide pool was increased under Cd-surplus culture. These results i.e. overproduction of phosphatase catalyzing phosphate residue, increase of the production of PE and PS which have a close affinity with cadmium, and indrease of uridylate nucleotide pool used as a carrier of polysaccharide synthesis like bacterial capsule exhibited cellular responses for active defence against Cd-pressure. It was postulated that these phenomena should play another assistant roles in Cd-detoxifing mechanism.