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개 REM134 유선종양세포의 sphere 형성을 통한 암 줄기세포 특성 분석
변정수,이지현,정다운,구나연,조인수,차상호,Byeon, Jeong Su,Lee, Jienny,Jeong, Da-Un,Gu, Na-Yeon,Cho, In-Soo,Cha, Sang-Ho 대한수의학회 2018 大韓獸醫學會誌 Vol.58 No.4
Canine mammary tumors are among the most frequently observed cutaneous tumors in female dogs. Cancer stem cells (CSCs), referred to as tumor-initiating cells, are thought to have properties similar to normal stem cells such as the ability to self-renewal and to differentiate into various cell types. Biological understanding of CSCs and the critical pathways involved in their maintenance are important in research and therapy for mammary tumors. We conducted the present study on sphere formation from REM134 cells by using methylcellulose to produce tumorspheres on a large scale and compared the specific markers of the spheres-formed and plating-cultured REM134 cells. The results revealed that the tumorspheres cultured in methylcellulose had higher seeding density and improved morphology compared to those produced in normal sphere formation medium. Expression levels of stemness markers and CSC-related markers were higher in tumorsphere-forming cells than in plating-cultured cells. Subsequently, we transplanted the tumorsphere-forming and plating-cultured cells into female nude mice to examine their tumorigenic potential. Tumor volume increased rapidly in mice transplanted with tumorsphere-derived cells compared to plating-cultured cells. We observed a novel sphere-forming condition for REM134 cells and showed that REM134 cell tumorspheres can exhibit improved CSC properties.
변정수,이은홍,강정용,류명현,홍순근 한국연초학회 1993 한국연초학회지 Vol.15 No.1
Ridger and vinyl mulcher for 8-10 PS power tiller which were distributed at the rate of one out of 2.3 farm households in Korea, was developed to ease the labor shortage of tobacco production. Devices wheel shaft extension by 30cm at both sides improved the stability of straight drive and enabled to save required labor hours by 50% for ridging at sloping field. Screw type blades were attached on center drive rotavator shaft, gear set was deviled to reverse the rotavator, and it was good at need to adjust the width and height for ridge. As the results, required labor hours for ridging and vinyl mulching could be saved by 90% as compared to conventional manual method after cattle plowing, and by 50% as compared to conventional power tiller method.
변정수,이제니,이경빈,김희량,구나연,조인수,차상호,김도형 한국예방수의학회 2016 예방수의학회지 Vol.40 No.1
Mesenchymal stem cells (MSCs) are multipotent stem cells, which can be induced to differentiate into several cells. MSCs are also reported to possess immunomodulatory properties through secretion of inflammatory cytokines and generation of regulatory T (Treg) cells. Treg cells play an important role in allergic disorders, including atopic dermatitis. We examined the immunomodulatory effects of canine adipose tissue derived-MSCs (cAD-MSCs) in 3 groups: Group 1, untreated normal dog; Group 2, dogs with Dermatophagoides farinae ointment-induced atopic dermatitis; and Group 3, dogs with atopic dermatitis. Canine peripheral blood mononuclear cells (PBMCs) were collected from each group and co-cultured with cAD-MSCs. After co-culturing, PBMCs were separated and the expression of Treg cells was examined by flow cytometry. According to the results, the populations of Treg cells were increased in 3 ex vivo experimental groups, co-cultured with cAD-MSCs. These results would be important for the application of MSCs in clinical trials.
Isolation and characterization of deer-derived mesenchymal stem cells
변정수,구나연,정다운,김찬란,조인수,송재영,현방훈,이지현 한국예방수의학회 2019 예방수의학회지 Vol.43 No.3
Mesenchymal stem cells (MSCs) are capable of differentiating into mesenchymal tissue such as bone, cartilage, muscle, and adipose, and have been isolated and characterized from various species. Deer adipose tissue-derived MSCs (dAD-MSCs) have not been studied and deer bone marrow-derived MSCs (dBM-MSCs) have not been fully characterized. In this study, we firstly isolated MSCs from deer tissues and then compared characteristics of dAD-MSCs and dBM-MSCs. dAD-MSCs and dBM-MSCs exhibited significant increase in proliferation under low-glucose DMEM culture condition during 20 and 10 passages consecutive passages, respectively. Both cells expressed cell surface markers such as CD73, CD90, and CD105, but did not express CD34 and CD45. Two types of cells expressed stemness markers (Oct4, Sox2, and Nanog) and exhibited differentiation potential into mesodermal lineages. Both cells exhibited osteogenic and chondrogenic differentiation potential, but poor adipogenic differentiation potential. Specifically, dAD-MSCs have a greater capacity for chondrogenic differentiation potential compared to dBM-MSCs. Collectively, we successfully isolated dAD-MSCs from deer for the first time. This study suggests that adipose tissue of deer could be used as a source of deer MSCs.