NaCl 처리와 감마선조사가 자주달개비 수술털 세포의 분홍돌연변이 유기에 미치는 영향

김진규(Jin Kyu Kim),김원록(Won Rok Kim),김재성(Jae Sung Kim),김기남(Ki Nam Kim),홍광표(Kwang Phyo Hong) 한국환경농학회 1999 한국환경농학회지 Vol.18 No.1

To investigate the combined effect of gamma ray irradiation and NaCl treatment on Tradescantia somatic cell pink mutations, potted plants of Tradescantia 4430 were evenly sprayed with NaCl solution(170mM) 24 hours before irradiation(NaCl+γ) and after irradiation(γ+NaCl). Irradiation doses were 0.3, 0.5, 1.0 and 2.0 Gy of gamma-ray. The plants irradiated only with gamma radiation were used as control group(CT). Frequency of pink mutation increased linearly with irradiation close and the peak interval of elevated mutation frequencies appeared during 6∼12 days aver irradiation in all the experimental groups. The slope of dose-response curve in CT was 5.99(r²=0.99), while it were 4.55(r²=0.98) in NaCl+γ and 4.33(r²=0.99) in γ+NaCl. It seemed that pre- and post-treatment of NaCl had a protective effect it against radiation-induced cell damages since it decreased the slope value by more than 24%. It is suggested that protective effect on DNA damages can be invoked in irradiated stamen hair cells by NaCl treatment.

Artemia ( Class Crustacea ) 알의 부화에 미치는 이온화방사선의 효과

김진규,김원록,이영근,이창주,장화형 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Chang Joo Lee,Hwa Hyoung Chang ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.

고리해역에서의 모악류의 계절변동

유광일,김원록 ( Kwang Il Yoo,Won Rok Kim ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.1

Seasonal variation of Chaetognaths in the coastal waters off Kori, was studied. Samples were collected monthly during the period from March 1988 to February 1989 at 30 stations. Ten species belonging to three genera were identified: Sagitta crassa, S. nagae, S. enflata, S. minima, S. regularis, S. robusta, S. neglecta, S. ferox, Pterosagitta draco and Krohnitta pacifica. Mean abundance of chaetognaths changed monthly from 0 to 165 individuals m^-3. The predominant species varied with month; Sagitta crassa occurred abundantly from April to July, S. enflata from August to November and S. nagae occurred throughout the year except for March.

남극반도 주변 Ice Edge Zone 에서의 하계 모악류의 분포

유광일,김원록,김동엽 ( Kwang Il Yoo,Won Rok Kim,Dong Yup Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.1

To study the distribution of chaetognaths in Ice Edge Zone near the Antarctic Peninsula during austral summer, samples were collected at 35 stations between Bransfield Strait and northwestern Weddell Sea from December, 1993 to January, 1994. Chaetognaths were consisted of three species, Enkrohnia hamata, Sagitta gazellae and Sagitta marri. The abundance of chaetognaths was 0∼1,359ind./1000㎥ and high values were observed in the northwestern Weddell Sea. Eukrohnia hamata and Sagitta gazellae were distributed widely in Bransfield Strait and northwestern Weddell Sea. The abundance of Eukrohnia hamata was high in northwestern Weddell Sea but it was dramatically dropped in the Bransfield Strait. The abundance of Sagitta gazellae was high in Gerlache Strait and northwestern Weddell Sea and S. marri was very low and showed restricted distribution. The vertical distribution of chaetognaths were mainly confined to 150m and highest density were found in bottom water.

하계 황해산 모악류의 분포

유광일,김원록 ( Kwang Il Yoo,Won Rok Kim ) 한국환경생물학회 1996 환경생물 : 환경생물학회지 Vol.14 No.2

Studies on the spatial distribution of chaetognaths have been carried out on the based of materials collected in the Yellow Sea in Auguest, 1982. As a result, 5 species belonging to two genera occurred; Sagitta crassa, S. enflata, S. nagae, S. regularis and Krohnitta pacifica. A key to the chaetognaths are given. Sagitta nagae was revealed to be a indicator species of the purturbed area formed by the mixing of the warm water current and neritic waters in summer season in the Yellow Sea.

방사선지표식물 자주달개비를 이용한 환경방사선 감시

김진규,김원록,이영근,김재성,송희섭,정철영,김기남,서원선,홍광표 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Jae Sung Kim,Hi Sup Song,Cheorl Young Jeong,Ki Nam Kim,One Sun Suh,Kwang Phyo Hong ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.

방사선을 이용한 느타리 ( Pleurotus ostreatus ) 의 섬유소 분해 변이주 선발

이영근,장화형,김원록,이창주,김진규 ( Young Keun Lee,Hwa Hyoung Chang,Won Rok Kim,Chang Joo Lee,Jin Kyu Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

To isolate the cellulolytic variants, the basidiospores of Pleurotus ostreatus were irradiated at the dose of 1Gy∼20kGy of gamma-ray. After irradiation the germination and survival rates of basidiospores were observed and the activity of extracellular enzyme was determined by means of MUF-substrate method. The 45 variants showed various levels of mycelial growth rate and cellulolytic activity on solid media and vari ous levels of activities of the extracellular enzymes. We have isolated highly cellulolytic variants. 1.5KG-2 and 2KG-2. among the above variants. It is suggested that the isolated 2 variants could be applied usefully to reuse and/or recycle of the cellulosic bioresources.

사탕수수 찌꺼기를 이용한 여름느타리 발효부산물의 생물활성

이영근,장화영,김원록,김진규,김재성 ( Young Keun Lee,Hwa Hyoung Chang,Won Rok Kim,Jin Kyu Kim,Jae Sung Kim ) 한국환경농학회 1998 한국환경농학회지 Vol.17 No.1

In order to evaluate the biological activities of some fractions from the fungal(Pleurotus sajorcaju) fermentation products of sugar cane bagasses, the antimutagenicity, the glyceollin elicitor activity on soybean and the effect on the stem elongation in pea were observed. The alcohol extract fraction and DMSO soluble fraction had excellent antimutagenicity even though it is weaker than that of the extracts from the fruiting bodies. All of the extracts had the ability to elicit glyceollins in soybean cotyledons and these extracts could be helpful for plants to protect themselves from pathogenic contaminations. IAA and the extracts had shown synergistic effects on pea stem elongation in all experimental groups positively detemlined.

자주달개비 수술털에서 방사선에 의해 유발되는 분홍돌연변이에 대한 광주기의 영향

김진규(Jin Kyu Kim),김원록(Won Rok Kim),(A . Cebulska Wasilewska) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.3

The present study was carried out to investigate the combined effect of radiation and photoperiod (PP) regimes on Tradescantia 4430 somatic cell mutations. Potted plants were irradiated with 0.3, 0.5 and 1.0 Gy of gamma radiation from ^60Co source. The plants irradiated only with gamma radiation were used as control group (CT). The somatic cell mutation rate in 0.5 Gy irradiated CT and PP20 group started to increase on the 6th day and reached a maximum value on the 10th day and 9th day after irradiation while the rate in the experimental group under 4 hours of photoperiod a day (PP4) started to increase on the 10th day and reached a maximal value on the 16th day post-irradiation. The slope of dose-response curve in CT was 5.99 (r^2=0.99), while it was 6.93 (r^2=0.98) in PP20 and 11.74 (r^2=0.99) in PP4, respectively. The biological efficacy of radiation in the induction of pink mutation increased by 15.7% in PP20 and 95.9% in PP4, respectively. It is suggested that photoperiod regimes unfavorable to the plant have an additive effect on radiation-induced mutations and a delaying or inhibiting effect on cell damage repair, as well. [photoperiod, pink mutation, radiation, Tradescantia].

기온일교차와 감마선의 영향에 의한 자주달개비 수술털의 체세포 돌연변이 빈도

김진규(Jin Kyu Kim),김원록(Won Rok Kim),김재성(Jae Sung Kim),신해식(Hae Shick Shin),이정주(Jeong Joo Lee) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.3

This study deals with the effects of diurnal temperature difference (DTD) on somatic cell mutation frequencies in Tradescantia stamen hairs irradiated with radiation. Potted plants of Tradescantia 4430 were irradiated with 0.3, 0.5, 1.0 and 2.0 Gy of gamma radiation. The irradiated plants were maintained under two different experimental conditions; at constant temperature of 20℃ (DTD0) and at 28℃ for 14-h day and 8℃ for 10-h night (DTD20). The somatic cell mutation rate in 0.5 Gy irradiated group showed a big increase on the 6th day and reached a maximum value on the 10th day after irradiation while the rate in the experimental group under the condition of DTD20 started to increase on the 8th day and got to a maximal value on the 14th day postirradiation. In both of the two experiments, the dose-response relationships were clearly linear. The slope of the DTD20 dose-response curve was much steeper than that of the DTD0 one. In conclusion, a great DTD, as one of environmental stresses, enhanced the effectiveness of radiation in the induction of somatic cell mutations and caused a shift of the peak interval of radiation-induced mutations in Tradescantia stamen hairs.