http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
권길광(Kil Koang Kwon),윤석민(Seok Min Yoon),최창호(Chang Ho Choi),정봉근(Bong Geun Jeong),이기원(Ki Won Lee),이동희(Dong Heui Yi),김형주(Hyung Joo Kim) 大韓環境工學會 2007 대한환경공학회지 Vol.29 No.6
본 연구에서는 개인용 컴퓨터(PC) 내부의 시료를 채취하여, 세균의 CFU와 곰팡이의 종류를 분석하였다. 시료는 대중 PC 이용시설(PC방), 대학실험실 및 대학 전산실습실의 PC 51대에서 채취하였다. CFU(colony forming unit)법을 이용한 세균수의 경우, 컴퓨터 총 작동시간이 증가할수록 PC 내부의 세균수는 증가하는 것으로 확인되었으며(r2 = 0.90), PC 내부 부품 중에서는 CPU 냉각 팬에서 가장 높은 수치로 확인되었다(평균 605 CFU/cm2). 곰팡이의 경우, 다수의 유해성을 지닌 곰팡이가 검출되었으며, 그 중 Aspergillus sp.와 Penicillium sp.가 가장 많은 비율로 존재함이 확인되었다. 또한 PC 내부에서 채취된 먼지에서는 mg 당 212 CFU의 세균이 발견되었다. 본 연구의 결과, PC 내부에는 여러 다양한 종류의 미생물이 존재한다는 것을 확인하였으며, 이 결과는 PC의 취급, 사용 및 정비 시 각종 미생물에 의한 감염의 가능성이 있다는 것을 나타내고 있다. Presence and distribution of bacteria and fungi in inner compartment of PCs(Personal Computers) were investigated. Samples for the analysis were collected from inside of PCs which had been used in various facilities including public computer facilities, laboratories and computer training rooms of a university. Total number of PC examined in this study was 51 each. When the total CFU(colony forming unit) of the inner compartment of the PCs was measured, the bacterial count was found to be dependent on the operation time(total running time) of PCs. When the distribution of bacteria in the inner compartment of PCs was estimated, CPU(Central Processing Unit) cooling fan area showed the highest bacterial concentration(average 605 CFU/cm2). In the case of the fungi, various opportunistic pathogens including Aspergillus sp. and Penicillium sp. were isolated and identified in the inner compartment of PCs. And the average of bacterial number in the dust collected from the PCs was 212 CFU/mg. These results indicated that handling of PC might have a risk of infection by the microorganism.
김형주,권길광,서규원,김민철,김형섭,고준석,Kim, Hyung-Joo,Kwon, Kil-Koang,Seo, Kyu-Won,Kim, Min-Chul,Kim, Hyung-Sup,Koh, Joon-Seok 한국섬유공학회 2009 한국섬유공학회지 Vol.46 No.6
In this study, microbial degradation of lignin and pectin in raw hemp was investigated. Using lignin and pectin containing media, various microorganisms were isolated from soil and fecal samples of ruminant. The isolates were identified using the 16s rRNA method. The isolated strains were Klebsiella sp., E. coli, Aspergillus fumigatus, and Burkholderia sp. For the degradation of hemp, a pre-cultured isolate was inoculated (5% v/v) into the medium containing raw hemp (as a sole carbon source: 1% w/v) and nitrogen sources and the mixture was incubate aerobically for 168 hours in a shaking incubator (200 rev $min^{-1}$) at $30^{\circ}C$. The hemp biodegradation activity of the identified microorganism was measured by FT-IR. The FT-IR results showed that the Klebsiella sp. has the highest lignin and pectin degradation activity. These results show that the isolates and the reaction conditions could be applied to the conventional hemp fiber refinery processes.
박수빈,Quynh-Giao Tran,류애진,윤진호,권길광,이용재,김희식 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.4
Ceramides are a major lipid class known to play an essential role in maintaining skin function. Thus, effortshave been made to produce ceramides and ceramide precursors in large quantities for industrial applications. The yeastWickerhamomyces ciferrii, a natural producer of the ceramide precursor tetraacetyl phytosphingosine (TAPS), has beenisolated and engineered through various mutagenesis approaches aiming to enhance TAPS production. Herein, a highthroughputscreening platform for isolating W. ciferrii mutants with improved TAPS production is described. A fluorescence-mediated reporter system that allows initial quantification of TAPS content in yeast cells based on BODIPYstaining was developed. The optimal concentration of BODIPY for monitoring intracellular TAPS levels in W. ciferriiwas 400 g/L, as shown by a linear correlation between the actual TAPS levels and mean fluorescence intensities. Fluorescence-activated cell sorting was used for isolating high TAPS-producing strains from an ethyl methanesulfonateinducedmutant library. After several rounds of sorting, mutants exhibiting a high-TAPS phenotype were isolated, andthe M40 strain with the highest TAPS titer was chosen for large-scale cultivation. The influence of different carbonsources for optimizing TAPS production was also evaluated using the M40 strain. A maximum production yield of5.114 g/L of ceramide precursors, including TAPS and triacetyl phytosphingosine, was achieved with the supplementationof molasses. This novel platform enables rapid screening of high TAPS-producing strains using the common dyeBODIPY and can be easily extended for the development of mutants with high productivity of ceramide precursors inyeast and other microorganisms.