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중성자와 감마선 중복 조사가 자주달재비 분홍돌연변이율에 미치는 영향
김진규,이영근,김재성,신해식,현성희,Kim, Jin-Kyu,Lee, Young-Keun,Kim, Jae-Sung,Shin, Hae-Shick,Hyun, Soung-Hee 대한방사선방어학회 2000 방사선방어학회지 Vol.25 No.2
The effects of combined irradiation of neutrons and ${\gamma}$-rays were analysed by means of Tradescantia stamen hair (TSH) assay. Potted plants were irradiated with $0{\sim}2$ Gy of ${\gamma}$-rays. For the combined treatments, the plants were irradiated with neutrons from $^{252}Cf$ 24 hours before or after ${\gamma}$-irradiation. The slopes of dose-response curves were 5.98, 6.17 and 7.48, in ${\gamma}$-rays, ${\gamma}$-rays+neutrons, and neutrons+${\gamma}$ -rays irradiated groups, respectively. The biological efficacy of radiations in the induction of pink mutations increased by 25% in neutrons+${\gamma}$-rays irradiated group, while it increased by 12% in ${\gamma}$-rays+neutrons irradiated group. The combined irradiation with two kinds of radiations results in the different efficacy of radiation depending on the order of irradiations, which is of great importance in the related fields such as sterilization and radiotherapy. 자주달개비 식물체에 감마선과 중성자를 중복 조사하였을 패 나타나는 수술털(TSH) 분흥돌연변이율의 변화를 분석하였다. 감마선은 $0{\sim}2$ Gy의 범위에서 선량별 조사를 하였으며 중성자는 $^{252}Cf$ 선원으로부터 일정한 거리에 식물체를 배열하여 조사하였다. 감마선을 선량별로 조사한 후 중성자를 조사한 때의 TSH 분홍돌연변이 감마선량-반응의 증가계수는 6.17로 감마선량-반응식의 5.98에 비해 12%의 방사선효율 증가를 초래했으며 중성자를 먼저 조사한 다음 감마선을 조사한 경우는 증가계수가 7.48로서 25%의 방사선 생물효율 증대가 나타났다. 두 종류의 방사선 중복 조사시 조사방식의 선 후 조합에 따른 방사선생물효율 증대가능성은 멸균 또는 세포사멸 등 방사선을 긍정적으로 이용하는 분야에 있어 매우 중요한 의미가 있다.
김진규,김원록,이영근,김재성,송희섭,정철영,김기남,서원선,홍광표 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Jae Sung Kim,Hi Sup Song,Cheorl Young Jeong,Ki Nam Kim,One Sun Suh,Kwang Phyo Hong ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.
Artemia ( Class Crustacea ) 알의 부화에 미치는 이온화방사선의 효과
김진규,김원록,이영근,이창주,장화형 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Chang Joo Lee,Hwa Hyoung Chang ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.
자주달개비 수술털에서 방사선에 의해 유발되는 세포손상에 대한 살충제의 방어효과
김진규(Jin Kyu Kim),김원록(Won Rok Kim),이영근(Young Keun Lee),장화형(Hwa Hyoung Chang),이창주(Chang Joo Lee) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.1
To investigate the combined effect of radiation and pesticide on Tradescantia somatic cell mutations, potted plants of Tradescantia 4430 on which parathion had been sprayed evenly 24 hours before irradiation. Radiation doses were 0.3, 0.5, 1.0 and 2.0 ㏉ of gamma-ray. The plants irradiated only with the gamma-ray radiation were used as control groups(CT). Pink mutation frequency increased linearly proportional to the radiation dose and the peak interval of elevated mutation frequencies appeared during 7∼11 days after irradiation in both CT and Pa+γ groups. The slope of dose-response curve in CT was 5.99(r^2=0.988), while it was 3.43(r^2=0.981) in Pa+γ. It seemed that parathion pretreatment had a protective effect against radiation-induced cell damages since it decreased the slope value by 43%. It is suggested that an adaptive response or radiomodification could be induced in irradiated stamen hair cells by parathion pretreatment.
이영근(Young Keun Lee),장화형(Hwa Hyoung Chang),한갑진(Gab Jin Han),김진규(Jin Kyu Kim),김재성(Jae Sung Kim) 한국환경농학회 1998 한국환경농학회지 Vol.17 No.4
In order to determine the optimal radiation dose for the sterilization of biowastes, the bioburden, frequencies and radiosensitivities of bacteria, mold and fungi in rice straw, chaff, corn stover and sawdust was observed before and after γ-ray irradiation. Radiation sterilization dose of rice straw, chaff, com stover and sawdust was calculated as 17.7, 17.6, 15.6 and 20.0kGy, respectively, from the mutual compensation of screening dose and derived dose on the basis of 10^(-3) SAL. This method could be acceptable for the sterilization of various biowastes including food, pharmaceuticals, etc.
저선량 ${\gamma}$선 조사가 배추종자의 발아와 수량에 미치는 효과
김재성,김진규,이영근,백명화,김정규,Kim, Jae-Sung,Kim, Jin-Kyu,Lee, Young-Keun,Back, Myung-Wha,Gim, Jeong-Kyu 한국환경농학회 1998 한국환경농학회지 Vol.17 No.3
저선량 방사선에 의한 식물생육 촉진효과를 보고자 남방계 배추품종의 $1{\sim}5$년간 묵은 종자에 저선량 ${\gamma}$선을 $0.5{\sim}30.0Gy$까지 조사하여 온실과 포장에서 재배하여 그 생육상황을 조사한 결과는 다음과 같다. 배추 묵은 종자의 발아율과 유묘초장은 저선량 조사에 의해 향상되었으나 그 효과는 저장기간에 따라 달랐다. 저장기간이 5년차, 4년차, 3년차인 종자에게는 각각 4.0Gy, 1.0Gy, 0.5Gy 조사에서 발아율과 유묘초장 증가효과가 인정되었고 1년차와 2년차 종자에서 발아율 촉진효과는 없었으나 유묘초장은 증가하였다. 포장재배한 배추의 초장, 생체중, 직경으로 본 수량은 모든 저장종자의 4.0Gy와 8.0Gy 조사구에서 증가효과가 있었고 1년차와 2년차 종자에서는 20.0Gy와 30.0Gy에서도 수량증가 효과가 있었다. To observe the stimulating effects of low-dose ${\gamma}-radiation$ on the Chinese cabbage, $1{\sim}5$ years old seeds were cultivated in the green house and the experimental field after irradiation of $0.5Gy{\sim}30.0Gy$ and the growth characters of them were investigated. The germination rates and the seedling heights of old seeds increased positively in the green house, but different from each other depending on their period of storage. Those of 3-, 4-, and 5-year old seeds increased remarkably at the 4.0Gy-, 1.0Gy-, and 0.5Gy-irradiation group respectively. However, in the case of 1-year and 2-year old seeds, there were not significant effects of irradiation except the increase of seedling height. There were significant increasing effects of radiation on the plant height, fresh weight, and diameter of all seeds grown in the experimental field of 4.0Gy- and 8.0Gy-irradiation group, including the corresponding effects of 1-year and 2-year old seeds in 20.0Gy- and 30.0Gy-irradiation group.
방사선을 이용한 느타리 ( Pleurotus ostreatus ) 의 섬유소 분해 변이주 선발
이영근,장화형,김원록,이창주,김진규 ( Young Keun Lee,Hwa Hyoung Chang,Won Rok Kim,Chang Joo Lee,Jin Kyu Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
To isolate the cellulolytic variants, the basidiospores of Pleurotus ostreatus were irradiated at the dose of 1Gy∼20kGy of gamma-ray. After irradiation the germination and survival rates of basidiospores were observed and the activity of extracellular enzyme was determined by means of MUF-substrate method. The 45 variants showed various levels of mycelial growth rate and cellulolytic activity on solid media and vari ous levels of activities of the extracellular enzymes. We have isolated highly cellulolytic variants. 1.5KG-2 and 2KG-2. among the above variants. It is suggested that the isolated 2 variants could be applied usefully to reuse and/or recycle of the cellulosic bioresources.
쇠비름 수침액이 방사선 조사에 의한 생쥐의 생존율과 뇨중 아미노산 조성에 미치는 영향
천기정(Ki Jung Chun),김진규(Jin Kyn Kim),이영근(Young Keun Lee),김봉희(Bong Hee Kim) 대한약학회 1999 약학회지 Vol.43 No.2
A study has been conducted to investigate the radioprotective effects of purslane extracts (P) when I.p. injected before irradiation (R). For studying the amino acid composition, three groups of ICR mice (7-week-old) were tested. The first group I.p. injected with purslane extract for 5 days were irradiated with 6 Gy of gamma-radiation (P+R). The second group was irradiated with 6 Gy without any pretreatment (R). The third group was non-irradiated control (CT). Each group divided into two groups for the survival rate study, one injected with saline (S) and the other injected with purslane extract (P) for five days and then irradiated with 8 Gy. The amino acid composition of urine samples were analyzed with HPLC for 19 days after irradiation. A few kinds of amino acids such as lysine and methionine in urine from P+R group increased in comparison with those from CT or R group. The survival rate of P group maintained much higher than that of S group during experimental period. The results obtained may support that this plant has radioprotective substances by means of life-lengthening, not of urine amino acid components.