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잘피 게바다말로부터 폴리페놀 물질(MP-1)의 분리 및 동정
김해선,박년호,석호영,유상권,우정희 한국환경농학회 2022 한국환경농학회지 Vol.41 No.1
BACKGROUND: Phyllospadix japonica Makino is a perennial plant belonging to the family Zosteraceae. This species is native to the eastern coast of the Korean Peninsula, and it is found attached to rocks on the seashore. As with all seagrass species, P. japonica is also known to play a major role in protecting the marine envi-ronment, and studies on its physiological activities sug-gestive of its antioxidant, antibacterial and anti-in-flammatory potentials have been reported. In this study, purification and structural analysis were performed to identify the polyphenol substances derived from P. japonica. METHODS AND RESULTS: An polyphenol substance MP-1 was purified from the 70% aqueous methanol extract of P. japonica using Diaion® HP-20 column chro-matography, ODS column chromatography, and medium- pressure liquid chromatography (MPLC). The purified MP-1 was identified as rosmarinic acid having a molec-ular weight of 360 and a molecular formula of C18H16O8 through electrospray ionization (ESI)-mass and nuclear magnetic resonance (NMR) spectroscopic analysis. CONCLUSION(S): This study highlights the processes used for the identification of the polyphenol substance de-rived from P. japonica. Rosmarinic acid, the polyphenol derived from P. japonica identified by this study, is a kind of bioactive substance mainly present in plants. These findings provide an important starting point and are val-uable for future studies on bioactive substances in seagrass.
우정희,강경명,권태형,박년호,이은열 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.28 No.-
Marine bacteria possessing epoxide hydrolase (EH) activity were isolated from the oil-spilledforeshore of South Korea. While the isolated Rhodococcus sp. YSMI04 and YSNA32 showed differentenantioselectivities to racemic styrene oxide (SO), two strains exhibited same enantiopreference to3-chlorostyrene oxide (3-CSO) and 4-CSO. In the case of 3-CSO, Rhodococcus sp. YSMI04 and YSNA32exhibited (R)-3-CSO preferred hydrolysis activity, whereas Roseobacter sp. TSBP12 showed a preference for(S)-3-CSO. In the case of 4-CSO, Rhodococcus sp. YSMI04 and YSNA32 showed enantiopreference to(S)-4-CSO, Roseobacter sp. TSBP12 exhibited (R)-4-CSO preferred activity. (S)-4-CSO was obtainedwith 35% yield (theoretically 50%) using Roseobacter sp. TSBP12
Draft genome sequence of Roseobacter sp. TSBP12 containing oxygenase activity to degrade indole
김해선,차선호,석호영,박년호,우정희 한국미생물학회 2020 미생물학회지 Vol.56 No.1
Roseobacter sp. TSBP12, a polyaromatic hydrocarbon (PAH) degrading bacterium, was isolated from an oil-contaminated sediment sample. This strain has been known to contain a strong oxidative/degradative activity of indole to produce indigo. In this study, the genomic structure of this strain was characterized using Ion Torrent PGM platform for the first time. A total of 96 contigs were generated, and the genome size was predicted to be 4,393,160 bp with the G + C content of 58.0%. Our analysis also revealed that this genome contained multiple oxygenases, including two subunits (a and b) of aromatic-ring hydroxylating dioxygenase and a flavin containing monooxygenase (FMO) that are known to be involved in the bioconversion of indigo from indole.
김종식,우정희,김준태,박년호,김충곤,Kim, Jong-Shik,Woo, Jung-Hee,Kim, Jun-Tae,Park, Nyun-Ho,Kim, Choong-Gon 한국미생물학회 2010 미생물학회지 Vol.46 No.1
미생물 군집과 기능연구를 위한 최근의 새로운 분석기술의 발전은 다양한 유전 관련 정보를 제공해왔다. mRNA를 포함하는 핵산을 기초로 한 연구를 뛰어넘어서 메타프로테오믹스는 미생물 군집의 유전형 및 표현형의 특징적인 정보를 보다 정교하게 제공할 수 있다. 이미 서로 다른 미생물 생태계인 해수, 인간의 배설물, 활성 슬러지, 산성 광산 폐수 생물막, 토양 등에 메타프로테오믹스 기술이 유용하게 사용되었다. 이들 연구는 여러 측면에서 상당히 다르지만 미생물 군집의 구조, 기능, 생리, 상호관계, 생태, 진화적 측면을 결정적으로 상호 연결한다는 것을 밝혀냈다. 본 총설은 메타프로테오믹스에 대한 현재까지의 가장 최신의 정보를 요약하여 제공함으로써 메타프로테오믹스에 대한 정확한 이해와 활용을 통해 다방면의 메타프로테오믹스가 가능하도록 하고자 하였다. New technologies are providing unprecedented knowledge into microbial community structure and functions. Even though nucleic acid based approaches provide a lot of information, metaproteomics could provide a high-resolution representation of genotypic and phenotypic traits of distinct microbial communities. Analyzing the metagenome from different microbial ecosystems, metaproteomics has been applied to seawater, human guts, activated sludge, acid mine drainage biofilm, and soil. Although these studies employed different approaches, they elucidated that metaproteomics could provide a link among microbial community structure, function, physiology, interaction, ecology, and evolution. These approaches are reviewed here to help gain insights into the function of microbial community in ecosystems.
김해선,차선호,석호영,박년호,우정희,Kim, Hae-Seon,Cha, Sun Ho,Suk, Ho Young,Park, Nyun-Ho,Woo, Jung-Hee The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3
Yangia sp. TSBP01, isolated from tidal flat sediment contaminated by the oil spill, is known to convert indole to indigo via an intermediate called indoxyl. Our analysis revealed that Yangia sp. TSBP01 contained the genome of 5,165,974 bp (G + C content: 66.5%) being composed of two chromosomes and five plasmids. This strain had genes encoding several oxygenases such as indole oxygenase directly involved in the conversion of indole to indoxyl. 유류 누출 사고로 오염된 해양 갯벌에서 분리된 Yangia sp. TSB01은 인돌을 인디고로 생물전환 할 수 있는 특성이 있다. 미생물에서 인디고는 산화효소에 의해 인돌로부터 인독실을 거쳐 생성된다. Yangia sp. TSBP01의 유전체는 2개의 chromosome과 5개의 plasmid로 구성되어 있으며, 총 5,165,974 bp의 크기에 G + C 함량은 66.5%이다. TSBP01는 인디고를 생성하는데 관여하는 indole oxygenase를 보유하고 있다.