Solid state fluorescence enhancement of a oxadiazole molecules for immunofluorescence labelling

배은혜,김형준 한국공업화학회 2012 한국공업화학회 연구논문 초록집 Vol.2012 No.1

Association between timing of intubation and mortality in patients with idiopathic pulmonary fibrosis

배은혜,Park Jimyung,Choi Sun Mi,이진우,Lee Sang-Min,Lee Hong Yeul 대한중환자의학회 2022 Acute and Critical Care Vol.37 No.4

Background: Delayed intubation is associated with poor prognosis in patients with respiratory failure. However, the effect of delayed intubation in patients with idiopathic pulmonary fibrosis (IPF) remains unknown. This study aimed to analyze whether timing of intubation after high-concentration oxygen therapy was associated with worse clinical outcomes in IPF patients.Methods: This retrospective propensity score-matched study enrolled adult patients with IPF who underwent mechanical ventilation between January 2011 and July 2021. Patients were divided into early and delayed intubation groups. Delayed intubation was defined as use of high-concentration oxygen therapy for at least 48 hours before tracheal intubation. The primary outcome was intensive care unit (ICU) mortality, and a conditional logistic regression model was used to evaluate the association between timing of intubation and clinical outcomes.Results: The median duration of high-concentration oxygen therapy before intubation was 0.5 days in the early intubation group (n=60) and 5.1 days in the delayed intubation group (n=36). The ICU mortality rate was 56.7% and 75% in the early and delayed intubation groups, respectively, before propensity matching (P=0.075). After matching for demographic and clinical covariates, 33 matched pairs were selected. In the propensity-matched cohort, delayed intubation significantly increased the risk of ICU mortality (adjusted odds ratio, 3.99; 95% confidence interval, 1.0215.63; P=0.046). However, in-hospital mortality did not differ significantly between the groups.Conclusions: In patients with IPF, delayed intubation after initiation of high-concentration oxygen therapy was significantly associated with increased risk of ICU mortality compared to early intubation.

Role of a Third Extracellular Domain of an Ecotropic Receptor in Moloney Murine Leukemia Virus Infection

배은혜,박성훈,정용태 한국미생물학회 2006 The journal of microbiology Vol.44 No.4

The murine ecotropic retroviral receptor has been demonstrated to function as a mouse cationic amino acid transporter 1 (mCAT1), and is comprised of multiple membranespanning domains. Feral mouse (Mus dunni) cells are not susceptible to infection by the ecotropic Moloney murine leukemia virus (MoMLV), although they can be infected by other ecotropic murine leukemia viruses, including Friend MLV and Rauscher MLV. The relative inability of MoMLV to replicate in M. dunni cells has been attributed to two amino acids (V214 and G236) located within the third extracellular loop of the M. dunni CAT1 receptor (dCAT1). Via the exchange of the third extracellular loop of the mCAT1 cDNA encoding receptor from the permissive mouse and the corresponding portion of cDNA encoding for the nonpermissive M. dunni receptor, we have identified the most critical amino acid residue, which is a glycine located at position 236 within the third extracellular loop of dCAT1. We also attempted to determine the role of the third extracellular loop of the M. dunni CAT1 receptor with regard to the formation of the syncytium. The relationship between dCAT1 and virus-induced syncytia was suggested initially by our previous identification of two MLV isolates (S82F in Moloney and S84A in Friend MLV), both of which are uniquely cytopathic in M. dunni cells. In an attempt to determine the relationship existing between dCAT1 and the virally-induced syncytia, we infected 293-dCAT1 or chimeric dCAT1 cells with the S82F pseudotype virus. The S82F pseudotype virus did not induce the formation of syncytia, but did show increased susceptibility to 293 cells expressing dCAT1. The results of our study indicate that S82F-induced syncytium formation may be the result of cell-cell fusion, but not virus-cell fusion.

마우스레트로바이러스에 의한 합포체 형성 기작 분석

배은혜,박성한,정용태,Bae, Eun-Hye,Park, Sung-Han,Jung, Yong-Tae 한국미생물학회 2006 미생물학회지 Vol.42 No.3

To study the mechanism of syncytium formation, novel syncytia-inducing ecotropic murine retrovirus was used. Our previous result showed that amino acid substitutions at the RBD (receptor binding domain) of envelope glycoprotein contribute to syncytium formation. In this study, we have investigated if this fusion phenomenon could occur with retroviral vectors pseudotyped with the novel syncytia-inducing ecotropic murine leukemia virus Env. We have found that these vectors were not able to mediate virus-to-cell fusion in M. dunni murine cell lines. These findings indicate that syncytia-inducing ecotropic murine leukemia virus is capable of generating syncytia during its replication. There was also no correlation between the level of ecotropic murine leukemia virus receptor (mCAT-1) and the fusogenic effect. 레트로바이러스의 합포체(syncytia)형성 기작 연구를 위해 합포체 형성을 유도하는 새로운 ecotropic 마우스레트로바이러스(Friend murine leukemia virus)변이주를 실험에 사용하였다. 마우스레트로바이러스의 외막에 존재하는 당단백질 중 수용체와 결합하는 부위의 아미노산을 변화시키면 합포체를 형성할 수 있음이 이미 밝혀졌다. 본 연구에서는 합포체 유도 마우스레트로바이러스 외막 당단백질을 가진 pseudotype 레트로바이러스 벡터로 부터도 이러한 융합 현상이 일어날 수 있는지 알아보았다. 마우스 세포주인 M. dunni에 pseudotype 바이러스를 감염시킨 결과 레트로바이러스 벡터 매개에 의한 바이러스-세포간 융합 현상은 일어나지 않았다. 이러한 실험결과는 합퐁체 형성이 바이러스 복제가 가능한 합포체 유도 마우스레트로바이러스에만 일어남을 나타낸다. 또한 ecotropic 마우스레트로바이러스 수용체의 농도와 막 융합과의 상관관계도 없는 것으로 밝혀졌다.

기후변화에 따른 금강 유역의 어류 종분포 변화 예측

배은혜,정진호,Bae, Eunhye,Jung, Jinho 응용생태공학회 2015 Ecology and resilient infrastructure Vol.2 No.3

기후변화에 의한 수생태계의 영향은 수생생물의 생리작용의 변화에서부터 종분포에 이르기까지 광범위할 것으로 예상된다. 본 연구에서는 기후변화에 따른 금강유역의 어류 종분포를 예측하기 위하여 비출현 정보를 요구하지 않고도 좋은 예측력을 가지고 있는 MaxEnt 모형을 활용하였다. 금강유역 134개 지점의 2007년부터 2009년까지 어류 출현 자료 (고유종 17종 포함 총 47종)와 9개의 환경인자 (월별 최저기온, 최고기온, 평균기온, 강수량, 최저수온, 최고수온, 평균수온, 고도, 경사)를 사용하여, RCP 8.5 기후변화 시나리오에 따라 2050년과 2100년의 어류 종분포를 예측하였다. MaxEnt 모형은 평균 0.798의 적절한 모형적합도를 보여 주었으며, 종분포 예측에 기여도가 높은 환경인자는 고도, 강수량, 최저수온 순이었다. 기후변화가 진행됨에 따라 얼룩동사리와 줄납자루와 같은 고유종의 출현확률은 감소한 반면, 배스와 블루길과 같은 외래종의 출현확률은 증가하였다. 특히 2100년에는 5종 (줄몰개, 미꾸라지, 강준치, 줄납자루, 칼납자루)의 어류가 금강유역에서 더 이상 서식하지 못하는 것으로 예측되었다. 그리고 기후변화에 따라 종풍부도가 높은 지역이 금강유역 내에서 북상하는 것으로 예측되었다. 이러한 결과는 기후변화로 인한 수온 상승이 금강유역 수생태계의 교란을 심화시킬 수 있다는 것을 의미한다. Impacts of climate change on aquatic ecosystems range from changes in physiological processes of aquatic organisms to species distribution. In this study, MaxEnt that has high prediction power without nonoccurrence data was used to simulate fish distribution changes in the Geum river watershed according to climate change. The fish distribution in 2050 and 2100 was predicted with RCP 8.5 climate change scenario using fish occurrence data (a total of 47 species, including 17 endemic species) from 2007 to 2009 at 134 survey points and 9 environmental variables (monthly lowest, highest and average air temperature, monthly precipitation, monthly lowest, highest and average water temperature, altitude and slope). The fitness of MaxEnt modeling was successful with the area under the relative operating characteristic curve (AUC) of 0.798, and environmental variables that showed a high level of prediction were as follows: altitude, monthly average precipitation and monthly lowest water temperature. As climate change proceeds until 2100, the probability of occurrence for Odontobutis interrupta and Acheilognathus yamatsuatea (endemic species) decreases whereas the probability of occurrence for Microphysogobio yaluensis and Lepomis macrochirus (exotic species) increases. In particular, five fish species (Gnathopogon strigatus, Misgurnus mizolepis, Erythroculter erythropterus, A. yamatsuatea and A. koreensis) were expected to become extinct in the Geum river watershed in 2100. In addition, the species rich area was expected to move to the northern part of the Geum river watershed. These findings suggest that water temperature increase caused by climate change may disturb the aquatic ecosystem of Geum river watershed significantly.

『훈민정음』 해례본의 외형 복원을 위한 침안(針眼) 재구성

배은혜,백두현 국어사학회 2020 국어사연구 Vol.0 No.30

『훈민정음』 간송본은 오백여 년 동안 보존되어 온 과정에서 여러 차례 재장정되었 다. 그 결과 선장하면서 실 노끈으로 꿰맨 침안(針眼)의 양상이 복잡해져서 원래의 침 안을 구성해 내기 어려운 상태이다. 새로 발견된 상주본은 훼손이 심하고 모두 공개 되지 않아 침안을 분석하기 어렵다. 이 글에서 우리는 최초 장정 당시의 침안을 추정하기 위해, 간송본의 침안을 자세 히 분석하고, 두 해례본의 침안을 비교해 보았다. 두 해례본의 여러 판면을 분석하여 장정의 흔적을 살펴본 결과, 간송본에는 4침안 두 종류와 5침안 한 종류라는 세 가지 침안 유형이 있음을 밝혔고, 상주본에는 5침안 한 종류가 있음을 확인하였다. 간송본 과 상주본은 동일한 판본에서 쇄출된 것이나 침안 구성을 비교 분석해 본 결과, 간행 당시에 두 해례본의 침안 위치는 서로 달랐음을 밝혔다. 아울러 해례본 간행 당시의 다른 문헌에 나타난 침안을 해례본과 비교하여 5침안이 일반적인 것임을 확인하였다. 우리는 해례본 간행 당시의 침안을 5침안으로 추정하였다. 간송본은 여러 차례 개 장되는 과정에서 ‘5침안→ 4침안B형→ 4침안A형’으로 변화한 것이라고 판단하였다. 이렇게 판단한 근거로 두 해례본에 5침안이 공통적으로 나타나는 사실, 5침안정법은 우리나라 선장본 문헌의 주요 양식이라는 점, 해례본이 최초 장정되었던 15세기 간행 문헌의 경우 거의 대부분이 5침안의 선장본이라는 사실, 4침안 장정은 조선 말기의 일부 문헌에서 발견된다는 사실 등을 제시하였다. 본고의 연구 결과는 훈민정음 해례 본의 외형을 최초 간행 당시의 모습으로 복원하는 데 기여할 수 있을 것이다. The Kansong copy of Hunminjeongeum displays many traces of rebinding that occurred over hundreds of years of preservation, thereby showing a complex network of stitching holes. On the other hand, the damaged state of the Sangju copy makes it hard to distinguish its binding holes. After analyzing the various wooden plates of Haeryebon and examining the binding marks, the Kansong copy appears to bear three patterns of binding, which consist of two 4-hole binding types and one 5-hole binding type, while the Sangju copy shows only a 5-hole binding type. In order to estimate which binding was the original type, the bindings of the two copies were compared, in addition to which the binding patterns of ancient manuscripts other than Haeryebon were e x amined. As a r esult, the two Haeryebon copies appear to be printed on the same woodblock printed sheets, but the positions of the stitching holes were changed at the time of binding the books. This paper argues that the original binding pattern of Haeryebon is the 5-hole binding type and therefore the rebinding process of the Kansong copy must have been as follows:‘5-hole →4hole (type B) →4-hole (type A)’. On this basis, this study (1) presents the similarities of the 5-hole patterns between the two Haeryebon copies; (2) argues that the 5-hole binding pattern is characteristic of the Korean cord-bound books (seonjangbon); (3) shows that in the 15th century when Haeryebon was first bound, most of the ancient books were 5-hole cord-bound books (with the exception of some wrapped-back books (pobaejangbon)); and (4) suggests that the 4-hole binding type can be found in some old manuscripts of late Joseon. These findings may help towards the restoration of the Hunminjeongeum Haeryebon into its original form.

박광선의 친필본『보덕공 비망록』한글 자료 연구

배은혜,안미애 언어과학회 2022 언어과학연구 Vol.- No.100

Bodeokgong Bimangnok is a miscellaneous manuscript, which includes both Hanja and Hangul texts and was written by Park Kwangseon (1569~1631) of Goryeong from 1605 to 1623. This study focuses on the texts written in Hangul to examine what they can tell us about the history of the Korean language. A particularly noteworthy feature of this manuscript is that it contains the first hypercorrected form ‘김ᄎᆡ’ (kimchi) instead of ‘짐ᄎᆡ’. While the manuscript does not contain as many Hangul texts as it does in Classical Chinese, the notation and phonological characteristics it reveals do reflect the language of the 17th-century Gyeongsang-do province and thus provide invaluable insights into the history of the Korean language, especially when compared with Hyeonpungkwakssieongan, a contemporary manuscript from a neighboring region. .

Preoperative risk evaluation and perioperative management of patients with obstructive sleep apnea: a narrative review

배은혜 대한치과마취과학회 2023 Journal of Dental Anesthesia and Pain Medicine Vol.23 No.4

Obstructive sleep apnea (OSA) is a common sleep-breathing disorder associated with significant comorbidities and perioperative complications. This narrative review is aimed at comprehensively overviewing preoperative risk evaluation and perioperative management strategies for patients with OSA. OSA is characterized by recurrent episodes of upper airway obstruction during sleep leading to hypoxemia and arousal. Anatomical features, such as upper airway narrowing and obesity, contribute to the development of OSA. OSA can be diagnosed based on polysomnography findings, and positive airway pressure therapy is the mainstay of treatment. However, alternative therapies, such as oral appliances or upper airway surgery, can be considered for patients with intolerance. Patients with OSA face perioperative challenges due to difficult airway management, comorbidities, and effects of sedatives and analgesics. Anatomical changes, reduced upper airway muscle tone, and obesity increase the risks of airway obstruction, and difficulties in intubation and mask ventilation. OSA-related comorbidities, such as cardiovascular and respiratory disorders, further increase perioperative risks. Sedatives and opioids can exacerbate respiratory depression and compromise airway patency. Therefore, careful consideration of alternative pain management options is necessary. Although the association between OSA and postoperative mortality remains controversial, concerns exist regarding adverse outcomes in patients with OSA. Understanding the pathophysiology of OSA, implementing appropriate preoperative evaluations, and tailoring perioperative management strategies are vital to ensure patient safety and optimize surgical outcomes.

A Case of Anti-tuberculous Drug-induced Liver Injury in the Liver Transplant Recipient

배은혜,곽낙원 대한내과학회 2020 대한내과학회 추계학술대회 Vol.2020 No.1

내한 특성을 가지는 표면처리용 수분산 코팅 수지 합성 및 특성 연구

배은혜,김은지 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2

최근 들어 극지 운항 선박이나 특수선에 사용되는 케이블에 대한 고내한성 요구 수요가 증가하고 있으나 열, 유증기, 오존 등에 대한 저항력을 부여하는 기술적 난이도가 높아 현재까지 어려움을 겪고 있는 실정이다. 케이블은 철도차량, 선박, 자동차 등 사용되는 환경에 따라 내한성, 내유성, 내화학성, 내약 품성 등이 동시에 요구되며, 기존의 케이블은 영하 50 ℃의 내한 성능을 요구하였으나 극지 운항 선박 등에 사용되는 케이블은 최대 영하 60 ℃ 수준에서 유연성을 유지하는 고내한성이 요구되고 있다. 따라서, 본 연구에서는 케이블의 고내한성 부여를 위해 케이블 내 사용되는 소재사에 적용할 수 있는 저온 특성을 가진 표면 처리용 코팅 수지를 합성하고 그 특성을 평가하였다. *본 연구는 산업통상자원부와 산업기술평가관리원이 지원하는 “소재부품기술개발-전략핵심소재자립화기술개발사업”으로 수행된 연구결과입니다(과제번호 : 20013166).