사람의 골수 줄기 세포로부터의 골세포 분화 과정에서 BMP-2가 미치는 영향과 그에 따른 분화 유전자의 발현 비교 연구

김인숙,장옥련,조태형,이규백,박용두,노인섭,황순정,김명진,이종호,Kim, In-Sook,Zhang, Yu-Lian,Cho, Tae-Hyung,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Hwang, Soon-Jung,Kim, Myung-Jin,Lee, Jong-Ho 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1

It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.

쥐의 골수로부터 추출한 줄기세포를 이용한 조골세포로의 분화 유도과정에서 나타난 문제점에 관한 분석 연구

김인숙,조태형,장옥련,이규백,박용두,노인섭,이종호,김명진,황순정,Kim, In-Sook,Cho, Tae-Hyung,Zhang, Yu-Lian,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Lee, Jong-Ho,Kim, Myung-Jin,Hwang, Soon-Jung 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1

This study was aimed to characterize osteogenic potential of rat bone marrow stromal cells (BMSC) isolated with standard flushing method and investigate the plasticity of transdifferentiation between osteoblastic and adipocytic lineage of cultured BMSC. Unlike aspiration method in human, rat bone marrow was extracted by means of irrigation with culture media that elevates the possibility of co-extraction of committed osteoprogenitor, or preosteoblast or other progenitor cells of several types present inside bone marrow. The cultured stromal cells showed high ALP activity which is representative marker of osteoblast without any treatment. Osteogenic inducers such as Dex and BMP-2 were examined for the evaluation of their effect on osteogenic and adipocytic differentiation of stromal cells, because they function as osteoinductive agent in stromal cells, but simultaneously induce adipogenic differentiation. Osteogenic differentiation was evaluated by measuring alkaline phosphatase activity or mRNA expression of osteoblast markers such as osteopontin, bone sialoprotein, collagen type I and CbfaI, and in vitro matrix mineralization by von Kossa staining. Oil red staining method was used to detect adipocyte and adipocytic marker, aP2 and $PPAR{\gamma}2$ expression was examined using RT-PCR. It can be supposed that irrigation procedure resulted in high portion of already differentiation-committed osteoprogenitor cell showing elevated ALP activity and strong mineralization only under the supplement of $100{\mu}M$ ascorbic 2-phosphate and 10mM ${\beta}$-glycerophosphate without any treatment of osteogenic inducers such as Dex and BMP-2. Dex and BMP-2 seemed to transdifferentiate osteoprogenitor cells having high ALP activity into adipocytes temporarily, but continuous treatment redifferentiated into osteoblast and developed in vitro matrix mineralization. This property must be considered either in tissue engineering for bone regeneration, or in research of characterization of osteogenic differentiation, with rat BMSC isolated by the standard irrigation method.

지역 청년창업생태계 조성을 위한 대학의 지원방안 탐색: 서울시 사례를 중심으로

김인숙,양지희,Kim, In Sook,Yang, Ji Hee 한국벤처창업학회 2022 벤처창업연구 Vol.17 No.4

The purpose of this study was to analyze the perception and demand of local youth and to find ways to support universities in order to create an youth start-up ecosystem. To this end, 509 young people living in Seoul were analyzed to recognize and demand young people in the region for youth start-ups, and to support universities. The findings are as follows. First, as a result of analyzing young people's perception of youth start-ups in the region, the "Youth Start-up Program" was analyzed the highest in terms of the demand for regional programs by university. In addition, there was a high perception that the image of youth startups in the region was "challenging" and "good for changing times." Second, after analyzing the demand for support for youth start-ups in the region, it appeared in the order of mentoring, start-up education, and creation of start-up spaces. And it showed different needs for different ages. Third, the results were derived from analysis of the demand for university support for the creation of a regional youth start-up ecosystem, the criteria for selecting local youth start-up support organizations, and the period of participation in local youth start-up support. Based on the results of the above research, the implications and suggestions of university support for the creation of a community of youth start-up ecosystem are as follows. First of all, it is necessary to develop and operate sustainable symbiosis mentoring programs focusing on university's infrastructure and regional symbiosis. Second, it is necessary to develop and utilize step-by-step systematic microlearning content based on the needs analysis of prospective youth start-ups. Third, it is necessary to form an open youth start-up base space for local residents in universities and link it with the start-up process inside and outside universities. The results of this study are expected to be used as basic data for establishing policies for supporting youth start-ups and establishing and operating strategies for supporting youth start-ups at universities.

VMD전략을 위한 VMD, 공간구조, 행태간의 상호관계성 연구 - 패션 리테일 매장을 중심으로 -

김인숙(Kim, In-Sook),김영욱(Kim, Young-Ook) 대한건축학회 2020 대한건축학회논문집 Vol.36 No.12

VMD is a marketing strategy at the final stage that adjusts the level of visual product planning so that all corporate activities are visible in a physical space, and it requires quantitative and objective establishment. Unlike other channels of marketing communication, however, there is no objective criterion for controlling visual product planning when deciding in-store merchandising according to VMD. In order to establish a VMD strategy, it is important to fundamentally clarify the interrelationships of the properties of products and space, and responses of customers. Therefore, the purpose of this study is to determine the level of VMD strategy by verifying the efficiency of merchandise presentation(MP), an implementation method of image-centered and product-centered VMD through the interrelationship between spatial configuration and behavior. This study is consists of three subdivisions. First, the VMD strategy of the target fashion retail store is analyzed by dividing it into VP(visual presentation), PP(point of sale presentation), and IP(Item Presentation) based on MP. Second, the spatial configuration of the target fashion retail stores is analyzed based on MP. Third, the usage behavior of the target fashion retail space is analyzed based on MP. As these results, the correlation between VMD, spatial configuration, and usage behavior is analyzed. Only significant results based on correlation analysis and regression in identical products and periods in two fashion retail stores are analyzed, and space for recognized images(VP) and sales space(PP, IP) are compared for objectively and quantitatively. As a conclusion, the image recognition space(VP) is more strategically placed than the sales space(PP, IP). The sales space, however, is more attractive to the customers due to the impact on accessibility and visibility in actual utilization. This quantitative analysis can be the basis for the spatial planning of the VMD strategy of fashion retail stores. It will enable a retail shopping environment in line with changes in the market by presenting the basis for the efficiency of the VMD strategy in fashion retail.

전북지역 주부들의 외식행동과 음식물 쓰레기에 대한 의식 조사

김인숙,유현희,박선화,Kim, In-Sook,Yu, Hyeon-Hee,Park, Sun-Hwa 한국식생활문화학회 2000 韓國食生活文化學會誌 Vol.15 No.5

The purpose of this study was to investigate several aspects of eating out behavior especially in relation to food waste. The data were collected from 620 housewives in Jeonbuk area but, except for 134 case not eating out . The 66.9% of subjects ate eating out 1 to 2 times monthly. The younger in age, higher in education level and employed housewives, higher in monthly income have a more increase in eating out frequency. In the 50.8% of subjects food rest was rare when eating out. The higher in educational level and higher in monthly income have an inclination to more food waste. The 51.4% of subjects don't know [Good Menu System]. We housewives should order a planned and moderate food amount when eating out, and make an effort normally anywhere for the reduction of food waste, and then the wasted food and food rubbish will be reduced. Besides this, the restaurants should practice [Food Bank] & [Good Menu System] and the academic world should make further researches on this field. Moreover the government administration should enlighten the people and have a public information for the more reasonable food culture.

상부 위장관의 (胃腸管) 위내시경 (胃內視鏡) 검사 2470에 대한 고찰

김인숙,이원경,이충식,이은기,정을순,최용직 ( In Sook Kim,Won Kyung Lee,Choong Sik Lee,Eun Ki Lee,Eul Soon Chung,Yong Gik Choi ) 대한소화기학회 1981 대한소화기학회지 Vol.13 No.2

The endoscopic findings were compared with the radiologic findings and the following results were obtained. 1) In the gastrofiberscopic findings, the main stomach diseases were gastritis, benign ulcer, stomach cancer in order of frequency, and duodenal ulcer, esophageal diseases k bezoars were also noted. In gastritis, chronic superficial gastritis was the most common with 47. 9%. 2) In normal group on gastrofiberscopy, the sex ratio was nearly same and the most fre- quent age group was 6th decade with the incidence of 28. 5%. In gastritis, male to female ratio also was same, and the most common age group was 5 th decade, In gastric ulcer, the sex ratio was 1. G:1 with male preponderance and in age distribution, 5 th was common, while stomach cancer heing 1.3:1 R 6 th decade was the most frequent occurrence. 3) In the advanced stomach cancer, endoscopic gross finding by Bormann's classification was as follows; type g (32.9%), type p (23.1po), type ]I (18.1%), type I (14. 0%). So called early cancer was 4. 5%. 4) In gastric ulcer, the site of lesion revealed that the antrum was the highest frequency (43. 3%), the next was angle (33. 3%), and the body (23. 7%) in order, while in gastric cancer, a e antrum was 40. 7%, the body was 32. 1% and the angle was 27. 2%, 5) In relationship between radiologic and endoscopic findings equality was noted in 79, 3% of gastritis, in 89. 5% of gastric ulcer and in 98. 7%of gastric cancer. 6) Of the total 2470 cases, 1303 cases were associated with other diseases, as follows; liver diseases(24. 7%), anemia(16. 2%), helminthiasis(14%), neurosis(6. S%), pulmonary tuberculosis (6. 7%), 6-U tractinfection (8. 2%), and so on.

조선조 이상향(理想鄕)의 작품분석 연구

김인숙(Kim, In Sook) 한국전시산업융합연구원 2014 한국과학예술융합학회 Vol.17 No.-

동양의 이상향은 자연을 제재로 하여 전통 문학과 산수화에서 구체적으로 묘사되어 왔다. 특히 조선조는 유교의 이상 국가 건설을 의도적으로 지향한 이데올로기적 국가였으므로 이상향에 관한 문화적 구성으로서 시와 산수화가 지속적으로 생산됐다. 본고는 이와 같은 조선조의 이상향 전개를 작품분석을 통하여 조망하고자 한다. 우선 이상향을 구성하는 담론내용을 살피면 국초의 강력한 유교국가 지향에도 불구하고 당대의 이상향에는 고려조의 유 · 불 · 선이 절충된 개념들이 혼재되어 있었다. 본격적인 유교적 이상향은 중기 이후, 정치적 주체로 등장하는 사림의 산림적 이상향으로부터 이들이 경화사족화함에 따라 나타나는 성시산림적 이상향으로 나누어 전개됐으며 또한 조선조 후기 이후에는 신분제 해체에 의한 중인층의 부상에 따라 그 동안 억압되었던 현세구복적 이상향과 도가적 이상향이 나타난다. 본고에서는 이와 같은 조선조 이상향의 변화에 관하여 각각의 이상향을 표상한 작품 <몽유도원도>,<곡운구곡도>, <청풍계도>, <민화 소상팔경도>, <도원문진도>를 통하여 분석한다. 이와 같이 산수화의 형식으로 표상된 조선조 이상향의 장소성은 당대를 살았던 주체들이 그들이 살았던 사회에 대한 인식과 소망 등에서 비롯된 자연과 문화의 복합적 구성물로서 장소감을 넘어 장소정신으로 확장되면서 조선조의 이상향을 변화시켰다. The Asian utopia has been specifically described in traditional literature and landscape painting with a subject of nature. Especially since Cho-Sun dynasty was an ideological country intentionally directing to confucian country establishment, for the whole Cho-Sun dynasty period, poems and landscape paintings had been continuously produced as a literary composition regarding to utopia. This paper tries to take a view of utopia expansion of Cho-Sun dynasty through landscape painting work analysis. Firstly, when observing the discussion contents which consist utopia, there are notions compromised of Korea dynasty"s Yoo, Bul, Sun mingled in utopia of the time in spite of strong inclination to the confucian nation of early country establishment. The main confucian utopia had been expanded as being divided into Sung-Si-San-Lim utopia which appears according to Kyung-Hwa-Sa-Jok-Hwa from Sa-Lim"s San-Lim utopia appearing as a political subject after the middle period. Also after late Cho-Sun dynasty there appears double utopias, the upper class shows the taoistic utopia and the lower class shows basic utopia which had been oppressed for a while due to the rise of the people by hierarchial dismantlement. This paper analyses these kinds of changes of Cho-Sun dynasty utopia through works of 〈Mongyudowondo〉, 〈Gokungugokdo〉,〈Chungpungke〉, 〈Minhwa Sosangpalgyungdo〉, 〈Dowonmunjindo〉 which expressed each of the utopia. Sense of place of Cho-Sun dynasty utopia which expressed into a form of the landscape painting changed the Cho-Sun dynasty"s utopia as the main people living at the time expanded it to the place spirit beyond feelings of place as a composition of nature and culture which originated from the hope and perception about the society they were living in.

여성노인의 차(茶)문화치료 융합프로그램 경험 현상 연구: 주간보호센터 이용 노인대상으로

김인숙,박선희,Kim, In-Sook,Park, Sun-Hee 한국사물인터넷학회 2021 한국사물인터넷학회 논문지 Vol.7 No.1

The purpose of this study is to explore elderly women's experience of the convergent tea culture therapy program when they use day care centers, ultimately getting a better understanding of such experience. For the purpose, the study made profound interviews with 4 old women who experienced the convergent tea culture therapy program from its start to final session. That program was provided by H day care center located in P city. And then, this research analyzed data from those interviews by using the phenomenological methods. As a result of the analysis, the study could divided the surveyed female elders' experience of the above mentioned program largely into 4 categories, which are, 'getting away from daily routines', 'feeling as if I'm the queen when having tea', 'finding myself feeling younger with tea', and 'feeling the true sense of happiness with tea'. With this result taken into serious consideration, this study suggested that more attention and intervention be urgently needed in the field of senior welfare practices, especially leisure activity for elders which is currently in trouble due to the covid-19 crisis. 본 연구의 목적은 주간보호센터 이용 여성노인을 대상으로 차(茶)문화치료 융합프로그램 경험을 탐색하고 그 융합프로그램 경험을 이해하는 것을 목적으로 한다. 이를 위해 P시 H주간보호센터를 중심으로 차(茶)문화치료 융합프로그램 경험을 마지막 회기까지 참여 한 여성노인 4명을 대상으로 심층 면담을 수행하였으며 자료 분석은 Colaizzi의 현상학적 분석 방법으로 하였다. 분석결과 주간보호센터 이용 여성노인을 대상으로 차(茶)문화치료 융합프로그램 경험의 4개의 범주인 되돌림의 일상, 차와 함께 나는 대비마마, 차와 함께 젊은 그대, 차와 함께 행복의 나라로 분석하였다. 차(茶)문화치료 융합프로그램 경험에 대한 분석 결과를 토대로 노인복지실천 영역에서 코비드 19로 인한 노인 여가활동에 관심과 개입이 시급함을 제시하였다.

노인부부의 결혼만족도 향상을 위한 융합프로그램 개발

김인숙,박선희,Kim, In-Sook,Park, Sun-Hee 한국사물인터넷학회 2017 한국사물인터넷학회 논문지 Vol.3 No.2

The purpose of this study was to develop and apply the convergence program to improve the marital satisfaction of the elderly couples. The purpose of this study was to overcome the divorce crisis of elderly couples and to provide a basic plan for divorce prevention through rapid aging and increase of divorce rate. The subjects of this study were 9 elderly women's welfare centers located in P city. The convergence program for improving the marital satisfaction of elderly couples in this study shows that marital satisfaction is improved by 52.1% from pre - test (cumulative complaint of marriage) to 32.1 (post - test). The results of a qualitative evaluation of the observational records show that it is very helpful to improve the relationship by acquiring the skill of sharing experience and communication between the elderly couple through the program, Marital satisfaction was improved. The results of this study show that the convergence program developed in this study is effective in improving marital satisfaction for elderly couples. 본 연구에서는 노인부부들의 결혼만족도 향상을 위한 융합 프로그램을 개발하여 적용하고 그 효과성을 검증하고자 하였다. 이를 통해서 급속한 고령화와 이혼율 증가에 따른 노인부부들의 이혼위기를 극복하고 이혼예방을 위한 기초방안 제공을 목적으로 하였다. 연구대상은 P시에 위치한 S노인복지관 70세 이상의 읽고 쓰기가 가능한 부부 총 9쌍을 대상으로 실시하였다. 본 연구의 노인부부들의 결혼만족도 향상을 위한 융합 프로그램은 사전검사 61.7점(결혼생활 불만누적)에서 사후검사 32.1점(결혼생활 건강)으로 52%의 결혼만족도 향상으로 나타나고 있다. 관찰기록의 한 질적평가의 결과는 프로그램을 통해 노인부부간의 공동체험과 의사소통이 부부관계를 원만하게 하는 기술을 습득하게 하여 부부의 결혼만족도 향상에 도움이 되었다. 그동안 소원했던 노인부부의 관계가 자신이해와 배우자의 이해의 결과로 노인부부의 결혼만족도가 향상되었다. 이상으로 본 연구에서 개발된 융합 프로그램은 노인부부들에게 결혼만족도를 향상시키는데 효과가 있음을 보여주었다.

차문화치료 융합프로그램을 통한 노인의 관계적 공격성 완화 : 노인주간보호센터 이용 여성노인들을 대상으로

김인숙,Kim, In-Sook 한국사물인터넷학회 2020 한국사물인터넷학회 논문지 Vol.6 No.4

본 연구는 관계적 공격성 완화를 위한 차문화치료 융합프로그램의 효과성을 검증하기 위하여 P시 소재 노인주간보호센터를 이용하는 70세 이상의 여성노인들 10명을 연구대상으로 하여 노인의 관계적 공격성 완화를 위한 차문화치료 융합프로그램을 적용하고 그 효과를 살펴보고자 한다. 분석결과로 반응적 관계적 공격성과 주도적 관계적 공격성 점수는 사전검사 평균보다 사후검사 평균에서 0.21 낮아져 차문화치료 프로그램에 참여한 노인들의 주도적 관계적 공격성은 프로그램 참여 전보다 감소 된 결과를 보여주고 있다. 이러한 분석결과를 바탕으로 노인주간보호센터 이용노인의 관계적 공격성 완화를 위한 실천적 제언을 제시하였다. In order to verify the effectiveness of the tea culture treatment program to mitigate the relative aggressiveness, the research will target 10 women aged 70 or older who use the P City-based Senior Citizens' Day Care Center to apply the tea culture treatment program to alleviate the relative aggressiveness of the elderly and examine its effects. As a result of the analysis, the score of reactive relational aggressiveness and leading relational aggression is 0.21 lower than the pre-exam average, indicating that the leading relational aggression of the elderly who participated in the tea culture therapy program is reduced than before the program. Based on these analysis results, practical suggestions were made for alleviating aggression among senior citizens using the Center for the Elderly.