모기유충에 대한 살충성 Bacillus thuringiensis H9B 균주의 특성

이기희,김광현,김병우 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

One strain of mosquitocidal Bacillus thuringiensis, H9B, was isolated from soil. The biochemical characteristics and flagella antigenicity of the strain H9B is similar to that of B. thuringiensis subsp. darmstadiensis. The delta-endotoxin of the strain H9B coincided with that of B. thuringiensis subsp. darmstadiensis strain 73E10-2 on agarose double immunodiffusion test. The delta-endotoxin of B. thuringiensis subsp. israelensis contains hemolysin fragment (28 kd) on SDS-PAGE when the delta-endotoxin was solubilized in alkali, while that of the strain H9B does not contain 28 kd protein. In the biological activity, the delta-endotoxin of strain H9B was 2 or 3 times stronger than that of the strain 73E10-2; The LC_50 against mosquito larvae (Aedes agypti) of delta-endotoxin from the strain H9B is 70 ng/ml, and that from B. thuringiensis subsp. israelensis and the strain 73E10-2 26 ng/ml, 186 ng/ml respectively.; The hemolytic activity of delta-endotoxin from the strain H9B has twice stronger than that from the strain 73E10-2.

음식물 쓰레기의 퇴비화에 관한 연구

전기일,김형갑,정병윤 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

The purpose of this study were the food wastes composting, this study was carried out in the batch reactor with sawdust as bulking agent. The sample of food wastes for this study were collected fron the restaurant in our University. We obtained the results as follows : 1) During composting process, the highest temperature in the batch reactor was 55.8℃ after 5 days of experiment. 2) During composting process CO_2 and NH_3 were generated, after 6 days of experiment their concentrations were 20% and 640ppm respectively. 3) The volume of sample was reduced to one third in experimental period.

太陰調胃湯이 白鼠의 肥滿症 및 誘導肥滿細胞에 미치는 效果

田炳薰,金敬堯,李基珠 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.1

太陰調胃湯은 四象體質中 太陰人에 적용되는 處方으로 그 구성은 주로 祛濕, 祛痰하는 藥物로 이루어져있다. 韓醫學에서 肥滿의 병리기전은 주로 濕痰이 체내에 전체된 것으로 보고 있는데 太陽調胃湯의 處方구성은 이와같은 體內濕痰을 제거하는데 알맞음을 볼 수가 있다. 이에 太陽調胃湯이 gold thioglucose와 高脂肪食餌로 誘發한 肥滿 마우스에 미치는 效果와 前指肪細胞의 增殖과 分化에 미치는 影響을 실험하고 관찰한 결과 다음과 같은 결과를 볼 수 있다. 1. 太陽調胃湯은 前脂肪細胞(3T3-L1 세포)의 增殖과 分化를 抑制한다. 2. 細胞內 脂質蓄積을 誘導하는 酵素와 中性脂質의 細胞內 蓄積을 抑制하는 效果가 認定된다. 以上의 結果로 보아 太陰調胃湯은 四象體質中 가장 肥濕하기 쉬운 太陽人의 肥滿治療에 有用할 것으로 思料된다. Effects of Taeyeumjoweetang on the obesity of mouse induced by gold thioglucose. It is researched to elucidate the effects of Taeyeumjoweetang on the obesity of mouse induced by gold thioglucose and the diifferentiation and growth of preadipocyte, 3T3-L1 cell. The result were as follows. 1. Taeyeumjoweetang extract improved the blood level of transaminase changed by the obesity of mouse induced by gold thioglucose. 2. Taeyeumjoweetang extract inhibited the increase of liver fat and body fat induced by the obesity of mouse induced by gold thioglucose. 3. Taeyeumjoweetang extract inhibited the increase of body weight induced by the obesity of mouse induced by gold thioglucose. 4. Taeyeumjoweetang extract inhibited the growth of undifferentiate preadipocyte 3T3-L1. 5. Taeyeumjoweetang extract showed the effects of inhibition on the differentiation of preadipocyte 3T3-L1. The above results suggested that the Taeyumjoweetang extract may be used on the obesity induced by the overgrowth and differentiation of adipocyte, and the accumulation of fat in liver and body.

Benzene 분해 미생물의 분리 및 특성

서현호,정병운,전기일,김형갑 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

Flavobacterium sp. BEN2 was selected and identified out of 64 benzene-degrading bacteria isolated from soils and waste waters by the enrichment culture technique. The optimal temperature, pH, and benzene concentration for the growth of Flavobacterium sp. BEN2 appeared to be 30℃, 7.0 and 2000 ppm, respectively. Cell growth almost revealed a stationary phase at 24 hours after cultivation and benzen was completely degraded during as benzen, benzoic acid, phenol, p-cresol, salicylic acid, catechol, gentistic acid and toluene, but did not grow ob o-cresol, m-cresol, p-xylene and p-toluic acid. With this strain, catechol was degraded through both meta- and ortho- cleavage pathway. Therefore, it is suggested that Flavobacterium sp. BEN2 could be effectively used for the biological treatment of wastewater containing benzene in the presence of some aromatic compounds and organic solvents.

EFFECTS OF LIDOCAINE ON SOMATOSENSORY EVOKED POTENTIALS IN RAT VIBRISSA/BARREL CORTEX

남순현,최병주,김현정,이만기,김영진,이혜숙 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

본 실험은 삼차신경 자극으로 발생되는 체성 감각 유발 전위에 대한 국소마취제의 효과를 관찰하였다. 나트륨 통로차단을 통하여 약리작용을 나타내는 것으로 알려져 있는 리도카인를 뇌 피질에 국소 투여한 후 삼차신경의 체성 감각유발 전위의 강도및 지연시간을 측정하였다. 케타민으로 마취된 흰쥐의 대측성 구레나룻 자극후 뇌의 체성 감각영역으로부터 기록되는 유발전위를 분석한 결과, 리도카인을 뇌 피질에 국소 투여시 유발전위의 강도 및 지연시간의 감소가 나타났으며, 필드 전위의 형태는 이상성(양극성 및 음극성) 혹은 삼상성(양극성, 음극성 및 양극성)의 파형으로 나타났다. 필드 전위의 발생 부위는 뇌 피질의 중대뇌동맥의 상행지 상방영역이었다. 본 실험에서 나타난 초기 전위변동은 피질판 상층에 존재하는 신경세포의 탈분극 과정에 의하여 생성되고 후기 전위 변동은 피질판 상층에 존재하는 신경세포의 탈분극 과정에 의하여 생성되고 후기의 전위 변동은 동일 영역의 하층 신경세포에서 과분극 혹은 재분극이 발생한 결과라고 유추된다. 따라서 삼차신경계의 체성 감각 영역에서는 피질 상층및 하층의 과립성 피라미드 세포의 순차적인 활성화에 의하여 기본적인 신경 회로망이 형성되어 있으며 생리적 자극으로 유발되는 필드 전위는 이러한 신경망를 통하여 발생될 것으로 사료된다.

해마 theta리듬과 pyramidal neuron의 세포내 특성과의 상관관계

권오흥,조진화,남순현,김현정,최병주,김영진,이만기 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.4

Eledctrophysiological phenomena of pyramidal cells in the CAI area of the dorsal hippocampus were recorded from and filled with neurobiotin in anesthetized rats. The electropharmacological properties of membrane as well as the cellular-synaptic generation of rhythmic slow activity (theta)were examined. The intracellular response characteristics of these pyramidal cells were distinctly different from responses of inteneurons. Pyramidal cells had a high resting membrane potential, a low input resistance, and a large amplitude action potential. A afterhyperpolarization was followed a single action potential. Most of pyramidal cells did not display a spontaneous firing. Pyramidal cells displayed weak inward rectification and anodal break excitation. The slope of the frequency-current relation was 53.4 Hz/nA for the first interspike interval and 15.9 Hz/nA for the last intervals, suggesting the presence of spike frequency adaptation. Neurobiotin-filled neurons showed pyramidal morphology. Cells were generally bipolar dendritc processes ramifying in stratum lacunosum-moleculare, radiatum, and oriens. Commissural stimulation discharged pyramidal cells, followed by excitatory and inhibitory postsynaptic potentials (EPSPs and IPSPs). The frequency of theat-related membrane potential oscillation was voltage-independent in pyramidal neurons. At strong depolarization levels (less than 30 mV) pyramidal cells emitted sodium spike oscillation, phase-locked to theta. The observations provide direct evidence that theta-related rhythmic hyperpolarization of principal cells is brought by the rhythmically discharging interneurons. Furthermore, the findings in which interneurons were also paced by rhythmic inhibitory postsynaptic potentials during theta suggest that they were periodically hyperpolarized by their GABAergic septal afferents.

혈관 확장술에 실패한 동정맥루의 상부정맥 폐쇄 및 협착증에 대한 혈관내 스텐트의 치료효과

김병수,김용수,박주현,김영옥,송하헌,윤선애,신미정,양철우,방병기,김기태,고기영 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.6

Although percutaneous angioplasty is effectively used in the treatment of vascular access stenosis in hemodialysis patients, it has low initial success rate and high recurrence rate for proximal vein obstruction or stenosis. We evaluated the effect of endovascular stent placement on the treatment of proximal vein obstruction or stenosis irresponsive to angioplasty in hemodialysis patients. Wallstent was placed in 8 hemodialysis patients with vascular access obstruction or stenosis. All lesions were proximal to a functioning access which had been created one month to 4 years prior to on set of symptoms. Of the total patients, 4 patients had central vein stenosis(2 subclavian, 2 innominate vein stenosis) and they all had a history of subclavian vein catheterization for hemodialysis. Seven patients presented with arm edema, one suffered from needling difficulty. Venography showed complete obstruction in 4 patients and severe stenosis in 4 patients. Angioplasty was attempted before stent placement but failed in all patients. The stent placement initially succeeded in all patients. There were no acute complications such as stent displacement, sepsis, and bleeding. After this intervention, clinical symptoms disappeared and all patients could be immediately treated with hemodialysis via corrected access. The patients were followed for 8.0±4.6 months (3-16 months). During this period, restenosis occurred in 2 patients. Of the 2 patients, one patient was successfully treated with angioplasty. In conclusion, endovascular stent placement seems to be effective on the treatment of proximal vein obstruction or stenosis irresponsive to angioplasty in hemodialysis patients.

Exploiting transcriptomic data for metabolic engineering: toward a systematic strain design

Kim, Minsuk,Park, Beom Gi,Kim, Joonwon,Kim, Jin Young,Kim, Byung-Gee Elsevier 2018 Current opinion in biotechnology Vol.54 No.-

<P>Transcriptomics is now recognized as a primary tool for metabolic engineering as it can be used for identifying new strain designs by diagnosing current states of microbial cells. This review summarizes current application of transcriptomic data for strain design. Along with a few successful examples, limitations of conventionally used differentially expressed gene-based strain design approaches have been discussed, which have been major reasons why transcriptomic data are considerably underutilized. Recently, integrative network-based approaches interpreting transcriptomic data in the context of biological networks were invented to provide complimentary solutions for metabolic engineering by overcoming the limitations of conventional approaches. Here, we highlight recent pioneering studies in which integrative network-based methods have been used for providing novel strain designs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Transcriptomic data can facilitate strain design but yet underutilized. </LI> <LI> Differentially expressed genes have been primary targets for metabolic engineering. </LI> <LI> Integrative network-based analysis of transcriptomic data could provide novel strain design. </LI> <LI> More computational tools are needed to fully exploit transcriptomic data for strain design. </LI> </UL> </P>

MALDI-MS-Based Quantitative Analysis for Ketone Containing Homoserine Lactones in <i>Pseudomonas aeruginosa</i>

Kim, Yoon-Woo,Sung, Changmin,Lee, Seulee,Kim, Kyoung-Jin,Yang, Yung-Hun,Kim, Byung-Gee,Lee, Yoo Kyung,Ryu, Hee Wook,Kim, Yun-Gon American Chemical Society 2015 ANALYTICAL CHEMISTRY - Vol.87 No.2

<P><I>N</I>-Acyl homoserine lactones (AHLs), quorum sensing molecules produced by Gram-negative bacteria, are used as important secondary metabolites for antibacterial drug development and cell-to-cell communication. Although various analytical techniques have been developed for detection and quantitation of AHLs from more complex bacterial culture media, only a few methods have been applied to AHL identification in physiological samples. Here, we developed a highly sensitive and reliable MALDI-based 3-oxo AHL quantitation method by employing Girard’s reagent T (GT) to produce a permanent cationic charge state [M]<SUP>+</SUP> at the ketone group of AHLs. After extracting AHLs from the supernatant of bacterial cultures using ethyl acetate, the extracts were subsequently derivatized with GT without any additional purification or desalting steps. The chemical derivatization of 3-oxo AHLs dramatically enhanced sensitivity (up to 60 000 times) by lowering the limit of detection (LOD, ∼0.5 fmol)/limit of quantitation (LOQ, ∼2.5 fmol). Additionally, the GT-derivatized 3-oxo AHLs allowed more accurate quantitative analysis from the <I>Pseudomonas aeruginosa</I> PAO1 culture supernatants. This method may be applied for developing high-throughput and sensitive detection methods of quorum sensing signal molecules in biofilm-related clinical applications such as virulence factor characterization and antibacterial drug development.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2015/ancham.2015.87.issue-2/ac5039362/production/images/medium/ac-2014-039362_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac5039362'>ACS Electronic Supporting Info</A></P>

Identification and Characterization of the Rhizobium sp. Strain GIN611 Glycoside Oxidoreductase Resulting in the Deglycosylation of Ginsenosides

Kim, Eun-Mi,Kim, Juhan,Seo, Joo-Hyun,Park, Jun-Seong,Kim, Duck-Hee,Kim, Byung-Gee American Society for Microbiology 2012 Applied and environmental microbiology Vol.78 No.1

<B>ABSTRACT</B><P>Using enrichment culture,Rhizobiumsp. strain GIN611 was isolated as having activity for deglycosylation of a ginsenoside, compound K (CK). The purified heterodimeric protein complex fromRhizobiumsp. GIN611 consisted of two subunits with molecular masses of 63.5 kDa and 17.5 kDa. In the genome, the coding sequence for the small subunit was located right after the sequence for the large subunit, with one nucleotide overlapping. The large subunit showed CK oxidation activity, and the deglycosylation of compound K was performed via oxidation of ginsenoside glucose by glycoside oxidoreductase. Coexpression of the small subunit helped soluble expression of the large subunit in recombinantEscherichia coli. The purified large subunit also showed oxidation activity against other ginsenoside compounds, such as Rb1, Rb2, Rb3, Rc, F2, CK, Rh2, Re, F1, and the isoflavone daidzin, but at a much lower rate. When oxidized CK was extracted and incubated in phosphate buffer with or without enzyme, (<I>S</I>)-protopanaxadiol [PPD(S)] was detected in both cases, which suggests that deglycosylation of oxidized glucose is spontaneous.</P>