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김해선,차선호,석호영,박년호,우정희,Kim, Hae-Seon,Cha, Sun Ho,Suk, Ho Young,Park, Nyun-Ho,Woo, Jung-Hee The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3
유류 오염된 해양 갯벌에서 분리한 다환방향수소족(PAHs)을 분해하는 균주들로부터 인디고로 생물전환 활성을 가진 것으로 예측되는 Azoarcus sp. TSPY31과 TSNA42 균주를 동정하였다. 이 두 균주의 유전체 분석을 실시한 결과, 모두 하나의 완전한 chromosome으로 구성되며, TSPY31은 총 4,572,082 bp에 G + C 함량은 63.2%로 이루어져 있고, TSNA42는 4,886,934 bp에 G + C 함량은 62.8%이었다. 이 두 균주 모두 인돌을 인디고로 전환하는 효소인 styrene monooxygenase를 각각 2 copy씩 보유하고 있는 것으로 확인되었다. Azoarcus are known to contain bacterial strains usually found in contaminated areas. Two strains of Azoarcus sp., TSPY31 and TSNA42, were isolated from oil-contaminated marine tidal flats, and their genomic structures were analyzed. The genomes of both TSPY31 and TSNA42 were composed of a single complete chromosome of 4,572,082 bp (G + C content: 63.2%) and 4,886,934 bp (G + C content: 62.8%), respectively. Both genomes were found to contain two copies of styrene monooxygenases that are predicted to be responsible for converting indole to indigo.
김해선,차선호,석호영,박년호,우정희,Kim, Hae-Seon,Cha, Sun Ho,Suk, Ho Young,Park, Nyun-Ho,Woo, Jung-Hee The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3
Yangia sp. TSBP01, isolated from tidal flat sediment contaminated by the oil spill, is known to convert indole to indigo via an intermediate called indoxyl. Our analysis revealed that Yangia sp. TSBP01 contained the genome of 5,165,974 bp (G + C content: 66.5%) being composed of two chromosomes and five plasmids. This strain had genes encoding several oxygenases such as indole oxygenase directly involved in the conversion of indole to indoxyl. 유류 누출 사고로 오염된 해양 갯벌에서 분리된 Yangia sp. TSB01은 인돌을 인디고로 생물전환 할 수 있는 특성이 있다. 미생물에서 인디고는 산화효소에 의해 인돌로부터 인독실을 거쳐 생성된다. Yangia sp. TSBP01의 유전체는 2개의 chromosome과 5개의 plasmid로 구성되어 있으며, 총 5,165,974 bp의 크기에 G + C 함량은 66.5%이다. TSBP01는 인디고를 생성하는데 관여하는 indole oxygenase를 보유하고 있다.
Draft genome sequence of Roseobacter sp. TSBP12 containing oxygenase activity to degrade indole
김해선,차선호,석호영,박년호,우정희 한국미생물학회 2020 미생물학회지 Vol.56 No.1
Roseobacter sp. TSBP12, a polyaromatic hydrocarbon (PAH) degrading bacterium, was isolated from an oil-contaminated sediment sample. This strain has been known to contain a strong oxidative/degradative activity of indole to produce indigo. In this study, the genomic structure of this strain was characterized using Ion Torrent PGM platform for the first time. A total of 96 contigs were generated, and the genome size was predicted to be 4,393,160 bp with the G + C content of 58.0%. Our analysis also revealed that this genome contained multiple oxygenases, including two subunits (a and b) of aromatic-ring hydroxylating dioxygenase and a flavin containing monooxygenase (FMO) that are known to be involved in the bioconversion of indigo from indole.
한국산 도롱뇽(Hynobius leechii)염색체의 인형부위(NORs)다양성에 관하여
이혜영,차선호 한국유전학회 1995 Genes & Genomics Vol.17 No.2
The chromosome constitutions of 106 individuals of Hynobius Ieechii collected from 17 localities in Korea were analyzed by Giemsa and Ag-NOR staining methods. The chromosome number of most of the individuals was 56 with AN number of 94. The size of NORs was homomorphic or heteromorphic pair at the tip of centromere of No. 23 chromosomes in 59 out of 106 individuals. The rest exhibited a high level of variation in number and site of NORs, but there was no intra-individual variation. All specimens from 4 (Hadong, Kurye, Chisan, Yangpyong) out of 17 populations had NORs on No. 23 chromosome, while many specimens from the rest of the populations had NORs variations with the rate of 44%. The mechanisms of NORs variation were explained by translocation and pericentric inversion of the parts of NORs, insertion of amplified 18S+28S rRNA gene segment and Ag-NOR staining effect that stain only real NORs in a species. Especially, all the specimens from Yangsan showed that the site of NORs was located at a little below the centromere on No. 23 chromosome, which caused by pericentric inversion.
반려동물 사료의 Salmonella spp. 신속검출을 위한 증균배양법, 면역학적 검출법 및 종 특이 프라이머를 이용한 PCR 방법 비교
윤혜정,차선호,이승화,정민희,나태웅,김혜진,조현정,홍성희 한국식품위생안전성학회 2022 한국식품위생안전성학회지 Vol.37 No.1
The purpose of this study was to compare the conventional culture method, enzyme immunemethod and the PCR method using species-specific primer in the analysis on the Salmonella spp. found in domesticallydistributed pet foods. For the study, Salmonella spp. were detected from 175 samples. From the conventionalculture method and the PCR method, two samples (jerky and corn gluten) were determined as positive. Also, from theenzyme immune method, one sample (corn gluten) was test-positive. The study revealed that application of the PCR methodwith species-specific primer allows better distinguishment between the species of the strain collected from the samples than theconventional culture method and/or the enzyme immune method. 국내 유통되는 반려동물 사료의 살모넬라 분석시 증균배양법, 효소면역기법에 의한 분석, 종 특이 primer를 활용한 PCR 방법을 활용하여 비교 평가하였다. 시료 175점Salmonella spp. 검출 결과 증균배양법 및 종 특이 primer를활용한 PCR 방법에 의한 검출 방법에서 2점의 시료(육포,옥수수 글루텐)가 양성으로 확인되었고, 효소면역기법에의한 검출방법에서는 1점의 시료(옥수수 글루텐)가 양성으로 확인되었다. 증균배양법 및 효소면역기법에 의한 검출방법에 비해 종 특이 primer를 활용한 PCR 방법을 적용 할 경우 시료에서 분리된 균주의 종(species) 판별이 가능하였다.