Low Molecular Weight β-Glucan Stimulates Doxorubicin-induced Suppression of Immune Functions in Mice

성낙윤,변의백,송두섭,유영춘,김재경,박종흠,송범석,박상현,이주운,유영법,김재훈 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.3

The aim of this study was to evaluate the protective effect of low molecular weight β-glucan (LMG)against doxorubicin (DOX)-induced immune suppression of tumor-bearing mice. The tumor size and spleen cell functions such as spleen cell proliferation, cytokine production (interferon-γ and interleukin-2), and the population of CD4+ and CD8+ T cells were estimated. In the tumorbearing mice, the tumor size was significantly (p<0.05)decreased by DOX treatment. However, there was no significant difference between mice treated with high molecular weight β-glucan (HMG) and mice treated with LMG. Spleen cell proliferation and cytokine production were significantly (p<0.05) decreased in only DOX treated group, but increased in all β-glucan treated groups with DOX. Moreover, the populations of CD4+ and CD8+ T cells were also increased in the LMG-treated group. It appears that LMG effectively reduces the DOX-induced immune toxicity through activation of immune cells such as splenocytes.

감마선 조사가 열처리 겨우살이의 항당뇨 및 세포독성에미치는 영향

박종흠,김수민,성낙윤,송두섭,변의백,김재경,송범석,이주운,김재훈 한국방사선산업학회 2013 방사선산업학회지 Vol.7 No.2

Mistletoe (Viscum album) has been widely used as a functional food material for varioustherapeutic purposes from ancient time. In this study, we examined anti-diabetic and cytotoxicactivities of heated-treated mistletoe and the effects of gamma-irradiation on its activities. Heattreatedmistletoe extract was prepared by heating during different time (3, 6, 9 and 12 h) andgamma-irradiated with different doses of 0, 10, 30, 50, 70 and 100 kGy. Heat-treated mistletoeextracts showed a concentration-dependent cytotoxicity on rat insulinoma RINm5F cells and theeffect was gradually decreased as heating time increased up to 12 h. 12 h heat-treated extract wasno cytotoxic. Gamma-irradiation enhanced the reduction of heat-treated mistletoe-inducedcytotoxicity and the decreasing effect was an irradiating dose-dependent. In particular, all of 70kGy irradiated and heat-treated mistletoe extracts did not showed the cytotoxicity and the effectwas comparable to 12 h heat-treated mistletoe extract. Among those extracts, 3 h heat-treatedmistletoe extract gradually increased the insulin secreting activity by gamma-irradiation and theeffect was the best at 70 kGy, whereas 12 heat-treated extract was no effect. On the test of α-glucosidaseinhibitory activity, 3 h heat-treated mistletoe extract showed the concentration dependenteffects and gamma-irradiation induced more activity at 70 kGy, compared to non-irradiated 3 hand 12 h heated mistletoe extracts. These results suggest that the combination of heat treatmentand gamma-irradiation might be more effective than only heat-treatment for improving the antidiabeticactivity of mistletoe extract and reducing its cytotoxicity.

구연산/칼슘/인산염 용액을 이용한 토양 중금속 안정화

송호철(Ho Cheol Song),송두섭(Doo Sup Song),조동완(Dong Wan Cho),박성원(Sung Won Park),최상훈(Sang Hun Choi),전병훈(Byong Hun Jeon),이장호(Jang Ho Lee),박준홍(Joon Hong Park) 大韓環境工學會 2009 대한환경공학회지 Vol.31 No.4

거풍광산 주변 밭의 오염된 중금속을 토양오염공정시험법과 TCLP를 이용하여 분석한 결과 비소, 납, 카드뮴, 구리, 아연과 같은 중금속의 농도가 전국 토양 평균치를 초과하는 것으로 나타났다. 본 연구에서는 중금속 오염된 토양의 안정화를 위해서 구연산칼슘인산염 용액을 이용하는 방안에 대한 기초연구를 실험실 조건과 현장 조건에서 수행하였다. 실험실 조건에서 구연산칼슘인산염 용액을 투여한 실험 결과, 고압멸균처리한 반응조에 비해 토착미생물 군집이 존재하는 반응조에서 용존 인산염의 농도가 현격히 감소하였으며, 중금속 안정화율도 높다는 것을 확인하였다. 현장 실험에서도 구연산칼슘인산염 용액 투여가 용존인산염 감소와 중금속 안정화 결과를 가져왔다. 이는 미생물의 인산염 사용이 중금속 안정화 향상에 도움이 되었음을 의미한다. 현장 실험에서 미생물군집을 분석한 결과, 구연산칼슘인산염 용액 투여로 다양성이 증대되었으며, Anaerofilum과 Treponema같은 혐기성 미생물 개체가 우점종으로 발현되는 결과를 얻었다. 이러한 결과들을 살펴볼 때, 구연산칼슘인산염의 투여로 인한 토착미생물 신진대사 활성화를 통해서 토양 내 혐기성 환원 조건을 제공하거나 토양미생물이 인산염을 중금속 안정화에 이용되기 쉽게 도와주는 미지의 기작을 통해서 해당 현장의 중금속 안정화에 기여한 것으로 해석된다. A farming area located near an abandoned copper mine in GuPo-ri, Choongchung province is heavily contaminated with heavy metals such as As, Pb, Cd, Cu and Zn of which concentrations are higher than the values typically detected in Korean soil environment. In this work, laboratory and field studies were conducted to examine feasibility of using Ca-citrate-phosphate solution in stabilizing heavy metals in the polluted soils. In laboratory batch experiments with field soil, the addition of Ca-citrate-phosphate solution resulted in decrease of aqueous phase concentration of phosphate and improvement of heavy metal stabilization, compared to those for sterilized soil samples. This indicates that microbial uptake of phosphate may have provided positive effects on availability of phosphate toward heavy metal stabilization. According to microbial community analysis for the field experiment, the use of Ca-citrate-phosphate led to increased diversity of microbial populations, and strict anaerobic microorganisms such as Anaerofilum and Treponema became the most dominant populations in the solution-amended field experiments. These findings suggest that, when Ca-citrate-phosphate is used for heavy metal stabilization in soils, microbial processes may have important roles in improving the stabilization of heavy metals by providing reducing conditions to the treatment locations or/and by making phosphate available to heavy metal stabilization.

감마선 조사가 Staphylococcal Enterotoxin B의 비장세포 증식률 및Interleukin-2 분비능에 미치는 영향

박종흠,성낙윤,변의백,송두섭,김재경,송범석,김재훈,이주운,유영춘 한국방사선산업학회 2013 방사선산업학회지 Vol.7 No.2

The purpose of this study was to investigate the cell proliferating and interleukin-2producing activity of staphylococcal enterotoxin B by gamma-irradiation. Staphylococcal enterotoxinB was gamma-irradiated with the various doses of 0, 2, 20 and 50 kGy. SDS-PAGE analysisshowed that gamma-irradiation caused the sharp decrease of the content of staphylococcal enterotoxinB and the effect was irradiating dose-dependent. Non-irradiated staphylococcal enterotoxinB increased the cell proliferation of splenocytes isolated from female Balb/c mouse, whereas 2kGy-irradiated toxin significantly decreased the activity. 20 and 50 kGy-irradiated staphylococcalenterotoxin B was no effect. A similar effect on the interleukin-2 production of mouse splenocyteswas observed with non-irradiated and irradiated staphylococcal enterotoxin B. It was consideredto be due to the decrease of the antigenicity of staphylococcal enterotoxin B by gamma-irradiation. Therefore, these results suggest that gamma-irradiation can be effective for the decrease of theantigenicity of staphylococcal enterotoxin B as superantigen.

면역조혈계에 대한 실크단백질 산 가수분해물의 방사선 방어 효과

김재경,이지혜,성낙윤,변의백,송두섭,박종흠,최종일,송범석,이주운,이정용,연성호,김재훈 대한방사선방어학회 2012 대한방사선방어학회 학술발표회 논문요약집 Vol.2012 No.04

전자선 조사가 베타글루칸의 항알레르기 활성에 미치는 영향

박종흠,성낙윤,변의백,송두섭,김재경,송범석,박상현,신미혜,이주운,김재훈,유영춘 한국방사선산업학회 2012 방사선산업학회지 Vol.6 No.3

This study evaluated the change in anti-allergy activity of β-glucan by electron beamirradiation. β-Glucan was irradiated at dose of 50 kGy and then orally pre-treated with electronbeam irradiated and non irradiated β-Glucan for 7 days. After pre-treatment, allergy was inducedby injection of ovalbumin (OVA). Serum total immunoglobulin E (IgE) and OVA-specific IgE levelsin the allergic mice was significantly increased but the mice pre-treated 50 kGy electron beam irradiatedβ-glucan was significantly decreased the levels of total IgE and OVA-specific IgE, respectively. Moreover, cytokine production (interleukin-4) was also decreased in the 50 kGy electronbeam irradiated β-Glucan pre-treated mice. These results indicate that pre-treatment of 50 kGyelectron beam irradiated β-glucan may elevate the anti-allergy activity. Therefore, electron beamirradiatedβ-glucan could be used for nutraceutical foods in food industry.

Procyanidin C1 Causes Vasorelaxation Through Activation of the Endothelial NO/cGMP Pathway in Thoracic Aortic Rings

변의백,성낙윤,양미소,송두섭,변의홍,김재경,박종흠,송범석,이주원,박상현,변명우,김재훈 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.7

The aim of this study was to clarify the efficacy of procyanidin C1 (Pro C1) for modulating vascular tone. Pro C1 induced a potent vasorelaxant effect on phenylephrine-constricted endothelium-intact thoracic aortic rings, but had no effect on denuded thoracic aortic rings. Moreover, Pro C1 caused a significant increase in nitric oxide (NO) production in endothelial cells. Pro C1-induced vasorelaxation and Pro C1-induced NO production were significantly decreased in the presence of a nonspecific potassium channel blocker (tetraethylammonium chloride [TEA]), an endothelial NO synthase inhibitor (NG-monomethyl-L-arginine [L-NMMA]), and a store-operated calcium entry inhibitor (2-aminoethyl diphenylborinate [2-APB]). Pro C1-induced vasorelaxation was also completely abolished by an inhibitor of soluble guanyl cyclase, which suggests that the Pro C1 effects observed involved cyclic guanosine monophosphate (cGMP) production. Interestingly, Pro C1 significantly enhanced basal cGMP levels. Taken together, these results indicate that Pro C1-induced vasorelaxation is associated with the activation of the calcium-dependent NO/cGMP pathway, involving potassium channel activation. Thus, Pro C1 may represent a novel and potentially therapeutically relevant compound for the treatment of cardiovascular diseases.

DNA Microarry를 이용한 Deinococcus radiodurans의 구리이온 특이 반응 유전자 탐색 및 특성 분석

조민호,임상용,정선욱,송두섭,최영지,김동호,Joe, Min-Ho,Lim, Sang-Yong,Jung, Sun-Wook,Song, Du-Sub,Choi, Young-Ji,Kim, Dong-Ho 한국미생물학회 2008 미생물학회지 Vol.44 No.3

대표적인 방사선저항성 미생물인 Deinococcus radiodurans의 구리이온($CuCl_2,250{\mu}M$)에 대한 발현체 분석을 DNA microarray를 이용하여 수행하였다. 총 3,187개의 open reading frame중 70개의 유전자 발현이 2배 이상증가(64개) 또는 감소(6개)하였다. 이들 중 흥미롭게도 철이온 흡수 관련 유전자들로 추정되는 두 개 operon ($DRB0014{\sim}DRB0017$과 $DRB0121{\sim}DRB0125$)의 발현이 가장 높게 증가하였다. 두 operon의 첫 번째 유전자인 DRB0014와DRB0125의 발현을 실시간 정략 PCR을 이용하여 분석한 결과, 두 유전자 모두 구리 이온($CuCl_2,250{\mu}M$) 또는 철이온 킬레이트화합물(2,2'-dipyridyl, $ 250{\mu}M$)을 첨가하였을 경우 발현이 10배 이상 증가하였으나 철이온($FeCl_3,250{\mu}M$) 또는 구리 이온 킬레이트화합물(bathocuproine disulphonate, $250{\mu}M$)이 존재할 때에는 발현이 변화하지 않았다. 따라서 D. radiodurans의 구리 대사는 철 흡수 시스템과 관련이 있는 것으로 추정된다. 그러나 DRB0014와DRB0125의 변이는D. radiodurans의 구리 저항성 및 방사선 저항성에 큰 영향을 미치지 않는 것으로 관찰되었다. Global gene expression of Deinococcus radiodurans, a highly radiation resistant bacterium, in response to excess copper was analyzed by using oligonucleotide microarray chip. Among 3,187 open reading frames of D. radiodurans, seventy genes showed a statistically significant expression ratio of at least 2-fold changes under growth conditions of excess copper; 64 genes were induced and 6 genes were reduced. Especially, two operons ($DRB0014{\sim}DRB0017$ and $DRB0125{\sim}DRB0121$) presumably involved in the iron transport and utilization were the most highly induced genes by excess copper. A quantitative real-time PCR assay revealed that DRB00l4 and DRB0125 are highly transcribed responding to excess copper and 2,2'-dipyridyl, an iron chelator. In addition, the transcription of both genes was not changed by excess iron and bathocuproine disulphonate, a copper chelator. These results suggested that the copper metabolism may be closely connected with the iron transport and utilization in D. radiodurans. However, the disruption of each gene, DRB00l4 and DRB0125, did not affect the copper and radiation resistance, the most well-known character of this organism.

실크 단백질 유래 펩타이드의 in-vitro 방사선 방호 효과

박종흠,이지혜,성낙윤,변의백,송두섭,김재경,김재훈 대한방사선방어학회 2012 대한방사선방어학회 학술발표회 논문요약집 Vol.2012 No.04

감마선 조사에 의한 LPS의 면역 독성 저감화

박종흠,성낙윤,변의백,송두섭,송범석,김재경,이주운,박상현,김재훈,강근옥,유영춘 한국방사선산업학회 2012 방사선산업학회지 Vol.6 No.3

The purpose of this study is to reduce the immune toxicity of lipopolysaccharide (LPS)by gamma irradiation. LPS was gamma-irradiated at the various doses of 20, 100 and 200 kGyand then evaluated on the immune toxicity through the cell proliferation, nitricoxide productionand cytokine release. Cell proliferation significantly decreased in the intact LPS treated groups,whereas gamma-irradiated LPS treated group were not reduced the cell proliferation. Similarly,the production of nitric oxide and cytokine showed the high levels in the intact LPS treated group. However, gamma-irradiated LPS treated group remarkably decreased the production of nitricoxide and cytokine in dose-dependent manner. Therefore, gamma irradiation may effective methodto reduce the immune toxicity of LPS.