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Visualization of Bonghan Microcells by Electron and Atomic Force Microscopy
백구연,오가이,Sae Chae Jeoung,소광섭 사단법인약침학회 2009 Journal of Acupuncture & Meridian Studies Vol.2 No.2
Abstract Objectives: The origin of adult stem cells remains an open question. If they derive from embryos, it is difficult to determine the mechanism which interrupts their differentiation during tissue formation. In the 1960s, the Bonghan microcell was suggested as one possible, yet to be described, route of stem cell production, such that they have the potential to proliferate to produce normal cells. Materials and Methods: In this study, Bonghan microcells were isolated from Bonghan tissues on rat organ surfaces, and their detailed morphology examined by electron and atomic force microscopy. Results: The ultrastructure observed distinguished them from apoptotic bodies and other microorganisms, and their unique, possible proliferation feature, as protruding threads, was imaged by atomic force microscopy. Conclusions: The unique threadlike structure of the Bonghan microcell is consistent with Prof. Kim's observation in the first step of making a cell. Understanding of the functions of this threadlike structure may give a clue to understand the origin or the differentiation cue of adult stem cells.
Selective Toxicity on Canine Blood Cells by Using Atmospheric-pressure Plasma Jets
백구연,김용희,허은혜 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.60 No.6
Atmospheric pressure plasma jets have received increasing attention due to their non-thermal effects on biological samples. Their noticeable sterilization on tissues, selective apoptosis of cancer cells and effective regeneration of damaged tissues have successfully demonstrated their medical applicability. However, the side effects or toxic effects on normal cells should be investigated fully before real applications. In this study, we report the selective toxicity of atmospheric pressure plasma jets (APPJ) on blood cells in vitro for the first time. A complete blood cell count is used to count and size the blood cells statistically. We found gas- and time-dependent effects of APPJ exposure on the number ratios and the size distributions of red blood cells, white blood cells and platelets.
Fluorescent Method for Observing Intravascular Bonghan Duct
이병천,백구연,정현민,성백경,소경순,강대인,소광섭,Lee, Byung-Cheon,Baik, Ku-Youn,Johng, Hyeon-Min,Sung, Baeck-Kyoung,Soh, Kyung-Soon,Kang, Dae-In,Soh, Kwang-Sup KOREAN PHARMACOPUNCTURE INSTITUTE 2005 Journal of pharmacopuncture Vol.8 No.3
미분간섭현미경법과 아크리딘-오렌지(acridine-orange) 형광 염색을 이용하여 쥐 혈관 내의 실 모양 구조물을 관찰하였다. 공초점현미경법과 헤마톡실린-에오신(hematoxylin-eosin) 염색을 통해 피브린, 모세혈관, 소정맥, 소동맥 혹은 림프관의 핵 분포와 뚜렷이 구별되는 혈관 내 실 모양 구조물의 핵 분포 패턴을 얻어낼 수 있었다. 이 실 모양 구조물의 생리적 기능을 침술과 연관하여 논의하였다. 특히, 이 실 모양 관을 통한 약물의 흐름이 약침의 기전에 해당할 것이라는 가설을 제기하였다. Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.
Imaging of Fluorescent Nanoparticles Distributed in Mouse Skin
소광섭,백구연,V. Ogay,Y. W. Lee,J. K. Lee,S. H. Lee,Y. J. Kim 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2
Fluorescence imaging and electron microscopic image analysis are important tools to study the behavior and the distribution of nanoparticles in skin. We injected 50-nm-sized polyethylene-glycolcoated nanoparticles and studied their distribution and interaction at the injection site by using fluorescence microscopes and electron microscopes. We found two barriers, the basement membrane that kept the nanoparticles from diffusing to the epidermis layer and another barrier that prevented diffusion to the muscle layer. The uptake capability of various cells was also investigated. This biomedical physics study will be useful for investigating and preventing serious medical problems that can be caused by the increasing use of nanoparticle-laden drug administrations.
신학수,김정대,백구연,소광섭,권병두,고신자,안승찬,이건호 한국정신과학학회 2002 한국정신과학회 학술대회 논문집 Vol.17 No.-
일반일 5명과 기공(뇌호흡) 수련자 17명을 대상으로 손에서 방출되는 자기장을 fluxgate로 측정하였다. 4명의 대상자로부터 미약하지만 특징적인 주파수를 갖는 1nT 내외의 자기장이 측정되었고, 2명의 대상자로부터 0.1 ~ 4Hz 범위의 주파수를 갖는 100nT 정도의 강한 자기장을 측정했다. 그러나 자기장이 손에서 방출되었는지, 아니면 배경의 자기장이 손의 자성체 성질의 변화에 의해 나타나는 간접적 현상인지는 확실히 구분할 수 없었다.