사람 난포액에 존재하는 Matrix Metalloproteinase-2 Isoform의 동정

나경아,김지수,심명선,권혁찬,이승재,윤용달,김해권 한국수정란이식학회 2002 한국수정란이식학회 학술대회 Vol.2002 No.1

포유동물의 암컷 생식기관에 존재하는 다양한 종류의 matrix metallo-proteinase (MMP)는 난소와 자궁의 구성성분의 주기적인 변화를 조절하며 이중 난소의 MMP는 난포의 성장과 배란 그리고 퇴화 동안 조직재구성에 매우 중요한 역할을 한다고 알려져 있다. 본 실험에서는 근래에 새로 발견된 사람의 난포액에 존재하는 분자량 약 110kDa인 MMP-2 isoform GA110을 동정하고 자 하였다. 난포액으로부터 GA110 단백질을 분리하기 위하여 난포액에 5mM ethylenediaminetetraacetic acid(EDTA)를 처리한 후 DEAE Sepharose Fast Flow를 이용한 chromatography를 시행하였다. 그 결과, 난포액 단백질들은 0.2M NaCl 의 분획에서 GA110 활성을 나타내었고 anti-human MMP-2 antibody에 대한 면역반응도 뚜렷이 나타났다. DEAE Sepharose Fast Flow에서 얻은 분획 중 GA110의 활성과 면역반응을 모두 나타내는 분획만을 모아 Gelatin Sepharose 4B affinity chromatography로 다시 분리하였다 분리한 결과 resin에 흡착된 단백질 (eluate) 분획들에서 매우 뚜렷한 GA110 gelatinase 활성을 나타내었으며 면역반응 또한 관찰되었다. 이 분획들의 단백질을 농축한 후 zymography를 시행하여 나타난 GA110 단백질 band를 잘라 내었으며 이로부터 단백질을 electroelution하여 농축한 후 reducing agent인 2-mercaptoethanol를 처리하였다. 이를 전기영동 후 MMP-2 (propeptide region) antibody를 사용하여 immunoblotting 한 결과 70-72kDa의 단백질만이 면역반응을 나타내었다. 마지막으로 위와 같이 준비된 70-72kDa 단백질의 아미노산 서열을 Edman degradation 방법으로 분석하였다. 그 결과 이 단백질의 N 말단의 10개의 아미노산 배열 순서가 알려진 사람의 proMMP-2의 전체 배열순서 중 propetide domain의 N 말단에서부터 다섯 번째에서 시작하여 10개의 아미노산의 서열과 정확하게 일치하였다. 위 결과들로 미루어 사람의 난포액에 존재하는 MMP-2의 새로운 isoform인 GA110은 70-72kDa의 ProMMP-2가 disulfide bond를 통해 homodimer 구조를 이루고 있는 것으로 여겨진다.

무용교육의 다학제 연구에 관한 고찰

나경아 한국예술종합학교 무용원 2020 무용과 이론 Vol.1 No.-

Dance is the artistic experience based on the physical activities through sensory awareness. Dance education valued as a practical studies that contributes for human development process. Dance education on the main contents of this paper is : first, to explain the value of dance education in the respect of integrating multiple fields of human abilities and creativity development. Second, to understand main contents of dance education research related with arts education, physical education and social education. This paper discussed functional and expressive aspects on the body and dance education in terms of personal development related with socialization. Finally, Through these study, this paper emphasizes that multidisciplinary studies of dance education can be applied in a various ways to education field.

김남천 전향소설의 탈식민성 연구

나경아 숙명여자대학교 한국어문화연구소 2008 한국어와 문화 Vol.4 No.-

The purpose of this study was to analyze all the conversion novels created by Kim, Nam-chun's since the China-Japan War from the postcolonial perspective and newly establish their position in Korean literature history. Therefore, this study observed social situations at the time where intellectuals under the Japanese colonial rule repeatied the logic of Japan's discourse to reproduce colonialism in society. He described converters who fell into self-contradictions as miserable and weak people who led self-reflection. In addition, Kim's conversion novel, found that he suggested the possibility of postcolonity though the use methods of 'sabotage and keeping distance'. In addition, the converters once again repeated Japan's logic to exercise a colonizer's power over other people in the group (people under Japanese rule). Female characters in Kim's conversion novels are placed in the double othering situation where they are suppressed by both Korean male intellectuals and new women who represent Japan or Pro-Japanese group. In addition, the female characters display the possibility of resistance in response to contradiction and flaws of colonial discourse.

세계민족무용 초청공연 리뷰

나경아 세계민족무용연구소 2002 민족무용 Vol.- No.1

국내 예술무용의 흐름과 동시대 창작의 주요 이슈

나경아 한국예술종합학교 무용원 2023 무용과 이론 Vol.7 No.-

Korean art dance has been created in various ways in the context of rapidly changing society and culture and the flow of modernist art dance. Contemporary dance, which has been developed through repeated innovations, has faced new issues related to creation in a changed environment. For a discussion related to this, first, we will examine the aspects of domestic artistic dance that have been developed by mixing the introduction of Western dance styles and the heritage of tradition in the historical flow. And I will discuss the changes in contemporary dance forms and issues related to the audience’s aesthetic experience.

무용형식의 무한한 가능성을 개척한 '포스트모던 댄스'

나경아 한국예술종합학교 2002 논문집 Vol.5 No.-

K-IFRS 도입 이후 손익보고양식에 따른 포괄손익과 당기손익의 가치관련성

나경아,강순경 한국국제회계학회 2015 국제회계연구 Vol.0 No.60

This study examines whether the presentation format of reported income affects the value relevance of comprehensive income and that of net income after the adoption of the Korean International Financial Reporting Standard (K-IFRS) in 2011, which allowed a firm to choose its presentation format of reported income between a single comprehensive income statement and two separate statements consisting of the income statement and the comprehensive income statement. To estimate the value relevance of comprehensive income and that of net income, we run the Ohlson(1995)’s price level model over the full sample of all listed firms during the first 3 years after the adoption of the K-IFRS(2011 - 2013). Major findings are as follows: The value relevance of comprehensive income and that of net income are higher when they are reported in separate statements compared to when they are reported in a single statement. These results imply that reporting the comprehensive income and the net income in separate statements can provide more useful information than those reported in a single statement. All the results continue to hold even if Heckman 2-stage least square procedure is used in order to control for endogeneity problem. Our findings can contribute to the current accounting literature by providing empirical evidence on the difference in the information usefulness of reported income based on different presentation format after the adoption of the K-IFRS. 본 연구는 한국채택국제회계기준(K-IFRS)도입 이후 손익보고 양식을 단일보고양식과 별도보고양식 중 선택할 수 있도록 자율성이 허용됨에 따라 손익의 보고양식 선택이 포괄손익과 당기손익의 가치관련성에 영향을 주는 지에 대하여 실증적으로 비교, 분석하였다. 이를 위하여 K-IFRS 도입 이후 3개 연도(2011연도 ~ 2013연도)동안 전체 상장기업을 대상으로 Ohlson(1995)의 주가수준 모형을 실시하여 포괄손익 및 당기손익의 손익보고양식에 따른 주가관련성의 차이를 검증하였다. 본 연구의 주요 분석결과는 다음과 같다. 포괄손익과 당기손익의 가치관련성은 하나의 포괄손익계산서에서 포괄손익과 당기손익이 함께 보고되는 것보다 별도의 손익계산서와 포괄손익계산서에서 각각 보고될 때 가치관련성이 더 높은 것으로 보인다. 이러한 결과는 당기손익은 손익계산서에서 그리고 포괄손익은 포괄손익계산서에서 따로 보고하는 별도보고양식을 선택하는 경우당기손익과 포괄손익이 더 뚜렷하게 구분되어 보고되므로 이 두 손익정보가 함께 한 보고서에보고되는 단일보고양식에 비해 자본시장의 정보이용자들의 이해력을 높일 수 있음을 시사한다. 추가분석에서 손익보고양식선택이 갖고 있는 내생성을 통제하기 위해 Heckman의 2단계모형을사용하여 분석을 한 경우에도 일관된 결과가 도출되었다. 본 연구결과는 K-IFRS도입 이후 손익보고양식 선택에 따른 손익정보 유용성의 차이에 대하여실증적 증거를 제공한다는 점에서 학계에 유익한 정보를 제공해 줄 것으로 기대된다.

다문화 가정 자녀들의 문화정체성 형성을 위한 무용교육 프로그램 개발 및 적용

나경아 한국무용기록학회 2012 한국무용기록학회지 Vol.24 No.-

Effect of solution viscosity on retardation of cell sedimentation in DLP 3D printing of gelatin methacrylate/silk fibroin bioink

나경아,신성철,이현지,신동혁,백지혜,곽호정,박민성,신종현,현진호 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.61 No.-

To improve the cell suspension of the gelatin-methacrylate(GelMA) bioink, the viscosity of the ink solution was controlled by biocompatible silk fibroin(SF) particles. The viscosity of the GelMA solution increased about 1.4 × 102 folds by the addition of 1 w/v% SF particles. The increase of solution viscosity reduced the displacement of cells and retarded the cell sedimentation significantly. SF-GelMA bioink containing 1 w/v% SF was non-cytotoxic and used for the 3D bioprinting by directly projecting a feature onto the bioink. The cells encapsulated in the structures showed a high level of metabolic activity and demonstrated that SF-GelMA was a biocompatible material.

운동학습의 관점에서 필라테스 학습에 관한 사례 연구

나경아 한국예술종합학교 무용원 2022 무용과 이론 Vol.5 No.-

Dance Wellness means practical education activity that actively promotes integrated development of humans. It includes not only healthy lives and improvement on performing ability of dancers but also meaning on wellness through dance. Overtraining with regard to dancer wellness leads to the deterioration of body function, thereby incurring injury or disturbing to express dance techniques. Pilates has been applied to as a training method for enhancing bodily functions and health care of dancers who have undergone problems due to excessive physical activity. Since visiting University of California, Irvine (UCI) in 2012, I have been carrying out research on Diane Diefenderfer’s Pilates Specialization for Dance Studies (PSDS) so far. In 2013, I introduced Diane’s PSDS at the 15th international conference organized by Korea National University of Arts, department of dance theory. After that, in both 2014 and 2018, I visited her studio (Studio du corps) on-site and conducted participant observation, interview, and data research. She has been succeeded classical Pilates of Ron Fletcher and Romana Kryzanowska and developed her own technique, with stretching for dancers included. The study was executed by dealing with emphasizing cognitive process in Pilates learning and aiming at enhancement on mind-body regulation capability by utilizing music. The class was held once a week for 14 weeks, comprising of one movement instructor and music therapist respectively, two participant observers, six students, and two video filmmakers. Those participants have recorded and analyzed their changing processes via observation, Journaling, video filming, interview, and so on. The main focuses on given class are first, promoting myoneural vitalization of core through prep works as well as analyzing one’s habitual posture and behavior pattern. Second, applying the most adequate Pilates music after watching myoneural function reacting to various music. Likewise, as a result of study, students recognized their postures and behavior patterns, and also the regulation capability on core muscles became strengthened, hence unnecessary physical tension turned out to be alleviated. In addition, sensory feedback with music dropped tension in muscles and heightened practice efficiency.