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In Vitro Maturation of Porcine Oocytes in a Dry Incubator without CO2 Gas Supplement
박광욱 사단법인 한국동물생명공학회 2012 Reproductive & developmental biology Vol.36 No.3
The present study was conducted to develop a simple method for porcine oocyte maturation without CO2 regulation. In experiment 1, we evaluated that the effect of CO2 non-supplement on porcine oocyte maturation. Cumulusoocyte complexes (COCs) were collected from 2~6 mm follicles and divided into three groups (Control, tube-CO2,and tube-non-CO2). For control, COCs were cultured in 4-well multidish in a CO2 incubator. For tube-CO2, COCs were cultured in a round-bottom tube in a CO2 incubator, and for tube-non-CO2, COCs were cultured in a round-bottom tube sealed tightly without CO2 supplement in a dry incubator. The proportion of oocytes reached to metaphase II (M-II) was not significantly different among three groups (87.9% to 91.4%). In experiment 2, we evaluated the effect of CO2 non-supplement during oocyte maturation on development of embryos. Oocytes with a polar body were divided into two groups (Control and tube-non-CO2) and applied 1.1 kV/cm or 1.2 kV/cm voltages for parthenogenetic activation. After activation, embryos were cultured for 6 days and examined the development. The proportion of embryos cleaved was not significantly different among treatment (86.3% to 91.5%). The proportion of embryo reached to blastocyst stage was not significantly different among treatment (13.9% to 25.2%). The cell number of blastocysts was not significantly different among treatment (29.0 to 32.4). In conclusion, oocytes cultured in a dry incubator without CO2 supplement have enough competence to development after parthenogenetic activation. These results would be useful for transporting oocytes or embryos a long distance.
Porcine Oocytes with Meiotic Competence are Synchronized Early Stage of Germinal Vesicle (GV)
박광욱 사단법인 한국동물생명공학회 2010 Reproductive & developmental biology Vol.34 No.3
Correlations between cumulus cells and germinal vesicle (GV) chromatin configuration were examined in porcine oocytes. Cumulus-oocyte complexes (COCs) were collected from 2~6 mm follicles and divided into three categories according to cumulus cell morphology. "A" group was compacted COCs with more than three cumulus cell layers. "B" group was COCs with less cumulus cell layers than "A" group. "C" group was COCs with one or less layer of cumulus cells. Cumulus cells were removed 0.1% hyaluronidase, and denuded oocytes were stained with Hoechst 33342. GV chromatin configuration was classified into GV-Con and GV-Dis. GV-Con meant that a nucleus was surrounded by condensed chromatin in a ring. GV-Dis meant that filamentous chromatin clumps were distributed in nucleus. The proportion (80.2%) of GV-Con in "A" group was significantly higher than "B" (62.0%) or "C" (44.9%). The proportion (55.1%) of GV-Dis in "C" group was significantly higher than "A" (19.8%) or "B" (38.0%). The meiotic competence of COCs was examined after 44 h culture. The proportion (90.0%) of oocytes reaching to metaphase II (M-II) in "A" group was significantly higher than "B" (76.5%) or "C" (45.5%). In conclusion, oocytes with good quality cumulus cell layers are synchronized early GV stage, and early GV stage is important for meiotic competence in pigs.
박광욱,최성식,이동호,이황,최성규,박장식,이상호 사단법인 한국동물생명공학회 2011 Reproductive & developmental biology Vol.35 No.1
wo piglets and one juvenile pig were used to investigate closely what types of cells express green fluorescent protein (GFP) and if any, whether the GFP-tagged cells could be used for stem cell transplantation research as a middle-sized animal model in bone marrow cells of recloned GFP pigs. Bone marrow cells were recovered from the tibia, and further analyzed with various cell lineage markers to determine which cell lineage is concurrently expressing visible GFP in each individual animal. In the three animals, visible GFP were observed only in proportions of the plated cells immediately after collection, showing 41, 2 and 91% of bone marrow cells in clones #1, 2 and 3, respectively. The intensity of the visible GFP expression was variable even in an individual clone depending on cell sizes and types. The overall intensities of GFP expression were also different among the individual clones from very weak, weak to strong. Upon culture for 14 days in vitro (14DIV), some cell types showed intensive GFP expression throughout the cells; in particular, in cytoskeletons and the nucleus, on the other hand. Others are shown to be diffused GFP expression patterns only in the cytoplasm. Finally, characterization of stem cell lineage markers was carried out only in the clone #3 who showed intensive GFP expression. SSEA-1, SSEA-3, CD34, nestin and GFAP were expressed in proportions of the GFP expressing cells, but not all of them, suggesting that GFP expression occur in various cell lineages. These results indicate that targeted insertion of GFP gene should be pursued as in mouse approach to be useful for stem cell research. Furthermore, cell- or tissue-specific promoter should also be used if GFP pig is going to be meaningful for a model for stem cell transplantation.
이차원 QR Code에서 데이터 코드워드의 디코딩 알고리즘
박광욱,이종연 한국융합학회 2013 한국융합학회논문지 Vol.4 No.4
2차원 QR Code는 1차원 바코드의 용량, 크기, 방향에 대한 한계를 개선하였으며, 방대한량의 데이터를 압축하여 저장할 수 있는 능력을 갖추고 많은 응용분야에서 사용되고 있다. 최근에는 QR Code를 인식할 수 있는 스마트 폰의 도입과 사용의 증대로 QR Code의 도입이 급속도로 확산되었다. 하지만 다양한 정보를 저장할 수 있는 QR Code의 디코딩 기술에 대한 구체적인 문서화가 없는 상태이다. 따라서 본 논문에서는 QR code의 인코딩에 따른 디코딩 과정에 대해 구체적인 처리 절차와 알고리즘을 제시하고 예를 통해 디코딩 과정을 상세히 이해시키는 데 연구목적이 있다.
박광욱,이종연,Park, Kwang Wook,Lee, Jong Yun 한국디지털정책학회 2015 디지털융복합연구 Vol.13 No.2
The two-dimensional QR code has advantages such as directional nature, enough data storage capacity, ability of error correction, and ability of data restoration. There are two major issues like speed and correctiveness of recognition in the two-dimensional QR code. Therefore, this paper proposes a morphology-based algorithm of detecting the interest region of a barcode. Our research contents can be summarized as follows. First, the interest region of a barcode image was detected by close operations in morphology. Second, after that, the boundary of the barcode are detected by intersecting four cross line outside in a code. Three, the projected image is then rectified into a two-dimensional barcode in a square shape by the reverse-perspective transform. In result, it shows that our detection and recognition rates for the barcode image is also 97.20% and 94.80%, respectively and that outperforms than previous methods in various illumination and distorted image environments. 2차원 QR 코드는 1차원 바코드의 데이터 용량 문제를 극복하였고, 방향성, 오류 정정, 데이터 복원력 등의 장점이 있다. 특히 2차원 바코드 인식에서 주요 이슈는 인식 속도와 정확성이다. 따라서 본 논문에서는 바코드 영역을 검출하기 위한 알고리즘을 제안하며, 제안 방법은 영상 내 관심 영역의 위치를 검출하기 위해 모폴로지 기법을 기반으로 한다. 세부적인 연구내용은 다음과 같다. 첫째, 모폴로지 닫힘(close) 연산을 통해 입력 이미지에서 QR Code의 바코드 영역을 검출한다. 둘째, 경계선 검출을 통해 바코드 영역의 외곽선들을 검출한다. 셋째, 검출된 네 개의 외곽 교차점인 네 점을 추출한 후 역 투시변환을 통하여 2차원 바코드의 정사각형 모양으로 정규화 한다. 결과적으로 본 논문의 연구결과는 다양한 조명상태이나 영상에 강한 왜곡이 있는 경우에도 좋은 성능을 나타내며, 영역 검출율은 94.8%, 인식률은 92.3%로 기존연구들보다 안정된 바코드 검출 및 인식 성능을 보여주고 있다.