Amygdalin의 In Vitro 분해에 관한 연구

권훈정,조용진 한국독성학회 2007 Toxicological Research Vol.23 No.1

재래식 간장의 제조조건이 ethyl carbamate 생성에 미치는 영향

권훈정,정현정 한국조리과학회 1997 한국식품조리과학회지 Vol.13 No.2

본 연구에서는 식품내의 ethyl carbamate 전구체와 그 형성에 영향을 주는 인자들을 규명하고자 하였다. 일반적으로 제조조건 보다는 원료 메주가 간장의 ethyl carbamate 형성에 기여도가 크나, 같은 메주로 제조한 간장의 경우, 저장 기간에 따라 생성이 증가하며 빛에 의해 그 농도가 감소하는 것이 관찰되었다. 따라서, 가정에서 간장을 담을 경우 전통적인 방법인 빛을 쪼여 주며 보관하는 주의가 필요하다고 사료된다. 원료 메주에 따른 차이는 원료 콩의 화학적 조성도 관여를 하겠으나, 발효에 관여하는 미생물의 종류와 그에 따른 대사과정, 대사량의 차이에 의한 영향도 무시할 수 없으리라 사료된다. 따라서 이러한 기전들을 밝혀내기 위하여 앞으로 연구가 계속 진행되어야 할 것으로 사료된다. Ethyl carbamate is an animal carcinogen and a by-product of fermentation. Since the highest level of ethyl carbamate was found in a traditional soy sauce sample in the previous study, soysauce was prepared in varying experimental conditions with three kinds of meju originated from different area of the country to investigate the factors affecting ethyl carbamate formation. The sample analysis methods included a semi-purification with solid-phase extraction and detection by GC-MS at SIM mode using m/z 62 fragmention. Among the investigated factors, which includes source meju, brining length, salt content, irradiation of visible light during brining or storage, and storage length, source meju and storage length showed most profound effect on the formation of ethyl carbamate. Irradiation of visible light for 12 hours a day during storage lowered the concentration of ethyl carbamate compared to the soysauce kept in the dark. Meanwhile irradiation during the brining as well as the degree of salt contents showed no effect.

보고편 - 제49차 춘계학술대회 ( 주제 : 사회정의와 가정의 역할 ) 분야별 논문발표 - 식생활 분야 : 발효 식품과 Ethylcarbamate

권훈정 대한가정학회 1996 Family and Environment Research Vol.34 No.3

건강 보조 식품과 호르몬 제제

권훈정 한국식품과학회 1997 식품과학과 산업 Vol.30 No.1

A Characterization of Cysteinyl Groups of Rat Liver Metallothionein

권훈정,고은희,신환철,최명언,Kwon, Hoon-Jeong,Koh, Eun-Hie,Shin, Whan-Chul,Choi, Myung-Un 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.3

메탈로사이오닌(Metallothionein, MT)은 다량의 금속과 결합되어 있는 단백질로, 이를 카드뮴을 주사한 쥐의 간으로부터 분리 정제하였다. 정제과정은 열처리, Sephadex G-50 젤 여과법 및 DEAE Sephadex A-25 이온교환 크로마토그래피법을 포함한다. 두개의 isoprotein인 MT-A와 MT-B를 총회 수율 30%로 분리하였다. MT의 금속 결합부위의 화학적 특성을 DTNB (5, 5'-dithiobis(2-nitrobenzoic acid))를 사용하여 검토해 보았다. 이 단백질과 DTNB의 반응은 pH와 이온세기에 민감하게 변화했으며, pH 변화에 따른 pseudo-first order 반응속도 상수는 pH 4 정도에서 최소치를 보였다. 또한 DTNB에 의해 적정되는 MT 내 thiol의 수가 금속의 존재여부나 단백질 구조변화에 따라 상당히 변화함을 알 수 있었으며, 따라서 이 단백질은 적어도 두 가지 종류의 카드뮴-황 결합상태가 존재하리라고 여겨진다. MT (metallothionein), metal-binding protein, was induced with Cd and isolated from rat liver supernatant by heat-treatment, Sephadex G-50 gel filtration, and DEAE Sephadex A-25 ion exchange chromatography. Two isoproteins, MT-A and MT-B, were separated with overall yield of 30%. In order to characterize the chemical properties of metal-binding sites of MT, the cysteinyl groups were examined with DTNB. The reaction of MT-A with DTNB was dependent on the pH and ionic strength. The plot of pseudo-first order rate constant against pH showed a minimum near pH 7.5. It was also found that the DTNB-titrable thiol groups of MT-A were markedly affected by either the presence of metal or by the changes of protein conformation, thus this protein appears to contain at least two different types of cadmium-sulfur bonding.

가열조리방법을 통한 행인 내 시안화합물의 저감화

권훈정,도병경,이동하,나안희,최윤주,이숙연 한국식품위생안전성학회 2007 한국식품위생안전성학회지 Vol.22 No.4

Apricot Kernel, consumed as herbal medicine, contains amygdalin which generate HCN uponhydrolysis. Dyspnea was reported by ingesting large amount of apricot kernel, and neurological disorders such astropic ataxic neuropathy or konzo were known as chronic toxicity of amygdalin. Other cyanogen containing plants,including flaxseed and almond, are consumed as food around the world. Moreover, some of them are promoted asfunctional food, leading to higher consumption, and posing health risk by cyanogenic components. The objective ofthis study was to find a method for the reduction of the cyanogenic compound, using apricot kernel as a model food.The most effective reduction was obtained by boiling the slices of the kernel for one hour in pH 1 HCl solution, show-ing 90% removal. However, the common process known to reduce the cyanogen contents, i.e., long incubation at thelow temperature, did not show significant change in cyan concentration. Our data contribute to the safety of the plantscontaining cyanogenic compounds if they were to be developed as foodstuff.

쥐간 메탈로사이오닌의 시스테인기의 특성연구

권훈정,고은희,신환철,최명원 ( Hoon Jeong Kwon,Eun Hie Koh,Whan Chul Shin,Myung Un Choi ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.3

MT (metallothionein), metal-binding protein, was induced with Cd and isolated from rat liver supernatant by heat-treatment, Sephadex G-50 gel filtration, and DEAE Sephadex A-25 ion exchange chromatography. Two isoproteins, MT-A and MT-B, were separated with overall yield of 30%. In order to characterize the chemical properties of metal-binding sites of MT, the cysteinyl groups were examined with DTNB. The reaction of MT-A with DTNB was dependent on the pH and ionic strength. The plot of pseudofirst order rate constant against pH showed a minimum near pH 7.5. It was also found that the DTNB-titrable thiol groups of MT-A were markedly affected by either the presence of metal or by the changes of protein conformation, thus this protein appears to contain at least two different types of cadmium-sulfur bonding.

이환방향족아민 생성과 돌연변이성에 미치는 단당류와 당알콜의 영향

한인화,권훈정 한국식품과학회 1999 한국식품과학회 학술대회 Vol.1999 No.1

즉석 식품에서 니트로사민이 검출되는 요인 분석

서보경,권훈정 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.2

N-nitrosamines can be produced in the process of heating, processing, storage and packaging. Migration specifications for N-nitrosamines exist only for rubber baby bottle nipples, which are regulated by the Ministry of Food and Drug Safety (MFDS). There is no regulation for other food contact substances (FCS) and studies on N-nitrosamines migration from FCS are rather limited. A pilot study showed an increase in N-nitrosamines contents when cooking instant noodles. Thus, the migration from the packaging was suspected and it was necessary to monitor the migration of N-nitrosamines from food packaging materials and to examine the change in N-nitrosamines contents when cooking instant foods. Three N-nitrosamines, NDMA (N-nitrosodimethtlamine), NDEA (N-nitrosodiethylamine), NDBA (N-nitrosodibutylamine), were analyzed in migration test solutions from plastics such as polyethylene, polypropylene and polystyrene, papers and aluminium containers. In all test solutions, N-nitrosamines were detected less than method quantitation limits (MQLs). Food samples were also investigated to ensure that there is no effect from food contact substances when cooking instant foods. In retort sauces such as curry, black soybean sauce and tomato sauce, NDMA concentration was ranged from 0.54 to 3.81 μg/kg, but there were no significant differences between unheated and heated samples. However, the NDMA contents were significantly increased in most of the instant noodle samples tested when cooked (p < 0.05). No effects from the food contact substances or cooking water was observed. Only when the seasoning powder and noodles were cooked together was NDMA detected. Individual components (noodle, seasoning powder or dried vegetable) or other combinations such as noodles and dried vegetables did not generate N-nitrosamines. Therefore, it is speculated that NDMA may be formed from the precursors in noodles and seasoning powders when they are solubilized in a medium of water.

카드뮴과 납의 다경로 노출량 추정 및 통합 위해성 평가

유창우,권훈정 한국식품위생안전성학회 2020 한국식품위생안전성학회지 Vol.35 No.6

국내외적으로 유해물질의 통합 노출에 대한 관심은 높아지고 있다. 이러한 흐름에 따라 다양한 경로를 통해 노출될 수 있는 중금속에 대한 통합 노출 연구가 필요하다. 카드뮴과 납은 각각 신장 독성과 인지 장애 등 다양한 독성을 나타낼 수 있으며 또한 발암 물질로 알려져있다. 따라서 본 연구에서는 카드뮴과 납의 노출량 추정 및 통합 위해성 평가를 진행하였다. 2016, 2017년의 7기 국민건강 영양조사에 참여한 10,733명의 식품, 물, 흡연과 간접흡연, 호흡, 화장품, 여성용 위생용품을 통한 중금속 노출 추정량을 계산하였다. 결과적으로 카드뮴과 납 모두 식품을 통한 노출이 제일 높게 나타났다. 이외에도 흡연은 카드뮴의 주요한 노출원이었으며, 납은 화장품을 통해 높은 농도로 노출되었다. 통합 위해평가에서도 식품이 가장 큰 영향을 미쳤다. 지역적 특성의 차이는 노출 추정량의 차이를 보이지 못하였으나, 연령 별, 성별 간 노출 추정량은 큰 차이를 보였다. 특히, 월경 중인 성인 여성의 경우 카드뮴, 월경 중이지 않은 여성은 납의 노출 추정량이 더 높으며 신체 대사를 고려하였을 때, 그 위험성이 더 클 수 있음을 암시하였다. 결론적으로 노출량 추정 및 통합 위해평가 모두 식품이 주요 노출원이었다. 다만, 잠재적 위험을 방지하기 위해 다른 경로에 대한 노출량 추정 및 위해평가가 요구된다. Exposure to hazardous substances occurs through multiple pathways. Aggregated risk assessment, which includes all potential exposure pathways to a single toxicant, is necessary to prevent exposure to harmful substances. We aimed to estimate cadmium and lead exposure through various media, such as food, water, air, smoking, cosmetics, and female hygiene products. This study covered 10,733 subjects from the Seventh Korea National Health and Nutrition Examination Survey(2016, 2017). Dietary exposure was estimated using 24-hour recall data. For water and inhalational exposure, regional variations were considered. Water was classified as tap, bottled, and public water. Inhalational exposure was estimated using the ‘2014 Time Use Survey’ based on daily lifestyle and social status. The frequency and volume of cosmetic usage were randomly approximated by sex and age. Post-menarcheal and premenopausal women were assumed to use feminine hygiene products. Non-carcinogenic aggregated risks were estimated using the Aggregate Risk Index from EPAs and the Total Exposure Hazard Index from Korean government guidelines. For carcinogenic risk assessment, excessive cancer risk was estimated. Ingestion, especially food, was the major route for both cadmium and lead exposure. Smoking was also associated with high cadmium exposure. Exposure to lead from cosmetics was remarkable but not critical. In aggregate risk assessments, median cadmium and lead exposure did not exceed the reference value. Sex, age, smoking status, and income affected exposure levels, unlike to regional variations.