High-throughput genetic screens using CRISPR–Cas9 system

권지연,김용섭 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.9

The CRISPR–Cas9 system is a powerful tool for genome engineering, and its programmability and simplicity have enabled various types of gene manipulation such as gene disruption and transcriptional and epigenetic perturbation. Particularly, CRISPR-based pooled libraries facilitate high-throughput screening for functional regulatory elements in the human genome. In this review, we describe recent advances in CRISPR–Cas9 technology and its use in high-throughput genetic screening. We also discuss its potential for drug target discovery and current challenges of this technique in biomedical research.

포스터 전시 발표 순서 : 소화기 ; 급성 A형 간염 환자에서 발생한 용혈성 빈혈 1례 (초)

권지연,김영석,김상균,장재영,정승원,김홍수,문종호,이문성,심찬섭,김부성 대한내과학회 2008 대한내과학회지 Vol.75 No.1S

신앙의 모양만 구하다 잃어버린 것들 : 코로나가 드러낸 한국개신교회 민낯

권지연 우리신학연구소 2021 가톨릭 평론 Vol.31 No.-

Transcending Worldliness: A Wagtail by Lakeside by Jo Sok (1595-1668)

권지연 한국학중앙연구원 한국학중앙연구원 2017 THE REVIEW OF KOREAN STUDIES Vol.20 No.2

Cho Sok (1595-1668) remains an unmatched bird painter in ink of literati background from the Joseon dynasty (1392-1910). Today, over 57 paintings in private and public collections around the world bear the seal of his penname, Changgang. This article focuses on two newly surfaced wagtail paintings in ink in San Francisco, arguing that such depictions of solitary birds in ink actually form the mainstream of his bird paintings. While positioning them within the stylistic and iconographic framework of his known works, it also explores their possible meaning through the psychological lens of the artist and the historical context of the period. A witness to the major historical events in seventeenth-century Korea, one of the most tumultuous times in Korean history noted for factional politics and foreign invasions under the shifting paradigms of the East Asian world order, his bird paintings encapsulate, as symbols of his unspoken words, the abiding ideals of a disheartened Neo-Confucian scholar.

급성 A형 간염 환자에서 발생한 용혈성 빈혈

권지연,김영석,김상균,장재영,정승원,김홍수,문종호,이문성,심찬섭,김부성 대한내과학회 2008 대한내과학회 추계학술대회 Vol.2008 No.-

Reverse Transcription Polymerase Chain Reaction-based System for Simultaneous Detection of Multiple Lily-infecting Viruses

권지연,최선희,류기현 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.3

A detection system based on a multiplex reverse transcription (RT) polymerase chain reaction (PCR) was developed to simultaneously identify multiple viruses in the lily plant. The most common viruses infecting lily plants are the cucumber mosaic virus (CMV), lily mottle virus (LMoV), lily symptomless virus (LSV). Leaf samples were collected at lily-cultivation facilities located in the Kangwon province of Korea and used to evaluate the detection system. Simplex and multiplex RT-PCR were performed using virus-specific primers to detect singleor mixed viral infections in lily plants. Our results demonstrate the selective detection of 3 different viruses (CMV, LMoV and LSV) by using specific primers as well as the potential of simultaneously detecting 2 or 3different viruses in lily plants with mixed infections. Three sets of primers for each target virus, and one set of internal control primers were used to evaluate the detection system for efficiency, reliability, and reproducibility.

‘3·1운동 및 대한민국 임시정부 100주년 특별전’ : 전시방법과 매체

권지연 대한민국역사박물관 2019 현대사와 박물관 Vol.2 No.-

올해 3·1운동 및 대한민국임시정부 100주년을 기리면서 서울시 소재 여러 박물관에서는 다양한 관점과 주제로 특별전을 개최했다. 본고는 국립중앙박물관, 서울역사박물관, 서대문형무소 역사전시관, 그리고 고양시에 위치한 국립여성사전시관의 특별전들을 관람하고 이 중대한 역사적인 변환기를 서술하는데 활용한 방법과 매체들을 살펴보았다. 다양한 각도에서 접근한 이 전시들은 역사적 가치가 있는 일차적인 유물 외에도 이들을 둘러싼 상황을 만화, 공간 재현, 인터렉티브 미디어아트, 사진 설치작업, 동영상, 상황 재연 비디오, 디지털아트 등을 활용하여 교육적인 체험과 현 시점에서 의의를 찾고 기념하는 활동을 유도하였다.

급성 A형 간염 환자에서 발생한 용혈성 빈혈 1례

권지연,이지은,김영석,김상균,문종호,이문성,심찬섭,김부성 순천향의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.14 No.3S

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활동 및 자료형태가 중학생의 영어 쓰기, 연결어 사용 및 태도에 미치는 영향 : 중학교 2학년을 중심으로

권지연 이화여자대학교 교육대학원 2006 이화교육논총 Vol.16 No.-

The importance of communicative competence in English has been emphasized much more than before and recent English curriculum has focused on developing practical English skills in this era of globalization. Korean students have a need to improve their English writing skill. However, it is difficult for students to find opportunities to write in English both in the classroom and outside the classroom. Recent research has shown that writing through communicative activities is effective at improving not only the communicative competence, but general English abilities. It has also shown that the types of materials, picture or word, in writing have important effects on the learner writing. Therefore, this study is mainly concerned with types of materials and writing activities in EFL writing classes. This study aims at examining the effect of writing activities and materials on the English writing abilities, the use of connectives and the attitudes of students after training. For the purpose of this study, the experiment was carried out over 8 weeks using 280 Korean middle school students in 2nd grade. Students were divided into three groups, which were Jigsaw A, Jigsaw B and individual group, according to the types of writing activities and each group is divided again into the picture and word group according to the types of materials. Jigsaw A is a two-way information gap activity including both expert and home groups. Jigsaw B is also a two-way information gap activity, but excluding the expert groups. During the writing training, students wrote additive and adversative writings, sequential writings and causal writings as well as pre- and post-writing. Data for the research was collected from students' writings from groups and individuals, the evaluations for students' understanding of connectives, classroom observations, students' questionnaires and interviews. According to the results of the study, we were able to identify several trends. First, the types of writing activities showed meaningful differences of the writing abilities except additive and adversative writings, It is expected that more negotiations of meaning through 2 phases of group activities, expert and home group, resulted in these differences. In contrast, the types of writing activities didn't show meaningful differences of the use of connectives. So it was believed that Jigsaw A activities including expert and home groups had a positive effect on the writing abilities. Second, the effect of types of materials did not show meaningful differences from comparing pre- and post-evaluation of connectives. However, the analysis of the frequency of connectives in each text proved meaningful differences according to the types of materials. The picture materials in sequential writings and causal writings had positive effects on the frequency of connectives. Therefore, it is expected that the pictures made learners understand the whole story more easily and improve the abilities of organizing a story. Third, the types of writing activities and materials had a positive influence on the students' attitude about English writing, cooperative writing and cooperative learning as well as motivation and confidence of writing. It is expected that Jigsaw A groups were particularly effective in their writings and showed more confidence through the training. Finally, there were certain differences from classroom observations, students' questionnaires and interviews. There were different kinds of writing features between writings from pictures and those from words. From students' interviews, higher level students in Jigsaw A and Jigsaw B felt more responsibilities as leaders of their own group. However, lower level students were inclined to depend on higher level students, It is expected that the types of writing activities and materials have different effects according to the level of students. In conclusion, the results of this study have shown that the types of writing activities and materials result in the meaningful differences, In particular, jigsaw A groups and picture groups make positive effects on student writings and the use of connectives, In addition, Jigsaw activity including an expert group and a home group is expected to be more helpful for those students who have few writing opportunities in EFL classrooms. However, high level students felt burdened as leaders in jigsaw group, so the teacher should manage those problems by encouraging low level students. For the material, pictures should be selected as meaningful inputs to students to make them interact more actively. Therefore, more helpful instructions to encourage more interaction between students, are required for effective writing activities.

장알균 균혈증의 임상적 특징

권지연,김태형,이은정,전민혁,추은주,박연희,이상헌,박혜란,박혜원,박희순,배성필,백애린 순천향대학교 순천향의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.15 No.2

Background : Enterococci have been regarded as pathogen of low grade virulence. But they have emerged as an increasingly important cause of nosocomial infections in the last decade. The most common nosocomial infections produced by these orgnisms are urinary tract infections, followed by intra-abdominal and pelvic infections. This study was conducted to clarify the clinical features and outcome of patients with enterococcal bacteremia. Methods : Patients with enterococcal bacteremia treated at a Soonchunhyang university hospital in Seoul between January and June 2008 were reviewed. Results : Twenty three patients (14 males and 9 females) were included for analyses. The mean age was 64 years (34~83). The risk factors of enterococcal bacteremia were diabetes mellitus (47.8%), solid cancer (34.8%) and catheter insertion status (34.8%). The portal of entry included biliary tract (34.8%), urinary tract (17.3%) and respiratory tract (13.4%). Bacteremia was caused by E. faecalis in 13 patients and by E. faecium in 8 patients. E. faecium showed a higher rate of resistance to ampicillin than E. faecalis. 11 patients received therapy and the most common monotherapy was vancomycin (21.7%). But 7 patients (30.4%) didn't received therapy, moreover 6 patients (26.1%) continued with antibiotics resistant to enterococcus (cephalosporins). A total 5 of 23 patients (mortality : 21.7%) had died and all of them died from underlying disease. Conclusions : Knowledge of the characteristics of enterococcal bacteremia may be useful to improve the empirical treatment.