Controlled ovalbumin release from nanoporous multilayer structure

양미소,홍진기 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0

Multilayer nano films based on various materials has been studied in a wide range of biomedical application fields such as drug delivery or cell culture environments. In this study, we have developed nanoporous multilayer structure to control protein release manners. Gold nanoparticles are assembled with biocompatible polymers and dissolved by post treatment. By control parameters during layer-by-layer (LbL) assembly, multilayer thickness, porosity, and distribution of nanoparticles were easily tuned. Take full advantages of nanoporous structure, antigen protein: ovalbumin was successfully loaded. Control a drug release mechanism was corresponded with multilayer structure degradation rate. To confilm nanoporous structure of nanofilm, SEM, ellipsometer and profilometer were used. In summary, we established a LbL technique-based nanoporous nanofilm system that allows the continuous release of ovalbumin.

Nanoporous multilayer films for controlled drug release

양미소,홍진기 한국고분자학회 2014 한국고분자학회 학술대회 연구논문 초록집 Vol.2014 No.4

Nanoporous multilayer films for controlled antigen protein release

양미소,홍진기,최다희,최문현 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.33 No.-

We have studied the preparation and characterization of nanoporous thin films fabricated from a layerby layer assembly of branched poly(ethylene imine), gold nanoparticles (gold NPs) and hyaluronic acid. Gold NPs embedded in the multilayer thin film structure were easily dissolved using an aqueous cyanidesolution, generating the nanoporous film. This allows for the direct and precise comparison of theporosity of the polymer based multilayer thin films in both the presence and the absence of gold NPs. Additionally, the layer by layer (LbL) assembly method has advantages in that it increases thepossibilities for mass production of multilayer films, and allows the precise control of film structuresprepared on large substrates. Therefore, this method offers a new route to allow the introduction ofnanoporosity into multilayer thin films, allowing the development of more complex functionalmulticomponent nanoporous structures for protein delivery. Furthermore, these novel materials couldbe used in biomedical applications.

Layer-by-Layer Assembled RGD-Peptide Nano-Films on BMSCs

최다희,양미소,허지웅,홍진기 한국고분자학회 2015 한국고분자학회 학술대회 연구논문 초록집 Vol.2015 No.10

Procyanidin C1 Causes Vasorelaxation Through Activation of the Endothelial NO/cGMP Pathway in Thoracic Aortic Rings

변의백,성낙윤,양미소,송두섭,변의홍,김재경,박종흠,송범석,이주원,박상현,변명우,김재훈 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.7

The aim of this study was to clarify the efficacy of procyanidin C1 (Pro C1) for modulating vascular tone. Pro C1 induced a potent vasorelaxant effect on phenylephrine-constricted endothelium-intact thoracic aortic rings, but had no effect on denuded thoracic aortic rings. Moreover, Pro C1 caused a significant increase in nitric oxide (NO) production in endothelial cells. Pro C1-induced vasorelaxation and Pro C1-induced NO production were significantly decreased in the presence of a nonspecific potassium channel blocker (tetraethylammonium chloride [TEA]), an endothelial NO synthase inhibitor (NG-monomethyl-L-arginine [L-NMMA]), and a store-operated calcium entry inhibitor (2-aminoethyl diphenylborinate [2-APB]). Pro C1-induced vasorelaxation was also completely abolished by an inhibitor of soluble guanyl cyclase, which suggests that the Pro C1 effects observed involved cyclic guanosine monophosphate (cGMP) production. Interestingly, Pro C1 significantly enhanced basal cGMP levels. Taken together, these results indicate that Pro C1-induced vasorelaxation is associated with the activation of the calcium-dependent NO/cGMP pathway, involving potassium channel activation. Thus, Pro C1 may represent a novel and potentially therapeutically relevant compound for the treatment of cardiovascular diseases.

Characterization of Proinflammatory Responses and Innate Signaling Activation in Macrophages Infected with Mycobacterium scrofulaceum

김기혜,김태성,Joy G. Lee,박정규,양미소,김진만,조은경,육재민 대한면역학회 2014 Immune Network Vol.14 No.6

Mycobacterium scrofulaceum is an environmental andslow-growing atypical mycobacterium. Emerging evidencesuggests that M. scrofulaceum infection is associated withcervical lymphadenitis in children and pulmonary or systemicinfections in immunocompromised adults. However, the natureof host innate immune responses to M. scrofulaceum remainsunclear. In this study, we examined the innate immuneresponses in murine bone marrow-derived macrophages(BMDMs) infected with different M. scrofulaceum strains includingATCC type strains and two clinically isolated strains(rough and smooth types). All three strains resulted in theproduction of proinflammatory cytokines in BMDMs mediatedthrough toll-like receptor-2 and the adaptor MyD88. Activation of MAPKs (extracellular signal-regulated kinase1/2, and p38, and c-Jun N-terminal kinase) and nuclear receptor(NF)-κB together with intracellular reactive oxygenspecies generation were required for the expression of proinflammatorycytokines in BMDMs. In addition, the rough morphotypesof M. scrofulaceum clinical strains induced higherlevels of proinflammatory cytokines, MAPK and NF-κB activation,and ROS production than other strains. When micewere infected with different M. scrofulaceum strains, thoseinfected with the rough strain showed the greatest hepatosplenomegaly,granulomatous lesions, and immune cell infiltrationin the lungs. Notably, the bacterial load was higherin mice infected with rough colonies than in mice infectedwith ATCC or smooth strains. Collectively, these data indicatethat rough M. scrofulaceum induces higher inflammatoryresponses and virulence than ATCC or smoothstrains

Gamma-Irradiated Luteolin Inhibits 3-Isobutyl-1-Methylxanthine-Induced Melanogenesis Through the Regulation of CREB/MITF, PI3K/Akt, and ERK Pathways in B16BL6 Melanoma Cells

변의백,송하연,Sajid Mushtaq,김혜민,강정애,양미소,성낙연,장범수,변의홍 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.8

Luteolin was gamma irradiated at doses of 0, 15, 30, 50, 70, and 100 kGy. We observed that the luteolin peak decreased simultaneously with the appearance of new radiolytic peaks, using high-performance liquid chromatography (HPLC). The highest new radiolytic peak gamma-irradiated luteolin (GLM) was observed at a dose of 70 kGy, and the GLM was identified by nuclear magnetic resonance and high-performance-liquid-chromatography-quadrupole-time-of-flight (HPLC-Q-TOF) mass spectrometry. We examined whether 70 kGy gamma-irradiated luteolin has more effective anti-melanogenic effects than intact luteolin. Seventy kilograys of gamma-irradiated luteolin inhibited melanin synthesis and intracellular tyrosinase activity without cytotoxicity, whereas the intact luteolin-treated group did not show anti-melanogenic activity in 3-isobutyl-1-methylxanthine-stimulated B16BL6 melanoma cells. The expression of melanogenic enzymes, such as tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2, was decreased by 70 kGy gamma-irradiated luteolin treatment, owing to the suppression of microphthalamia-associated transcription factor and 3′,5′-cyclic adenosine monophosphate (cAMP) response element binding protein. In addition, gamma-irradiated luteolin decreased the phosphorylation of phosphoinositide 3-kinase (PI3K)/Akt and extracellular regulated kinase (ERK). The anti-melanogenic effects of 70 kGy gamma-irradiated luteolin were attenuated by the treatment of two specific inhibitors (PD98059 and LY294002), and these results indicate that the anti-melanogenic effects were mediated by ERK and PI3K signaling pathways. Therefore, our findings suggest that gamma-irradiated luteolin can be a potential cosmeceutical agent for skin whitening.

감마선 조사가 커피박 추출물의 색도 및 생리활성에 미치는 영향

송하연(Ha-Yeon Song),김혜민(Hye-Min Kim),김우식(Woo Sik Kim),양미소(Mi-So Yang),변의홍(Eui-Hong Byun),장범수(Beom-Su Jang),최대성(Dae Seong Choi),변의백(Eui-Baek Byun) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.5

본 연구는 폐자원인 커피박의 산업적 이용도를 증진시키기 위하여 감마선 조사를 이용하여 커피박 추출물의 색도 개선에 관하여 평가하기 위해 수행되었다. 커피박 추출물로부터 유용성 성분을 얻기 위하여 70% 에탄올로 추출한 후 감마선 조사를 한 결과 30, 50 kGy의 조사선량에서 커피박 추출물의 색도가 개선된 것을 확인하였다. 30, 50 kGy로 감마선 조사된 커피박 추출물에서 성분변화가 일어났지만, 항산화능력과 미백활성능에는 0 kGy 커피박 추출물 처리군과 비교했을 때 유의적인 변화가 없는 것을 확인하였다. 이는 다른 성분들이 감마선 조사에 의해 감소하였지만, 퀸산이 상당량 증가함으로써 성분변화로 인한 커피박 추출물의 생리활성능 감소를 보완한 것으로 보이며, 이상의 결과로 감마선 조사가 커피박 추출물의 진한 갈색도를 개선함으로써 커피박 추출물이 화장품 소재로 활융될 수 있는 부가가치를 부여할 수 있으며, 이러한 폐기물의 재활용을 통해 커피박 폐기로 인한 환경문제 및 경제적 문제점을 개선하는데 기여할 수 있을 것으로 판단된다. The spent coffee grounds (SCG) are considered valuable by-products because they contain various bioactive compounds. The SCG extraction (SCGE) was irradiated at doses ranging between 30 and 50 kGy. The deep dark-brown color of SCGE was changed to a bright yellow color by gamma irradiation. The content of the bioactive compounds of gamma-irradiated SCGE was analyzed by high-performance liquid chromatography. Interestingly, the content of quinic acid was increased by gamma irradiation, whereas other compounds were decreased. Although the contents of bioactive compounds were changed by gamma irradiation, the biological activities (radical scavenging activity and whitening effects) of SCGE were unaffected. Our findings suggest that gamma irradiation can effectively improve the color values of SCGE without the loss of biological activities. Consequently, gamma irradiation can be a useful tool for improving the utilization of SCGE in the cosmetic industry.

산소발생반응을 위한 CuCo₂O₄ 나노섬유 전기화학 촉매 합성 및 특성 분석

원미소(Mi So Won),장명제(Myeong-Je Jang),이규환(Kyu Hwan Lee),김양도(Yang Do Kim),최승목(Sung Mook Choi) 한국표면공학회 2016 한국표면공학회지 Vol.49 No.6

The non-noble 1D nanofibers(NFs) prepared by electrospinning and calcination method were used as oxygen evolution reaction (OER) electrocatalyst for water electrolysis. The electrospinning process and rate of solution composition was optimized to prepare uniform and non-beaded PVP polymer electrospun NFs. The diameter and morphology of PVP NFs changed in accordance with the viscosity and ion conductivity. The clean metal precursor contained electrospun fibers were synthesized via the optimized electrospinning process and solution composition. The calcined CuCo₂O₄ NFs catalyst showed higher activity and long-term cycle stability for OER compared with other Co₃O₄, NiCo₂O NF catalysts. Furthermore, the CuCo₂O₄ NFs maintained the OER activity during long-term cycle test compared with commercial CuCo₂O₄ nanoparticle catalyst due to unique physicochemical and electrochemical properties by1D nanostructure.