정자의 형태학적 특성 분석에 관한 연구

백재승,전성수,김수웅,이원진,박광석,Paick, Jae-Seung,Jeon, Seong-Soo,Kim, Soo-Woong,Yi, Won-Jin,Park, Kwang-Suk 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.2

In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%${\sim}$-3.8%. The Pearson correlation coefficient between readers was 0.79. Between the manual and sperm morphology analyzer, the same findings were reported. In this experiments the slides were stained by two different methods, PAP and Diff-Quik staining methods. The limits of agreement were 7.2%${\sim}$-5.7% and 6.0%${\sim}$-6.3%, respectively. The Pearson correlation coefficients ware 0.76 and 0.91, respectively. The limits of agreement was tighter below 20% normal forms. In the experiments of repeatability, 52 cells stained by PAP and Diff-Quik staining methods were analyzed three times in succession. Estimating pairwise agreement, the kappa statistic for the pairs were 0.76, 0.81, 0.86, and 0.75, 0.88, 0.88 respectively. In this study it was shown that there was good agreement between manual and computerized assessment of normal and abnormal cells. The repeatability and agreement per slide of computerized sperm morphology analyzer was excellent. The computer's ability to classify normal morphology per slide is promising. Based on results obtained, this system can be of clinical value both in andrology laboratories and IVF units.

사정관 폐쇄

백재승,이안기,이희영,Paick, Jae-Seung,Lee, Ahn-Kie,Lee, Hee-Yong 대한생식의학회 1988 Clinical and Experimental Reproductive Medicine Vol.15 No.2

We found reports of only about 20 cases of well documented ejaculatory duct obstruction presenting with infertility and treated by TUR. While infertility caused by ejaculatory duct obstruction is rare accurate diagnosis by vasoseminal vesiculography is important, since these obstructions may be treatable. In our 29 patients undergoing vasoseminal vesiculography for 1 year to demonstrate obstruction site in seminal tract the ejaculatory duct obstruction was found in 6 patients. The intial methods of treament were TUR of the ejaculatory duct in 2 patients, forceful lavage of ejaculatory duct through the vas deferens in 4 patients. One patient's wife who had undergone TUR of the ejaculatory duct delivered a normal female baby 15 months postoperatively. In one patient who had undergone forceful lavage of ejaculatory duct semen analysis returns to normal level except the motility. When initial methods of treatment fail to get the normal semen we have the plan to perform TUR of the ejaculatory duct or aspiration of seminal fluid in the seminal vesicle under the control of the ultrasonography for AIH according to the level of ejaculatory duct obstruction. Our report suggests that ejaculatory obstruction has been underdiagnosed and should receive more attention by urologist.

Effects of Nitrofurantoin on Lipid Peroxidation and Reactive Oxygen Radical Generation in Porcine Lung Microsome

백재승,김시황,김혜원,정명희,김명석,Paick, Jae-Seung,Kim, Si-Whang,Kim, Hae-Won,Chung, Myung-Hee,Kim, Myung-Suk The Korean Society of Pharmacology 1985 대한약리학잡지 Vol.21 No.1

항균제 nitrofurantion에 의한 폐독작용의 생화학적 기전을 규명하기 위한 연구 일환으로 in vitro에서 폐장 microsome 지질의 과산화 및 반응성 산소 radical $(O^{-}_{2}{\cdot},\;H_2O_2,\;OH{\cdot},\;^1O_2)$의 생성에 대한 nitrofurantion의 영향과 양자 간의 상호 관련성을 검토하였다. Nitrofurantion은 호기성 반응 조건에서 돼지 폐장 microsome의 NADPH 의존성 지질 과산화를 용량 의존적으로 증가시킬 뿐 아니라 $O^{-}_{2}{\cdot},\;H_2O_2$ 및 두 radical의 상호 작용으로 2차적으로 형성되는 $OH{\cdot}$의 생성 또한 촉진하였으며 $^1O_2$생성은 관찰되지 않았다. 이와 같은 폐장 microsome지질 과산화 증가는 SOD 및 catalase에 의하여 억제될 뿐만 아니라 $OH{\cdot}$ 제거 물질인 mannitol, thiourea에 의하여도 현저히 억제되었으며, $^1O_2$ 제거 물질에 의하여는 영향을 받지 않았던 한편 염기성 반응 조건에서는 nitrofurantoin에 의한 지질 과산화가 관찰되지 않았다. 이상의 결과로 미루어 보아 nitrofurantoin은 폐장 microsome의 NADPH 의존적이 반응성산소 radical $(O^{-}_{2}{\cdot},\;H_2O_2$ 및 $OH{\cdot})$의 생성을 증가시키며 이들 중 특히 $OH{\cdot}$ 에 의한 microsome막 지질 과산화를 촉진하는 것으로 결론지었고, 이와 같은 in vivo 현상은 nitrofurantion의 in vitro 폐독작용의 기전을 설명하는 일부가 될 것으로 사료하였다. In vitro effects of nitrofurantoin, an antimicrobial agent for acute and chronic urinary tract infection, on the lung microsomal lipid peroxidation and the generation of reactive oxygen radicals were investigated to elucidate the biochemical mechanisms of its in vivopulmonary toxicity. The interaction of nitrofurantoin with porcine lung microsome resulted in significant lipid peroxidation. In addition, nitrofurantoin stimulated the generation of reactive oxygen radicals, $O^{-}_{2}{\cdot},\;H_2O_2$ as well as a highly reactive secondary oxygen species, $OH{\cdot}$. The stimulation of lipid peroxidation was inhibited not only by superoxide dismutase and catalase, but also by hydroxyl radical scavengers, mannitol and thiourea. Neither singlet oxygen $({^1}O_{2})$ was detected during the incubation of microsome with nitrofurantoin, nor lipid peroxidation was inhibited by singlet oxygen scavengers. When incubated anaerobically under the nitrogen atmosphere, the ability of nitrofurantoin to stimulatle lipid peroxidation was abolished. It appears that NADPH-dependent metaboliam of nitrofurantoin in pulmonary microsome under aerobic condition is accompanied by the stimulation of lipid peroxidation through the mediation of reactive oxygen radicals, particularly hydroxyl radical. It is strongly suggested from these results that the stimulation of pulmonary microsomal lipid peroxidation by the reactive oxygen radical may be a in vivo mechanism of pulmonary toxicity caused by nitrofurantoin.

Nitrofurantion이 폐장 미크로솜 지질과산화와 반응성 산소 라디칼 생성에 미치는 영향

백재승(Jae-Seung Paick),김시황(Si-Whang Kim),김혜원(Hae-Won Kim),정명희(Myung-Hee Chung),김명석(Myung-Suk Kim) 대한약리학회 1985 대한약리학잡지 Vol.21 No.1

항균제 nitrofurantion에 의한 폐독작용의 생화학적 기전을 규명하기 위한 연구 일환으로 in vitro에서 폐장 microsome 지질의 과산화 및 반응성 산소 radical (O₂^-⋅, H₂O₂, OH⋅, ¹O₂)의 생성에 대한 nitrofurantion의 영향과 양자 간의 상호 관련성을 검토하였다. Nitrofurantion은 호기성 반응 조건에서 돼지 폐장 microsome의 NADPH 의존성 지질 과산화를 용량 의존적으로 증가시킬 뿐 아니라 O₂^-⋅, H₂O₂ 및 두 radical의 상호 작용으로 2차적으로 형성되는 OH⋅의 생성 또한 촉진하였으며 ¹O₂생성은 관찰되지 않았다. 이와 같은 폐장 microsome지질 과산화 증가는 SOD 및 catalase에 의하여 억제될 뿐만 아니라 OH⋅ 제거 물질인 mannitol, thiourea에 의하여도 현저히 억제되었으며, ¹O₂ 제거 물질에 의하여는 영향을 받지 않았던 한편 염기성 반응 조건에서는 nitrofurantoin에 의한 지질 과산화가 관찰되지 않았다. 이상의 결과로 미루어 보아 nitrofurantoin은 폐장 microsome의 NADPH 의존적이 반응성산소 radical (O₂^-⋅, H₂O₂ 및 OH⋅)의 생성을 증가시키며 이들 중 특히 OH⋅ 에 의한 microsome막 지질 과산화를 촉진하는 것으로 결론지었고, 이와 같은 in vivo 현상은 nitrofurantion의 in vitro 폐독작용의 기전을 설명하는 일부가 될 것으로 사료하였다. In vitro effects of nitrofurantoin, an antimicrobial agent for acute and chronic urinary tract infection, on the lung microsomal lipid peroxidation and the generation of reactive oxygen radicals were investigated to elucidate the biochemical mechanisms of its in vivopulmonary toxicity. The interaction of nitrofurantoin with porcine lung microsome resulted in significant lipid peroxidation. In addition, nitrofurantoin stimulated the generation of reactive oxygen radicals, O₂^-⋅, H₂O₂ as well as a highly reactive secondary oxygen species, OH⋅. The stimulation of lipid peroxidation was inhibited not only by superoxide dismutase and catalase, but also by hydroxyl radical scavengers, mannitol and thiourea. Neither singlet oxygen (¹O₂) was detected during the incubation of microsome with nitrofurantoin, nor lipid peroxidation was inhibited by singlet oxygen scavengers. When incubated anaerobically under the nitrogen atmosphere, the ability of nitrofurantoin to stimulatle lipid peroxidation was abolished. It appears that NADPH-dependent metaboliam of nitrofurantoin in pulmonary microsome under aerobic condition is accompanied by the stimulation of lipid peroxidation through the mediation of reactive oxygen radicals, particularly hydroxyl radical. It is strongly suggested from these results that the stimulation of pulmonary microsomal lipid peroxidation by the reactive oxygen radical may be a in vivo mechanism of pulmonary toxicity caused by nitrofurantoin.

Hough변환을 이용한 정자의 형태학적 특성 분석방법에 관한 연구

박광석,이원진,백재승,Park, Kwang-Suk,Yi, Won-Jin,Paick, Jae-Seung 대한의용생체공학회 1996 의공학회지 Vol.17 No.1

Hough변환을 이용하여 정자의 행태학적 특성을 분석하는 새로운 방법을 개발하였다. 이 방법은 정자의 머리부분이 주위의 배경과 밝기의 차이를 갖는다는 특성과 함께 그 형태가 타원에 근접한다는 특성에 근거하고 있다. 정자의 머리부분은 타원을 나타내는 5개의 파라메터로 표현되고, 최적의 파라메터 값이 반복적인 Hough 변환에 의하여 검출된다. 일차적으로 정자가 있는 영역을 선택한다. Hough변환을 이용한 계산을 가능하게 하기 위하여 Hough변환된 공간의 크기를 최소한으로 줄이고, 검출된 오차가 최소화되는 방향으로 검사영역을 이동 시켜가며 최적의 파라메터들을 도출한다. 형태학적 특성을 나타내는 지표들은 정자의 형태를 나타내는 타원의 다섯개 파라메터들로부터 계산된다. A new analytic method has been developed for the analysis of sperm morphology using Hough transform. This method is based on the characteristic that sperm heads have elliptic shape in addition to the density difference with the background Sperm heads are represented in elliptic form with five parameter, and the optimal parameters are estimated by iterative Hough transform. To reduce processing time practically, we restricted the transformed space in minimum volume and moved the searching volume to the maximum gradient for the estimated error. Morphological parameters were calculated from estimated sperm head boundaries without further processing.

영상처리를 이용한 정자의 운동 특성 분석

심훈섭,이원진,박광석,백재승,Shim, Hoon-Sup,Yi, Won-Jin,Park, Kwang-Suk,Paick, Jae-Seung 대한전자공학회 1994 전자공학회논문지-B Vol.b31 No.11

본 논문에서는 영상처리 방법을 이용하여 정자의 운동 특성을 분석하는 자동화된 방법을 개발하였다. 시스템의 구성은 별도의 전용 프로세서를 사용하지 않고 PC와 간단한 영상처리 보드로 이루어진다. 영상 처리 보드는 영상을 받아들이는 데 사용되며 PC는 영상을 처리한 다음 분석해서 정자의 운동 특성을 나타내 주는 특성 변수의 값을 계산한다. 분석 알고리듬으로서 중요한 것은 정자의 위치 검출 알고리듬과 정자의 운동 경로를 추적하는 Match matrix 방법이다. 분석한 결과를 수작업 방법 그리고 전용 프로세서를 이용하는 방법과 비교하여 신뢰할 만한 결과를 얻었다. In this paper, we developed an analyzing method of the motional characteristics of sperm, using image processing technology. Without the aid of a dedicated image-processor, this processing of a personal computer(PC) and a simple image processing board. The image processing board is used for acquiring images from a microscopic imaging source. The PC processes the images from the board and computes the parameters of motional characteristics of sperms. The algorithm of the site detection of sperms and the 'Match Matrix Method' is noteworthy. After comparing the results of our method with those of the manual method, and with those of the method using a dedicated image-processor, we concluded that our method is useful and reliable.

신선 고환조직 정자와 냉동보존-융해 고환조직 정자를 이용한 난자세포질내 정자주입술 결과의 비교 연구

최영식,최영민,김수웅,백재승,지병철,구승엽,서창석,김석현,김정구,문신용,Choi, Young-Sik,Choi, Young-Min,Kim, Soo-Woong,Paick, Jae-Seung,Jee, Byung-Chul,Ku, Seung-Yup,Suh, Chang-Suk,Kim, Seok-Hyun,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2008 Clinical and Experimental Reproductive Medicine Vol.35 No.2

목 적: 무정자증 불임부부에서 신선 (fresh) 고환정자 (testicular spermatozoa)와 냉동보존-융해(cryopreserved-thawed) 고환정자를 사용한 난자세포질내 정자주입술 (intracytoplasmic sperm injection, ICSI)의 결과를 비교하고자 하였다. 연구방법: 신선 고환정자 및 냉동보존-융해 고환정자를 사용하여 ICSI 시술을 시행하기로 계획된 총 109주기 (66명)를 대상으로 하였고 신선 고환정자를 사용하기로 계획한 군 (신선 고환정자군, fresh group)에는 92주기 (61명)이 포함되었고 냉동보존-융해 고환정자를 사용하기로 계획한 군 (냉동보존-융해 고환정자군, cryopreserved-thawed group)에는 17주기 (13명)가 포함되었다. 양 군간에 수정률, 착상률, 임신률, 유산률 등 ICSI 시술의 결과들을 비교하였고 통계학적 분석은 Mann-Whitney U 검정 및 Fisher의 정확한 검정을 적절하게 사용하였다. 결 과: 신선 고환정자를 사용하여 ICSI 시술을 시행하기로 계획된 총 92주기 중 9주기에서 고환정자를 추출할 수 없어 시술 주기가 취소되었다. 냉동보존-융해 고환정자군과 비교하여 신선 고환정자군에서 수정률이 높은 경향을 보였고 ($58.0{\pm}27.8%$ vs. $45.9{\pm}25.0%$, p=0.076) 양질의 배아 수는 통계적으로 유의하게 높았다 ($0.9{\pm}1.2$ vs. $0.2{\pm}0.5$, p=0.002). 그러나 임상적 임신율, 착상률, 유산율은 양 군간에 통계적으로 유의한 차이가 없었다. 결 론: ICSI 시술을 위하여 냉동보존-융해 고환정자를 사용하는 경우 수정률 및 배아의 질이 감소하지만 임신율, 착상률, 유산율에는 영향을 미치지 않는 것으로 사료된다. 또한, ICSI 시술이전에 고환정자를 확보하고 냉동보존-융해 고환정자를 사용한다면 난자채취 당일 정자를 확보하지 못하여 주기를 취소하는 경우나 여성배우자의 불필요한 과배란유도를 줄일 수 있으며 반복적인 고환정자추출술로 인한 고환기능의 손상을 줄일 수 있는 유용한 방법으로 사료된다. Objective: To compare the outcomes of intracytoplasmic sperm injection (ICSI) with fresh and cryopreserved-thawed testicular spermatozoa in patients with azoospermia. Methods: One hundred and nine cycles (66 couples) where ICSI was planned with fresh or cryopreserved-thawed testicular spermatozoa were included in this study; Ninety two cycles (61 couples) with fresh testicular spermatozoa (fresh group) and seventeen cycles (13 couples) with cryopreserved-thawed testicular spermatozoa (cryopreserved-thawed group). We compared ICSI outcomes such as fertilization rate, implantation rate, pregnancy rate and miscarriage rate, which were statistically analyzed using Mann-Whitney U test or Fisher's exact test, where appropriate. Results: In 9 out of the 92 cycles where ICSI was planned with fresh testicular spermatozoa, testicular spermatozoa could not be retrieved. Fertilization rate tended to be higher in the fresh group than in the cryopreserved-thawed group ($58.0{\pm}27.8%$ vs. $45.9{\pm}25.0%$, p=0.076). The number of high quality embryos was significantly higher in the fresh group ($0.9{\pm}1.2$ vs. $0.2{\pm}0.5$, p=0.002). However, there were no significant differences in clinical pregnancy rate, implantation rate and miscarriage rate between the two groups. Conclusion: The results of this study suggest that although the use of cryopreserved-thawed testicular sperm for ICSI in patients with azoospermia may reduce fertilization capacity and embryo quality, it may not affect pregnancy rate, implantation rate and miscarriage rate. If testicular sperm can be obtained before ICSI procedure, the use of cryopreserved-thawed testicular sperm may also avoid unnecessary controlled ovarian hyperstimulation and cancellation of oocyte retrieval when spermatozoa cannot be retrieved as well as damage on testicular function by repeated TESE.

cis-Diamminedichloroplatinum(II) 내성 인체방광암 세포주에서 Bax 단백 발현

이경철(Kyung Chul Lee),변석수(Seok-Soo Byun),여운걸(Woon Geol Yeo),백재승(Jae-Seung Paick),이종욱(Chongwook Lee),이은식(Eunsik Lee) 대한비뇨기종양학회 2003 대한비뇨기종양학회지 Vol.1 No.1

N-butyl-N-(4-hydroxybutyl)nitrosamine 유도 백서 방광암 초기 발암 과정에서 Cyclooxygenase-2 (COX-2) 발현에 관한 연구

김정현(Jeong Hyun Kim),곽철(Cheol Kwak),박인애(In Ae Park),백재승(Jae-Seung Paick),이종욱(Chongwook Lee),이은식(Eunsik Lee) 대한비뇨기종양학회 2003 대한비뇨기종양학회지 Vol.1 No.3

낮은 복압성요누출압을 가진 요실금 환자에서 TVT 술식

백재승,김수웅,구자현,오승준,손환철,박관진,신재욱 대한배뇨장애 및 요실금학회 2003 International Neurourology Journal Vol.7 No.2