체외수정 시술 후 발생된 병합임신 1 례

박기영(Ki Young Park),이영(Young Lee),송지민(Ji Min Song),류진희(Jin Hee Yoo),박철훈(Cheol Hoon Park),고영미(Young Me Koh),김홍기(Heung Ki Kim),김창이(Chang Yee Kim) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.8

The simultaneous existence of intrauterine and extrauterine pregnancies is known as a heterotopic pregnancy. Spontaneous heterotopic pregnancy is a rare event although its incidence has increased since the recent development of treatment of infertile women with ovulation induction or in-vitro fertilization and embryo transfer(IVF-ET).The theoretical rate of this condition was estimated to be approximately 1 in 30,000 pregnancies. The early diagnosis of heterotopic pregnancy is very difficult . So there is a high maternal morbidity and fetal loss. We reported a IVP - ET patient resulting in the successful delivery of live infant at 35weeks of gestational age from intrauterine pregnancy following surgical removal of ruptured concurrent extrauterine pregnancy.

태아 폐성숙 평가에 있어서 Lamellar Body Count의 임상적 효용성과 경제성

홍승표 ( Seung Pyo Hong ),박은경 ( Eun Kyung Park ),정선영 ( Sun Young Jeong ),주하경 ( Ha Kyong Joo ),이지현 ( Jee Hyun Lee ),문희봉 ( Hee Bong Moon ),고영미 ( Young Me Koh ),신종철 ( Jong Chul Shin ),김창이 ( Chang Yi Kim ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.11

목적: 신생아 폐성숙을 예측하기 위하여 시행하는 Lamellar body count의 유용성과 경제성을 알아보고자 하였다. 연구 방법: 폐성숙도 평가를 위하여 양수천자를 시행한 후 72시간 내에 분만한 32명의 산모를 대상으로 하였다. Lamellar body count는 경복부 양수천자를 통해 얻어진 양수에서 깨끗한 양수는 바로 검사를 시작하고 혈액이나 태변에 오염된 양수는 3분간 원심 분리한 후 3개의 sample로 나누어서 연속적으로 Coulter Objective: To evaluate the availability and efficacy of the Lamellar body count as a predictor of fetal lung maturity Methods: Amniocentesis was performed for evaluation of fetal lung maturity status within 72 hours of delivery in 32 patients. A Lamellar

접촉성 Neodymium: Yttrium Aluminum Garnet(Nd: YAG) 레이저를 이용한 토끼 자궁각문합술

전준연 ( Joon Yeon Jun ),정선안 ( Sun An Jung ),송영훈 ( Young Hun Song ),고영미 ( Young Me Koh ),김장흡 ( Jang Heub Kim ),김진홍 ( Jin Hong Kim ),이진우 ( Jin Woo Lee ),김수평 ( Soo Pyung Kim ) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12

This study was aimed to determine the feasibility of using the Nd: YAG laser with a synthetic sapphire contact tip to weld the transected rabbit uterine horns and to compare the effects of conventional microsurgical anastomosis with Nd: YAG laser welding of the uterine horns. Forty -eight rabbit uterine horns were transected and subjected to microsurgical anastomosis(control group, 16 uterine horns) or laser welding(laser group, 32 uterine horns). The laser group was divided into 3 subgroups based on the different laser power levels; 4, 5, and 6 watts level were applied to 8, 8, and 16 uterine horns, respectively. The surgical time of each procedure were recorded. Four weeks later, a laparotomy was performed to determined adhesion formation and uterine horn patency, and a histologic examination was done of the anastomosis sites. The results were as follows: 1. In the case of a unilateral uterine horn anastomosis, control group required significantly longer operation time(30.2±2.1 minutes) than 4, 5, or 6 watts laser group(9.3 ± 1.5, 8.2 ± 1.3, 8.5 ± 0.7 minutes, respectively). 2. Laser group produced slightly less adhesion(adhesion score, 1.09±0.78) than control group(l.44 ±0.96), but there was no significant difference. 3. One of 16 uterine horns in control group(6.3%) and one of 32 in laser group(3.l%) were not patent by chromopertubation using methylene blue. The leakage of methylene blue at the anastomosis sites were 6.3% in control group and 12.5% in laser group. Thus, the patency rate(without occlusion and leakage) were 87.5% in control group and 84.4% in laser group, whose result does not show significant difference. Meanwhile, laser group with 6 watts power level showed 1000%patency rate. 4. There were fewer constrictions at the anastomosis sites in laser group than control group in some rabbits. In other rabbits, the uterine homs were equally smooth. Microscopically, well-healed mucosal layer and minimal inflammation at the anastomosis aites were observed in laser group, but there were irregular mucosal layer, thin and mildly disrupted muscularis layer with some fibrosis, moderate inflammation, and foreign body granulomas were found in control group. 5. The contact Nd: YAG laser with a synthetic sapphire tip was found to be able to weld the transected rabbit uterine homs successfully. The most appropriate power level was 6 watts in the experiment. In conclusion, the patency rates were similar between the control and Nd: YAG laser group. But less operation time and simple manipulation of Nd: YAG laser might enable clinicians to get a better results on the tubal reconstruction.

Cisplatin 내성 난소암 세포주 Clone의 DNA Mismatch Repair 유전자 변이및 Taxol, Topotecan의 민감도

김흥기(Heung Ki Kim),손우석(Woo Seok Son),박태철(Tae Chul Park),김태응(Tae Eung Kim),고영미(Young Me Koh),송지민(Ji Min Song),박기영(Ki Young Park),김재훈(Jae Hoon Kim),유기성(Ki Seong Ryu),김진우(Jin Woo Kim),남궁성은(Sung Eun NamKoo 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.11

목적: 부인 종양에서 가장 심각한 문제중 하나인 난소암은 항암화학요법에 비교적 민감하게 반응하는 고형 종양이나 다양한 노력에도 불구하고 사망률은 큰 변화가 없는 실정이다. Cisplatin은 이들 항암제의 중심이며 가장 효과적인 약제이지만, Cisplatin에 대한 약제내성의 출현은 치료 실패의 중요한 원인이 되고 있고, 최근 Hereditary nonpolyposis colorectal cancer에서 입증된 DNA 부정합 보수 유전자의 변이가 여러 약제에 대하여 약제내성을 초래한다는 사실이 밝혀지고 있다. 이에 따라 Cisplatin 내성을 갖는 난소암 세포주에서 DNA 부정합 보수 유전자인 hMLH1과 hMSH2의 돌연변이와 Microsatellite instability 여부를 알아보고, 약제내성을 갖는 난소암 세포주에서 얻은 단일 세포의 clone들중 부정합 보수 유전자에 변이가 있는 clone과 정상인 clone을 분류하여, 이들에서 난소암의 2차 약제로 많이 쓰이는 Taxol, Topotecan의 항암제 민감도에 차이가 있는지 밝히고자 연구를 시행하였다. 연구방법: 상피성 난소암 세포주 2008과 이 세포주에 Cisplatin의 농도를 반복적으로 증가시켜 만들어진 2008/C13*5.25 세포주에서 추출한 cell clone으로 hMLH1과 hMSH2 단백질로 immunoblot을 시행하고 각각의 clone에 대하여 taxol 및 topotecan의 colony forming assay를 이용하여 항암제 민감도 검사를 시행하였다. 결과: 1. 2008 세포주와 2008/C13*5.25 세포주에서 hMLH1과 hMSH2에 대한 항체를 이용하여 immunoblot을 시행한 결과 각각의 세포주에서 모두 hMLH1과 hMSH2 단백질의 발현을 확인 할 수 있었다. 2. 2008 세포주에서 hMLH1(+)인 clone은 78개, hMLH1(-)인 clone은 22개 였고, 2008/C13*5.25 세포주에서는 hMLH1(+)인 clone은 73개, hMLH1(-)인 clone은 27개로, hMLH1(-)로의 변이는 2008/C13*5.25에서 약간 많았지만 통계적으로 유의한 차이는 없었다. 3. 2008과 2008/C13*5.25 세포주에서는 Taxol에 대한 민감도에 차이가 없었다. 그러나 2008/C13*5.25 hMLH1(-)의 clone들이 hMLH1(+)인 경우 보다 유의하게 Taxol에 민감하였고(P=0.049), 2008 hMLH1(+)인 clone들은 hMLH1(-) clone보다 유의하게 Taxol에 민감하였다(P=0.003). 4. 2008과 2008/C13*5.25 세포주에서는 Topotecan에 대한 민감도에 차이가 없었고. 2008/C13*5.25 hMLH1(-)의 clone들이 hMLH1(+)보다 유의하지는 않았지만 Topotecan에 약간 민감하였고, 2008 세포주에서는 hMLH1(-) clone들이 hMLH1(+)보다 유의하게 Topotecan에 민감하였다(P=0.001). 또한 2008과 2008/C13*5.25 두 세포주에서 hMLH1(-)인 clone들은 cisplatin 내성에 관계없이 유의하게 Topotecan에 민감하였다(P=0.001). 5. BAT25, BAT26, BAT34 primer에 대한 2008 hMLH1(+), hMLH1(-) clone과 2008/C13*5.25 hMLH1(+), hMLH1(-) clone의 microsatellite instability 결과는 모두 정상의 allele 소견으로 microsatellite instability를 나타내지 않았다. 결론: 이상의 연구 결과로 미루어 난소암에서 DNA 부정합 보수 유전자의 변이와 Cisplatin의 약제내성 사이에는 뚜렷한 관련이 없어 보이고, 난소암에서 hMLH1의 소실이 있는 경우는 오히려 Taxol이나 Topotecan에 민감한 것으로 나타났으며, 이러한 결과는 난소암 치료에서 1차 및 2차 약제의 선택에 도움을 줄 것으로 사료된다. Objective: Ovarian cancer represents a relatively chemosensitive solid tumor, with responsiveness to a range of agents. Cisplatin is the mainstay of drug treatment and is one of the most active single agent. However, the overall outcome for patients remains unsatisfactory and the emergence of drug resistance is a major factor in treatment failure. Loss of DNA mismatch repair is a common finding in many types of sporadic cancer as well as in patients with hereditary nonpolyposis colon cancer. Cells that lack DNA mismatch repair are resistant to commonly used chemotherapeutic agents. Selection of cells for resistance to cisplatin, a well-recognized mutagen, could result in mutation in genes involved in DNA mismatch repair. Methods: This study evaluated the mutation of hMLH1 and hMSH2, and its relation to the Taxol and Topotecan chemosensitivity in the clones from the ovarian cancer cell line 2008 and cisplatin-resistant cell line 2008/ C13*5.25. Results: 1. Cells from 2008 and 2008/C13*5.25 expressed both hMLH1 and hMSH2 when analysed with immunoblotting. 2. Twenty two out of 100 single-cell clones from 2008 and 27 of clones from 2008/C13*5.25 expressed no hMLH1. hMSH2 was expressed in all clones. 3. There was no difference of Taxol chemosensitivity between 2008 and 2008/C13*5.25 cell lines. In the 2008/C13*5.25 cell line, the hMLH1-deficient clones were more sensitive to Taxol than the hMLH1-proficient clones(P=0.049), but in 2008 cell lines hMLH1-proficient clones were more sesitive to Taxol(P=0.003). 4. There was no difference in Topotecan chemosensitivity between 2008 and 2008/C13*5.25 cell lines. In the 2008/C13*5.25 cell line, the hMLH1- deficient clones were not more sensitive to Topotecan than the hMLH1-proficient clones. In the 2008 cell lines hMLH1-deficient clones were more sesitive to Topotecan(P=0.001). Overall, hMLH1-deficient clones from both 2008 and 2008/C13*5.25 cell lines were significantly more sensitive to Topotecan(P=0.001). 5. Microsatellite instability was not demonstrated in all 4 types of single-cell clones from 2008 and 2008/C13*5.25 cell lines. Conclusions: The present results indicate that there is no relation between mutation of mismatch repair gene and cisplatin resistance. But hMLH1-deficient ovarian cancer cells are more sensitive to Taxol or Topotecan in this study. The latter finding mandates the examination to assess the mutation of hMLH1 in tumor cells before treatment or at the time clinical resistance to cisplatin develops in ovarian cancer.

원발성 난관 융모막암종 1 예

이은주(Eun Ju Lee),이정기(Jeong Ki Lee),홍승표(Seung Pyo Hong),민기옥(Ki Oak Min),문희봉(Hee Bong Moon),고영미(Young Me Koh),김창이(Chang Yee Kim),김흥기(Heung Ki Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

Primary choriocarcinoma of the fallopian tube has been known for 4% of choriocarcinoma also 1.7% of gestational trophoblastic disease. Its symptom and sign in presentation are similar to the ectopic pregnancy or adnexal mass, thus it is confirmed through histopathological descriptions after explolaparotomy or laparoscopy. Mostly it is common in younger women who are reproductive, we have done conservative surgery followed by chemotherapy. After that, the prognosis was good. We have experienced a case of primary choriocarcinoma of the fallopian tube and reported with a brief review.

질 원개 탈출증에서 엉치 가시 인대 질 고정술의 효용성

이정원 ( Chung Won Lee ),김정 ( Jeong Kim ),김수연 ( Sue Yeon Kim ),천소희 ( So Hee Cheon ),장동규 ( Tong Gyu Chang ),최주혁 ( Joo Hyuk Choi ),서경윤 ( Kyoung Yun Seo ),문희봉 ( Hee Bong Moon ),고영미 ( Young Me Koh ),김창이 ( Ch 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.1

산전 진단된 신경아세포종 1 예

이정기(Jeong Ki Lee),이은주(Eun Ju Lee),홍승표(Seung Pyo Hong),김지훈(Ji Hoon Kim),최운민(Woon Min Choi),민기옥(Ki Oak Min),문희봉(Hee Bong Moon),김흥기(Heung Ki Kim),김창이(Chang Yee Kim),고영미(Young Me Koh) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.11

We report a case that a neuroblastoma in a fetus was recognized before birth and its growth could be observed. The diagnosis was made by ultrasonography. The suprarenal mass initially showed pure cystic features on ultrasound. Surgical exploration revealed an adrenal cystic tumor and histology showed a neuroblastoma in situ. Forty-five infants with prenatally detected neuroblastoma were found in the English literature; about one-half of them were cystic neuroblastomas and most had a favorable outcome.

여성 생식기 암에서 c-K-ras 암유전자의 점 돌연변이 검출

김승조,이필호,박종섭,남궁성은,김휘,고영미,김홍기,한상균 대한부인종양 콜포스코피학회 1994 Journal of Gynecologic Oncology Vol.5 No.2

lt has been well established that, specifi alterations in members of the ras gene family, H-ras, K-ras and N-ras, can convert them into active oncogenes. These alterations are either point mutations occurirg in either codon 12, 13 or 61, or alternatively, a 5- to 50-fold amplification of the wfld-type gene. Activated ras oncogenes have been found in a significant proportion of all turnors, but the incidence varies considerably with the tumor type : it is frequent (20~40% ) in colarectal eancer and acute myeloid leukemia, but absent or preaent rarely in breast and atomach cancer. But the role of c-K-ras point mutatio in the development of cancers in the female genital tract has not been extensively studied. Polymerase chain reaction followed by gel electrophoresis was performed respectively using wild-type normal and specific point mutation primers{GGT-$gt;GAT, GGT-$gt;AGT, GGT-$gt;TGT and GGT-$gt;GTT) to detect, point, mutation of codon 12 of c-K-ras oncogene. The c-K-ras oncogene point mutation was confirmed by Southern blot hybridization using synthetic oligonucleatide probe. 3'-end Iabelled with digoxigenin -dUTP. With this method, the frequency of point mutation on codon 12 of c-K- ras oncogene was examined the tissues in 37 casea of ovarian cancer, 7 cases of endometrial cancer, 36 cases of the gestational trophoblastic tumor, 60 cases of cervicaI cancer. The relationship between the presence of a c-K-ras point mutation and clinicppathologi al characteristics of the female genetial tract cancers were also analysed The results were as follows; 1. The incidence of four point mutations on codon 12 of K-ras oncogene in 37 ovarian cancers was 45.9% (17/37) and distribution were 43.2%(16/37), 2.7%(1/37), 0%(0/37) and 0%(0/37) in GGT�GAT, GGT�AGT, GGT�TGT, and GGT�GTT, respectively According to histological type, in ovarian cancers, the point mutation of K-ras oncogene waspositive in 45% (10/22) of serous cystadenocarcinomas. The incidence of four point mutations on codon 12 among 37 patients with ovarian cancer according to histological type was 45.5% (10?22) with serous cystadenocarcinoma, 57.1% (4/7) of mucinous cystadenocarcinoma. Comparing the positive rate of point mutations of K-ras oncogene among 37 patients with ovarian cancer with the clinical stage, point mutation was detected in 28.5%(2/7) or patients with stage, I. 40.0%(2/5) with stage II. and 52.0%(13\/25) with stage III/IV, there was no statistically significant increasement of point mutaiions with the advance of the clinical stage of ovarian cancer. Comparing the positive rate of point mutations of K-ras oncogen among 37 patients with ovarian cancer according to the histologic grade, point mutation was detected in 50.0%(2/4) of patients with grade I, 41.7%(5/12) with grade II and 47.6%(10/21) with grade III. 2. The incidence of point mutations of K-ras oncogen among 33 patients with ovarian cancer who were performed pelvic lymph nocd dissection was 57.15, (12/21) of the patients with pelvic lymph node metastases and 16.7%(2/12) of the patients without pelvic lymph node metastases. There was statistically significant difference between the positive rate of c-K-ras point mutations and the pelvic lymph nodal status(P$lt;0.05). 3. In 7 cases of endometrial cnacer, poistive rate of K-ras point mutation was 42.8% (3/7). point mutations were also detected in 2 cases from 4 choriocarcinomas, but, the point mutation was only detected in 1 case from 60 cervical carcinomas From thses results, we may suggest that the point mutation on codon 12 of c-K-ras oncogene are considered to be one of the important genetic change in the tumor formation and progression of ovarian cancers. the activation of c-K-ras oncogene seems to be the on step in the multistep process of tumor formation in ovarian cancer, furthermore, the point mutation of c-K-ras gene could occur more frequently in the patients of ovarian cancer with plevic lymph node metastases than in those without pelvic metatases, suggesting the role in tumor progression. And we concluded that point mutation on codon 12 is comparable frequent in uterine endometrial carcinomas and may have significance as an event that contributes to progrrssion of endometrial cancers and choriocarcinoma, but cervical carcinoma do not appear to have c-K-ras point mutation in general. More studies will be necessary, but the detection of c-K-ras point mutationas the possibility of biological tumor marker to predict clinical outcome may be utilized in female malignancies

침윤성 기태의 초음파 소견

서경윤,진동근,최주혁,천소희,장동규,민기옥,문희봉,고영미,김창이 대한산부인과초음파학회 2003 대한산부인과초음파학회지 Vol.5 No.2

한국 여성 자궁경부 종양 환자에서 인유두종 바이러스 유전자형 분석

박태철,김찬주,이근호,윤주희,김지훈,고영미,남궁성은,박종섭 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.1

인유두종 바이 러스(human papillomavirus; HPV)는 자궁경부암의 발생 에 있어서 가장 중요한 원인으로 인정되고 있으며, 자궁경부 세포진 검사에 대해 보조적인 방법으로 HPV 진단법의 효용성이 확인되었다. HPV 백신의 개발이 예상되고 있는 시점에서 한국 여성에서 HPV 유전자형 분포도에 대한 지식은 자궁경부암의 진단과 치료와 예방에 있어서 매우 중요할 것으로 생각된다. 2001년 11월부터 2002년 5월까지 가톨릭대학교 의과대학 강남 성모 병원 산부인과를 방문한 외래 및 입원 환자를 대상으로 하여, 자궁경부 세포진 또는 병리 조직 검사 소견에 따른 HPV 유전자형의 분포를 분석하였다. 환자들의 평균 연령은 43.4세이며, 21세에서 91세의 범위에 있었다. 자궁경부 세포진 또는 병리 조직 검사 소견에 따라 정상 세포진 소견을 보이는 여성(n=290), LSIL (low-grade squamous intraepithelial lesion) 세포진 검사와 일치하는 조직 검사 결과로 HPV 감염 또는 경증 상피이형증으로 나타난 환자(n=68), HSIL (high-grade squamousintraepithelial lesion) 세포진 검사와 일치하는 조직 검사 결과로 중등도 상피이형증, 중증 상피이형증 또는 상피내암으로 나타난 환자(n=51), 자궁경부암 환자(n=55)로 분류하였으며, HPV DNA chip을 이용하여 각 군에서 채취한 자궁경부 세포에서 HPV의 존재를 파악하고 유전자형 분포와 복합 감염을 관찰하였다. 각 군당 HPV 발견율은 정상군 290명 중 51명(17.6%)에서 HPV 양성이었으며, LSIL에서 68명 중 50명(73.5%),HSIL에서 51명 중 47명(92.2%),자궁경부암에서 62명 중 59명(95.1%)에서 HPV 양성이었으며, 그 중 편평세포암환자 55명 중 53명(96.4%)에서, 선암 환자 7명 중 6명(85.7%)에서 HPV 양성 소견이 관찰되었다 HPV-16형은 정상군에서 7.9% (23/290), LSIL에서 24.2% (16/68), HSIL에서 33.3% (17/51), 자궁경부암에서 75.8% (47/62) 검출되었으며, 자궁경부 병소의 중증도가 진행됨에 따라 HPV-16형의 발견 빈도가 유의하게 증가됨을 관찰할 수 있었다(β<0.01). 그 외 HPV-18, -58형들은 자궁경부암 환자와 HSIL에서 높게 검출되었으며, HPV-18, -51, -52, -58, -68형들은 LSIL에서 높게 검출되었다(β<0.05). 여러 종류의 HPV들이 검출되는 복합 감염의 경우는 정상군에서 19명(37.3%), LSIL에서 21명(42.0%), HSIL에서 13명(27.7%), 자궁경부암에서 17명(28.8%)에서 바이 러스가 발견되 었다한국 여성의 자궁경부 정상 세포진에서 HPV 감염, 자궁경부 상피이형증을 거쳐서 자궁경부암으로 진행되는 상태에 따라 HPV 감염률의 차이와 HPV 유전자형의 분포도 및 복합 감염 여부를 파악할 수 있었다. 자궁경부병소를 가진 여성에서 HPV를 탐색한 본 연구 결과로서 HPV감염과 자궁경부 상피 이형증의 예후를 결정할 수있는 분자 생물학적 지표로 사용하거나 자궁경부 상피내 암과 자궁경부암을 조기 진단하기 위한 보조적 인 방법으로 가능성을 고려할 수 있을 것으로 생각되며, 자궁경부암의 예방을 위하여 향후 개발될 HPV 백신을 사용하기위한 한국 여성에서의 기초 자료로서 가치가 있을 것으로 사료된다. Objective : It has been widely acknowledged that human papillomavirus (HPV) is the most important cause of cervical carcinoma, and the HPV DNA test has been proved as an effective adjuvant to Pap smear. As the development of HPV vaccine is now at the planning stage, the role of HPV genotype profile has become very important in early diagnosis, management and prevention of cervical neoplasia. Methods : Analysis was made on the HPV genotype profile of a number of the inpatients and outpatients who visited at the Department of Gynecology, Kangnam St. Mary’s Hospital between November, 2001 and May, 2002, based on the Pap smear test and tissue pathology. The age range of the patients covered in this study was between 21 years and 91 years and the average age of the patients was 43.4 years. Based on the results of the Pap smear and tissue pathology, the patients were broken down into the following 4 groups; the women with normal cytology (n=290), the patients with LSIL (low-grade squamous intraepithelial lesion); koilocytosis and mild dysplasia (n= 68), the patients with HSIL (highgrade squamous intraepithelial lesion); moderate and severe dysplasia, or carcinoma in situ (n= 51), and the patients with cervical cancer (n=55), respectively and a study was made on the HPV genotype profile of cervix of each of the above 4 groups by utilizing HPV DNA chip. Results : The proportions of the detected HPV-16 in the individual groups were 7.9% (23/290) for women with normal cytology, 24.2% (16/68) for the patients with LSIL, 33.3% (17/51) for HSIL, 75.8% (47/62) for those with cervical cancer, respectively and it was noticed that detection rates increased significantly in accordance with advancing the severity of cervical lesions (P<0.01). Of the rest of the HPV types, HPV -18, -33, -35, -39, -52, -56, -58 were related with cervical cancer (P<0.05); HPV-18, -58 were related with HSIL (P<0.05); HPV -18, -39, -51, -52, -58, -68 were related LSIL (P<0.05). In the case of multiple HPV infections, 19 (37.3%) were detected in women with normal cytology, 21 (42.0%) were detected in patients with LSIL, 13 (27.7%) were detected in HSIL, and 17 (28.8%) were detected in those with cervical cancer, respectively, but there was no statistically significant differences. Conclusion : As a result of this study, HPV genotype profiles and multiple infections by pathological severity can be obtained for the Korean female patients groups ranging from normal cervical cytology through cervical intraepithelial neoplasia to cervical carcinoma. It is expected that the results of this study might be utilized as a molecular biological index to determine prognosis of HPV infection and cervical dysplasia or as an adjunctive diagnostic method for early detection of carcinoma in situ and cervical carcinoma, and that the results can also become very valuable basic data that can be used in developing HPV vaccine for prevention of cervical carcinoma in the near future.