제24회 학술발표회 논문초록

한국연초학회 한국연초학회 1990 한국연초학회지 Vol.12 No.2

담배, 토마토 및 고추에서 분리된 TMV 계통의 생물학적, 물리화학적 및 혈청학적 특성

박은경,이청호,이영기,김영호 한국연초학회 1997 한국연초학회지 Vol.19 No.1

Three strains of W isolated from tobacco, tomato and Pepper plants in Korea were characterized based on biological response, serological relationship, and peptide mapping of the capsid Proteins. The strains designated as TMV-common, TMV-Pepper, and TMV-tomato could be distinguishable by different visual symptoms on 3 varieties of tobacco, one variety of tomato and Pepper for each among 27 plant specieces. Serological relationships were examined by agar gel double diffusion test. Only traceable or weak reaction was observed in the incompatible antigen-antibody combinations. The Pepper strain, however, showed trace in reaction with other two antisera. Peptide maps of the capsid proteins digested by V8 protease or by trypsin were also distinguishable, suggesting differences in composition and/or sequence of the amino acids among the strains.

환경이 조절되는 Chamber 내에서 Environmental Tobacco Smoke의 생성과 감소 현상

이문수,나도영,안기영,이규서 한국연초학회 1996 한국연초학회지 Vol.18 No.2

This paper describes the generation and decay phenomena of gas, vapor and particulate phase components of environmental tobacco smoke in 18 m3 controlled experimental atmosphere chamber. Real time-weighted average concentration ratios of markers were determinated at no ventilation rates and sampling durations of starting to smoking 45 min. Average concentration of major ETS markers was no significant on the mainstream smoke contents of commercial cigarette and decay ratios were dependent on first order kinetic. RSP/nicotine, solanesol and 3-EP were good predictors of ETS concentration in the public indoor field. The concentration ratio of vapor phase and particulate phase components is highly variable to assessment of indoor air quality with ETS. Key words : ETS, chamber study, ETS markers.

담배의 수분 흡착 특성과 흡착 등온식의 모델에 관한 연구

오인혁,김기환,정경락,O, In-Hyeok,Kim, Gi-Hwan,Jeong, Gyeong-Rak 한국연초학회 1996 한국연초학회지 Vol.18 No.2

In this study, to obtain the basic data for the optimum moisture control system, moisture adsorption characteristics, adsorption isotherms models for water and surface physical characteristics of burley and flue-cured tobacco were investigated. By the hypothesis' the phenomenon of moisture adsorption of tobacco is the same as the first order reaction, the wetting constant (k) and equilibrium moisture content were obtained. And activation energy, frequency factors were also calculated by applying its data to Arrhenius equation. The Kamei's empirical formula of moisture adsorption isotherms showed the best agreement with the experimental data and its correlation coefficient (r) was 0.997. It can be seen that specific surface area of burley is 157 m2/g, that of flue-cured is 152 m2/g, -△H1 of adsorbed monolayer is 45,972 J/mol, 45,486 J/mol, respectively, and the condensation heat (40,595 J/mol) being caught in adsorbed multilayer is less than that of monolayer.

$CO_2$를 이용하여 팽화된 각초배합율이 제조담배 물성에 미치는 영향

이영택,조시형,김성한,백신,임광수,김영호,신창호,Lee, Young-Tack,Jo, Si-Hyung,Kim, Sung-Han,Baek, Shin,Rhim, Kwang-Soo,Kim, Young-Ho,Shin, Chang-Ho 한국연초학회 1996 한국연초학회지 Vol.18 No.2

The experiment was carried out to study the effect of dry ice expanded tobacco (DIET) blending ratio on the physical properties of cigarettes. Vnencapsulated pressure drop (UPD) of the cigarette decreased from 85 mmWG to 78 mWG when the DIET blending ratio increased from 0% to 60%, but it increased in the range of over 60% blending ratio. But the overall UPD/EPD ratio of these cigarettes showed a decreasing tendency. Cigarette ventilation rate increased from 55% to 66% with the increased blending ratio of expanded tobacco and loose ends also showed the same tendency. The higher the blending ratio of expanded tobacco, the faster became the static burning time from 7' 34" to 5' 02". However, the cigarette hardness was almost not affected by the blending ratio.ing ratio.

담배의 엽 발달 및 노화과정 중 이화학성 변화

이상각,장병화,석영선,배길관,노재영 한국연초학회 1996 한국연초학회지 Vol.18 No.2

This experiment was conducted to obtain the basic information on the morphological and physiological changes in tobacco leaf during the growth period by measuring the changes of chlorophyll, sugar, lipid and mineral contents in tobacco plant. Leaf length and width have been fully developed at 25 days after leaf emergence. Dry weight was rapidly increased between 10 and 15 days after leaf emergence and reached the highest at 30 days. Crude lipid content, palmitic acid, and the major saturated fatty acid were increased with progressing senescence, while unsaturated fatty acid including linolenic acid was decreased as the senescence was advanced. The total nitrogen content showed the highest value at IS days after leaf emergence. On the other hand, the total sugar content showed the highest value at 45 days after leaf emergence and glucose, fructose and sucrose were decreased with leaf development and increased at the end of senescence. The content of chlorophyll showed the highest value at 15 days after leaf emergence and began to decrease at 30 days after leaf emergence. The contents of p, Cu, Zn, and Fe in tobacco leaves were decreased by the end of senescence after leaf emergence but those of Ca, Mg, and Mn in leaves were increased. Key words : Nicotiana tabacum chlorophyll, fatty acid, senescence.

감자 바이러스 Y 복제유전자 cDNA로 형질전환된 황색종 담배의 저항성 특성

박은경,백경희,유진삼,조혜선,강신웅,김영호 한국연초학회 1997 한국연초학회지 Vol.19 No.1

A flue-cured tobacco variety (Nicotiana tabacum cv. Wisconsin) was used for Plant transformation with the complementary DNA (cDNA) of potato virus Y-necrosis strain (PVY-VN) replicase gone (Nb) which was synthesized through reverse-transcription Primed with oligo(dT) and Polymerization using RNase H-digested template. The cDNA was cloned into Plant expression vector Plasmid (PMBP2), and introduced into tobacco plants by co-culturing tobacco leaf disks with Agrobacterium tumefaciens LBA4404 containing the plasmid before Plant regeneration. Eight Plants, in which the inserted cDNA fragment was detected by Polymerase chain reaction (PCR), out of 70 putative transformants inserted with sense-oriented Mb cDNA showed no symptom at 3 weeks after inoculation, while the other 62 plants, and all plants with vector gone only and antisense-oriented NIb cDNA had susceptible vein-necrosis symptoms. However, only 2 of the 8 resistant plants were highly resistant, which remained symptomless up to 10 weeks after inoculation. Among the first progenies (T1) from self-fertilized seeds of the two resistant transgenic plants, less than 10 % of 71 plants appeared highly resistant (with no symptom), 70% moderately resistant (with mild symptoms on 1 - 2 leaves), and about 20% susceptible (with susceptible symptoms on 3 or more leaves) at 3 weeks after inoculation. These results suggest that the PVY resistance was inherited in the 71 generation. Key words : potato virus Y. viral replicase gene, transgenic tobacco Plants, resistance.

육묘포트 깊이가 담배의 뿌리발달 및 분포에 미치는 영향

이상각,심상인,강병화,이학수,석영선 한국연초학회 1997 한국연초학회지 Vol.19 No.1

The study was carried out to clarify reasonable production of healthy seedling, optimal Pot depth, and appropriate transplanting time, which can be deduced from understanding of seedling quality. Seedling quality results from growth of root and shoot, morphology and distribution of root system under influence of Pot depth during seedling growing period. Stem height, shoot dry weight, leaf area and leaf number were increased in proportion to depth of pots. Growth of shoot and root during seedling growing period showed the most dramatic development between 20th and 25th day after temporary planting. Root number increased as pot depth decrease and total root length and dry weight increased as pot depth increase. In 5cm pot, relative multiplication rate was higher and mean extension rate was lower than other depth of Pot. The limitation of pot volume in which rhizosphere was located enhance the development of roots of second and third order. At 20th days after temporary Planting root distribution was relatively uniform in length and development of adventitious root on stem base was poor as Pot depth decreased.

사료작물 윤작재배에 의한 연초포지의 제염효과

이철환,진정의,한철수,Lee, Chul-Hwan,Jin, Jeong-Eui,Han, Chul-Soo 한국연초학회 1997 한국연초학회지 Vol.19 No.1

The field experiment for field desalinization by precultivation of orchard crops were carried out to evaluate relationship between the varieation of chlorine contents of soil and crop uptake in the upland diverted from paddy field. After harvest of grass crops, soil samples were taken for analysis of chlorine contents of soil layers. Regardless of kinds of grass crops cutivated, contents of soil chlorine were decreased comparing to non-crop plot. Chlorine content in plant harvested at just before the flowering stage was much higher than that of after flowering. Chlorine uptake and dry matter were increased in order of Italian ryegrass, Perennial ryegrass, and Sudan grass. Positive correlations were showed between chlorine uptake and dry matter. The content of soil chlorine decreased by higher yield of dry matter.

버어리종 잎담배 대말림시 수평대걸기에 관한 연구

배성국,김요태,임해건,조천준 한국연초학회 1997 한국연초학회지 Vol.19 No.1

This study was carried out to develop a new stalk curing of the horizontal hanging method that would reduce the harvesting and curing labor, and improve its quality of cured leaf than the conventional stalk curing method in burley tobacco. The horizontal hanging stalk-curing method designed from 1988 was compared with the conventional method. The horizontal method shortened the curing Period for 7 days and reduced to 0.3%, while it occurred 9.4% in the vertical method. In the horizontal method, the quality of cured leaf improved 9%, but the contents of chemical components and physical properties were similar to those of vertical method. The labor of stalk-cut and loading in curing facility reduced 28% by the horizontal method. In harvesting and curing process, the horizontal method resulted 12% of labor saving as compared with the vertical method. Key words : horizontal hanging stalk-curing method, vertical method, stalk-curing.