桃紅四物湯이 L1210細胞가 移殖된 마우스의 免疫系에 미치는 效果

趙鈴林,鄭鉉雨 대한동의병리학회 1999 동의생리병리학회지 Vol.13 No.1

桃紅四物湯은 醫宗金鑑에 最初로 收錄된 處方으로 四物湯에 桃仁·紅花를 加味한 處方이다. 本 方은 月經不調나 經前腹痛등 瘀血로 인한 부인의 각종 月經異常에 사용되는 處方으로써 지금까지 대부분 血流障碍疾患에 대한 硏究를 진행하여 왔다. 腫瘍은 현대인의 사망률 중 가장 높은 비율을 차지하고 있는 疾患중의 하나로 東醫學에서는 이를 치료하기 위하여 正氣補養·補血 및 破積·活血·解鬱·行氣등의 治法들을 兼用하고 있으며, 西醫學에서는 化學療法·放射線療法·手術療法·遺傳子療法·免疫療法등을 사용하고 있으나 그에 대한 副作用등 많은 문제점들을 해결하지 못하고 있는 실정이다. 그리하여 天然藥物로 구성된 韓藥材가 이러한 副作用을 解消시켜 주고, 또한 "正邪鬪爭"관점에서 '正氣'가 보강되면 免疫力도 增强됨으로써 癌細胞에 대한 抗病能力이 뛰어날 것으로 思料되기 때문에 瘀血을 치료하는 동시에 補血할 수 있는 桃紅四物湯을 癌細胞가 移植한 마우스에 경구투여한 다음 抗癌作用 및 免疫機能 活性化를 살펴보기로 하였다. 實驗方法으로 급성백혈병세포주인 L1210 cell line에 대한 citotoxicity에 미치는 桃紅四物湯의 效果를 MTT assay를 통해 살펴보고, 이와 동시에 in vivo상에서 L1210細胞柱를 2×106cells/mouse씩 腹腔에 주입한 후 桃紅四物湯을 1일 1회씩 7일간 경구투여한 후 癌細胞의 增殖과 T lymphocytes, B lymphocytes의 活性度, 그리고 腹腔 macrophages에서 생성되는 NO의 量을 측정한 結果 in vitro상으로 L1210癌細胞柱의 增殖率은 對照群보다는 감소하였지만 臨床的인 有意性은 認定되지 않았지만 in vivo상에서는 對照群에 비하여 약 20(%)정도 L1210細胞의 증식을 억제하였다. 또한 thymocytes의 경우는 正常群과 비슷한 增殖을 나타내었고, 腹腔 macrophage에서 생성되는 NO의 量은 正常群보다도 탁월한 NO의 量을 촉진시켰으며, splenocytes의 增殖은 對照群보다는 增殖이 活性化되었지만 臨床的인 有意性은 인정하기 어려웠다. 이와같은 結果들을 볼 때 補血을 시켜주면서도 瘀血을 치료하는 扶正祛邪의 代表方劑인 桃紅四物湯이 NO에 의한 抗癌效果는 물론 免疫系의 活性化(T cell중 TH cell)에 중요한 역할을 하는 것으로 思料된다. The purpose of this study was to investigate effect of DohongSamulTang(DST) on anti-tumor, proliferation of immunocytes and nitric oxide(NO) production from peritoneal macrophages in L1210 cells-transplanted mice. This study estimated the proliferation of L1210 cells in vitro and in vivo. and estimated the proliferation of thymocytes and splenocytes in L1210 cell-transplanted mice, and the NO production from peritoneal macrophages and body weight in L1210 cells-transplanted mice. The results were as follow ; 1. DST didn't inhibite the proliferation of L1210 cells line in vitro. 2. DST inhibited significantly the proliferation of L1210 cells in L1210 cells-transplanted mice. 3. DST was accelerate significantly the proliferation of thymocytes in L1210 cells-transplanted mice. 4. DST was accelerate the proliferation of splenocytes in L1210 cells-transplanted mice. 5. DST incresed the NO production from peritoneal macrophages in L1210 cells-transplanted mice. 6. DST increased the body weight as comparing with control group in L1210 cells-transplanted mice.

天南星이 생쥐에 移植된 L1210 細胞의 增殖에 미치는 影響

全熏 대한본초학회 2000 大韓本草學會誌 Vol.15 No.2

We have previously observed that the proliferation of L1210 cells was inhibited by the administration of Arisaematis Rhizoma water extract (AE). In this present study, the mechanism of the inhibitory action on the proliferation of L1210 cells was examined. AE decreased the proliferation of L1210 cells and enhanced DNA fragmentation of L1210 cells in vivo system. DNA fragmentation of L1210 cells was enhanced by co-culture of peritoneal macrophages obtained from AE-administered mice in vitro. AE increased nitric oxide production from peritoneal macrophages of L1210-transplanted mice. In addition, AE enhanced the population of CD4^+CD8 cells. These results suggest that the inhibitory action of AE on the proliferation of L1210 cells is partly caused by an induction of apoptosis via a production of nitric oxide in macrophages activated by Th cells.

삼릉전이 생쥐에 이식된 L1210 세포의 증식에 미치는 영향

전용근,임재윤,송정모,은재순,Jeon Yong Keun,Leem Jae Yoon,Song Jung Mo,Eun Jae Soon 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

We studied effects of Samreungjeon water extract (SE) on the proliferation of transplanted-L1210 cells to mice. Samreungjeon is composed of Scirpi Tuber, Zedoariae Rhizoma, Aurantii immaturi Pericarpium, Pinelliae Tuber and Hordei Fructus Germinatus. When SE (500 mg/kg) was administered orally once a day for 7 days after transplantation of L1210 cells to mice, the proliferation of transplanted-L1210 cells was decreased and DNA fragmentation of transplanted-L1210 cells was induced. Also, DNA fragmentation of L1210 cells was enhanced by co-culture with the peritoneal macrophages obtained from SE-administered mice and was partly inhibited by L-NMMA in vitro. SE enhanced the production of nitric oxide from murine peritoneal macrophages in vitro and in vivo. These results suggest that SE partly induces apoptosis of transplanted-L1210 cells via production of nitric oxide from macrophages.

자근에 의한 백혈병 세포주인 L1210 세포의 세포사 유도

원경숙,김대근,오찬호,소준노,은재순 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

Cellular death by apoptosis is an active process, depending on gene transcription and protein synthesis. It was reported that nitric oxide can induce apoptosis in several cancer cell-lines. We studied effects of Lithospermum erythrorhizon Siebold et Zuccarinii (Boraginaceae) Radix water extract (LE) on induction of transplanted-L1210 cells apoptosis in mice. When LE (500 mg/kg) was administered orally once a day for 7 days after transplantation of L1210 cells to mice, DNA fragmentation of transplanted-L1210 cells was induced and mitochondrial transmembrance potential of those cells was reduced. Also, DNA fragmentation of L1210 cells was enhanced by co-culture with the peritoneal macrophages obtained from LE-administered mice and was partly inhibited by L-NMMA in vitro. LE enhanced the production of nitric oxide from peritoneal macrophages. These results suggest that administration of LE partly induces apoptosis of transplanted-L1210 cells in the early stage via stimulation of nitric oxide production from macrophages.

지모(知母) 추출물이 L-1210 및 S-180 암세포주 성장 억제에 미치는 영향

임치혜,초재승,김효수,권승만,김신,김일환,박혜선,Yim, Chi-Hye,Cho, Jae-Seung,Kim, Hyo-Soo,Kwon, Seung-Man,Kim, Shin,Kim, Il-Hwan,Park, Hye-Sun 사상체질의학회 2007 사상체질의학회지 Vol.19 No.2

1. Objective This study was aimed to screen the potential antitumor activity of one kinds of Korean medicinal herb extracts against cancer cell lines and to evaluate the growth inhibition effect of L-1210 and S-180 cancer cell lines. 2. Methods It confirmed Anemarrhena asphodeloides extracts to screen the potential antitumor activity. Then, it was extracted with 4 kinds of solvents ; hexane, ethyl acetate, butanol and $H_2O$, and the Growth inhibition effect of these extracts were determined against cancer cell and normal cell. The results were as follows : The IC50(50% inhibitory concentration) values of Anemarrhena asphodeloides extracts were shown to be $253{\mu}g/ml$ against L-1210 cell lines. The IC50 values of ethyl acetate extracts were shown to be $915{\mu}g/ml$ against L-1210 cell lines. The IC50 values of butanol extracts were shown to be $52.3{\mu}g/ml$, $485{\mu}g/ml$ against L-1210, S-180 cell lines, respectively. The butanol extracts were more selectively effective than other extracts to cancer cell lines. 3. Conclusion From these data, it could be concluded that the Anemarrhena asphodeloides extracts to the Growth inhibition effect of L-1210 and S-180 cancer cell lines.

어성초 추출물의 ICR생쥐와 L1210 세포에 대한 항암작용 및 SOD, GPx 효소활성변화

하혜경,정대영,박시원 대한약학회 2004 약학회지 Vol.48 No.4

The present investigation was undertaken to examine the anticancer activity of the methanol extract from Houttuynia cordata on ICR mouse with induced abdominal cancer and L1210 cancer cells. When the methanol extract of Houttuynia cordata (10∼200 $\mu\textrm{g}$/$m\ell$) was administered orally to ICR mouse with abdominal cancer, 47.8% of the best life prolonging effect was obtained. In case of cytotoxicity study (inhibition of cell proliferation) of Houttuynia cordata extract against L1210 cells, $IC_{50}$/ was found to be 62.8 $\mu\textrm{g}$/$m\ell$. In contrast to such considerable toxicity against cancer cell line, the toxicity demonstrated by the identical extract against normal lymphocytes was very meagre as shown to be < 5% compared with 86.5% in case of L1210 cells at the same condition. To get an insight into the reaction mechanism undelying the anticancer activity, $O_2$ion quantity and antioxidant enzyme activities such as superoxide dismiutase (SOD) and glutathione peroxidase (GPx) of L1210 cells in the presence of Houttuynia cordata extract were measured. The increased values of SOD and GPx enzyme activities in addition to the augmented generation of $O_2$ ion in L1210 cells implied that the reactive oxygen species induding $O_2$ion which were presumably induced by Houttuynia cordata extract might have participated in the process of L1210 cells cytotoxicity.

시호추출물의 ICR 발암생쥐의 생존율 및 J774A.1 세포와 L1210 세포의 증식에 미치는 영향

하혜경,정대영,박시원 한국생약학회 2004 생약학회지 Vol.35 No.4

The current investigation was carried out to find out the anticancer activity of the methanol extract from Bupleurum falcatum against cancered ICR mouse and cancer cell lines such as J774A.1 and L1210 cells. Extract of Buplerum falcatum displayed the considerable augmentation(134%) of the survival of ICR mouse bearing Sarcoma 180 cancer. In addition, the cytotoxic effects of methanol extract of Buplerum falcatum against J774A.1 cells and L1210 cells were found to show IC50 values of 57.3 μg/ml and 54.6 μg/ml, respectively. In contrast to such cytotoxicity against cancer cells, the extract exerted only meagre toxicity against normal lymphocytes. The increased generation of O2 − and the considerably increased activities of superoxide dismutase(SOD) and glutathione peroxidase(GPx) of both J774A.1 cells and L1210 cells in the presence of Buplerum falcatum extract implied that the observed cytotoxicities may have resulted from the detrimental effect of reactive oxygen species(ROS) evoked by Buplerum falcatum extract on the cancer cells.

Studies on the mechanism of cytotoxicities of polyacetylenes against L1210 cell

Kim, Young-Sook,Jim, Seung-Ha,Kim, Shin-Il,Hahn, Dug-Ryong The Pharmaceutical Society of Korea 1989 Archives of Pharmacal Research Vol.12 No.3

This study was performed to investigate the mechanism of in vitro cytotosic actions of polyacetylenes which are panaxydol, panaxynol and panaxytriol isolated from Panax ginseng C. A. Meyer. DNA synthesis of L1210 cells was significantly inhibited with dose dependent pattern when L1210 cells were treated for 1 hour with over 5 .mu.g/ml of polyacetylenes. Panaxydol which had the most potent cytotoxicity among three polyacetylenes showed also the strongest inhibitory effect on DNA synthesis. Intracellular cyclic AMP levels of L1210 cells treated with 2.5 $\mu$g/ml of panaxydol or panaxytriol were significantly elevated on the incubation duration. The elevation of cyclic AMP levels by panaxytriol was higher than that by panaxydol, but no significant increase in cyclic AMP by panaxynol was observed. All three polyacetylenes had no effect on glycolysis of L1210 cells. Electron microscopic observations revealed that polyacetylenes caused damage to plasma membranes of L1210 cells in proportion to their cytotoxicities at each $ED_{50}$ value (panaxydol > panaxynol> panaxytriol). These results suggest that cytotoxicities of polyacetylenes against L1210 cells might be mediated by elevated cyclic AMP level, even though the relationship among their cytotoxicities, inhibitory effect on DNA synthesis and ability to elevation of cyclic AMP level are not fully agreed, and might be also related to membrane damage.

L1210 및 HL60 Cell에 대한 연교의 세포독성 성분

이준성(Jun Sung Lee),민병선(Byung Sun Min),배기환(Ki Hwan Bae) 대한약학회 1996 약학회지 Vol.40 No.4

Forsythiae Fructus was studied on cytotoxic activities for the purpose of finding out active consituents against L1210 and HL60 cells. To isolate the active ones, the methanolic extract was partitioned into water insoluble and water soluble fractions. Furthermore, the water soluble fraction was fractionated into four parts, n-hexane, benzene, ethylacetate and water fractions. Among these, the water insoluble fraction showed the most potent cytotoxic activities on L1210 and HL60 cells in vitro. The water insoluble fraction was applied to silica gel column chromatography and divided into 5 fractions(fr. 1-5). The active constituents I and II were isolated from fr.2 and 3, respectively, by repeated silica gel column chromatography and recrystallization. The constituents were identified as 3beta-acetylbetulinic acid and betulinic acid by means of physicochemical data. The ED50 values of 3beta-acetylbetulinic acid and betulinic acid were 9.10 and 16.43mcg/ml against L1210 cells and 2.72 and 2.41mcg/ml against HL60 cells, respectively.

L1210 및 HL60 Cell에 대한 연교의 세포독성 성분

이준성,민병선,배기환 충남대학교 약학대학 의약품개발연구소 1996 藥學論文集 Vol.12 No.-

Forsythiae Fructus was studied on cytotoxic activities for the purpose of finding out active constituents against L1210 and HL60 cells. To isolate the active ones, the methanolic extract was partitioned into water insoluble and water soluble fractions. Furthermore, the water soluble fraction was fractionated into four parts, n-hexane, benzene, ethylacetate and water fractions. Among these, the water insoluble fraction showed the most potent cytotoxic activities on L1210 and HL60 cells in vitro. The water insoluble fraction was applied to silica gel column chromatography and divided into 5 fractions (fr. 1-5). The active constituents Ⅰ and Ⅱ were isolated from fr. 2 and 3. respectively, by repeated sillica gel column chromatography and recrystallization. The constituents were identified as 3β-a-cetylbetulinic acid and betulinic acid by means of physicochemical data. The ED_50 values of 3β-a-cetylbetulinic acid and betulinic acid were 9.10 and 16.43 ㎍/㎖ against L1210 cells and 2.72 and 2.41 ㎍/㎖ against HL60 cells, respectively.