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계피(Cinnamomum zeylanicum) 정유의 유제 및 입제의 잉어(Cyprinus carpio) 및 제브라피쉬에 대한 생태독성
남태훈,전황주,김경남,김형미,김용찬,이성은 한국응용생명화학회 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.2
In this study, cinnamon essential oil (CEO) was formulated as emulsifiable concentrate (EC) and a granule. For the evaluation of their negative effects on the ecosystem, acute toxicities against Cyprinus carpio was determined in a static condition. The formulations were made using CEOs extracted by 3 different methods (steam distillation (SD), solvent extraction and supercritical fluid extraction (SFE)) and were tested to obtain LC50 values. Among the ECs, EC including CEO extracted by SFE showed highest acute toxicity against C. carpio. Among the granules, a granule including CEO extracted by SD showed highest acute toxicity against C. carpio. Nevertheless, LC50 of EC and a granule formulation with CEOs was higher than toxicity level III of pesticide standardized by Korea rural development administration. These results were similar to those using zebrafishes. Chronic toxicities were not found for 45 days in zebrafishes until 500 μgL−1 level of EC formulation including CEO obtained by the SD. Based on these results, EC formulation of CEOs may be considered to be used as environmental-friendly natural insecticides in accordance with the standards. 계피정유를 유제 및 입제로 제형화한 후 이들의 생태독성을 측정하였다. 잉어를 이용한 급성독성에 사용된 계피정유는 수증기증류법, 핵산을 이용한 용매추출법 및 초임계 추출법을 이용하여 얻었고 이들의 유제 및 입제의 생태독성학적 영향은 반수치사농도(LC50)를 구하여 평가하였다. 유제 중 초임계추출법으로얻은 정유를 함유한 제형이 잉어에 대하여 가장 높은 독성을나타내었다. 입제 중 수증기 증류법으로 추출한 계피정유를 함유한 입제가 잉어에 대하여 가장 높은 급성독성을 보였다. 이러한 결과는 3급 어독성에 해당되는 것으로 환경 중 사용에는 문제가 없다고 사료된다. 잉어를 이용하여 얻은 급성독성 결과와 같이 제브라피쉬를 사용하여 얻은 급성독성 결과 값들은 유사하였다. 수증기증류법으로 얻은 계피정유를 함유한 유제에 노출된 제브라피쉬를 이용한 만성독성은 45일 동안 500 mg L−1 수준에서 독성이 관측되지 않았다. 따라서, 계피정유의 유제 및입제의 급만성 어독성은 친환경제재로 사용하기에 적합한 것으로 사료된다
남태훈,윤정현,최재일,이성은 한국환경생물학회 2017 환경생물 : 환경생물학회지 Vol.35 No.2
To determine their acute toxicities on the earthworm (Eisenia fetida), six toxic chemicals were evaluated, according to the OECD guideline 207: sulfuric acid, methanol, methylethylketone, nitric acid, formic acid, and toluene. Sulfuric acid exhibited the maximum toxicity. The LC50 values of sulfuric acid, nitric acid, formic acid, and toluene were 20.5, 49.1, 55.5, and 534.5 μg cm-2, respectively. Toluene showed 26-fold lower toxicity than sulfuric acid. In this study, methanol and methylethylketone did not exhibit any toxicity to the earthworm. Further evaluation revealed that nitric acid, formic acid, and toluene exerted a change in the body weight of the chemically treated earthworms, whereas the other chemicals were ineffective. These results can be used for environmental risk assessment, when the chemicals are accidently discharged into the environment.
고수 정유를 원제로 하는 유제 제형의 잉어에 대한 급성독성
남태훈,전황주,김경남,최연서,이성은 한국환경생물학회 2016 환경생물 : 환경생물학회지 Vol.34 No.3
Essential oils (EOs) extracted from plants possess various biological activities and have been considered as natural insecticides due to their potent insecticidal activities. In regard to develop natural insecticides, EOs are formulated as an emulsifiable concentrate and their acute toxicity against to fishes were determined in a static condition using Cyprinus carpio. Coriander EO was used as an active ingredient mixed with ethanol for solvent and various surface active agents. The tested EOs were obtained from a commercial market, and three different extractions were also undertaken to produce EO using steam distillation, solvent extraction, and supercritical fluid extraction. Among the emulsifiable concentrate including a commercial coriander EO, surface active agents such as Tween 80, sodium dodecyl benzene sulfonate (SDBS), and mixture of SDBS and Nonidet showed acute toxicity to the fish. With the three different EO extraction, coriander EO obtained from supercritical fluids with Triton X-100 exhibited acute toxicity to C. carpio. Taken together, Tetgitol and Nondet are considered as surface active agents for the emulsifiable formulation of coriander EO.
김인건,남태훈,정익주,Kim, In-Geon,Nam, Tae-Hun,Chung, Ik-Joo 강원대학교 산업기술연구소 2013 産業技術硏究 Vol.33 No.1
The U-healthcare is increasingly important as there is a growing tendency for elderly people to acquire chronic disease such as heart problems and high blood pressure. In this paper, we implemented an oximeter which can interact and communicate with the smart devices such as smartphones through which users can manage their measuring data. The designed pulse oximeter is capable of detecting hemoglobin saturation levels in the range of 70-100% and the heart beat rate(BPM) in the range of 30-240 BPM.
Concentration and distribution of polychlorinated biphenyls in rice paddy soils
김이선,전진우,손지영,박민규,김철수,전황주,남태훈,김경남,박병준,최성득,이성은 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.2
To monitor and evaluate the risk of polychlorinated biphenyls (PCBs) contamination in Pohang, Korea, the concentration and distribution of 29 PCBs in paddy soils were determined using high-resolution gas chromatography/ high-resolution mass spectrometry. The overall concentrations of R29 PCBs in the paddy soils of the areas close to the heavily industrial city of Pohang (268–1833 pg g-1 dw) were higher than those in the paddies from Anseong (106.6–222.6 pg g-1 dw) in Korea. In Pohang, the major contributors to the R29 PCBs were the non-dioxin-like PCBs, including the PCBs 28, 52, 70, 101, 118, 138, 153, and 180, which correspond to 48–62% of the total PCBs. The toxic equivalency (TEQ) values obtained from the 12 dioxin-like PCBs from Pohang (0.03–1.03 pg TEQ g-1 dw) showed that PCB 126 contributed the highest toxicity, possibly posing a risk to the living organisms. The results of both principal component and cluster analysis based on the PCB homologue patterns demonstrated that each sampling site showed a similar PCBs contamination pattern, and Aroclor 1254, which is likely used by small and big steel factories, was identified as a major source of PCB contamination in Pohang.
04 포스터 발표 : 환경 화학 분야(PC) ; PC-10 : 농경지 토양 중 PCB의 분포
전황주 ( Hwang Ju Jeon ),구본화 ( Bon Hwa Ku ),남태훈 ( Tae Hun Nam ),김용찬 ( Yong Chan Kim ),이지형 ( Ji Hyoung Lee ),최재일 ( Jae Il Choi ),이성은 ( Sung Eun Lee ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-
Polychlorinated biphenyl (PCB) compounds are persistent and hazardous to the environment. They are frequently found in industrial areas because their uses were bound to industrial purposes. However, it has been reported that PCBs can be translocated to agricultural soils via various ways. Therefore, PCBs are also found in agricultural foods including crops. PBCBs are consisted of 209 congeners and analyzed by GC-ECD, GC-MS and HRGC-HRMS. In this study, 27 PCB congeners were used to monitor PCBs in agricultural soil samples collected in Chungcheong and Gyeonggi Provinces. Prior to analyze soil samples, method validations were undertaken to know precision, accuracy, and recovery using #209 PCB congener (DeCB1, decachlorobiphenyl) followed by a method certified Environment Protection Agency. Those validation results were acceptable and all procedures containing soxhlet extractions and column chromatography for clean-up were adopted for analysis. All soil samples were analyzed for their basic properties including soil organic contents and soil pH. All soil samples were undertaken by soxhlet extraction for 16 h. Silicagel column chromatography was employed to clean up the samples and the final eluate was evaporated and then redissolved in toluene after complete dryness. The samples were analyzed using GC-ECD. Some of PCB congeners were found in these analyses and finally needs to be confirmed by GC-Ms and HRGC-HRMS.