인삼 조사포닌의 새로운 분리 방법

신지영,최언호,위재준,Shin, Ji-Young,Choi, Eon-Ho,Wee, Jae-Joon 한국식품과학회 2001 한국식품과학회지 Vol.33 No.2

인삼의 약리활성 성분인 사포닌 성분의 추출분획 중 ginsenoside 함량을 높이거나 화학적 성분 변화를 줄이거나 또는 추출과정을 간편화하기 위한 새로운 추출 분획 방법을 정립하고 기존의 방법과 화학적 조성을 비교하였다. 인삼사포닌의 분획분리 방법으로서 일반적인 고온 MeOH/n-BuOH추출법(BuOH법) 및 고온 MeOH/Diaion HP-20법(HP-20법) 이외에도 새로운 3가지 방법, 즉 고온 MeOH 추출/cation AG 50W 흡착/$H_2O$ 용출/n-BuOH 추출법(AG 50W법), 상온 MeOH/Diaion HP-20법(상온추출법), EtOAc/n-BuOH 직접추출법으로 조사포닌을 분리하였다. AG 50W법에 의한 조사포닌 중 총 ginsenoside 함량은 61.5%로 가장 높았으며 반대로 단백질과 유리 아미노산 함량은 각각 0.93과 0.19%로 다른 방법에 비해 현저히 낮게 나타났다. 단백질의 함량은 HP-20법에 의한 분리가 14.18%로 가장 높았고, 유리당은 BuOH법이 13.5%로서 HP-20법 및 AG 50W법에 비해 $20{\sim}40$배 높은 것으로 나타나 유리당이 BuOH법에 의한 조사포닌 중 순수사포닌 함유 비율을 낮추는 한 요인임을 알 수 있다. 한편, 본 연구에서 새로이 정립한 AG 50W법의 경우 prosapogenin의 생성이 많았다. 그 밖에, 사포닌 분석시료의 신속한 조제를 위하여 추출과정을 단축하여 EtOAc/n-BuOH 혼합용매로 직접 환류추출할 경우, ginsenoside $Rg_1$과 Re의 조성이 높게 나타났다. 본 연구 결과는 조사포닌의 조제 방법에 따라 ginsenoside, 유리당 및 조단백질 등 화학성분의 조성이 현저히 다르며 실험목적에 따라 적절한 방법이 이용될 수 있음을 시사한다. In order to increase ginsenoside content, to reduce chemical change, to shorten extracting procedure, new methods of extraction and fractionation of crude ginseng saponin were established and compared for their chemical composition. Those are hot MeOH extraction/n-BuOH fractionation (BuOH method) and hot MeOH extraction/Diaion HP-20 adsorption/MeOH elution (HP-20 method), which are already known methods, and additional three new methods: hot MeOH extraction/cation AG 50W $adsorption/H_2O$ elution/n-BuOH extraction (AG 50W method), cool MeOH extraction/Diaion HP-20 adsorption/MeOH elution (cool extraction method) and direct extraction with EtOAc/n-BuOH (direct extraction method). AG 50W method provided a crude saponin showing the highest content of ginsenosides of 61.5% and the lowest contents of protein and free amino acids of 0.93% and 0.19%, respectively. The protein content was the highest as 14.18% in the crude saponin by HP-20 method, while free sugar content was the highest as 13.5% by BuOH method, indicating that these are factors that lower the rate of ginsenoside in crude saponins by those methods. On the other hand, it was revealed that AG 50W method produced large amount of prosapogenins during the pass through the cation exchange resin (AG 50W) column being strongly acidic. Crude saponin from direct extraction method showed relatively higher composition of ginsenoside $Rg_1$ and Re. The results suggest that contents and composition of ginsenosides and other chemical components in crude ginseng saponin greatly depend on the condition of the extraction and fractionation.

순수 및 혼합냉매의 유동증발 열전달 상관식

신지영,김민수,노승탁,Shin, J.Y.,Kim, M.S.,Ro, S.T. 대한설비공학회 1996 설비공학 논문집 Vol.8 No.2

Boiling heat transfer coefficients of pure refrigerants(R22, R32, R125, R134a, R290, and R600a) and refrigerant mixtures(R32/R134a and R290/R600a) are measured experimentally and compared with several correlations. Convective boiling term of Chen's correlation predicts experimental data for pure refrigerants fairly well(root-mean-square error of 12.1% for the quality range over 0.2). An analysis of convective boiling heat transfer of refrigerant mixtures is performed for an annular flow to study degradation of heat transfer. Annular flow is the subject of this analysis because a great portion of the evaporator in refrigeration or air conditioning system is known to be in the annular flow regime. Mass transfer effect due to composition difference between liquid and vapor phases, which is considered as a driving force for mass transfer at interface, is included in this analysis. Correction factor $C_F$ is introduced to the correlation for the pure substances through annular flow analysis to apply the correlation to the mixtures. The flow boiling heat transfer coefficients are calculated using the correlation considering nucleate boilling effect in the low quality region and mass transfer effect for nonzazeotropic refrigerant mixtures.

돌연변이 T7 RNA 증합효소의 머리핀 구조가 만들어지지 않는 인자독립형 전사종결 부위에서의 전사종결에 관한 연구

신지영,김동희,이상수,Shin, Ji-Young,Kim, Dong-Hui,Lee, Sang-Soo 배재대학교 자연과학연구소 2004 自然科學論文集 Vol.14 No.1

T7 RNA 중합효소는 두 종류의 인자 독립형 전사 종결신호를 인지하여 전사연장을 종결하게 되는데 이 중 머리핀 구조가 만들어지지 않는 PHT와 CJ 전사종결 부위는 ‘ATCTGTT' non-template 염기서열 다음에 T염기가 많은 부분이 존재하는 형태로 이루어져 잇다. 이와 같은 전사종결을 민감하게 인지하는 돌연변이 T7 RNA 중합효소인 X4, X19, BG8과 이와 반대로 둔감하게 인지하는 R173C를 제조하여 전사활성도를 측정하였다. 이결과 야생형 T7 RNA 중합효소에 비해 X4는 8%, X19는 33%, BG8은 34%의 전사활성도를 보여 전사활성도가 상당히 감소하는 결과를 보였다. 반면에 R173C는 야생형에 비해 112%의 전사활성도를 보여 야생형과 거의 유사한 전사활성도를 보였다. 또한 PTH 및 CJ 전사종결 부위를 전사주형으로 이용하여 전사하였을 때 X4, X19, BG8은 야생형 RNA중합효소에 비해 종결 부위에서의 종결이 증가하였으나 R173C는 거의 PTH 및 CJ 전사종결부위에서 전사종결이 일어나지 않고 전사가 진행되는 것을 확인하였다. T7 RNA polymerase also recognize intrinsic, hairpin-independent termination signal, a conserved 7-base pair sequence (ATCTGTT in the non-template stand) and U-rich sequence downstream of it. These intrinsic, hairpin-independent termination signal were commonly found in PTH and CJ termination sequences. There are two types of mutant T7 RNA polymerases recognizing sensitively(X3, X19, BG8) of insensitively (R173C) the intrinsic termination signals. We determined the T7 transcription activities of these mutants. Compared to wild-type, mutants X4, 19 and BG8 show highly reduced transcription activities (8%, 33%, 34%). On the other hand mutant R173C shows comparable transcription activity of wild-type (112%). Also transcription termination efficiencies at the PTH or CJ termination signals were determined by using mutant RNA polymerases. Temination of mutnats X4, X19 and BG8 are increased compared to wild-type. On the other hadn mutant R173C proceeds through PTH and CJ termination signals.

The Complete Mitochondrial Genome of Nysius plebeius Distant, 1883 (Heteroptera: Lygaeidae) from Korea

신지영,라메스워 마하르잔,이휘종,정민규,김주일,Jiyeong Shin,Rameswor Maharjan,Hwijong Yi,Minkyu Jeong,Juil Kim Korean Society of Applied Entomology 2023 한국응용곤충학회지 Vol.62 No.2

Nysius plebeius is a major lygaeid pest of various cereal crops and ornamental plants in East Asian countries, including Korea. The complete mitochondrial genome of N. plebeius was characterized and found to comprise a total of 17,367 bp, which included 13 protein-coding genes, NADH dehydrogenase components (complex I, ND), cytochrome oxidase subunits (complex VI, COX), cytochrome oxidase b (CYPB), two ATP synthases, two ribosomal RNA genes, and 22 transfer RNAs. The GC content of 23%. It showed high sequence similarity to other Lygaeidae species, such as N. cymoides (94.5%), N. fuscovittatus (91.7%), and an unknown Nysius species (94.1%). This new N. plebeius mitochondrial genome can be widely used for evolutionary studies of Lygaeidae and to improve pest management practices.

약강구조를 포함하는 영어단어에 대한 영어학습자의 약음절 지각과 반응시간(I)

신지영,김기호,김희성,Shin Ji-Young,Kim Kee-Ho,Kim Hee-Sung 대한음성학회 2006 말소리 Vol.57 No.-

The purpose of this study is to observe how Korean learners perceive an English weak syllable in words containing WS syllable pattern. According to the automated discrimination task using E-Prime, the ratio of correct answer(%) and reaction time of the stimuli with same syllable patterns were respectively higher and faster than those with different syllable patterns. Specifically, in the stimuli with different syllable patterns, the frequency(familiarity) of stressed word succeeding weak syllable and whether the weak syllable had coda in it were two important factors in distinguishing between a word with and without weak syllable. Even though the high English proficiency Koreans had faster reaction time than the low English proficiency Koreans, all Korean learners had a difficulty in perceiving the weak syllable at the beginning of a word.

염기서열 특이적 전사종결부위에서 T7 RNA 중합효소 전사연장복합체 특성에 관한 연구

신지영,이상수,Shin, Ji-Young,Lee, Sang-Soo 배재대학교 자연과학연구소 2004 自然科學論文集 Vol.14 No.1

박테리오 파아지 T7 RNA 중합효소는 다른 RNA 중합효소와 비교하여 볼 때 보조인자 없이 전사를 진행하는 하나의 subunit로 구성된 RNA 중합효소이다. 전사 진행 단계 중에서 T7 RNA 중합효소의 전사연장을 연구하기 위해 biotin이 결합된 DNA 주형을 streptavidin bead로 고정시킴으로서 T7 RNA 중합효소의 진행과정을 관찰할 수 있었고, 이러한 기작을 이용하여 일련의 활성을 가지는 가장 안정한 전사연장복합체들을 얻을 수 있었다. 전사 연장체들은 16번 염기 위치로부터 18번 염기의 위치까지 방사선 동위원소가 표지되어 있으며 이들 표지된 전사연장복합체들은 단계별로 합성하여 22-40개 핵산잔기들이 합성된 전사연장복합체들을 얻을 수 있었다. 이와 같은 전사연장복합체들을 PTH 전사종결 부위가 있는 주형으로 사용하여 야생형 및 R173C 돌연변이 RNA 중합효소를 이용하여 전사연장복합체를 제조하여 비교한 결과 PTH 전사종결에 둔감한 R173C 돌연변이 중합효소의 경우 야생형에 비해 PTH 전사종결부위를 지난 위치에서도 전사연장복합체가 생성되었다. T7 RNA polymerase is a single subunit RNA polymerase able to accomplish whole transcription process without auxiliary factors. In order to study transcription elongation mechanism of phage T7 RNA polymerse, stepwise walking of RNA polymerase was established by immobilizing biotinylated DNA template with streptavidin bead, series of active and stable elongation complexes were obtained, Transcripts were radio isotope labeled at the 16thm 17th and 18th nucleotide residues so stable elongation transcription complex of T7 RNA polymerase containing 22-40 nucleotide residues could be identified. We identified the positions of stablely formed transcription elongation complexes of termination site in intrinsic hairpin-independent PTH terminator sequence through the established stepwise walking of wild-type of mutant R173C T7 RNA polymerases. The results suggest that stable elongation transcription complexes were at the site of passing PTH terminator signal by mutant R173C RNA polymerase.

두 가지 장비를 이용한 각막곡률 및 각막고위수차의 비교

신지영,이민영,정소향,Ji Young Shin,Min Young Lee,So Hyang Chung 대한안과학회 2014 대한안과학회지 Vol.55 No.12

Purpose: To investigate the correlations of keratometry and corneal high order aberrations (HOAs) between the Keratron Scout videokeratoscope and the Pentacam HR Scheimpflug camera. Methods: From December 2012 to February 2013, keratometry and corneal HOAs were determined using the Keratron Scout videokeratoscope and Pentacam HR Scheimpflug camera in 23 healthy individuals (46 eyes). Results: Average keratometry showed high correlation with 95% confidence interval -0.155 ± 0.37 between the Keratron Scout and Pentacam HR. When comparing HOAs of the Keratron Scout and total corneal HOAs of Pentacam HR, total root-meansquare (RMS), and spherical aberration were positively correlated between the 2 devices (r = 0.67, <EM>p </EM>< 0.001 and r = 0.74, <EM>p</EM> < 0.001, respectively). When comparing HOAs of Keratron Scout and anterior corneal HOAs of Pentacam HR, total RMS, spherical aberration and coma were positively correlated between the 2 devices (r = 0.62, <EM>p </EM>< 0.001, r = 0.81, <EM>p </EM>< 0.001, and r = 0.52, <EM>p</EM> = 0.047, respectively). Conclusions: Although the 2 devices are based upon different principles, the Keratron Scout videokeratoscope and Pentacam HR Scheimpflug camera showed positive correlations in keratometry, total RMS, spherical aberration, and coma. Both devices may be useful for clinical applications. J Korean Ophthalmol Soc 2014;55(12):1758-1764

수직축 풍력터빈 블레이드의 공기역학적 특성

신지영,손영석,차득근,이철균,황이철,Shin, Jee-Young,Son, Young-Seok,Cha, Duk-Guen,Lee, Cheol-Gyun,Hwang, I-Cheol 한국마린엔지니어링학회 2006 한국마린엔지니어링학회지 Vol.30 No.8

The objective of this study is to investigate the aerodynamic characteristics of a vertical axis wind turbine blade as the basic study of a design of a vertical axis wind turbine. The lift and drag coefficients of the various shape of the vortical axis wind turbine blades are analyzed and compared using the CFD code Fluent. To validate the numerical analysis, the predicted results of the Fluent are compared with those of the Xfoil code and the experimental results. We conclude that the program Fluent can be used to predict the aerodynamics of the wind turbine blade. By comparing the predicted results of the aerodynamic characteristics of the different shape of the blades, an appropriate shape of the blade is suggested to design the vortical axis wind turbine blade.

들뢰즈와 바디우의 사건 개념에 비추어 본 종교 현상과 그 윤리

신지영(SHIN, Ji-Young) 프랑스학회 2010 프랑스학연구 Vol.53 No.-

En face des phenomenes pathologiques qui s' apparaissent sous Ie nom de religion, nous proposons d'exarniner la pensee de Gilles Deleuze et Alain Badiou a propos de la religion, d' une part pour en faire un diagnostic et d' autre part pour redefinir la religion dans Ie contexte de la philosophie modeme. Les verites repensees chez eux sont definies comme evenement et vie, et leur pensee a propos de la religion en tant que processus des verites pourrait se trouver autour du concept d' evenement. Alors nous pla<;ons une vie comme fondement de la religion, anti-dialectique comme son processus, et presentons Ie sysreme de signes comme objet de la pensee religieuse, et I' apOtre comme son agent ethique. Du point de vue de cette philosophie d' evenement, des phenomenes religieux deveraient pathologiques et montreraient des affinites avec Ie pouvoir social, des attitudes justificatives pour des ordres existants, des perspectives negatives envers les faibles sociaux, des positions exclusives contre quelques groupes sociaux etc., si elle essayait de fixer des verites a I' aide de I'identite et la partialite, dans Ie processus de la dialectique. Ainsi, avec Ie concept d'evenement chez Deleuze et Badiou, nous pouvons repenser la religion dans Ie contexte totalement nouveau, dit, modeme, et emettre un diagnostic des phenomenes religieux.

노인 폐결핵의 특징

신지영 ( Ji Young Shin ),정선영 ( Sun Young Jung ),이정은 ( Jeong Eun Lee ),박지원 ( Ji Won Park ),유수진 ( Su Jin Yoo ),박희선 ( Hee Sun Park ),김주옥 ( Ju Ock Kim ),김선영 ( Sun Young Kim ) 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.69 No.3

Background: Pulmonary tuberculosis remains a health concern in Korea despite major progress in the development of new strategies for diagnosing and treating tuberculosis. In particular, the diagnosis of newly developed pulmonary tuberculosis is on the rise in elderly persons. The aim of this study was to investigate the clinical, radiographic characteristics, and treatment outcomes of pulm onary tuberculosis in the elderly. Methods: The medical records of 113 young (<65 years old) and 112 elderly (≥65 years old) pulmonary tuberculosis patients diagnosed at Chungnam National University hospital between January 2007 and December 2008 were reviewed. Results: There was no difference in the prevalence of typical symptoms between the younger and the elderly group. Dypsnea was the only symptom that occurred more frequently in the elderly group (16.8% vs 5.5%, p=0.008). On radiological study, pneumonic infiltration type was more common in the elderly group (28.6% vs 16.8%, p=0.035). Sputum Acid fast bacilli smear positivity rate was similar between the 2 groups. Elderly patients with anti-tuberculosis medication had more frequent adverse drug reactions; however, there was no significant difference between the 2 groups in the number of patients required to stop medication due to an adverse drug reaction. There were more patients lost to follow-up in the elderly group (22/112, 19.6% vs 11/113, 9.7%, p=0.036). Conclusion: The majority of elderly patients did not complete the treatment, resulting in a poorer outcome. Therefore, we need to make an effort to support the continued screening of elderly patients by making this economically feasible.