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      • KCI등재

        저온 보존을 이용한 간편 중기 식물캘러스 저장법

        박성철,박수현,김소영,정유정,김차영,정재철 한국식물생명공학회 2022 JOURNAL OF PLANT BIOTECHNOLOGY Vol.49 No.3

        The repeated monthly or weekly subculture of plant callus is labor intensive and increases the risk of somaclonal variation from the parental callus line. The most effective method for preserving plant callus is cryo- preservation, which involves storage in liquid nitrogen. However, this method cannot be applied to the callus of different plant species in the same manner, so it is difficult to develop a standardized cryopreservation method. In addition, the survival rate of the frozen callus after thawing and the regeneration rate after survival are uncertain. Therefore, it is necessary to develop a method to extend the subculture interval of plant callus in an active state. In this study, active plant calli of various species without freezing was incubated at 15°C for 4 to 12 weeks without subculture. After 12 weeks, 8 lines of plant callus grew less than 2-fold when cultured at 25°C, but at least 2 times as much when cultured at 15°C. Moreover, total antioxidant activity did not differ significantly between plant callus recovered at 25°C after culturing at 15°C or at 25°C. These results show that the subculture interval can be extended at a temperature of 15°C without need for modified medium composition or additional processes. In addition, positive results in all calli of several plant species are expected to reduce labor as well as somaclonal variation by increasing the subculture.

      • KCI등재

        CBR-Genetic Algorithm Based Design Team Selection Model for Large-Scale Design Firms

        박성철,구교진 대한토목학회 2011 KSCE JOURNAL OF CIVIL ENGINEERING Vol.15 No.7

        Design has always played an important role in building projects, as it affects the construction process. Selecting competent design teams is a key factor that needs to be considered for the successful completion of a design project. Applying only the limited experience and subjective judgment of humans can have a negative effect on the process of decision-making, since it is difficult to sufficiently consider all the influential factors affecting the options for a combination of teams. Therefore, it is necessary to develop an automated model to effectively support experiences and judgments of decision-makers. In this context, this paper employs Case Base Reasoning(CBR) based on the solutions of similar past cases along with Genetic Algorithm(GA) generating useful solutions through combinations of each individual. The CBR-Genetic Algorithm based design team selection model used for selecting appropriate design teams was developed in this study based on a literature review and an analysis of current selection processes. The developed CBR-Genetic Algorithm based design team selection model, which consists of Modules I and II, was then validated by comparing the results obtained from twelve experts with the results that were automatically selected by Module I and II through real case studies (50 multi-family completed apartment design projects). It was shown that the CBR-Genetic Algorithm based design team selection model is superior to current selection processes because it is able to select appropriate design teams tailored for a future project by simultaneously considering numerous criteria and a variety of combinations of design team possibilities.

      • KCI등재

        Evidence-Based, Non-Pharmacological Treatment Guideline for Depression in Korea

        박성철,오홍석,오동훈,정승아,나경세,이화영,강이헌,최윤경,이민수,박용천 대한의학회 2014 Journal of Korean medical science Vol.29 No.1

        Although pharmacological treatment constitutes the main therapeutic approach for depression, non-pharmacological treatments (self-care or psychotherapeutic approach) are usually regarded as more essential therapeutic approaches in clinical practice. However, there have been few clinical practice guidelines concerning self-care or psychotherapy in the management of depression. This study introduces the ‘Evidence-Based, Non-Pharmacological Treatment Guideline for Depression in Korea.’ For the first time, a guideline was developed for non-pharmacological treatments for Korean adults with mild-to-moderate depression. The guideline development process consisted of establishing several key questions related to non-pharmacologic treatments of depression, searching the literature for studies which answer these questions, assessing the evidence level of each selected study, drawing up draft recommendation, and peer review. The Scottish Intercollegiate Guidelines Network grading system was used to evaluate the quality of evidence. As a result of this process, the guideline recommends exercise therapy, bibliotherapy, cognitive behavior therapy, short-term psychodynamic supportive psychotherapy, and interpersonal psychotherapy as the non-pharmacological treatments for adult patients with mild-to-moderate depression in Korea. Hence, it is necessary to develop specific methodologies for several non-pharmacological treatment for Korean adults with depression.

      • 韓國家族計劃實踐率의 推移 (1965-1967) : 1965 to 1967

        朴成轍 서울大學校 保健大學院 1969 公衆保健雜誌 Vol.6 No.2

        The following summary is from an analysis of time trends of contraceptive practice with Korean women(774 women were interviewed in 1965, 914 in 1966, and 1016 in 1967). 1. Time trends in contraceptive practice: The percent of women who have ever practiced contraception has risen steadily since 1965, from 22.4% to 27.2% to 28.0% among all eligible women in 1965, 1966 and 1967 respectively. 2. Trends in contraceptive practiced by age: The highest level of contraception occurs in the 35-39 age group in 1966 and 1967 but in the 40-44 age group in 1965. The pattern in toward younger women. 3. Trends in contraceptive practice by level of education: The higher their level of education, the more likely women are to practice contraception is both urban and rural areas. At all levels of education, however, practice in rural areas has become as high as in urban areas. 4. Trends in contraceptive practice by religionr: Christian, Buddhist and non-religious women have gradually increased contraceptive practice, while Catholics and Confucians have grdually declined in their practice ratios. Religion itself may probably be a very minor factor in substantiating behavioral changes. 5. Trends in contraceptive practice by number of living children: The mean of living children among "ever-practicing" women was 4.0,4.2 and 4.1 in 1965, 1966 and 1967 respectively, while the mean of number of living children among all respondents was 3.5, 3.4 and 3.4 in these years. Thus practicers remain the more fertile women. 6. Trends in contraceptive practice by age at first marriage: The higher the age at first marriage, the lower the practice ratio: with women marrieed under age 20 showing the highest ratio of practice. This is probably due to the fact that the age of marriage has been increasing recently, with women married at above age 20 still too young to practice much family planning. 7. Trends in contraceptive practice by duration of marriage: Practice ratios increase with duration of marriage since they increase high with parity. More women have practiced in urban areas where marriage duration was short. 8. Trends in contraceptive practice by frequency of induced abortion: The mean frequency of induced abortion for "everpracticing" woman was 0.6 and 0.7 in 1965, 1966 and 1967 respectively. On the other hand, all respondents showed rates of 0.2 and 0.3. 9. Trends in contraceptive practice by ideal family size: The mean ideal family size for ever-practicing women was 3.8 through 1967: while 3.9 was the mean among all respondents. 10. Trends in contraceptive practice by registration status: Among those currently practing women, registered women were 63.8%, 67.6%, 67.2% in 1965, 1966 and 1967 respectively.

      • KCI등재

        Algicidal effects of free-amine water-soluble chitosan to marine harmful algal species

        박성철,최창용,정경원,이현수,최선주,김원석,나재운 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.34 No.-

        Harmful algal blooms (HABs) commonly cause significant damage to aquatic systems due to toxinproduction and depletion of oxygen. In this study, the low molecular weight water-soluble chitosan(LMWSC) was investigated as a new algicidal compound by first investigating the potential of LMWSC inselective combating HABs. The LMWSCs disrupted algal cell and chloroplast membranes within 1 hagainst HABs, leading to efflux of the intracellular components. For co-cultivation of harmful andharmless algal, LMWSC selectively kill the harmful algal. Based on these results, LMWSC is a promisingnovel material for harmful algal bloom control.

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