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쪽(Polygonum tinctortium) 세포의 형질전환 및 쪽 세포에서 Sodium Butyrate가 Green Fluorescent Protein 발현에 미치는 영향
박성길,정충식,이종진,이윤형,정인식,Park, Sung-Kil,Chung, Choong-Sik,Lee, Jong-Jin,Lee, Youn-Hyung,Chung, In-Sik 한국응용생명화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4
쪽(Polygonum tinctortium) 세포에서 외래 단백질 발현을 검토하기 위하여 green fluorescent protein(GFP)가 내재하는 pCAMBIA1302를 형질 전환시켰으며 Western blot 분석에 의해 GFP의 발현을 확인하였다. Sodium butyrate가 GFP생성에 미치는 효과를 검토한 결과, 10 mM에서 세포성장이 지연되었으며, 15 mM 이상에서는 정지되었다. 세포 내 GFP 생성량은 세포 접종 후 3일째 5 mM sodium butyrate를 첨가하였을 때가 0일째 처리에 비해 120% 증가하였다. 또한 접종후 3일 후 5 mM의 sodium butyrate를 처리한 경우가 10 mM의 경우보다 GFP의 수율이 50% 증가하였다. 본 실험을 통하여 세포 접종 후 3일째, 5 mM의 농도로 처리한 sodium butyrate가 외래 단백질의 발현을 효과적으로 증가시키는 결과를 확인하였다. To examine the expression of foreign protein in Polygonum tinctorium cells, plasmid pCAMBIA1302 encoding Green Fluorescent Protein(GFP) was used to transform the cells and the expression was confirmed using Western blot analysis. When the effect of sodium buryrate on the formation of GFP was examined, cell growth was retarded at the addition of 10 mM and was stalled at more than 15 mM. The amount of GFP production was increased by 15% when 5 mM of sodium butyrate was added at three-days after inoculation as compared to at 0-day. Moreover, when sodium butyrate was added at three-days after inoculation, the amount of GFP was increased by 50% at the addition of 5 mM of sodium butyrate as compared to 10 mM.
형질전환 담배 식물체에서 개똥쑥 Terpene Synthase의 발현
박성길,홍성현,최근원,이윤형,정인식 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-
Expression of Artemisia annua terpene synthase in transgenic tobacco plants was examined. A full length cDNA encoding terpene synthase was cloned into pILTAB357 vector and transgenic tobacco plants were generated. The integration of this gene was analyzed by genomic DNA PCR and Southern blot analysis. The transcription of the terpene synthase gene in tobacco was confirmed by RT-PCR and northern blot analysis.
박성길,김창대 한국청소년상담복지개발원 2003 청소년상담연구 Vol.11 No.1
본 연구는 최근 청소년들에게 문제로 부각되는 인터넷 과다사용1)의 ‘위험요소2) (risk factor)를 탐색적으로 밝히고 자 하는 것이다. ’중독‘ 과 ’원인‘이라는 개념을 ’과다사용‘과 ’위험요소‘라는 개념으로 대체함으로써 엄밀성을 더하 고 기존의 연구에서 주로 사용되었던 질문지법의 한계를 극복하기 위해 실제 사이버상담의 내용을 분석하였다. 연구자는 표집된 사이버상담 사이트에서 발췌한 80 사례를 객관적으로 선정하고, 위험요소와 관련된 서술을 최소 의미단락으로 나누어 최종적으로 129 건을 발췌하여 분석의 대상으로 삼았다. 분석결과 개인요인으로는 성격적 특 성, 대인관계 문제, 보상경험이 있었고 인터넷 관련 요인으로는, 컨텐츠 자체의 매력, 컴퓨터 관련 취미, 다른 취미 생활의 통로로 나타났다. 주변환경 관련 요인으로는 또래문화, 접근용이성 증가, 고위험상황, 인터넷매개 학습, 가 족문제가 발견되었다. 본 연구의 의의는 청소년의 인터넷 과다사용 상태를 초래할 수 있는 여러 위험요소들이 실제의 사례를 통해서 확 인된 것이다. 특히 청소년의 인터넷 과다사용에는 환경적인 위험요소의 영향력이 상대적으로 큰 것으로 보아 현재 의 예방프로그램들은 환경적인 접근방식도 충분히 고려하는 방향으로 변화 발전되어야 할 것이다. The purposes of this study were to identify risk factors that may lead adolescents to the excessive use of internet. The main method used in this study was content analysis and the process was as follows. First, four internet sites for internet addiction counseling were chosen through systematic process. Second, 80 cyber-counseling cases were collected from those sites. Third, all the cases were analyzed by content analysis method. Fourth, criterion for classification constructed by 2 co-researchers through Q-sort. Fifth, 129 paragraphs were classified on the basis of categories in the previous step. The results are as follows: Three major risk factor groups were found. Those are environment-related risk factor group, internet-related risk factor group and individual-related risk factor group. Total 11 risk factors were identified in the three major risk factor groups. Peer culture, increase of accessability, high risk situation, web-based instruction and family problem were included in environment-related risk factor group. Attraction of internet contents, computer related hobbies and other hobbies using internet were included in internet-related risk factor groups. Finally the prominent contributed to excessiveness of internet personality, interpersonal skill and compensation experience were included in individual related risk factor groups. This study showed that the environment-related risk factor group was most notable. Therefore, environment-related risk factors must be seriously considered in planning programs for adolescents. It was also suggested that parent education program be prepared and implemented to discipline adolescents who used internet excessively