중성자 조사후 Black mouse의 고환 조직 및 정자수 변화에 관한 연구

천기정,서원숙,손화영,Chun, Ki-Jung,Seo, Won-Sook,Son, Hwa-Young 대한방사선방어학회 2006 방사선방어학회지 Vol.31 No.1

Black mouse에 하나로 원자로의 BNCT시설을 이용하여 중성자(flux:1.036739E+09)를 머리를 정면으로 16 및 32 Gy 조사한 후 생물학적 효과를 관찰하는 일환으로 고환에 대한 물리학적 변화 및 조직 변화를 관찰하였다. 조사 후 90일이 경과한 후에 고환의 무게는 변화가 없었으나 고환의 부피는 약간 감소하였으며, 정자수도 감소하였다. 고환의 조직검사에서는 32 Gy 중성자 조사군에서 위축된 정세관의 수가 증가되었으며 stage VI에서의 정세관에서는 정조세포 및 비사기 정모세포가 고갈되어 있음을 알 수 있었다. 중성자 조사(32 Gy)후 고환의 손상이 장기간 경과 후에도 회복되지 않음을 알 수 있었다. For the purpose of the biological effect in black mouse by neutron irradiation, mice were irradiated with 16 or 32 Gy neutron (flux: 1.036739E+09) by tying flat pose at BNCT facility on HANARO Reactor. And 90 days later of irradiation, physical changes of testis and testis tissue were examined. There were no weight changes but a little bit volume changes and sperm counts in the testes. Atrophy of seminiferous tubules irradiated with 32 Gy neutron is increased in number and severity and those in stage VI showed depletion of spermatogonia and pachytene spermatocytes compared to the non-irradiated control group. Testis damage of black mouse was not recovered after long time by 32 Gy neutron irradiation.

BSH 존재시 중성자 및 ${\gamma}$-ray 조사에 따른 plasmid DNA의 손상

천기정,서원숙,Chun, Ki-Jung,Seo, Won-Sook 대한방사선방어학회 2006 방사선방어학회지 Vol.31 No.2

In this study, the extent of plasmid DNA damage was observed according to concentration of BSH(Boron Sulfhydryl Hydride) and irradiation doses of neutron and ${\gamma}$-ray. The plasmid used was both pBR 322 (2870 bp) and ${\Phi}X174$ RF(5386 bp) DNA. Plasmid DNA damage by irradiation in the presence of BSH was analyzed by agarose gel electrophoresis. In the neutron experiment, DNA damage of both plasmid DNAs was increased according to increasing the concentration of BSH and neutron doses. But in the ${\gamma}$-ray experiment, there appeared no dose dependency as compared to the neutron experiment. The extent of the plasmid DNA damage in the presence of BSH was somewhat different according to irradiation by neutron or ${\gamma}$-ray. 본 연구에서는 boron 화합물인 BSH(Boron Sulfhydryl Hydride)의 농도와 중성자 및 감마선 방사선 조사선량에 따른 plasmid DNA의 손상 정도를 관찰하였다. Plasmid는 pBR 322(2870 bp)와 ${\Phi}X174$ RF DNA(5386 bp)를 사용하였고 조사 후 DNA의 손상 정도는 agarose gel 전기영동 상에서 관찰하였다. 중성자 조사에서는 plasmid DNA의 손상 정도는 BSH의 농도 및 조사 선량이 증가함에 따라 증가하였으나 감마선 조사에서는 조사하지 않은 대조군과 큰 차이를 나타내지 않았다. Plasmid DNA의 손상 양상은 BSH 존재시 중성자 및 감마선 조사에서 다소 다름을 알 수 있었다.

생쥐 공장 융모의 감마선 전신조사에 의한 형태학적 변화

천기정,김진규,송치원,김무강,Chun, Ki-Jung,Kim, Jin-Kyu,Song, Chi-Won,Kim, Moo-Kang 대한방사선방어학회 2000 방사선방어학회지 Vol.25 No.4

소화기계 암의 효과적인 치료중의 하나로 방사선 치료법을 들 수 있으나 이 방법은 장 점막과 같이 빠르게 증식하는 조직에는 특히 부작용을 초래할 수 있다. 생쥐의 공장 융모가 감마선 전신 조사에 의해 형태학적으로 변화하는 정도를 관찰하였다. 생후 4주된 ICR계 생쥐를 6.5Gy와 12Gy를 전신 조사 3일 후에 공장을 채취하여 탈수 과정을 거쳐 파라핀을 입힌 조직을 절편기로 잘라 슬라이드에 부착시키고 일반적인 hematoxylin & Eosin 염색과정을 거쳐 염색후 광학현미경으로 관찰한 결과, 6.5Gy와 12Gy를 조사 받은 생쥐의 융모는 대체로 정상생쥐와 비교 할 때 융모의 길이가 짧아졌으며 융모의 두께가 비후되었고 내강은 넓어지는 경향을 나타내었다. 따라서 융모가 소화과정에 중요한 역할을 담당하며 방사선에 비교적 민감함을 나타내므로 방사선에 의한 생체영향 평가 뿐만아니라 방사선 방어제를 검색하는데 하나의 실험 파라메타가 충분히 될 수 있음을 인지하였다. Radiotheraphy may be considered as one of the most effective treatments for digestive tumors. This procedure has major side effects, especially in fast growing tissues like intestinal mucosa. For this reason, the morphological changes in mouse jejunal villi after whole body ${\gamma}-irradiation$ were analysed. Four week old ICR male mice were irradiatied with 6.5Gy or 12Gy of whole body ${\gamma}-irradiation$ and were sacrificed 3 days later. Jejunum among intestine were taken for morphology. Samples were carried out dehydration process and sliced sample covered with paraffin was hold on the slide and then light microscopy was observed by Hematoxylin & Eosin staining. villi at both irradiated doses were showed that the length of villi were shortened and thickened and that lumen were expanded in comparison with non-irradiated group. Since willi have an important role in digestion and very sensitive to radiation from this test, it has a role of test parameter for finding radioprotectors as well as evaluating the biological effect by radiaton.

흰쥐 조직에서의 카드뮴 결합 고분자량 단백질의 유도

천기정(Ki Jung Chun),김봉희(Bong Hee Kim) 대한약학회 1997 약학회지 Vol.41 No.3

The study was carried out on the biochemical characters of Cd-BP(I) after isolation and purification of the protein from the liver of rat injected intraperitoneally with Cd. A continued study has been doing whether Cd-BP(I) could be induced by Cd or by other metals such as Zn and Cu. Antisera were made against the Cd-BP(I) from NewZealand white rabbits. Carried out were gamma-globulin purification, then Ouchterlony test adn gel immunodiffusion test. Cd-BP(I) was also found in normal tissues of rat. It was induced up to a considerable level by Cd, whose induced level was higher than that of Cu or Zn treatment. The level of induction by Cu or Zn pretreatment plus Cd treatment was lower than that by simple treatment of Cu or Zn. Such a result was presumably related to the Cd toxicity.

감마선에 조사된 생쥐에 있어서 방사선방어효과 평가를 위한 생물학적 파라메타

천기정(Ki Jung Chun),김봉희(Bong Hee Kim),이영근(Young Keun Lee),김진규(Jin Kyn Kim) 대한약학회 1999 약학회지 Vol.43 No.2

This study deals with the biological changes in mice after gama-irradiation. Four weeks old BALB/c mice were irradiated with 6.5Gy of gamma-ray on the fifth day after oral administration of radioprotectants such as ascorbic acid, tocopherol and cysteine. Control group was irradiated with 6.5Gy without pre-administration of radioprotectors. Blood cells and sperm cells were counted and body, testis and spleen were weighed 3 days after irradiation. And also liver antioxidant activity and range of spleen immune cells were measured. Differences in most biological parameters were not clearly distinguished between experimental groups. However, the relative spleen weight, the relative testis weight and the population size of spleen immune cells such as T helper cells, B cells and macrophages measured by means of FACS showed significant difference between irradiated and radioprotectant administered group. It is concluded that the relative spleen weight, the relative testis weight and the population size of spleen immune cells are easy and useful parameters for assessing the effect of radioprotective substances and for quantifying biological damage of radiation, as well.

쇠비름 수침액이 방사선 조사에 의한 생쥐의 생존율과 뇨중 아미노산 조성에 미치는 영향

천기정(Ki Jung Chun),김진규(Jin Kyn Kim),이영근(Young Keun Lee),김봉희(Bong Hee Kim) 대한약학회 1999 약학회지 Vol.43 No.2

A study has been conducted to investigate the radioprotective effects of purslane extracts (P) when I.p. injected before irradiation (R). For studying the amino acid composition, three groups of ICR mice (7-week-old) were tested. The first group I.p. injected with purslane extract for 5 days were irradiated with 6 Gy of gamma-radiation (P+R). The second group was irradiated with 6 Gy without any pretreatment (R). The third group was non-irradiated control (CT). Each group divided into two groups for the survival rate study, one injected with saline (S) and the other injected with purslane extract (P) for five days and then irradiated with 8 Gy. The amino acid composition of urine samples were analyzed with HPLC for 19 days after irradiation. A few kinds of amino acids such as lysine and methionine in urine from P+R group increased in comparison with those from CT or R group. The survival rate of P group maintained much higher than that of S group during experimental period. The results obtained may support that this plant has radioprotective substances by means of life-lengthening, not of urine amino acid components.

흰쥐 간에서의 카드뮴 결합 고분자량 단백질의 분리 및 정제(I)

천기정(Ki Jung Chun),김봉희(Bong Hee Kim) 대한약학회 1994 약학회지 Vol.38 No.5

We isolated two Cd-binding high molecular weight proteins from intraperitoneally cadmium injected rat liver. Molecular weight of Cd-BP(I) purified from Sephacryl S-100 and DEAE Sepharose column chromatography and Cd-BP(II) purified from DEAE Sepharose column chromatography and Sulphonyl Sepharose column chromatography was 33,000 and 18,400, respectively. Alcohol dehydrogenase and alkaline phosphatase acitivities were not detected from two purified proteins.

흰쥐 조직에서 카드뮴 결합 고분자량 단백질 [Cd-BP(II)]의 유도

천기정(Ki Jung Chun),김봉희(Bong Hee Kim) 대한약학회 1999 약학회지 Vol.43 No.5

The study was carried out on the biochemical chracters of Cd-BP(II) after isolation and purification of the protein from the liver of rat ip injection with Cd. A continued study has been doing whether Cd-BP(II) could be induced by Cd or by the other metals such as Zn and Cu. Antisera were made against the antigen of Cd-BP(II) from New Zealand white rabbits. We carried out g-globulin purification, then Ouchterlony test and gel immunodiffusion test. Cd-BP(II) was also found in normal tissues of rat. It was induced up to a considerable level by Cd, whose induced level was higher than that Cu or Zn treatment. The level of induction by Cu or Zn pretreatment plus Cd treatment was lower than that by single treatment of Cu or Zn. Such a result was presumably related to the Cd toxicity.

카드뮴 결합 고분자량 단백질의 생화학적 특성

천기정(Ki Jung Chun),김봉희(Bong Hee Kim) 대한약학회 1995 약학회지 Vol.39 No.4

The isoelectric points of Cd-BP(I) and Cd-BP(II), cadmium-binding proteins, were 6.01 and 5.35, respectively. Both of them contained zinc. As for the amino acid composition, Cd-BP(I) contained a lot of glycine and lysine but none of such aromatic amino acids as tyrosine and phenylalanine. On the other hand. Cd-BP(II) contained leucine, histidine, aspartic acid and alanine but no aromatic amino acids.

하나로 원자로 BNCT 열중성자 조사장치에 대한 선량특성연구

이동한,서소희,지영훈,최문식,박재홍,김금배,류성렬,김명섭,이병철,천기정,조재원,김미숙,Lee, Dong-Han,Suh, So-Heigh,Ji, Young-Hoon,Choi, Moon-Sik,Park, Jae-Hong,Kim, Kum-Bae,Yoo, Seung-Yul,Kim, Myong-Seop,Lee, Byung-Chul,Chun, Ki-Jung,Cho, 한국의학물리학회 2007 의학물리 Vol.18 No.2

최대출력 30 MW, 하나로(HANARO) 다목적 연구용 원자로의 접선 중성자공에 붕소중성자포획치료(Boron Neutron Capture Therapy, BNCT)를 위한 열중성자 조사장치가 개발되었다. BNCT 조사장치에서는 서로 다른 물리적 특성과 생물학적 효과비를 가진 여러 성분의 방사선이 방출되기 때문에 정확한 투여선량을 결정하기 위해서는 각 성분의 정량적 분석이 필수적이다. 따라서 본 연구에서는 방사화 분석, 열형광선량계 및 이온전리함 등 여러 유형의 검출기를 사용하여 BNCT 조사장치에서 방출되는 열중성자 및 감마선 혼합장의 선량 성분을 분리, 측정하였다. 선량측정은 물 속에 함유된 불순물과 중성자의 이차반응을 최소화하기 위해 증류수를 채운 물팬텀을 이용하였다. 그리고 측정 결과는 MCNP4B 전산계산의 결과와 상호 비교하였다. 측정 결과 열중성자속은 물팬텀 10 mm와 20 mm 깊이에서 각각 $1.02E9n/cm^2{\cdot}s$과 $6.07E8n/cm^2{\cdot}s$이었고, 고속중성자선량율은 10 mm 깊이에서 0.11 Gy/hr로 미세하였다. 감마선량률은 물팬텀 20 mm 깊이에서 5.10 Gy/hr로 나타났다. 측정된 중성자와 감마선량값은 MCNP의 결과와 5% 이내로 잘 일치하였고, 열중성자속은 14%의 비교오차를 나타내었다. 이러한 결과들은 중성자 검출의 난이도를 고려할 때 충분히 신뢰할 수 있는 수준이라 판단되며, BNCT 임상 연구를 위한 선량평가 자료로 활용할 수 있을 것으로 사료된다. A thermal neutron beam facility utilizing a typical tangential beam port for Neutron Capture Therapy was installed at the HANARO, 30 MW multi-purpose research reactor. Mixed beams with different physical characteristics and relative biological effectiveness would be emitted from the BNCT irradiation facility, so a quantitative analysis of each component of the mixed beams should be performed to determine the accurate delivered dose. Thus, various techniques were applied including the use of activation foils, TLDs and ionization chambers. All the dose measurements were perform ed with the water phantom filled with distilled water. The results of the measurement were compared with MCNP4B calculation. The thermal neutron fluxes were $1.02E9n/cm^2{\cdot}s\;and\;6.07E8n/cm^2{\cdot}s$ at 10 and 20 mm depth respectively, and the fast neutron dose rate was insignificant as 0.11 Gy/hr at 10 mm depth in water The gamma-ray dose rate was 5.10 Gy/hr at 20 mm depth in water Good agreement within 5%, has been obtained between the measured dose and the calculated dose using MCNP for neutron and gamma component and discrepancy with 14% for fast neutron flux Considering the difficulty of neutron detection, the current study support the reliability of these results and confirmed the suitability of the thermal neutron beam as a dosimetric data for BNCT clinical trials.