열 안정성 염 제거장치를 고려한 아민 재생 공정 최적화 전략

이제성 ( Jesung Lee ),임종훈 ( Jonghun Lim ),조형태 ( Hyungtae Cho ),김정환 ( Junghwan Kim ) 한국공업화학회 2020 공업화학 Vol.31 No.5

본 연구에서는 열 안정성 염 제거장치가 추가된 아민 재생 공정을 모사하고, 폐수 량, 열 안정성 염 제거 량, MDEA(methyl diethanolamine) 손실량을 고려한 최적 운전조건 도출 전략을 제시하였다. 산성 가스를 흡수 및 탈거하는 아민재생공정에서 열 안정성 염은 공정 장비 및 아민 용액의 흡수 효율을 저해한다. 열 안정성 염 제거 방법 중 하나인 이온교환수지법은 NaOH와 같은 강 염기성 용액을 사용하여 중화반응을 통해 염을 제거시키는 방법이다. 공정 모델링 과정에서 산성 가스의 탈거 과정은 Radfrac 모델을 사용했고, 반응의 평형상수는 Gibbs 자유에너지를 사용하여 계산하였다. 탈거된 아민 용액의 일부는 열 안정성 염 제거 장치로 들어가게 되고, 제거 장치는 중화반응을 이용한 Rstoic 모델을 사용하였다. 실제 운전데이터와 시뮬레이션 결과를 비교하여 검증하였고, 제거 장치로 들어가는 질량 유량을 조절하여 사례연구를 하고 최적 운전 조건을 제시하였다. In this study, we simulated an amine regeneration process with heat-stable salts removal unit. We derived the optimal operating conditions considering the flow rate of waste, the removal rate of heat-stable salts, and the loss rate of MDEA (methyl diethanolamine). In the amine regeneration process that absorbs and removes acid gas, heat-stable salt impairs the absorption efficiency of process equipment and amine solution. An ion exchange resin method is to remove heat-stable salts through neutralization by using a strong base solution such as NaOH. The acid gas removal process was established using the Radfrac model, and the equilibrium constant of the reaction was calculated using Gibbs free energy. The removed amine solution is separated and flows to the heat-stable salts remover which is modeled by using the Rstoic model with neutralization reaction. Actual operation data and simulation results were compared and verified, and also a case study was conducted by adjusting the inflow mass of removal unit followed by suggesting optimal conditions.

Effects of Heat-treated Bovine Lactoferrin on the Growth of Lactococcus lactis subsp. cremoris JCM 20076

( Woan-sub Kim ) 한국유가공학회(구 한국유가공기술과학회) 2019 Journal of Dairy Science and Biotechnology (JMSB) Vol.37 No.2

This study investigated the effects of heat-treated and non-heat-treated bovine lactoferrin on the growth of Lactococcus lactis subsp. cremoris JCM 20076. The addition of heattreated and non-heat-treated bovine lactoferrin in adjusted MRS medium stimulated the growth of Lc. cremoris JCM 20076. Heat-treated bovine lactoferrin had a greater impact on the growth of Lc. cremoris JCM 20076 compared to that with non-heat-treated bovine lactoferrin. Bovine lactoferrin heated at 65°C for 30 min stimulated the growth of the bacteria more than that heated at 80°C for 5 min. Furthermore, the growth of Lc. cremoris JCM 20076 increased substantially with heat-treated bovine lactoferrin at a concentration of 1 mg/mL.

Effects of Heat-treated Bovine Lactoferrin on the Growth of Lactococcus lactis subsp. cremoris JCM 20076

Kim, Woan-Sub Korean Society of Dairy Science and Biotechnology 2019 Journal of Dairy Science and Biotechnology (JMSB) Vol.37 No.2

This study investigated the effects of heat-treated and non-heat-treated bovine lactoferrin on the growth of Lactococcus lactis subsp. cremoris JCM 20076. The addition of heat-treated and non-heat-treated bovine lactoferrin in adjusted MRS medium stimulated the growth of Lc. cremoris JCM 20076. Heat-treated bovine lactoferrin had a greater impact on the growth of Lc. cremoris JCM 20076 compared to that with non-heat-treated bovine lactoferrin. Bovine lactoferrin heated at $65^{\circ}C$ for 30 min stimulated the growth of the bacteria more than that heated at $80^{\circ}C$ for 5 min. Furthermore, the growth of Lc. cremoris JCM 20076 increased substantially with heat-treated bovine lactoferrin at a concentration of 1 mg/mL.

소아 설사증에서 분리한 대장균 장독소의 병원적 역할

안병수,김경희,한왕수,서인수,Ahn, Byung-Soo,Kim, Kyung-Hee,Han, Wang-Soo,Suh, Inn-Soo 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.2

The incidnce of enterotoxigenic Esherichia coli(ETEC) was investigated in E. coli strains isolated from Korean infants less than two years old. Over a period of 12 months, ETEC strains have been isolated from 45(45.0%) of 100 children with acute diarrhea and from 9(20.5%) of 44 children without diarrhea. In the group with diarrhea, 41(41.0%) strains produced heat-stable toxin, 3(3.1%) produced heat-labile toxin, and 1(1.0%) produced both heat-stable and heat-labile toxins. In the control group, 7(15.9%) released heat-stable toxin, 2(4.5%) released heat-labile toxin and none released both. A statistical association of strains releasing heat-stable toxin was significant(P<0.025).

Heat-Stable Antifungal Factor and Expression Patterns of Protease by Chitinase-Producing Bacterium, Pedobacter sp. PR-M6

송용수,정우진 한국키틴키토산학회 2022 한국키틴키토산학회지 Vol.27 No.1

Abstract This study was conducted to investigate the protease expression patterns of the novel chitinaseproducing bacterium Pedobacter sp. PR-M6. Protease were detected on SDS-PAGE gel as condition of 4o C, 25o C and 37o C to activate of enzymes obtained from incubation of LB, NB and 1% skim milk medium at 4o C, 20o C and 30o C after 21 days of incubation, respectively. Pedobacter sp. PR-M6 displayed proteolytic activity on SDS-PAGE gels containing 0.1% (w/v) gelatin, and protease isozymes of PR-M6 were expressed as two major bands (P7 and P8) and seven minor bands (P1, P2, P3, P4, P5, P6, and P9). Protease isozymes of PR-M6 were inhibited at 4o C and 25o C. The two protease isozymes P5 and P6 were newly expressed in the LB culture medium at 20o C. To investigate the properties of a heat-stable antifungal factor (HSAF), PR-M6 stain was cultured to obtain the products of biosynthesis for incubation in LB medium. HSAF exhibited active biosynthesis at 20o C for incubation in LB medium. Crude HASF obtained from LB20 medium showed hyphal growth inhibition of Rhizoctonia solani. Inhibition rate of R. solani growth was as high as 67% at 24 h and 47.8% at 48 h after incubation with 1,000 ppm of crude HSAF. Trypan blue stained the inside of the edge for destructed R. solani cell walls after crude HSAF treatment. These results indicated that HSAF and protease from Pedobacter sp. PR-M6 could be used as biocontrol agents against phytopathogens in agriculture This study was conducted to investigate the protease expression patterns of the novel chitinaseproducing bacterium Pedobacter sp. PR-M6. Protease were detected on SDS-PAGE gel as condition of 4o C, 25oC and 37oC to activate of enzymes obtained from incubation of LB, NB and 1% skim milk medium at 4oC, 20oC and 30o C after 21 days of incubation, respectively. Pedobacter sp. PR-M6 displayed proteolytic activity on SDS-PAGE gels containing 0.1% (w/v) gelatin, and protease isozymes of PR-M6 were expressed as two major bands (P7 and P8) and seven minor bands (P1, P2, P3, P4, P5, P6, and P9). Protease isozymes of PR-M6 were inhibited at 4o C and 25o C. The two protease isozymes P5 and P6 were newly expressed in the LB culture medium at 20o C. To investigate the properties of a heat-stable antifungal factor (HSAF), PR-M6 stain was cultured to obtain the products of biosynthesis for incubation in LB medium. HSAF exhibited active biosynthesis at 20o C for incubation in LB medium. Crude HASF obtained from LB20 medium showed hyphal growth inhibition of Rhizoctonia solani. Inhibition rate of R. solani growth was as high as 67% at 24 h and 47.8% at 48 h after incubation with 1,000 ppm of crude HSAF. Trypan blue stained the inside of the edge for destructed R. solani cell walls after crude HSAF treatment. These results indicated that HSAF and protease from Pedobacter sp. PR-M6 could be used as biocontrol agents against phytopathogens in agriculture

Effects of Heat-treated Bovine Lactoferrin on the Growth of Lactococcus lactis subsp. cremoris JCM 20076

김완섭 한국낙농식품응용생물학회 2019 한국유가공기술과학회지 Vol.37 No.2

This study investigated the effects of heat-treated and non-heat-treated bovine lactoferrin on the growth of Lactococcus lactis subsp. cremoris JCM 20076. The addition of heattreated and non-heat-treated bovine lactoferrin in adjusted MRS medium stimulated the growth of Lc. cremoris JCM 20076. Heat-treated bovine lactoferrin had a greater impact on the growth of Lc. cremoris JCM 20076 compared to that with non-heat-treated bovine lactoferrin. Bovine lactoferrin heated at 65°C for 30 min stimulated the growth of the bacteria more than that heated at 80°C for 5 min. Furthermore, the growth of Lc. cremoris JCM 20076 increased substantially with heat-treated bovine lactoferrin at a concentration of 1 mg/mL.

계태아연속계대에 의한 내열성 Newcastle disease virus 분리주의 약독화

박정우,장경수,정용성,김귀현,송희종,전무형 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

In the cases of Newcastle disease(ND) outbreaks in the pheasant farms located in Chungchung area, five heat-stable Newcastle disease viruses(NDV) have been isolated from the diseased pheasants, and the various biological properities of the isolates have been reported. In this study, we attempted attenuation of a heat-stable NDV isolate, CBPI by serial subpassages in SPF chick-embryo and examined its pathogenicity and immunogenicity. When CBPI isolate was subpassaged by the 153rd levels, the heat stability was not changed as compared with that of the parent virus. However, its virulence was reduced according to increasing level of subpassage. The chicken infected with the attenuated CBPI at age of 1, 2 and 5 weeks showed no mortality with mild respiratory signs in all age groups tested, and significantly decreasing body weight gains in the 5 week-old chicken, while parent virus showed 100% mortality in all age groups. In comparison of adverse reactions between the attenuated CBPI and B1 strain in 1 week-old chicken with NDV maternal antibody(HI antibody titer>1:8). both viruses manifested no specific clinical signs, but significantly lower body weight gain in the group of chicken inoculated with CBPI. When the attenuated CBPI or B1 strains were inoculated into the 3 week-old chicken vaccinated primarily with B1 strain, both of viruses showed no specific side effects and changes in body weight. However, the HI antibody titers in the chicken inoculated with the attenuated CBPI were slightly higher than that of the chicken inoculated with B1 vaccine.

감마선 조사에 의한 갈색 새우(Penaeus aztecus) 주요알러젠(Pen a 1)의 알러지성 및 항원성의 변화

이주운,김재훈,성창근,강근옥,신명곤,변명우,Lee, Ju-Woon,Kim, Jae-Hun,Sung, Chang-Keun,Kang, Kun-Ok,Shin, Myung-Gon,Byun, Myung-Woo 한국식품과학회 2000 한국식품과학회지 Vol.32 No.4

새우 알러지를 감소시키기 위한 방법으로써 감마선 조사기술의 이용 가능성을 평가하였다. 새우로부터 분리한 열안정성 단백질(HSP)과 병원에서 임상진단용으로 사용하는 새우 단백질 추출액을 1, 3, 5, 7, 10 kGy의 선량으로 감마선을 조사하였다. 단백질 추출액 및 HSP의 알러지성과 항원성의 변화를 mAb와 환자의 IgE를 사용한 ELISA법과 immunoblotting법으로 측정하였고, 단백질의 구조변화는 gel permeation chromatography와 SDS-PAGE로 관찰하였다. 환자의 IgE는 감마선 조사된 단백질 추출액과 HSP용액 모두에서 감마선 조사선량이 증가함에 따라 감소하였다. GPC-HPLC와 sandwich ELISA법으로 HSP를 정량하였을 때도 역시 감마선 조사선량이 증가함에 따라 감소하였다. SDS-PAGE pattern 변화에서 HSP는 감마선 조사에 의해 변화되어 더 큰 분자량으로 전환되었다. 이러한 결과는 감마선 조사가 새우 알러지 억제에 적용 할 수 있다는 가능성을 시사하였다. Gamma irradiation was applied to reduce shrimp allergy. Shrimp heat-stable protein(HSP) and shrimp protein extract were gamma-irradiated at 1, 3, 5, 7 or 10 kGy in an aqueous state (1.0 mg/mL). The changes in allergenic and antigenic properties of protein extract and HSP resulted from gamma irradiation were monitored by ELISA with mouse mAb or human patients sera and immunoblotting. Conformational changes in irradiated HSP were measured by both GPC-HPLC and SDS-PAGE. The binding ability of shrimp allergic patients IgE to irradiated protein extract or irradiated heat-stable protein was dose-dependently reduced. When measured by gel permeation chromatography and sandwich ELISA, the amount of intact heat-stable protein in the irradiated solution was reduced by gamma irradiation depending upon the applied dose. SDS-PAGE showed that the main band disappeared and new bands appeared in a higher molecular weight zone. The results provide a new possibility to use irradiation process for reducing the allergenicity of shrimp.

포유자돈 소장에서 분리된 대장균의 섬모항원과 장내독소 분포양상

함희진,천두성,채찬희,Ham, Hee-jin,Cheon, Doo-sung,Chae, Chan-hee 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.4

A comprehensive study of 132 Escherichia coli isolates from 150 piglets with colibacillosis included detection of heat-labile enterotoxin, heat-stable enterotoxin, and identification of K88 (F4), K99 (F5), 987P (F6), and F41. Four pili were examined by haemagglutination and slide agglutination test. Heat-labile(LT) and heat-stable(ST) enterotoxin was determined by reverse passive latex agglutination and precipitation test, respectively. Among 132 E coli isolates, 26 had K88 (19.7%), 16 had K99 (12.1%), 3 had 987P (2.3%), and 2 had F41 (1.5%). Three had K88 and K99 (2.3%), 3 had K88 and 987P (2.3%), 2 had K99 and 987P (1.5%), 5 had K99 and F41 (3.8%), and 8 E coli strains had K88, K99 and F41 (6.1%) simultaneously. Among 132 E coli isolates, 5 produced LT only (3.8%), 55 produced heat-stable toxin ST only (41.7%), and 4 produced both LT and ST (3.0%). Three major pathotypes accounted for 27.9% of E coli isolates: $K99^+$ (8.3%), $K88^+ST^+$ (9%) and $K88^+$ (10.6%). Results of this study indicated that piliated enterotoxin-producing E coli was prevalent and was associated with diarrhea in preweaning piglets.

초고온 archaeon인 Thermococcus profundus에서 P93 복합체의 분리 및 구조적 특성

이미홍,김숙경,윤영근,박성철,박정동,정강원,Lee, Mi-Hong,Kim, Suk-Kyoung,Yun, Young-Gun,Park, Seong-Cheol,Bahk, Jeong-Dong,Cheong, Gang-Won 한국현미경학회 2000 Applied microscopy Vol.30 No.2

초고온 archaeon인 Thermococcus profundus에서 매우 거대한 단백질 복합체를 분리 및 구조를 규명하였다. 거대 복합체는 93kDa단백질(P93 complex)로 구성된 homomultimer이며, 강한 내열성을 보여주고 있다. 순수 분리한 P93 complex를 SDS(최종 농도 1%)와 $85^{\circ}C$에서 12시간 항온시킨 후, SDS-PAGE와 전자현미경에서 구조적 변화를 관찰할 수 없었다. 음착색된 P93 complex의 전자현미경 사진에서 하나의 형태만을 보여주고 있으며, 구조의 규명을 위해 image processing을 하였다. 이의 구조는 3대칭 중심에 core(혹은 hole)이 뚜렷이 존재하며 이를 중심으로 단백질이 모여 있는 형태를 보여 주고 있다. 또한 P93 complex는 가장자리에서 뚜렷한 형태를 보여주지 않은 부분, 즉 flexible부분을 포함하고 있다. Gel filtration과 2차원 구조를 기초로 P93 complex는 24 homomultimer로 되어 있음을 추정하였다. An unusually large protein complex was found in the cytosol of the hyperthmophilic archaeon. Thermococcus profundus. The purified protein was shown to be a homomultimer of 93 kDa subunit (P93 complex). The complex is extremely heat stable. During 12 hrs incubation with SDS (final concentration 1%) at $85^{\circ}C$, no changed structure could be observed. Electron image analysis of negatively stained showed that the complex has a single, stable characteristic view and a well-preserved core with threefold rotational symmetry. The periphery of the assembly is composed of a nebulose, possibly flexible, component. Based on the projected structure suggest the P93 complex from T. profundus is composed 24 homomultimer.